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O95376

- ARI2_HUMAN

UniProt

O95376 - ARI2_HUMAN

Protein

E3 ubiquitin-protein ligase ARIH2

Gene

ARIH2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.3 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri139 – 18850RING-type 1; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri208 – 27063IBR-typeAdd
    BLAST
    Zinc fingeri297 – 32630RING-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. protein binding Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: UniProtKB
    5. zinc ion binding Source: ProtInc

    GO - Biological processi

    1. developmental cell growth Source: UniProtKB
    2. hematopoietic stem cell proliferation Source: UniProtKB
    3. multicellular organismal development Source: ProtInc
    4. protein K48-linked ubiquitination Source: UniProtKB
    5. protein K63-linked ubiquitination Source: UniProtKB
    6. protein polyubiquitination Source: UniProtKB
    7. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase ARIH2 (EC:6.3.2.-)
    Short name:
    ARI-2
    Short name:
    Protein ariadne-2 homolog
    Alternative name(s):
    Triad1 protein
    Gene namesi
    Name:ARIH2
    Synonyms:ARI2, TRIAD1
    ORF Names:HT005
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:690. ARIH2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi158 – 1581H → A: Loss of effect in myelopoiesis. 1 Publication
    Mutagenesisi161 – 1611C → A: Loss of effect in myelopoiesis. 1 Publication

    Organism-specific databases

    PharmGKBiPA24983.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493E3 ubiquitin-protein ligase ARIH2PRO_0000055755Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei353 – 3531Phosphoserine1 Publication

    Post-translational modificationi

    Ubiquitinated. Ubiquitination promotes proteasomal degradation.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO95376.
    PaxDbiO95376.
    PRIDEiO95376.

    PTM databases

    PhosphoSiteiO95376.

    Expressioni

    Tissue specificityi

    Widely expressed with higher expression in granulocytes.1 Publication

    Inductioni

    Up-regulated by all-trans retinoic acid (ATRA). Up-regulated during differentiation of immature blood cells toward monocytes and granulocytes.1 Publication

    Gene expression databases

    ArrayExpressiO95376.
    BgeeiO95376.
    CleanExiHS_ARIH2.
    GenevestigatoriO95376.

    Interactioni

    Subunit structurei

    Interacts (via RING-type 1) with UBE2L3. Interacts (via RING-type 2) with UBE2N. Interacts (via RING-type 2) with GFI1B. Interacts with GFI1; prevents its ubiquitination and proteasomal degradation.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TP53P046375EBI-711158,EBI-366083
    UBE2L3P680363EBI-711158,EBI-711173

    Protein-protein interaction databases

    BioGridi115694. 57 interactions.
    IntActiO95376. 41 interactions.
    MINTiMINT-1376284.
    STRINGi9606.ENSP00000348769.

    Structurei

    3D structure databases

    ProteinModelPortaliO95376.
    SMRiO95376. Positions 61-492.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili369 – 40032Sequence AnalysisAdd
    BLAST
    Coiled coili439 – 49254Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi4 – 7572Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi22 – 298Poly-Glu

    Domaini

    RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.

    Sequence similaritiesi

    Belongs to the RBR family. Ariadne subfamily.Curated
    Contains 1 IBR-type zinc finger.Curated
    Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri139 – 18850RING-type 1; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri208 – 27063IBR-typeAdd
    BLAST
    Zinc fingeri297 – 32630RING-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG327249.
    HOGENOMiHOG000216611.
    HOVERGENiHBG018737.
    InParanoidiO95376.
    KOiK11969.
    OMAiTHPPHHC.
    OrthoDBiEOG7CZK58.
    PhylomeDBiO95376.
    TreeFamiTF300805.

    Family and domain databases

    Gene3Di3.30.40.10. 2 hits.
    InterProiIPR002867. Znf_C6HC.
    IPR001878. Znf_CCHC.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF01485. IBR. 2 hits.
    [Graphical view]
    SMARTiSM00647. IBR. 2 hits.
    SM00184. RING. 2 hits.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    PROSITEiPS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O95376-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVDMNSQGS DSNEEDYDPN CEEEEEEEED DPGDIEDYYV GVASDVEQQG    50
    ADAFDPEEYQ FTCLTYKESE GALNEHMTSL ASVLKVSHSV AKLILVNFHW 100
    QVSEILDRYK SNSAQLLVEA RVQPNPSKHV PTSHPPHHCA VCMQFVRKEN 150
    LLSLACQHQF CRSCWEQHCS VLVKDGVGVG VSCMAQDCPL RTPEDFVFPL 200
    LPNEELREKY RRYLFRDYVE SHYQLQLCPG ADCPMVIRVQ EPRARRVQCN 250
    RCNEVFCFKC RQMYHAPTDC ATIRKWLTKC ADDSETANYI SAHTKDCPKC 300
    NICIEKNGGC NHMQCSKCKH DFCWMCLGDW KTHGSEYYEC SRYKENPDIV 350
    NQSQQAQARE ALKKYLFYFE RWENHNKSLQ LEAQTYQRIH EKIQERVMNN 400
    LGTWIDWQYL QNAAKLLAKC RYTLQYTYPY AYYMESGPRK KLFEYQQAQL 450
    EAEIENLSWK VERADSYDRG DLENQMHIAE QRRRTLLKDF HDT 493
    Length:493
    Mass (Da):57,819
    Last modified:May 1, 1999 - v1
    Checksum:i30AFFDD327B51013
    GO

    Sequence cautioni

    The sequence AAG09696.1 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti280 – 2812CA → LQ in CAA10276. (PubMed:10880484)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti24 – 241E → K.
    Corresponds to variant rs11507 [ dbSNP | Ensembl ].
    VAR_054105
    Natural varianti29 – 291E → D.
    Corresponds to variant rs34221642 [ dbSNP | Ensembl ].
    VAR_054106

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099149 mRNA. Translation: AAC82469.1.
    AJ130978 mRNA. Translation: CAA10276.1.
    AF183427 mRNA. Translation: AAG09696.1. Frameshift.
    BC000422 mRNA. Translation: AAH00422.1.
    CCDSiCCDS2780.1.
    RefSeqiNP_006312.1. NM_006321.2.
    XP_005264855.1. XM_005264798.1.
    XP_006712987.1. XM_006712924.1.
    UniGeneiHs.633601.

    Genome annotation databases

    EnsembliENST00000356401; ENSP00000348769; ENSG00000177479.
    ENST00000449376; ENSP00000403222; ENSG00000177479.
    GeneIDi10425.
    KEGGihsa:10425.
    UCSCiuc003cvb.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099149 mRNA. Translation: AAC82469.1 .
    AJ130978 mRNA. Translation: CAA10276.1 .
    AF183427 mRNA. Translation: AAG09696.1 . Frameshift.
    BC000422 mRNA. Translation: AAH00422.1 .
    CCDSi CCDS2780.1.
    RefSeqi NP_006312.1. NM_006321.2.
    XP_005264855.1. XM_005264798.1.
    XP_006712987.1. XM_006712924.1.
    UniGenei Hs.633601.

    3D structure databases

    ProteinModelPortali O95376.
    SMRi O95376. Positions 61-492.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115694. 57 interactions.
    IntActi O95376. 41 interactions.
    MINTi MINT-1376284.
    STRINGi 9606.ENSP00000348769.

    PTM databases

    PhosphoSitei O95376.

    Proteomic databases

    MaxQBi O95376.
    PaxDbi O95376.
    PRIDEi O95376.

    Protocols and materials databases

    DNASUi 10425.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000356401 ; ENSP00000348769 ; ENSG00000177479 .
    ENST00000449376 ; ENSP00000403222 ; ENSG00000177479 .
    GeneIDi 10425.
    KEGGi hsa:10425.
    UCSCi uc003cvb.3. human.

    Organism-specific databases

    CTDi 10425.
    GeneCardsi GC03P048931.
    HGNCi HGNC:690. ARIH2.
    MIMi 605615. gene.
    neXtProti NX_O95376.
    PharmGKBi PA24983.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG327249.
    HOGENOMi HOG000216611.
    HOVERGENi HBG018737.
    InParanoidi O95376.
    KOi K11969.
    OMAi THPPHHC.
    OrthoDBi EOG7CZK58.
    PhylomeDBi O95376.
    TreeFami TF300805.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    ChiTaRSi ARIH2. human.
    GeneWikii ARIH2.
    GenomeRNAii 10425.
    NextBioi 39512.
    PROi O95376.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95376.
    Bgeei O95376.
    CleanExi HS_ARIH2.
    Genevestigatori O95376.

    Family and domain databases

    Gene3Di 3.30.40.10. 2 hits.
    InterProi IPR002867. Znf_C6HC.
    IPR001878. Znf_CCHC.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    Pfami PF01485. IBR. 2 hits.
    [Graphical view ]
    SMARTi SM00647. IBR. 2 hits.
    SM00184. RING. 2 hits.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    PROSITEi PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TRIADs: a new class of proteins with a novel cysteine-rich signature."
      van der Reijden B.A., Erpelinck-Verschueren C.A.J., Loewenberg B., Jansen J.H.
      Protein Sci. 8:1557-1561(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins."
      Aguilera M., Oliveros M., Martinez-Padron M., Barbas J.A., Ferrus A.
      Genetics 155:1231-1244(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Hypothalamus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Muscle.
    5. "The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth of primary myeloid progenitor cells."
      Marteijn J.A., van Emst L., Erpelinck-Verschueren C.A., Nikoloski G., Menke A., de Witte T., Loewenberg B., Jansen J.H., van der Reijden B.A.
      Blood 106:4114-4123(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH UBE2L3, SUBCELLULAR LOCATION, UBIQUITINATION, MUTAGENESIS OF HIS-158 AND CYS-161, TISSUE SPECIFICITY, INDUCTION BY ATRA.
    6. "Gfi1 ubiquitination and proteasomal degradation is inhibited by the ubiquitin ligase Triad1."
      Marteijn J.A., van der Meer L.T., van Emst L., van Reijmersdal S., Wissink W., de Witte T., Jansen J.H., Van der Reijden B.A.
      Blood 110:3128-3135(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GFI1 AND GFI1B.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    8. Cited for: FUNCTION, INTERACTION WITH UBE2L3 AND UBE2N, SUBCELLULAR LOCATION.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARI2_HUMAN
    AccessioniPrimary (citable) accession number: O95376
    Secondary accession number(s): Q9HBZ6, Q9UEM9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3