Reviewed, UniProtKB/Swiss-Prot O95352 (ATG7_HUMAN)

Names and origin

Protein names

Recommended name:
    Autophagy-related protein 7
Alternative name(s):
    APG7-like
    Ubiquitin-activating enzyme E1-like protein
      Short name=hAGP7

Gene names

Name:	ATG7
Synonyms:	APG7L

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy By similarity.
Subunit structure	Homodimer By similarity. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
Subcellular location	CytoplasmProbable.
Tissue specificity	Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Domain	The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12 By similarity.
Sequence similarities	Belongs to the ATG7 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW membrane fusion Ref.1 Traceable author statement. Source: ProtInc positive regulation of protein modification process Inferred from direct assay. Source: MGI protein amino acid lipidation Inferred from direct assay. Source: MGI protein transport Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein homodimerization activity Inferred from direct assay. Source: UniProtKB ubiquitin activating enzyme activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95352-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95352-2)

The sequence of this isoform differs from the canonical sequence as follows:
     626-652: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 703	703	Autophagy-related protein 7		PRO_0000212806
Sites
	Active site	572	1	Glycyl thioester intermediate Potential
Natural variations
	Alternative sequence	626 – 652	27	Missing in isoform 2.		VSP_013205

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: ABBD1A29A6C58356	FASTA	703	77,960
	10 20 30 40 50 60 MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP 70 80 90 100 110 120 ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT 130 140 150 160 170 180 ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL 190 200 210 220 230 240 ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY 250 260 270 280 290 300 PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK 310 320 330 340 350 360 AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL 370 380 390 400 410 420 LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR 430 440 450 460 470 480 LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES 490 500 510 520 530 540 RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF 550 560 570 580 590 600 ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG 610 620 630 640 650 660 GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS SKVLDQYERE 670 680 690 700 GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD ETI
	Isoform 2 [UniParc]. Checksum: 184E1A7A3B989524		676	75,002
	10 20 30 40 50 60 MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP 70 80 90 100 110 120 ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT 130 140 150 160 170 180 ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL 190 200 210 220 230 240 ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY 250 260 270 280 290 300 PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK 310 320 330 340 350 360 AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL 370 380 390 400 410 420 LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR 430 440 450 460 470 480 LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES 490 500 510 520 530 540 RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF 550 560 570 580 590 600 ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG 610 620 630 640 650 660 GYAIASSSDD RMNEPPTSLG LVPHQVLDQY EREGFNFLAK VFNSSHSFLE DLTGLTLLHQ 670 ETQAAEIWDM SDDETI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein." Yuan W., Stromhaug P.E., Dunn W.A. Jr. Mol. Biol. Cell 10:1353-1366(1999) [PubMed: 10233149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	The German cDNA consortium Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-703 (ISOFORM 1). Tissue: Testis.
[4]	"Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." Tanida I., Tanida-Miyake E., Nishitani T., Komatsu M., Yamazaki H., Ueno T., Kominami E. Biochem. Biophys. Res. Commun. 292:256-262(2002) [PubMed: 11890701] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." Tanida I., Tanida-Miyake E., Komatsu M., Ueno T., Kominami E. J. Biol. Chem. 277:13739-13744(2002) [PubMed: 11825910] [Abstract] Cited for: INTERACTION WITH ATG3 AND ATG12. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF094516 mRNA. Translation: AAC69630.1. BC000091 mRNA. Translation: AAH00091.1. AL122075 mRNA. Translation: CAB59250.1.
PIR	T34556.
RefSeq	NP_006386.1.
UniGene	Hs.38032
3D structure databases
HSSP	HSSP built from PDB template 1JW9 based on UniProtKB P12282.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95352.
PTM databases
PhosphoSite	O95352.
Genome annotation databases
Ensembl	ENSG00000197548. Homo sapiens. [Contig view]
GeneID	10533.
KEGG	hsa:10533.
Organism-specific databases
H-InvDB	HIX0003055.
HGNC	HGNC:16935. ATG7.
HPA	CAB018771. HPA007639.
MIM	608760. gene.
PharmGKB	PA134983397.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O95352.
HOVERGEN	O95352.
Gene expression databases
ArrayExpress	O95352.
CleanEx	HS_ATG7.
GermOnline	ENSG00000197548. Homo sapiens.
Family and domain databases
InterPro	IPR006285. E1_like_Apg7. IPR016040. NAD(P)-bd. IPR000594. ThiF_NAD_FAD_bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00899. ThiF. 1 hit. [Graphical view]
TIGRFAMs	TIGR01381. E1_like_apg7. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	39961.
SOURCE	Search...

Entry information

Entry name

ATG7_HUMAN

Accession

Primary (citable) accession number: O95352
Secondary accession number(s): Q7L8L0, Q9BWP2, Q9UFH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)