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Protein

Pre-mRNA-splicing factor SLU7

Gene

SLU7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 135CCHC-typeAdd BLAST18

GO - Molecular functioni

  • pre-mRNA 3'-splice site binding Source: HGNC
  • second spliceosomal transesterification activity Source: HGNC
  • zinc ion binding Source: HGNC

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: HGNC
  • cellular response to heat Source: UniProtKB
  • intracellular protein transport Source: UniProtKB
  • mRNA 3'-end processing Source: Reactome
  • mRNA 3'-splice site recognition Source: HGNC
  • mRNA export from nucleus Source: Reactome
  • mRNA splicing, via spliceosome Source: Reactome
  • RNA export from nucleus Source: Reactome
  • RNA splicing, via transesterification reactions Source: HGNC
  • termination of RNA polymerase II transcription Source: Reactome

Keywordsi

Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SLU7
Short name:
hSlu7
Gene namesi
Name:SLU7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000164609.9.
HGNCiHGNC:16939. SLU7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116R → N: Abolishes nuclear localization. 1 Publication1
Mutagenesisi117K → N: Abolishes nuclear localization. 1 Publication1
Mutagenesisi120C → S: Induces a cytoplasmic localization; when associated with S-123; G-128 and S-133. 1 Publication1
Mutagenesisi123C → S: Induces a cytoplasmic localization; when associated with S-120; G-128 and S-133. 1 Publication1
Mutagenesisi125 – 126AM → VP: Does not affect nuclear localization. 1 Publication2
Mutagenesisi128H → G: Induces a cytoplasmic localization; when associated with S-120; S-123 and S-133. 1 Publication1
Mutagenesisi129K → N: Abolishes nuclear localization. 1 Publication1
Mutagenesisi133C → S: Induces a cytoplasmic localization; when associated with S-120; S-123 and G-128. 1 Publication1
Mutagenesisi136R → N: Abolishes nuclear localization. 1
Mutagenesisi166K → N: Abolishes nuclear localization. 1 Publication1

Organism-specific databases

DisGeNETi10569.
OpenTargetsiENSG00000164609.
PharmGKBiPA128394574.

Polymorphism and mutation databases

BioMutaiSLU7.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002891942 – 586Pre-mRNA-splicing factor SLU7Add BLAST585

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Modified residuei227PhosphoserineCombined sources1
Modified residuei235PhosphoserineCombined sources1
Cross-linki349Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki408Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO95391.
MaxQBiO95391.
PaxDbiO95391.
PeptideAtlasiO95391.
PRIDEiO95391.

PTM databases

iPTMnetiO95391.
PhosphoSitePlusiO95391.

Miscellaneous databases

PMAP-CutDBiO95391.

Expressioni

Gene expression databases

BgeeiENSG00000164609.
CleanExiHS_SLU7.
ExpressionAtlasiO95391. baseline and differential.
GenevisibleiO95391. HS.

Organism-specific databases

HPAiHPA035907.
HPA058120.

Interactioni

Subunit structurei

Component of late spliceosomal complexes. Associates with the spliceosome prior to recognition of the 3'-splice site for step II, probably during catalysis of step I.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115820. 31 interactors.
CORUMiO95391.
IntActiO95391. 34 interactors.
MINTiMINT-1467161.
STRINGi9606.ENSP00000297151.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XJCelectron microscopy3.60Z1-586[»]
ProteinModelPortaliO95391.
SMRiO95391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi129 – 169Bipartite nuclear localization signalAdd BLAST41

Domaini

The CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.1 Publication

Sequence similaritiesi

Belongs to the SLU7 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 135CCHC-typeAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2560. Eukaryota.
ENOG410XPPP. LUCA.
GeneTreeiENSGT00390000002292.
HOGENOMiHOG000194391.
HOVERGENiHBG053287.
InParanoidiO95391.
KOiK12819.
OMAiKPQTLME.
OrthoDBiEOG091G05PK.
PhylomeDBiO95391.
TreeFamiTF105691.

Family and domain databases

Gene3Di4.10.60.10. 1 hit.
InterProiView protein in InterPro
IPR021715. Slu7.
IPR036875. Znf_CCHC_sf.
PfamiView protein in Pfam
PF11708. Slu7. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATVVDAVN AAPLSGSKEM SLEEPKKMTR EDWRKKKELE EQRKLGNAPA
60 70 80 90 100
EVDEEGKDIN PHIPQYISSV PWYIDPSKRP TLKHQRPQPE KQKQFSSSGE
110 120 130 140 150
WYKRGVKENS IITKYRKGAC ENCGAMTHKK KDCFERPRRV GAKFTGTNIA
160 170 180 190 200
PDEHVQPQLM FDYDGKRDRW NGYNPEEHMK IVEEYAKVDL AKRTLKAQKL
210 220 230 240 250
QEELASGKLV EQANSPKHQW GEEEPNSQME KDHNSEDEDE DKYADDIDMP
260 270 280 290 300
GQNFDSKRRI TVRNLRIRED IAKYLRNLDP NSAYYDPKTR AMRENPYANA
310 320 330 340 350
GKNPDEVSYA GDNFVRYTGD TISMAQTQLF AWEAYDKGSE VHLQADPTKL
360 370 380 390 400
ELLYKSFKVK KEDFKEQQKE SILEKYGGQE HLDAPPAELL LAQTEDYVEY
410 420 430 440 450
SRHGTVIKGQ ERAVACSKYE EDVKIHNHTH IWGSYWKEGR WGYKCCHSFF
460 470 480 490 500
KYSYCTGEAG KEIVNSEECI INEITGEESV KKPQTLMELH QEKLKEEKKK
510 520 530 540 550
KKKKKKKHRK SSSDSDDEEK KHEKLKKALN AEEARLLHVK ETMQIDERKR
560 570 580
PYNSMYETRE PTEEEMEAYR MKRQRPDDPM ASFLGQ
Length:586
Mass (Da):68,387
Last modified:November 3, 2009 - v2
Checksum:i237B8C8014EEAB6E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032598111I → V1 PublicationCorresponds to variant dbSNP:rs17856338Ensembl.1
Natural variantiVAR_032599229M → TCombined sources3 PublicationsCorresponds to variant dbSNP:rs2961944Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF101074 mRNA. Translation: AAD13774.1.
AC091842 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61554.1.
CH471062 Genomic DNA. Translation: EAW61555.1.
BC010634 mRNA. Translation: AAH10634.1.
AY486334 mRNA. Translation: AAS59410.1.
DQ174516 mRNA. Translation: ABA08382.1.
DQ174517 mRNA. Translation: ABA08383.1.
CCDSiCCDS4352.1.
RefSeqiNP_006416.3. NM_006425.4.
XP_011532720.1. XM_011534418.2.
UniGeneiHs.435342.

Genome annotation databases

EnsembliENST00000297151; ENSP00000297151; ENSG00000164609.
GeneIDi10569.
KEGGihsa:10569.
UCSCiuc003lyg.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSLU7_HUMAN
AccessioniPrimary (citable) accession number: O95391
Secondary accession number(s): D3DQK2
, Q3LUJ0, Q3LUJ1, Q6RXQ5, Q96FM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 3, 2009
Last modified: October 25, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families