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O95365 (ZBT7A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 7A
Alternative name(s):
Factor binding IST protein 1
Short name=FBI-1
Factor that binds to inducer of short transcripts protein 1
HIV-1 1st-binding protein 1
Leukemia/lymphoma-related factor
POZ and Krueppel erythroid myeloid ontogenic factor
Short name=POK erythroid myeloid ontogenic factor
Short name=Pokemon
TTF-I-interacting peptide 21
Short name=TIP21
Zinc finger protein 857A
Gene names
Name:ZBTB7A
Synonyms:FBI1, LRF, ZBTB7, ZNF857A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals By similarity. Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' By similarity.

Subunit structure

Interacts with BCL6 By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). Ref.5 Ref.6

Sequence similarities

Contains 1 BTB (POZ) domain.

Contains 4 C2H2-type zinc fingers.

Sequence caution

The sequence AAB58414.1 differs from that shown. Reason: Frameshift at positions 8 and 324.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 584584Zinc finger and BTB domain-containing protein 7A
PRO_0000047715

Regions

Domain34 – 10168BTB
Zinc finger382 – 40423C2H2-type 1
Zinc finger410 – 43223C2H2-type 2
Zinc finger438 – 46023C2H2-type 3
Zinc finger466 – 49025C2H2-type 4; atypical
Compositional bias175 – 21137Ala-rich
Compositional bias175 – 1817Poly-Ala
Compositional bias560 – 5656Poly-Gly

Amino acid modifications

Modified residue3371Phosphoserine Ref.7 Ref.12
Modified residue3411Phosphoserine Ref.12
Modified residue5111Phosphoserine Ref.11
Modified residue5251Phosphoserine Ref.11
Modified residue5261Phosphoserine Ref.10
Modified residue5491Phosphoserine Ref.7 Ref.12

Experimental info

Sequence conflict5541G → C in AAH84568. Ref.4

Secondary structure

....................... 584
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95365 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 50073663F3D393A0

FASTA58461,439
        10         20         30         40         50         60 
MAGGVDGPIG IPFPDHSSDI LSGLNEQRTQ GLLCDVVILV EGREFPTHRS VLAACSQYFK 

        70         80         90        100        110        120 
KLFTSGAVVD QQNVYEIDFV SAEALTALMD FAYTATLTVS TANVGDILSA ARLLEIPAVS 

       130        140        150        160        170        180 
HVCADLLDRQ ILAADAGADA GQLDLVDQID QRNLLRAKEY LEFFQSNPMN SLPPAAAAAA 

       190        200        210        220        230        240 
ASFPWSAFGA SDDDLDATKE AVAAAVAAVA AGDCNGLDFY GPGPPAERPP TGDGDEGDSN 

       250        260        270        280        290        300 
PGLWPERDED APTGGLFPPP VAPPAATQNG HYGRGGEEEA ASLSEAAPEP GDSPGFLSGA 

       310        320        330        340        350        360 
AEGEDGDGPD VDGLAASTLL QQMMSSVGRA GAAAGDSDEE SRADDKGVMD YYLKYFSGAH 

       370        380        390        400        410        420 
DGDVYPAWSQ KVEKKIRAKA FQKCPICEKV IQGAGKLPRH IRTHTGEKPY ECNICKVRFT 

       430        440        450        460        470        480 
RQDKLKVHMR KHTGEKPYLC QQCGAAFAHN YDLKNHMRVH TGLRPYQCDS CCKTFVRSDH 

       490        500        510        520        530        540 
LHRHLKKDGC NGVPSRRGRK PRVRGGAPDP SPGATATPGA PAQPSSPDAR RNGQEKHFKD 

       550        560        570        580 
EDEDEDVASP DGLGRLNVAG AGGGGDSGGG PGAATDGNFT AGLA 

« Hide

References

« Hide 'large scale' references
[1]"FBI-1, a factor that binds to the HIV-1 inducer of short transcripts (IST), is a POZ domain protein."
Morrison D.J., Pendergrast P.S., Stavropoulos P., Colmenares S.U., Kobayashi R., Hernandez N.
Nucleic Acids Res. 27:1251-1262(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Carcinoma.
[2]"cDNA encoding TTF-I interacting peptide 21 (TIP21)."
Jansa P., Grummt I.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Pancreas.
[5]"Novel BTB/POZ domain zinc-finger protein, LRF, is a potential target of the LAZ-3/BCL-6 oncogene."
Davies J.M., Hawe N., Kabarowski J., Huang Q.-H., Zhu J., Brand N.J., Leprince D., Dhordain P., Cook M., Moriss-Kay G., Zelent A.
Oncogene 18:365-375(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Role of the proto-oncogene Pokemon in cellular transformation and ARF repression."
Maeda T., Hobbs R.M., Merghoub T., Guernah I., Zelent A., Cordon-Cardo C., Teruya-Feldstein J., Pandolfi P.P.
Nature 433:278-285(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND SER-549, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-526, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-511 AND SER-525, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337; SER-341 AND SER-549, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Structure of the POZ domain of human LRF, a master regulator of oncogenesis."
Schubot F.D., Tropea J.E., Waugh D.S.
Biochem. Biophys. Res. Commun. 351:1-6(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 9-128.
[14]"Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator."
Stogios P.J., Chen L., Prive G.G.
Protein Sci. 16:336-342(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-131.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF097916 mRNA. Translation: AAC72973.1.
AF000561 mRNA. Translation: AAB58414.1. Frameshift.
CH471139 Genomic DNA. Translation: EAW69267.1.
CH471139 Genomic DNA. Translation: EAW69268.1.
CH471139 Genomic DNA. Translation: EAW69270.1.
CH471139 Genomic DNA. Translation: EAW69271.1.
CH471139 Genomic DNA. Translation: EAW69272.1.
BC084568 mRNA. Translation: AAH84568.1.
BC113511 mRNA. Translation: AAI13512.1.
CCDSCCDS12119.1.
RefSeqNP_056982.1. NM_015898.2.
XP_005259627.2. XM_005259570.2.
XP_005259628.1. XM_005259571.2.
XP_005259629.1. XM_005259572.1.
UniGeneHs.591384.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IF5X-ray2.00A9-128[»]
2NN2X-ray2.10A/B1-131[»]
ProteinModelPortalO95365.
SMRO95365. Positions 9-128, 363-484.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119488. 28 interactions.
IntActO95365. 1 interaction.
STRING9606.ENSP00000323670.

PTM databases

PhosphoSiteO95365.

Proteomic databases

MaxQBO95365.
PaxDbO95365.
PRIDEO95365.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000322357; ENSP00000323670; ENSG00000178951.
ENST00000601588; ENSP00000471865; ENSG00000178951.
GeneID51341.
KEGGhsa:51341.
UCSCuc002lzh.3. human.

Organism-specific databases

CTD51341.
GeneCardsGC19M004045.
HGNCHGNC:18078. ZBTB7A.
HPACAB037214.
MIM605878. gene.
neXtProtNX_O95365.
PharmGKBPA134885165.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000082521.
HOVERGENHBG106747.
InParanoidO95365.
KOK10494.
OMATRDRQAP.
OrthoDBEOG70W3D4.
PhylomeDBO95365.
TreeFamTF331824.

Enzyme and pathway databases

SignaLinkO95365.

Gene expression databases

BgeeO95365.
CleanExHS_ZBTB7A.
GenevestigatorO95365.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZBTB7A. human.
EvolutionaryTraceO95365.
GeneWikiZBTB7A.
GenomeRNAi51341.
NextBio54786.
PROO95365.
SOURCESearch...

Entry information

Entry nameZBT7A_HUMAN
AccessionPrimary (citable) accession number: O95365
Secondary accession number(s): D6W619 expand/collapse secondary AC list , O00456, Q14D41, Q5XG86
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM