O95279 (KCNK5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 5

Alternative name(s):
Acid-sensitive potassium channel protein TASK-2
TWIK-related acid-sensitive K(+) channel 2

Gene names

Name:	KCNK5
Synonyms:	TASK2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	pH-dependent, voltage insensitive, outwardly rectifying potassium channel. Outward rectification is lost at high external K+ concentrations.
Subunit structure	Homodimer; disulfide-linked. Ref.6
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Abundant expression in kidney, also detected in liver, placenta and small intestine. In the kidney, expression is restricted to the distal tubules and collecting ducts. Not expressed in proximal tubules or glomeruli.
Miscellaneous	Inhibited by quinine, quinidine and external acidification.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	excretion Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	potassium channel activity Traceable author statement Ref.1. Source: ProtInc voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Potassium channel subfamily K member 5		PRO_0000101749
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 26	19	Helical; Potential
	Intramembrane	85 – 112	28	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Topological domain	134 – 157	24	Cytoplasmic Potential
	Transmembrane	158 – 180	23	Helical; Potential
	Intramembrane	190 – 215	26	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	230 – 250	21	Helical; Potential
	Topological domain	251 – 325	75	Cytoplasmic Potential
Amino acid modifications
	Modified residue	371	1	Phosphoserine Ref.7
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51		Interchain Ref.6
Natural variations
	Natural variant	465	1	P → T. Corresponds to variant rs9462487 [ dbSNP | Ensembl ].		VAR_052425

Sequences

	Sequence		Length	Mass (Da)
	O95279 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E871A7A4823DDA00	FASTA	499	55,130
	10 20 30 40 50 60 MVDRGPLLTS AIIFYLAIGA AIFEVLEEPH WKEAKKNYYT QKLHLLKEFP CLGQEGLDKI 70 80 90 100 110 120 LEVVSDAAGQ GVAITGNQTF NNWNWPNAMI FAATVITTIG YGNVAPKTPA GRLFCVFYGL 130 140 150 160 170 180 FGVPLCLTWI SALGKFFGGR AKRLGQFLTK RGVSLRKAQI TCTVIFIVWG VLVHLVIPPF 190 200 210 220 230 240 VFMVTEGWNY IEGLYYSFIT ISTIGFGDFV AGVNPSANYH ALYRYFVELW IYLGLAWLSL 250 260 270 280 290 300 FVNWKVSMFV EVHKAIKKRR RRRKESFESS PHSRKALQVK GSTASKDVNI FSFLSKKEET 310 320 330 340 350 360 YNDLIKQIGK KAMKTSGGGE TGPGPGLGPQ GGGLPALPPS LVPLVVYSKN RVPTLEEVSQ 370 380 390 400 410 420 TLRSKGHVSR SPDEEAVARA PEDSSPAPEV FMNQLDRISE ECEPWDAQDY HPLIFQDASI 430 440 450 460 470 480 TFVNTEAGLS DEETSKSSLE DNLAGEESPQ QGAEAKAPLN MGEFPSSSES TFTSTESELS 490 VPYEQLMNEY NKANSPKGT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a novel pH-sensitive two pore domain K+ channel from human kidney." Reyes R., Duprat F., Lesage F., Fink M., Salinas M., Farman N., Lazdunski M. J. Biol. Chem. 273:30863-30869(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Regulation of two-pore-domain (K2P) potassium leak channels by the tyrosine kinase inhibitor genistein." Gierten J., Ficker E., Bloehs R., Schlomer K., Kathofer S., Scholz E., Zitron E., Kiesecker C., Bauer A., Becker R., Katus H.A., Karle C.A., Thomas D. Br. J. Pharmacol. 154:1680-1690(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	"Extracellular conserved cysteine forms an intersubunit disulphide bridge in the KCNK5 (TASK-2) K+ channel without having an essential effect upon activity." Niemeyer M.I., Cid L.P., Valenzuela X., Paeile V., Sepulveda F.V. Mol. Membr. Biol. 20:185-191(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERCHAIN DISULFIDE BOND.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-371, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF084830 mRNA. Translation: AAC79458.1. EU978936 mRNA. Translation: ACH86095.1. AL451185 Genomic DNA. Translation: CAH70466.1. CH471081 Genomic DNA. Translation: EAX03979.1. BC060793 mRNA. Translation: AAH60793.1. BC069573 mRNA. Translation: AAH69573.1.
IPI	IPI00029507.
RefSeq	NP_003731.1. NM_003740.3.
UniGene	Hs.444448.
3D structure databases
ProteinModelPortal	O95279.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95279. 1 interaction.
Protein family/group databases
TCDB	1.A.1.8.2. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	O95279.
Proteomic databases
PaxDb	O95279.
PRIDE	O95279.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359534; ENSP00000352527; ENSG00000164626.
GeneID	8645.
KEGG	hsa:8645.
UCSC	uc003oon.3. human.
Organism-specific databases
CTD	8645.
GeneCards	GC06M039203.
HGNC	HGNC:6280. KCNK5.
MIM	603493. gene.
neXtProt	NX_O95279.
PharmGKB	PA30062.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG323264.
HOGENOM	HOG000113211.
HOVERGEN	HBG052236.
InParanoid	O95279.
KO	K04916.
OMA	PYEQLMN.
OrthoDB	EOG44J2HX.
PhylomeDB	O95279.
Gene expression databases
Bgee	O95279.
CleanEx	HS_KCNK5.
Genevestigator	O95279.
GermOnline	ENSG00000164626. Homo sapiens.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR003092. 2pore_dom_K_chnl_TASK. IPR013099. Ion_trans_2. [Graphical view]
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PRINTS	PR01333. 2POREKCHANEL. PR01095. TASKCHANNEL.
ProtoNet	Search...
Other
ChiTaRS	KCNK5. human.
GenomeRNAi	8645.
NextBio	32411.
SOURCE	Search...

Entry information

Entry name

KCNK5_HUMAN

Accession

Primary (citable) accession number: O95279
Secondary accession number(s): B5TJL2, Q5VV76

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.