O95361 (TRI16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tripartite motif-containing protein 16

Alternative name(s):
Estrogen-responsive B box protein

Gene names

Name:	TRIM16
Synonyms:	EBBP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the regulation of keratinocyte differentiation.
Subcellular location	Cytoplasm.
Tissue specificity	Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
Sequence similarities	Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H3 acetylation Inferred from direct assay PubMed 19147277. Source: UniProtKB histone H4 acetylation Inferred from direct assay PubMed 16636064. Source: UniProtKB positive regulation of interleukin-1 beta secretion Inferred from mutant phenotype PubMed 16575408. Source: UniProtKB positive regulation of keratinocyte differentiation Inferred from direct assay Ref.2. Source: UniProtKB positive regulation of retinoic acid receptor signaling pathway Inferred from direct assay PubMed 16636064. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 16636064PubMed 19147277. Source: UniProtKB response to growth hormone stimulus Inferred from direct assay Ref.2. Source: UniProtKB response to organophosphorus Inferred from expression pattern PubMed 16079077. Source: UniProtKB response to retinoic acid Inferred from expression pattern PubMed 16636064. Source: UniProtKB
Cellular_component	PML body Inferred from direct assay PubMed 16636064. Source: UniProtKB cytoplasm Inferred from direct assay Ref.2PubMed 16575408Ref.1. Source: UniProtKB
Molecular_function	DNA binding Inferred from direct assay PubMed 16636064. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
E2F1	Q01094	2	EBI-727384,EBI-448924
VIM	P08670	3	EBI-727384,EBI-353844

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95361-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95361-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-216: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Tripartite motif-containing protein 16		PRO_0000056222
Regions
	Domain	355 – 553	199	B30.2/SPRY
	Zinc finger	72 – 122	51	B box-type 1
	Zinc finger	126 – 165	40	B box-type 2
	Coiled coil	165 – 203	39	Potential
	Coiled coil	243 – 274	32	Potential
	Coiled coil	320 – 340	21	Potential
Natural variations
	Alternative sequence	1 – 216	216	Missing in isoform 2.		VSP_009098
	Natural variant	121	1	E → D. Corresponds to variant rs2074890 [ dbSNP | Ensembl ].		VAR_017412
	Natural variant	493	1	R → W. Corresponds to variant rs3174720 [ dbSNP | Ensembl ].		VAR_052133
	Natural variant	561	1	G → V. Ref.1 Ref.2 Ref.3 Ref.4 Corresponds to variant rs1060903 [ dbSNP | Ensembl ].		VAR_031668
Experimental info
	Sequence conflict	235	1	L → P in AAC79080. Ref.1
	Sequence conflict	235	1	L → P Ref.2
	Sequence conflict	246	1	S → N in AAC79080. Ref.1
	Sequence conflict	246	1	S → N Ref.2
	Sequence conflict	465	1	F → L in AAH01564. Ref.4
	Sequence conflict	521	1	K → N in AAC79080. Ref.1
	Sequence conflict	521	1	K → N Ref.2
	Sequence conflict	541	1	N → S in AAC79080. Ref.1
	Sequence conflict	541	1	N → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Alpha) [UniParc]. Last modified April 17, 2007. Version 3. Checksum: A6760717937D82F8	FASTA	564	63,955
	10 20 30 40 50 60 MAELDLMAPG PLPRATAQPP APLSPDSGSP SPDSGSASPV EEEDVGSSEK LGRETEEQDS 70 80 90 100 110 120 DSAEQGDPAG EGKEVLCDFC LDDTRRVKAV KSCLTCMVNY CEEHLQPHQV NIKLQSHLLT 130 140 150 160 170 180 EPVKDHNWRY CPAHHSPLSA FCCPDQQCIC QDCCQEHSGH TIVSLDAARR DKEAELQCTQ 190 200 210 220 230 240 LDLERKLKLN ENAISRLQAN QKSVLVSVSE VKAVAEMQFG ELLAAVRKAQ ANVMLFLEEK 250 260 270 280 290 300 EQAALSQANG IKAHLEYRSA EMEKSKQELE RMAAISNTVQ FLEEYCKFKN TEDITFPSVY 310 320 330 340 350 360 VGLKDKLSGI RKVITESTVH LIQLLENYKK KLQEFSKEEE YDIRTQVSAV VQRKYWTSKP 370 380 390 400 410 420 EPSTREQFLQ YAYDITFDPD TAHKYLRLQE ENRKVTNTTP WEHPYPDLPS RFLHWRQVLS 430 440 450 460 470 480 QQSLYLHRYY FEVEIFGAGT YVGLTCKGID RKGEERNSCI SGNNFSWSLQ WNGKEFTAWY 490 500 510 520 530 540 SDMETPLKAG PFRRLGVYID FPGGILSFYG VEYDTMTLVH KFACKFSEPV YAAFWLSKKE 550 560 NAIRIVDLGE EPEKPAPSLV GTAP
	Isoform 2 (Beta) [UniParc]. Checksum: 6C2EC2F9E97FEB5D	FASTA	348	40,370
	10 20 30 40 50 60 MQFGELLAAV RKAQANVMLF LEEKEQAALS QANGIKAHLE YRSAEMEKSK QELERMAAIS 70 80 90 100 110 120 NTVQFLEEYC KFKNTEDITF PSVYVGLKDK LSGIRKVITE STVHLIQLLE NYKKKLQEFS 130 140 150 160 170 180 KEEEYDIRTQ VSAVVQRKYW TSKPEPSTRE QFLQYAYDIT FDPDTAHKYL RLQEENRKVT 190 200 210 220 230 240 NTTPWEHPYP DLPSRFLHWR QVLSQQSLYL HRYYFEVEIF GAGTYVGLTC KGIDRKGEER 250 260 270 280 290 300 NSCISGNNFS WSLQWNGKEF TAWYSDMETP LKAGPFRRLG VYIDFPGGIL SFYGVEYDTM 310 320 330 340 TLVHKFACKF SEPVYAAFWL SKKENAIRIV DLGEEPEKPA PSLVGTAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The novel estrogen-responsive B box protein (EBBP) gene is tamoxifen regulated in cells expressing an estrogen receptor DNA-binding domain mutant." Liu H.-L.C., Golder-Novoselsky E., Seto M.H., Webster L., McClary J., Zajchowski D.A. Mol. Endocrinol. 12:1733-1748(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT VAL-561. Tissue: Mammary gland.
[2]	"The estrogen-responsive B box protein: a novel regulator of keratinocyte differentiation." Beer H.-D., Munding C., Dubois N., Mamie C., Hohl D., Werner S. J. Biol. Chem. 277:20740-20749(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT VAL-561.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-561.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-561. Tissue: Placenta and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF096870 mRNA. Translation: AAC79080.1. CR457136 mRNA. Translation: CAG33417.1. BC001564 mRNA. Translation: AAH01564.1. BC053514 mRNA. Translation: AAH53514.1. BC067096 mRNA. Translation: AAH67096.1.
IPI	IPI00007955. IPI00789219.
RefSeq	NP_006461.3. NM_006470.3.
UniGene	Hs.123534.
3D structure databases
ProteinModelPortal	O95361.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95361. 3 interactions.
MINT	MINT-1384373.
STRING	9606.ENSP00000338989.
PTM databases
PhosphoSite	O95361.
Proteomic databases
PaxDb	O95361.
PRIDE	O95361.
Protocols and materials databases
DNASU	10626.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336708; ENSP00000338989; ENSG00000221926. ENST00000577886; ENSP00000462903; ENSG00000221926. ENST00000578237; ENSP00000463188; ENSG00000221926.
GeneID	10626.
KEGG	hsa:10626.
UCSC	uc002gow.3. human.
Organism-specific databases
CTD	10626.
GeneCards	GC17M015531.
H-InvDB	HIX0013608.
HGNC	HGNC:17241. TRIM16.
HPA	HPA023623.
MIM	609505. gene.
neXtProt	NX_O95361.
PharmGKB	PA38215.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG149040.
HOGENOM	HOG000060240.
HOVERGEN	HBG057134.
InParanoid	O95361.
KO	K12006.
OMA	VCEECLA.
OrthoDB	EOG480HWG.
PhylomeDB	O95361.
Gene expression databases
ArrayExpress	O95361.
Bgee	O95361.
CleanEx	HS_TRIM16.
Genevestigator	O95361.
GermOnline	ENSG00000108448. Homo sapiens.
Family and domain databases
InterPro	IPR001870. B30.2/SPRY. IPR003879. Butyrophylin. IPR008985. ConA-like_lec_gl_sf. IPR006574. PRY. IPR018355. SPla/RYanodine_receptor_subgr. IPR003877. SPRY_rcpt. IPR000315. Znf_B-box. [Graphical view]
Pfam	PF00622. SPRY. 1 hit. PF00643. zf-B_box. 1 hit. [Graphical view]
PRINTS	PR01407. BUTYPHLNCDUF.
SMART	SM00336. BBOX. 2 hits. SM00589. PRY. 1 hit. SM00449. SPRY. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS50188. B302_SPRY. 1 hit. PS50119. ZF_BBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10626.
NextBio	40372.
SOURCE	Search...

Entry information

Entry name

TRI16_HUMAN

Accession

Primary (citable) accession number: O95361
Secondary accession number(s): Q6IAL8 , Q7Z6I2, Q96BE8, Q96J43

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	April 17, 2007
Last modified:	May 1, 2013
This is version 122 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.