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O95361 (TRI16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tripartite motif-containing protein 16
Alternative name(s):
Estrogen-responsive B box protein
Gene names
Name:TRIM16
Synonyms:EBBP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in the regulation of keratinocyte differentiation.

Subcellular location

Cytoplasm.

Tissue specificity

Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

Sequence similarities

Belongs to the TRIM/RBCC family.

Contains 2 B box-type zinc fingers.

Contains 1 B30.2/SPRY domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
Repeat
Zinc-finger
   LigandMetal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhistone H3 acetylation

Inferred from direct assay PubMed 19147277. Source: UniProtKB

histone H4 acetylation

Inferred from direct assay PubMed 16636064. Source: UniProtKB

positive regulation of interleukin-1 beta secretion

Inferred from mutant phenotype PubMed 16575408. Source: UniProtKB

positive regulation of keratinocyte differentiation

Inferred from direct assay Ref.2. Source: UniProtKB

positive regulation of retinoic acid receptor signaling pathway

Inferred from direct assay PubMed 16636064. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 16636064PubMed 19147277. Source: UniProtKB

response to growth hormone

Inferred from direct assay Ref.2. Source: UniProtKB

response to organophosphorus

Inferred from expression pattern PubMed 16079077. Source: UniProtKB

response to retinoic acid

Inferred from expression pattern PubMed 16636064. Source: UniProtKB

   Cellular_componentPML body

Inferred from direct assay PubMed 16636064. Source: UniProtKB

cytoplasm

Inferred from direct assay Ref.2PubMed 16575408Ref.1. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay PubMed 16636064. Source: UniProtKB

NACHT domain binding

Inferred from physical interaction PubMed 16575408. Source: UniProtKB

interleukin-1 binding

Inferred from physical interaction PubMed 16575408. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 16575408. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95361-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95361-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-216: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564Tripartite motif-containing protein 16
PRO_0000056222

Regions

Domain355 – 553199B30.2/SPRY
Zinc finger72 – 12251B box-type 1
Zinc finger126 – 16540B box-type 2
Coiled coil165 – 20339 Potential
Coiled coil243 – 27432 Potential
Coiled coil320 – 34021 Potential

Natural variations

Alternative sequence1 – 216216Missing in isoform 2.
VSP_009098
Natural variant1211E → D.
Corresponds to variant rs2074890 [ dbSNP | Ensembl ].
VAR_017412
Natural variant4931R → W.
Corresponds to variant rs3174720 [ dbSNP | Ensembl ].
VAR_052133
Natural variant5611G → V. Ref.1 Ref.2 Ref.3 Ref.4
Corresponds to variant rs1060903 [ dbSNP | Ensembl ].
VAR_031668

Experimental info

Sequence conflict2351L → P in AAC79080. Ref.1
Sequence conflict2351L → P Ref.2
Sequence conflict2461S → N in AAC79080. Ref.1
Sequence conflict2461S → N Ref.2
Sequence conflict4651F → L in AAH01564. Ref.4
Sequence conflict5211K → N in AAC79080. Ref.1
Sequence conflict5211K → N Ref.2
Sequence conflict5411N → S in AAC79080. Ref.1
Sequence conflict5411N → S Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha) [UniParc].

Last modified April 17, 2007. Version 3.
Checksum: A6760717937D82F8

FASTA56463,955
        10         20         30         40         50         60 
MAELDLMAPG PLPRATAQPP APLSPDSGSP SPDSGSASPV EEEDVGSSEK LGRETEEQDS 

        70         80         90        100        110        120 
DSAEQGDPAG EGKEVLCDFC LDDTRRVKAV KSCLTCMVNY CEEHLQPHQV NIKLQSHLLT 

       130        140        150        160        170        180 
EPVKDHNWRY CPAHHSPLSA FCCPDQQCIC QDCCQEHSGH TIVSLDAARR DKEAELQCTQ 

       190        200        210        220        230        240 
LDLERKLKLN ENAISRLQAN QKSVLVSVSE VKAVAEMQFG ELLAAVRKAQ ANVMLFLEEK 

       250        260        270        280        290        300 
EQAALSQANG IKAHLEYRSA EMEKSKQELE RMAAISNTVQ FLEEYCKFKN TEDITFPSVY 

       310        320        330        340        350        360 
VGLKDKLSGI RKVITESTVH LIQLLENYKK KLQEFSKEEE YDIRTQVSAV VQRKYWTSKP 

       370        380        390        400        410        420 
EPSTREQFLQ YAYDITFDPD TAHKYLRLQE ENRKVTNTTP WEHPYPDLPS RFLHWRQVLS 

       430        440        450        460        470        480 
QQSLYLHRYY FEVEIFGAGT YVGLTCKGID RKGEERNSCI SGNNFSWSLQ WNGKEFTAWY 

       490        500        510        520        530        540 
SDMETPLKAG PFRRLGVYID FPGGILSFYG VEYDTMTLVH KFACKFSEPV YAAFWLSKKE 

       550        560 
NAIRIVDLGE EPEKPAPSLV GTAP 

« Hide

Isoform 2 (Beta) [UniParc].

Checksum: 6C2EC2F9E97FEB5D
Show »

FASTA34840,370

References

« Hide 'large scale' references
[1]"The novel estrogen-responsive B box protein (EBBP) gene is tamoxifen regulated in cells expressing an estrogen receptor DNA-binding domain mutant."
Liu H.-L.C., Golder-Novoselsky E., Seto M.H., Webster L., McClary J., Zajchowski D.A.
Mol. Endocrinol. 12:1733-1748(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT VAL-561.
Tissue: Mammary gland.
[2]"The estrogen-responsive B box protein: a novel regulator of keratinocyte differentiation."
Beer H.-D., Munding C., Dubois N., Mamie C., Hohl D., Werner S.
J. Biol. Chem. 277:20740-20749(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT VAL-561.
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-561.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-561.
Tissue: Placenta and Skin.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF096870 mRNA. Translation: AAC79080.1.
CR457136 mRNA. Translation: CAG33417.1.
BC001564 mRNA. Translation: AAH01564.1.
BC053514 mRNA. Translation: AAH53514.1.
BC067096 mRNA. Translation: AAH67096.1.
CCDSCCDS11171.1. [O95361-1]
RefSeqNP_006461.3. NM_006470.3. [O95361-1]
UniGeneHs.123534.

3D structure databases

ProteinModelPortalO95361.
SMRO95361. Positions 72-334, 364-536.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115870. 12 interactions.
IntActO95361. 3 interactions.
MINTMINT-1384373.
STRING9606.ENSP00000338989.

PTM databases

PhosphoSiteO95361.

Proteomic databases

MaxQBO95361.
PaxDbO95361.
PRIDEO95361.

Protocols and materials databases

DNASU10626.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336708; ENSP00000338989; ENSG00000221926. [O95361-1]
ENST00000577886; ENSP00000462903; ENSG00000221926. [O95361-2]
ENST00000578237; ENSP00000463188; ENSG00000221926. [O95361-1]
GeneID10626.
KEGGhsa:10626.
UCSCuc002gow.3. human. [O95361-1]

Organism-specific databases

CTD10626.
GeneCardsGC17M015531.
H-InvDBHIX0013608.
HGNCHGNC:17241. TRIM16.
HPAHPA023623.
MIM609505. gene.
neXtProtNX_O95361.
PharmGKBPA38215.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149040.
HOGENOMHOG000060240.
HOVERGENHBG057134.
InParanoidO95361.
KOK12006.
OMAVCEECLA.
PhylomeDBO95361.
TreeFamTF351086.

Gene expression databases

BgeeO95361.
CleanExHS_TRIM16.
GenevestigatorO95361.

Family and domain databases

Gene3D4.10.45.10. 1 hit.
InterProIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR008985. ConA-like_lec_gl_sf.
IPR006574. PRY.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
IPR000315. Znf_B-box.
[Graphical view]
PfamPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSPR01407. BUTYPHLNCDUF.
SMARTSM00336. BBOX. 2 hits.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTRIM16.
GenomeRNAi10626.
NextBio40372.
PROO95361.
SOURCESearch...

Entry information

Entry nameTRI16_HUMAN
AccessionPrimary (citable) accession number: O95361
Secondary accession number(s): Q6IAL8 expand/collapse secondary AC list , Q7Z6I2, Q96BE8, Q96J43
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: April 17, 2007
Last modified: July 9, 2014
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM