Reviewed, UniProtKB/Swiss-Prot O95274 (LYPD3_HUMAN)

Names and originHide

Protein names

Recommended name:
Ly6/PLAUR domain-containing protein 3

Alternative name(s):
GPI-anchored metastasis-associated protein C4.4A homolog
Matrigel-induced gene C4 protein
Short name=MIG-C4

Gene names

Name:	LYPD3
Synonyms:	C4.4A
ORF Names:	UNQ491/PRO1007

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributesHide

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)Hide

Function	Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. Ref.1 Ref.2 Ref.6 Ref.7
Subunit structure	Binds laminin-1 and laminin-5. Interacts with LGALS3 By similarity. Interacts with AGR2 and AGR3. Ref.7
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Ref.6.
Tissue specificity	Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. Ref.2 Ref.6 Ref.7
Induction	Up-regulated in migrating keratinocytes during epithelisation of incisional skin wounds.
Post-translational modification	N-glycosylated and O-glycosylated. Ref.6
Sequence similarities	Contains 2 UPAR/Ly6 domains.
Mass spectrometry	Molecular mass is 21442.9 Da from positions 1 - 204. Determined by MALDI. Ref.6

OntologiesHide

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	anchored to plasma membrane Ref.6 Traceable author statement. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 326	296	Ly6/PLAUR domain-containing protein 3		PRO_0000226751
	Propeptide	327 – 346	20	Removed in mature form Potential		PRO_0000226752
Regions
	Domain	33 – 126	94	UPAR/Ly6 1
	Domain	140 – 222	83	UPAR/Ly6 2
Amino acid modifications
	Lipidation	326	1	GPI-anchor amidated cysteine Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Potential

SequencesHide

	Sequence		Length	Mass (Da)
	O95274-1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 97FF9B4A554934FF	FASTA	346	35,971
	10 20 30 40 50 60 MDPARKAGAQ AMIWTAGWLL LLLLRGGAQA LECYSCVQKA DDGCSPNKMK TVKCAPGVDV 70 80 90 100 110 120 CTEAVGAVET IHGQFSLAVR GCGSGLPGKN DRGLDLHGLL AFIQLQQCAQ DRCNAKLNLT 130 140 150 160 170 180 SRALDPAGNE SAYPPNGVEC YSCVGLSREA CQGTSPPVVS CYNASDHVYK GCFDGNVTLT 190 200 210 220 230 240 AANVTVSLPV RGCVQDEFCT RDGVTGPGFT LSGSCCQGSR CNSDLRNKTY FSPRIPPLVR 250 260 270 280 290 300 LPPPEPTTVA STTSVTTSTS APVRPTSTTK PMPAPTSQTP RQGVEHEASR DEEPRLTGGA 310 320 330 340 AGHQDRSNSG QYPAKGGPQQ PHNKGCVAPT AGLAALLLAV AAGVLL

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of genes involved in human urothelial cell-matrix interactions: implications for the progression pathways of malignant urothelium." Smith B.A., Kennedy W.J., Harnden P., Selby P.J., Trejdosiewicz L.K., Southgate J. Cancer Res. 61:1678-1685(2001) [PubMed: 11245483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Urothelium.
[2]	"Cloning of the human homologue of the metastasis-associated rat C4.4A." Wuerfel J., Seiter S., Stassar M., Claas A., Klaes R., Roesel M., Marhaba R., Savelyeva L., Schwab M., Matzku S., Zoeller M. Gene 262:35-41(2001) [PubMed: 11179665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Placenta.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[6]	"Structural analysis and tissue localization of human C4.4A: a protein homologue of the urokinase receptor." Hansen L.V., Gaardsvoll H., Nielsen B.S., Lund L.R., Danoe K., Jensen O.N., Ploug M. Biochem. Eng. J. 380:845-857(2004) [PubMed: 15012588] [Abstract] Cited for: PROTEIN SEQUENCE OF 196-216, FUNCTION, TISSUE SPECIFICITY, MASS SPECTROMETRY, GLYCOSYLATION, GPI-ANCHOR.
[7]	"hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan." Fletcher G.C., Patel S., Tyson K., Adam P.J., Schenker M., Loader J.A., Daviet L., Legrain P., Parekh R., Harris A.L., Terrett J.A. Br. J. Cancer 88:579-585(2003) [PubMed: 12592373] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH AGR2 AND AGR3.
+	Additional computationally mapped references.

Web resourcesHide

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	AF082889 mRNA. Translation: AAD13751.1. AJ223603 mRNA. Translation: CAA11469.2. AY359006 mRNA. Translation: AAQ89365.1. AC018758 Genomic DNA. Translation: AAG09062.1. BC039167 mRNA. Translation: AAH39167.1.
IPI	IPI00004310.
RefSeq	NP_055215.2.
UniGene	Hs.631594
3D structure databases
SMR	O95274. Positions 25-228.
ModBase	Search...
Protein-protein interaction databases
STRING	O95274.
Proteomic databases
PRIDE	O95274.
Genome annotation databases
Ensembl	ENST00000244333; ENSP00000244333; ENSG00000124466; Homo sapiens. [Genome view]
GeneID	27076.
KEGG	hsa:27076.
UCSC	uc002owl.1. human.
Organism-specific databases
CTD	27076.
GeneCards	GC19M048660.
H-InvDB	HIX0015185.
HGNC	HGNC:24880. LYPD3.
MIM	609484. gene.
PharmGKB	PA142671490.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15092.
HOGENOM	HBG126856.
HOVERGEN	HBG053008.
InParanoid	O95274.
OMA	WTTGWLL.
OrthoDB	EOG9K6JQC.
PhylomeDB	O95274.
Gene expression databases
ArrayExpress	O95274.
Bgee	O95274.
CleanEx	HS_LYPD3.
Genevestigator	O95274.
GermOnline	ENSG00000124466. Homo sapiens.
Family and domain databases
InterPro	IPR016054. LY6_UPA_recep-like. IPR001526. LY6_UPAR. [Graphical view]
Pfam	PF00021. UPAR_LY6. 2 hits. [Graphical view]
SMART	SM00134. LU. 1 hit. [Graphical view]
PROSITE	PS00983. LY6_UPAR. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	49677.
SOURCE	Search...

Entry informationHide

Entry name

LYPD3_HUMAN

Accession

Primary (citable) accession number: O95274
Secondary accession number(s): Q9UJ74

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	July 13, 2010
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.