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O95292

- VAPB_HUMAN

UniProt

O95292 - VAPB_HUMAN

Protein

Vesicle-associated membrane protein-associated protein B/C

Gene

VAPB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.3 Publications

    GO - Molecular functioni

    1. beta-tubulin binding Source: UniProtKB
    2. enzyme binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein heterodimerization activity Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB
    6. structural molecule activity Source: InterPro

    GO - Biological processi

    1. activation of signaling protein activity involved in unfolded protein response Source: UniProtKB
    2. cell death Source: UniProtKB-KW
    3. cellular calcium ion homeostasis Source: UniProtKB
    4. endoplasmic reticulum unfolded protein response Source: UniProtKB
    5. modulation by virus of host morphology or physiology Source: UniProtKB
    6. positive regulation of viral genome replication Source: UniProtKB
    7. small molecule metabolic process Source: Reactome
    8. sphingolipid biosynthetic process Source: Reactome
    9. sphingolipid metabolic process Source: Reactome

    Keywords - Biological processi

    Host-virus interaction, Unfolded protein response

    Enzyme and pathway databases

    ReactomeiREACT_115810. Sphingolipid de novo biosynthesis.

    Protein family/group databases

    TCDBi9.B.17.1.1. the vamp-associated protein (vap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-associated membrane protein-associated protein B/C
    Short name:
    VAMP-B/VAMP-C
    Short name:
    VAMP-associated protein B/C
    Short name:
    VAP-B/VAP-C
    Gene namesi
    Name:VAPB
    ORF Names:UNQ484/PRO983
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:12649. VAPB.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Single-pass type IV membrane protein By similarity
    Note: Present in mitochondria-associated membranes that are endoplasmic reticulum membrane regions closely apposed to the outer mitochondrial membrane.1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB
    3. Golgi apparatus Source: UniProtKB
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Amyotrophic lateral sclerosis 8 (ALS8) [MIM:608627]: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.2 Publications
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti46 – 461T → I in ALS8; it forms insoluble cytosolic aggregates; cannot activate the UPR pathway through ERN1/IRE1 induction; results in ubiquitinated aggregates accumulation and cell death. 1 Publication
    Corresponds to variant rs281875284 [ dbSNP | Ensembl ].
    VAR_067964
    Natural varianti56 – 561P → S in ALS8 and SMAPAD; it forms insoluble cytosolic aggregates; cannot activate the UPR pathway; affects interaction with RMDN3; affects cellular calcium homeostasis. 1 Publication
    Corresponds to variant rs74315431 [ dbSNP | Ensembl ].
    VAR_026743
    Spinal muscular atrophy, proximal, adult, autosomal dominant (SMAPAD) [MIM:182980]: A form of spinal muscular atrophy, a neuromuscular disorder characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAPAD is characterized by proximal muscle weakness that begins in the lower limbs and then progresses to upper limbs, onset in late adulthood (after third decade) and a benign course. Most of the patients remain ambulatory 10 to 40 years after clinical onset.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti56 – 561P → S in ALS8 and SMAPAD; it forms insoluble cytosolic aggregates; cannot activate the UPR pathway; affects interaction with RMDN3; affects cellular calcium homeostasis. 1 Publication
    Corresponds to variant rs74315431 [ dbSNP | Ensembl ].
    VAR_026743

    Keywords - Diseasei

    Amyotrophic lateral sclerosis, Disease mutation, Neurodegeneration

    Organism-specific databases

    MIMi182980. phenotype.
    608627. phenotype.
    Orphaneti209335. Adult-onset proximal spinal muscular atrophy, autosomal dominant.
    803. Amyotrophic lateral sclerosis.
    PharmGKBiPA37273.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 243242Vesicle-associated membrane protein-associated protein B/CPRO_0000213473Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei150 – 1501Phosphothreonine2 Publications
    Modified residuei156 – 1561Phosphoserine2 Publications
    Modified residuei160 – 1601Phosphoserine2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO95292.
    PaxDbiO95292.
    PRIDEiO95292.

    PTM databases

    PhosphoSiteiO95292.

    Expressioni

    Tissue specificityi

    Ubiquitous. Isoform 1 predominates.

    Gene expression databases

    ArrayExpressiO95292.
    BgeeiO95292.
    CleanExiHS_VAPB.
    GenevestigatoriO95292.

    Organism-specific databases

    HPAiCAB013722.
    HPA013144.

    Interactioni

    Subunit structurei

    Homodimer, and heterodimer with VAPA. Interacts with VAMP1 and VAMP2. Interacts with HCV NS5A and NS5B. Interacts (via MSP domain) with ZFYVE27. Interacts with RMDN3.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q034633EBI-9350855,EBI-8803474From a different organism.
    Epha4Q031372EBI-1188298,EBI-1539152From a different organism.
    VAPAQ9P0L02EBI-9350848,EBI-1059156
    YIF1AO950709EBI-1188298,EBI-2799703
    ZFYVE27Q5T4F42EBI-1188298,EBI-3892947

    Protein-protein interaction databases

    BioGridi114650. 36 interactions.
    IntActiO95292. 24 interactions.
    STRINGi9606.ENSP00000417175.

    Structurei

    Secondary structure

    1
    243
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni4 – 74
    Beta strandi8 – 2013
    Beta strandi26 – 338
    Beta strandi36 – 383
    Beta strandi40 – 478
    Turni49 – 513
    Beta strandi52 – 6110
    Beta strandi66 – 738
    Turni82 – 843
    Beta strandi88 – 947
    Turni104 – 1085
    Beta strandi111 – 1133
    Beta strandi115 – 12410

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2MDKNMR-A1-125[»]
    3IKKX-ray2.50A/B1-125[»]
    ProteinModelPortaliO95292.
    SMRiO95292. Positions 1-125.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95292.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 222221CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei223 – 24321Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 124118MSPPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili159 – 19638Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MSP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5066.
    HOGENOMiHOG000293182.
    HOVERGENiHBG028551.
    InParanoidiO95292.
    KOiK10707.
    OMAiPHDIEIN.
    OrthoDBiEOG7CK389.
    PhylomeDBiO95292.
    TreeFamiTF317024.

    Family and domain databases

    Gene3Di2.60.40.360. 1 hit.
    InterProiIPR000535. MSP_dom.
    IPR008962. PapD-like.
    IPR016763. Vesicle-associated_membrane.
    [Graphical view]
    PfamiPF00635. Motile_Sperm. 1 hit.
    [Graphical view]
    PIRSFiPIRSF019693. VAMP-associated. 1 hit.
    SUPFAMiSSF49354. SSF49354. 1 hit.
    PROSITEiPS50202. MSP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O95292-1) [UniParc]FASTAAdd to Basket

    Also known as: VAP-B

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR    50
    RYCVRPNSGI IDAGASINVS VMLQPFDYDP NEKSKHKFMV QSMFAPTDTS 100
    DMEAVWKEAK PEDLMDSKLR CVFELPAEND KPHDVEINKI ISTTASKTET 150
    PIVSKSLSSS LDDTEVKKVM EECKRLQGEV QRLREENKQF KEEDGLRMRK 200
    TVQSNSPISA LAPTGKEEGL STRLLALVVL FFIVGVIIGK IAL 243
    Length:243
    Mass (Da):27,228
    Last modified:January 23, 2007 - v3
    Checksum:i22AEEF9EC7FC0B3F
    GO
    Isoform 2 (identifier: O95292-2) [UniParc]FASTAAdd to Basket

    Also known as: VAP-C

    The sequence of this isoform differs from the canonical sequence as follows:
         71-99: VMLQPFDYDPNEKSKHKFMVQSMFAPTDT → GRRWTADEEDSAEQQPHFSISPNWEGRRP
         100-243: Missing.

    Show »
    Length:99
    Mass (Da):11,152
    Checksum:i042285C56B89CCDC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601I → V in AAF67013. (PubMed:10931946)Curated
    Sequence conflicti67 – 671I → L in AAF67013. (PubMed:10931946)Curated
    Sequence conflicti97 – 971T → P in AAF67013. (PubMed:10931946)Curated
    Sequence conflicti103 – 1064EAVW → DGTR in AAF67013. (PubMed:10931946)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti46 – 461T → I in ALS8; it forms insoluble cytosolic aggregates; cannot activate the UPR pathway through ERN1/IRE1 induction; results in ubiquitinated aggregates accumulation and cell death. 1 Publication
    Corresponds to variant rs281875284 [ dbSNP | Ensembl ].
    VAR_067964
    Natural varianti56 – 561P → S in ALS8 and SMAPAD; it forms insoluble cytosolic aggregates; cannot activate the UPR pathway; affects interaction with RMDN3; affects cellular calcium homeostasis. 1 Publication
    Corresponds to variant rs74315431 [ dbSNP | Ensembl ].
    VAR_026743

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei71 – 9929VMLQP…APTDT → GRRWTADEEDSAEQQPHFSI SPNWEGRRP in isoform 2. 1 PublicationVSP_003277Add
    BLAST
    Alternative sequencei100 – 243144Missing in isoform 2. 1 PublicationVSP_003278Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF086628 mRNA. Translation: AAD13577.1.
    AF086629 mRNA. Translation: AAD13578.1.
    AF160212 mRNA. Translation: AAF67013.1.
    AY358464 mRNA. Translation: AAQ88829.1.
    AL035455 Genomic DNA. Translation: CAC15021.1.
    AL035455 Genomic DNA. Translation: CAM27023.1.
    BC001712 mRNA. Translation: AAH01712.1.
    CCDSiCCDS33498.1. [O95292-1]
    CCDS56198.1. [O95292-2]
    PIRiJG0186.
    RefSeqiNP_001182606.1. NM_001195677.1. [O95292-2]
    NP_004729.1. NM_004738.4. [O95292-1]
    UniGeneiHs.182625.

    Genome annotation databases

    EnsembliENST00000395802; ENSP00000379147; ENSG00000124164. [O95292-2]
    ENST00000475243; ENSP00000417175; ENSG00000124164. [O95292-1]
    GeneIDi9217.
    KEGGihsa:9217.
    UCSCiuc002xza.3. human. [O95292-1]
    uc002xzd.2. human. [O95292-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Alsod

    ALS genetic mutations db

    Mendelian genes VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB)

    Leiden Open Variation Database (LOVD)

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF086628 mRNA. Translation: AAD13577.1 .
    AF086629 mRNA. Translation: AAD13578.1 .
    AF160212 mRNA. Translation: AAF67013.1 .
    AY358464 mRNA. Translation: AAQ88829.1 .
    AL035455 Genomic DNA. Translation: CAC15021.1 .
    AL035455 Genomic DNA. Translation: CAM27023.1 .
    BC001712 mRNA. Translation: AAH01712.1 .
    CCDSi CCDS33498.1. [O95292-1 ]
    CCDS56198.1. [O95292-2 ]
    PIRi JG0186.
    RefSeqi NP_001182606.1. NM_001195677.1. [O95292-2 ]
    NP_004729.1. NM_004738.4. [O95292-1 ]
    UniGenei Hs.182625.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2MDK NMR - A 1-125 [» ]
    3IKK X-ray 2.50 A/B 1-125 [» ]
    ProteinModelPortali O95292.
    SMRi O95292. Positions 1-125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114650. 36 interactions.
    IntActi O95292. 24 interactions.
    STRINGi 9606.ENSP00000417175.

    Protein family/group databases

    TCDBi 9.B.17.1.1. the vamp-associated protein (vap) family.

    PTM databases

    PhosphoSitei O95292.

    Proteomic databases

    MaxQBi O95292.
    PaxDbi O95292.
    PRIDEi O95292.

    Protocols and materials databases

    DNASUi 9217.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000395802 ; ENSP00000379147 ; ENSG00000124164 . [O95292-2 ]
    ENST00000475243 ; ENSP00000417175 ; ENSG00000124164 . [O95292-1 ]
    GeneIDi 9217.
    KEGGi hsa:9217.
    UCSCi uc002xza.3. human. [O95292-1 ]
    uc002xzd.2. human. [O95292-2 ]

    Organism-specific databases

    CTDi 9217.
    GeneCardsi GC20P056964.
    GeneReviewsi VAPB.
    HGNCi HGNC:12649. VAPB.
    HPAi CAB013722.
    HPA013144.
    MIMi 182980. phenotype.
    605704. gene.
    608627. phenotype.
    neXtProti NX_O95292.
    Orphaneti 209335. Adult-onset proximal spinal muscular atrophy, autosomal dominant.
    803. Amyotrophic lateral sclerosis.
    PharmGKBi PA37273.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5066.
    HOGENOMi HOG000293182.
    HOVERGENi HBG028551.
    InParanoidi O95292.
    KOi K10707.
    OMAi PHDIEIN.
    OrthoDBi EOG7CK389.
    PhylomeDBi O95292.
    TreeFami TF317024.

    Enzyme and pathway databases

    Reactomei REACT_115810. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    ChiTaRSi VAPB. human.
    EvolutionaryTracei O95292.
    GeneWikii VAPB.
    GenomeRNAii 9217.
    NextBioi 34553.
    PROi O95292.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95292.
    Bgeei O95292.
    CleanExi HS_VAPB.
    Genevestigatori O95292.

    Family and domain databases

    Gene3Di 2.60.40.360. 1 hit.
    InterProi IPR000535. MSP_dom.
    IPR008962. PapD-like.
    IPR016763. Vesicle-associated_membrane.
    [Graphical view ]
    Pfami PF00635. Motile_Sperm. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF019693. VAMP-associated. 1 hit.
    SUPFAMi SSF49354. SSF49354. 1 hit.
    PROSITEi PS50202. MSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins."
      Nishimura Y., Hayashi M., Inada H., Tanaka T.
      Biochem. Biophys. Res. Commun. 254:21-26(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Heart.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Adrenal gland.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lymph.
    6. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
      Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-19.
      Tissue: Platelet.
    7. "Human VAP-B is involved in hepatitis C virus replication through interaction with NS5A and NS5B."
      Hamamoto I., Nishimura Y., Okamoto T., Aizaki H., Liu M., Mori Y., Abe T., Suzuki T., Lai M.M., Miyamura T., Moriishi K., Matsuura Y.
      J. Virol. 79:13473-13482(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HCV NS5A AND NS5B.
    8. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Characterization of amyotrophic lateral sclerosis-linked P56S mutation of vesicle-associated membrane protein-associated protein B (VAPB/ALS8)."
      Kanekura K., Nishimoto I., Aiso S., Matsuoka M.
      J. Biol. Chem. 281:30223-30233(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ENDOPLASMIC RETICULUM UNFOLDED PROTEIN RESPONSE, CHARACTERIZATION OF VARIANT ALS8 SER-56.
    10. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-150; SER-156 AND SER-160, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    13. "Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein."
      Saita S., Shirane M., Natume T., Iemura S., Nakayama K.I.
      J. Biol. Chem. 284:13766-13777(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZFYVE27.
    14. "Characterization of the properties of a novel mutation in VAPB in familial amyotrophic lateral sclerosis."
      Chen H.J., Anagnostou G., Chai A., Withers J., Morris A., Adhikaree J., Pennetta G., de Belleroche J.S.
      J. Biol. Chem. 285:40266-40281(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ENDOPLASMIC RETICULUM UNFOLDED PROTEIN RESPONSE, VARIANT ALS8 ILE-46, CHARACTERIZATION OF VARIANTS ALS8 ILE-46 AND SER-56.
    15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-150; SER-156 AND SER-160, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis."
      De Vos K.J., Morotz G.M., Stoica R., Tudor E.L., Lau K.F., Ackerley S., Warley A., Shaw C.E., Miller C.C.
      Hum. Mol. Genet. 21:1299-1311(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CELLULAR CALCIUM HOMEOSTASIS REGULATION, SUBCELLULAR LOCATION, INTERACTION WITH RMDN3, CHARACTERIZATION OF VARIANT ALS8 SER-56.
    19. "A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis."
      Nishimura A.L., Mitne-Neto M., Silva H.C., Richieri-Costa A., Middleton S., Cascio D., Kok F., Oliveira J.R., Gillingwater T., Webb J., Skehel P., Zatz M.
      Am. J. Hum. Genet. 75:822-831(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT ALS8 SER-56, VARIANT SMAPAD SER-56.

    Entry informationi

    Entry nameiVAPB_HUMAN
    AccessioniPrimary (citable) accession number: O95292
    Secondary accession number(s): A2A2F2, O95293, Q9P0H0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3