Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O95389

- WISP3_HUMAN

UniProt

O95389 - WISP3_HUMAN

Protein

WNT1-inducible-signaling pathway protein 3

Gene

WISP3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Appears to be required for normal postnatal skeletal growth and cartilage homeostasis.

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. regulation of cell growth Source: InterPro
    3. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Growth factor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WNT1-inducible-signaling pathway protein 3
    Short name:
    WISP-3
    Alternative name(s):
    CCN family member 6
    Gene namesi
    Name:WISP3
    Synonyms:CCN6
    ORF Names:UNQ462/PRO790/PRO956
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:12771. WISP3.

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Involvement in diseasei

    Progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]: Autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti78 – 781C → R in PPAC. 1 Publication
    VAR_016225

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    MIMi208230. phenotype.
    Orphaneti1159. Progressive pseudorheumatoid arthropathy of childhood.
    PharmGKBiPA37374.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 354331WNT1-inducible-signaling pathway protein 3PRO_0000014412Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi268 ↔ 305By similarity
    Disulfide bondi285 ↔ 319By similarity
    Disulfide bondi296 ↔ 335By similarity
    Disulfide bondi299 ↔ 337By similarity
    Disulfide bondi304 ↔ 341By similarity
    Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO95389.
    PRIDEiO95389.

    PTM databases

    PhosphoSiteiO95389.

    Expressioni

    Tissue specificityi

    Predominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes.

    Gene expression databases

    ArrayExpressiO95389.
    BgeeiO95389.
    CleanExiHS_WISP3.
    GenevestigatoriO95389.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000354734.

    Structurei

    3D structure databases

    ProteinModelPortaliO95389.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 11774IGFBP N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini208 – 25346TSP type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini268 – 34275CTCKPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CCN family.Curated
    Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
    Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
    Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG73349.
    HOGENOMiHOG000231462.
    HOVERGENiHBG000635.
    InParanoidiO95389.
    OMAiICNEADL.
    PhylomeDBiO95389.
    TreeFamiTF326070.

    Family and domain databases

    InterProiIPR006207. Cys_knot_C.
    IPR006208. Glyco_hormone_CN.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000867. IGFBP-like.
    IPR012395. IGFBP_CNN.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view]
    PfamiPF00007. Cys_knot. 1 hit.
    PF00219. IGFBP. 1 hit.
    PF00090. TSP_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
    SMARTiSM00041. CT. 1 hit.
    SM00121. IB. 1 hit.
    SM00209. TSP1. 1 hit.
    [Graphical view]
    SUPFAMiSSF57184. SSF57184. 1 hit.
    SSF82895. SSF82895. 1 hit.
    PROSITEiPS01225. CTCK_2. 1 hit.
    PS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS50092. TSP1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O95389-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQGLLFSTLL LAGLAQFCCR VQGTGPLDTT PEGRPGEVSD APQRKQFCHW    50
    PCKCPQQKPR CPPGVSLVRD GCGCCKICAK QPGEICNEAD LCDPHKGLYC 100
    DYSVDRPRYE TGVCAYLVAV GCEFNQVHYH NGQVFQPNPL FSCLCVSGAI 150
    GCTPLFIPKL AGSHCSGAKG GKKSDQSNCS LEPLLQQLST SYKTMPAYRN 200
    LPLIWKKKCL VQATKWTPCS RTCGMGISNR VTNENSNCEM RKEKRLCYIQ 250
    PCDSNILKTI KIPKGKTCQP TFQLSKAEKF VFSGCSSTQS YKPTFCGICL 300
    DKRCCIPNKS KMITIQFDCP NEGSFKWKML WITSCVCQRN CREPGDIFSE 350
    LKIL 354
    Length:354
    Mass (Da):39,293
    Last modified:May 1, 1999 - v1
    Checksum:i67F48D0D5C2F5EE3
    GO
    Isoform 2 (identifier: O95389-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MNKRRLLYPSGWLHGPSDM

    Show »
    Length:372
    Mass (Da):41,402
    Checksum:i449A31188D480511
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti200 – 2001N → D in AAQ88715. (PubMed:12975309)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti56 – 561Q → H Common polymorphism. 1 Publication
    Corresponds to variant rs1230345 [ dbSNP | Ensembl ].
    VAR_016224
    Natural varianti60 – 601R → C.
    Corresponds to variant rs17073260 [ dbSNP | Ensembl ].
    VAR_049567
    Natural varianti78 – 781C → R in PPAC. 1 Publication
    VAR_016225

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MNKRRLLYPSGWLHGPSDM in isoform 2. 2 PublicationsVSP_037803

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF100781 mRNA. Translation: AAC96323.1.
    AY358349 mRNA. Translation: AAQ88715.1.
    AY358350 mRNA. Translation: AAQ88716.1.
    Z99289, AL512299 Genomic DNA. Translation: CAI42331.1.
    AL512299, Z99289 Genomic DNA. Translation: CAI19998.1.
    CH471051 Genomic DNA. Translation: EAW48273.1.
    CH471051 Genomic DNA. Translation: EAW48275.1.
    BC105941 mRNA. Translation: AAI05942.1.
    CCDSiCCDS5097.1. [O95389-2]
    CCDS5098.1. [O95389-1]
    RefSeqiNP_003871.1. NM_003880.3. [O95389-1]
    NP_937882.1. NM_198239.1. [O95389-2]
    UniGeneiHs.558428.

    Genome annotation databases

    EnsembliENST00000230529; ENSP00000230529; ENSG00000112761. [O95389-1]
    ENST00000361714; ENSP00000354734; ENSG00000112761. [O95389-2]
    ENST00000368666; ENSP00000357655; ENSG00000112761. [O95389-1]
    ENST00000604763; ENSP00000473777; ENSG00000112761. [O95389-1]
    GeneIDi8838.
    KEGGihsa:8838.
    UCSCiuc003pvm.3. human. [O95389-1]
    uc003pvo.3. human. [O95389-2]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF100781 mRNA. Translation: AAC96323.1 .
    AY358349 mRNA. Translation: AAQ88715.1 .
    AY358350 mRNA. Translation: AAQ88716.1 .
    Z99289 , AL512299 Genomic DNA. Translation: CAI42331.1 .
    AL512299 , Z99289 Genomic DNA. Translation: CAI19998.1 .
    CH471051 Genomic DNA. Translation: EAW48273.1 .
    CH471051 Genomic DNA. Translation: EAW48275.1 .
    BC105941 mRNA. Translation: AAI05942.1 .
    CCDSi CCDS5097.1. [O95389-2 ]
    CCDS5098.1. [O95389-1 ]
    RefSeqi NP_003871.1. NM_003880.3. [O95389-1 ]
    NP_937882.1. NM_198239.1. [O95389-2 ]
    UniGenei Hs.558428.

    3D structure databases

    ProteinModelPortali O95389.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000354734.

    PTM databases

    PhosphoSitei O95389.

    Proteomic databases

    PaxDbi O95389.
    PRIDEi O95389.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000230529 ; ENSP00000230529 ; ENSG00000112761 . [O95389-1 ]
    ENST00000361714 ; ENSP00000354734 ; ENSG00000112761 . [O95389-2 ]
    ENST00000368666 ; ENSP00000357655 ; ENSG00000112761 . [O95389-1 ]
    ENST00000604763 ; ENSP00000473777 ; ENSG00000112761 . [O95389-1 ]
    GeneIDi 8838.
    KEGGi hsa:8838.
    UCSCi uc003pvm.3. human. [O95389-1 ]
    uc003pvo.3. human. [O95389-2 ]

    Organism-specific databases

    CTDi 8838.
    GeneCardsi GC06P112375.
    HGNCi HGNC:12771. WISP3.
    MIMi 208230. phenotype.
    603400. gene.
    neXtProti NX_O95389.
    Orphaneti 1159. Progressive pseudorheumatoid arthropathy of childhood.
    PharmGKBi PA37374.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG73349.
    HOGENOMi HOG000231462.
    HOVERGENi HBG000635.
    InParanoidi O95389.
    OMAi ICNEADL.
    PhylomeDBi O95389.
    TreeFami TF326070.

    Miscellaneous databases

    GeneWikii WNT1-inducible-signaling_pathway_protein_3.
    GenomeRNAii 8838.
    NextBioi 33172.
    PROi O95389.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95389.
    Bgeei O95389.
    CleanExi HS_WISP3.
    Genevestigatori O95389.

    Family and domain databases

    InterProi IPR006207. Cys_knot_C.
    IPR006208. Glyco_hormone_CN.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000867. IGFBP-like.
    IPR012395. IGFBP_CNN.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view ]
    Pfami PF00007. Cys_knot. 1 hit.
    PF00219. IGFBP. 1 hit.
    PF00090. TSP_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036495. IGFBP_rP_CNN. 1 hit.
    SMARTi SM00041. CT. 1 hit.
    SM00121. IB. 1 hit.
    SM00209. TSP1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57184. SSF57184. 1 hit.
    SSF82895. SSF82895. 1 hit.
    PROSITEi PS01225. CTCK_2. 1 hit.
    PS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS50092. TSP1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors."
      Pennica D., Swanson T.A., Welsh J.W., Roy M.A., Lawrence D.A., Lee J., Brush J., Taneyhill L.A., Deuel B., Lew M., Watanabe C., Cohen R.L., Melham M.F., Finley G.G., Quirke P., Goddard A.D., Hillan K.J., Gurney A.L., Botstein D., Levine A.J.
      Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Bone marrow and Fetal kidney.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    3. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. Cited for: VARIANT PPAC ARG-78, VARIANT HIS-56.

    Entry informationi

    Entry nameiWISP3_HUMAN
    AccessioniPrimary (citable) accession number: O95389
    Secondary accession number(s): Q3KR29, Q5H8W4, Q6UXH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 15, 2003
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3