O95379 (TFIP8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor alpha-induced protein 8
Short name=TNF alpha-induced protein 8

Alternative name(s):
Head and neck tumor and metastasis-related protein
MDC-3.13
NF-kappa-B-inducible DED-containing protein
Short name=NDED
SCC-S2
TNF-induced protein GG2-1

Gene names

Name:

TNFAIP8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm Ref.9.
Tissue specificity	Expressed at high levels in the spleen, lymph node, thymus, thyroid, bone marrow and placenta. Expressed at high levels both in various tumor tissues, unstimulated and cytokine-activated cultured cells. Expressed at low levels in the spinal cord, ovary, lung, adrenal glands, heart, brain, testis and skeletal muscle. Ref.1 Ref.7 Ref.9
Developmental stage	Expressed at high levels in the fetal liver, lung and kidney. Ref.7
Induction	By nuclear factor-KB (NF-KB) and TNF. Induction by TNF depends upon activation of NF-KB. Ref.7 Ref.8
Sequence similarities	Belongs to the TNFAIP8 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of apoptotic process Traceable author statement Ref.7. Source: ProtInc
Cellular_component	cytoplasm Inferred from direct assay Ref.9. Source: UniProtKB
Molecular_function	cysteine-type endopeptidase inhibitor activity involved in apoptotic process Inferred from mutant phenotype Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95379-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95379-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MHSEAEESKE → MA

Isoform 3 (identifier: O95379-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MHSEAEESKEV → M

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Tumor necrosis factor alpha-induced protein 8		PRO_0000285718
Regions
	Coiled coil	49 – 83	35	Potential
Natural variations
	Alternative sequence	1 – 11	11	MHSEAEESKEV → M in isoform 3.		VSP_024898
	Alternative sequence	1 – 10	10	MHSEAEESKE → MA in isoform 2.		VSP_024899
	Natural variant	151	1	S → C. Corresponds to variant rs3203922 [ dbSNP | Ensembl ].		VAR_032047

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D9B8F6FCF9AFC2C3	FASTA	198	23,003
	10 20 30 40 50 60 MHSEAEESKE VATDVFNSKN LAVQAQKKIL GKMVSKSIAT TLIDDTSSEV LDELYRVTRE 70 80 90 100 110 120 YTQNKKEAEK IIKNLIKTVI KLAILYRNNQ FNQDELALME KFKKKVHQLA MTVVSFHQVD 130 140 150 160 170 180 YTFDRNVLSR LLNECREMLH QIIQRHLTAK SHGRVNNVFD HFSDCEFLAA LYNPFGNFKP 190 HLQKLCDGIN KMLDEENI
	Isoform 2 [UniParc]. Checksum: 0DCD250217A1209C	FASTA	190	22,048
	10 20 30 40 50 60 MAVATDVFNS KNLAVQAQKK ILGKMVSKSI ATTLIDDTSS EVLDELYRVT REYTQNKKEA 70 80 90 100 110 120 EKIIKNLIKT VIKLAILYRN NQFNQDELAL MEKFKKKVHQ LAMTVVSFHQ VDYTFDRNVL 130 140 150 160 170 180 SRLLNECREM LHQIIQRHLT AKSHGRVNNV FDHFSDCEFL AALYNPFGNF KPHLQKLCDG 190 INKMLDEENI
	Isoform 3 [UniParc]. Checksum: 60452EC0A684F535	FASTA	188	21,877
	10 20 30 40 50 60 MATDVFNSKN LAVQAQKKIL GKMVSKSIAT TLIDDTSSEV LDELYRVTRE YTQNKKEAEK 70 80 90 100 110 120 IIKNLIKTVI KLAILYRNNQ FNQDELALME KFKKKVHQLA MTVVSFHQVD YTFDRNVLSR 130 140 150 160 170 180 LLNECREMLH QIIQRHLTAK SHGRVNNVFD HFSDCEFLAA LYNPFGNFKP HLQKLCDGIN KMLDEENI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vascular endothelial genes that are responsive to tumor necrosis factor-alpha in vitro are expressed in atherosclerotic lesions, including inhibitor of apoptosis protein-1, stannin, and two novel genes." Horrevoets A.J.G., Fontijn R.D., van Zonneveld A.J., de Vries C.J.M., ten Cate J.W., Pannekoek H. Blood 93:3418-3431(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY. Tissue: Umbilical vein.
[2]	"Identification of cellular factors involved in the differentiation of dendritic cells." Nietfeld W., Meyerhans A.F. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Placenta.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Urinary bladder.
[7]	"Identification of a novel tumor necrosis factor-alpha-inducible gene, SCC-S2, containing the consensus sequence of a death effector domain of fas-associated death domain-like interleukin-1beta-converting enzyme-inhibitory protein." Kumar D., Whiteside T.L., Kasid U. J. Biol. Chem. 275:2973-2978(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 99-198 (ISOFORM 3), IDENTIFICATION, FUNCTION, INDUCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Heart.
[8]	"Nuclear factor-kappa B-inducible death effector domain-containing protein suppresses tumor necrosis factor-mediated apoptosis by inhibiting caspase-8 activity." You Z., Ouyang H., Lopatin D., Polver P.J., Wang C.-Y. J. Biol. Chem. 276:26398-26404(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[9]	"Expression of SCC-S2, an antiapoptotic molecule, correlates with enhanced proliferation and tumorigenicity of MDA-MB 435 cells." Kumar D., Gokhale P., Broustas C., Chakravarty D., Ahmad I., Kasid U. Oncogene 23:612-616(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF070671 mRNA. Translation: AAC83229.1. AF099935 mRNA. Translation: AAC72975.1. AF099936 mRNA. Translation: AAC72976.1. CR457137 mRNA. Translation: CAG33418.1. AK001850 mRNA. Translation: BAG50983.1. AK001931 mRNA. Translation: BAG50994.1. CH471086 Genomic DNA. Translation: EAW48914.1. BC005352 mRNA. Translation: AAH05352.1. BC007014 mRNA. Translation: AAH07014.1. AF098933 mRNA. Translation: AAF29435.1.
IPI	IPI00027627. IPI00807579. IPI00965514.
RefSeq	NP_001071122.1. NM_001077654.1. NP_055165.2. NM_014350.2.
UniGene	Hs.656274.
3D structure databases
ProteinModelPortal	O95379.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95379. 1 interaction.
MINT	MINT-1473332.
STRING	9606.ENSP00000408534.
Proteomic databases
PaxDb	O95379.
PRIDE	O95379.
Protocols and materials databases
DNASU	25816.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274456; ENSP00000274456; ENSG00000145779. ENST00000503646; ENSP00000421848; ENSG00000145779. ENST00000504771; ENSP00000422245; ENSG00000145779.
GeneID	25816.
KEGG	hsa:25816.
UCSC	uc003ksg.3. human. uc003ksi.3. human.
Organism-specific databases
CTD	25816.
GeneCards	GC05P118604.
H-InvDB	HIX0005115.
HGNC	HGNC:17260. TNFAIP8.
MIM	612111. gene.
neXtProt	NX_O95379.
PharmGKB	PA134957136.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG81900.
HOVERGEN	HBG053286.
InParanoid	O95379.
OrthoDB	EOG4PRSRT.
Gene expression databases
ArrayExpress	O95379.
Bgee	O95379.
CleanEx	HS_TNFAIP8.
Genevestigator	O95379.
Family and domain databases
InterPro	IPR008477. DUF758. [Graphical view]
PANTHER	PTHR12757. PTHR12757. 1 hit.
Pfam	PF05527. DUF758. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	25816.
NextBio	47047.
SOURCE	Search...

Entry information

Entry name

TFIP8_HUMAN

Accession

Primary (citable) accession number: O95379
Secondary accession number(s): B3KMH1 , B3KMI2, Q9P1Q1, Q9UER5, Q9UP47

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.