Reviewed, UniProtKB/Swiss-Prot O95273 (CCDB1_HUMAN)

Names and origin

Protein names

Recommended name:
    Cyclin-D1-binding protein 1
Alternative name(s):
    Grap2 and cyclin-D-interacting protein
    Human homolog of Maid

Gene names

Name:	CCNDBP1
Synonyms:	DIP1, GCIP, HHM

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F-dependent transcription.
Subunit structure	Interacts with CCND1 and GRAP2. May also interact with COPS5, RPLP0, SIRT6, SYF2 and TCF3.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	Ubiquitously expressed. Expression is down-regulated in a variety of tumor types including breast, colon, prostate and rectal tumors, and is up-regulated in certain hepatic carcinomas.
Developmental stage	Expression may increase during differentiation.
Induction	Expression is induced by sodium butyrate, an inhibitor of colon cancer cell proliferation.
Post-translational modification	Phosphorylated.
Sequence similarities	Belongs to the CCNDBP1 family.
Sequence caution	The sequence AAD11777.1 differs from that shown. Reason: Frameshift at positions 8, 14 and 18. The sequence AAP97163.1 differs from that shown. Reason: Frameshift at positions 17 and 40.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: MGI
Molecular function	protein binding Ref.12 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COPS5	Q92905	1	EBI-748961,EBI-594661
TCF3	P15923-1	2	EBI-748961,EBI-769645

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95273-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95273-2)

The sequence of this isoform differs from the canonical sequence as follows:
     308-309: SA → EP
     310-360: Missing.

Notes: No experimental confirmation available.

Isoform 3 (identifier: O95273-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.

Isoform 4 (identifier: O95273-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
     308-333: SAKLVSVLKKALEITKASHVTPQPED → VSTGFEGIATEQMGRISLITSISCK
     334-360: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Cyclin-D1-binding protein 1		PRO_0000323372
Regions
	Region	1 – 208	208	Required for interaction with CCND1
	Region	1 – 190	190	Interaction with RPLP0
	Region	1 – 184	184	Interaction with TCF3
	Region	150 – 360	211	Interaction with TCF3
	Region	240 – 360	121	Interaction with RPLP0
Natural variations
	Alternative sequence	1 – 128	128	Missing in isoform 3 and isoform 4.		VSP_032012
	Alternative sequence	308 – 333	26	SAKLV…PQPED → VSTGFEGIATEQMGRISLIT SISCK in isoform 4.		VSP_032013
	Alternative sequence	308 – 309	2	SA → EP in isoform 2.		VSP_032014
	Alternative sequence	310 – 360	51	Missing in isoform 2.		VSP_032015
	Alternative sequence	334 – 360	27	Missing in isoform 4.		VSP_032016
Experimental info
	Sequence conflict	40	1	A → D in AAD11777. Ref.1
	Sequence conflict	41	1	Q → R in AAP97163. Ref.4
	Sequence conflict	74	1	F → S in BAC11433. Ref.8
	Sequence conflict	211	1	N → S in BAC11433. Ref.8
	Sequence conflict	235	1	E → G in BAF83396. Ref.7
	Sequence conflict	267	1	K → R in BAC87645. Ref.7
	Sequence conflict	274	1	L → M in AAD11777. Ref.1
	Sequence conflict	274	1	L → M in AAF67182. Ref.3
	Sequence conflict	310	1	K → R in BAC11530. Ref.7
	Sequence conflict	334	1	S → I in BAF83354. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 40C7C3686632F6A7	FASTA	360	40,262
	10 20 30 40 50 60 MASATAPAAA VPTLASPLEQ LRHLAEELRL LLPRVRVGEA QETTEEFNRE MFWRRLNEAA 70 80 90 100 110 120 VTVSREATTL TIVFSQLPLP SPQETQKFCE QVHAAIKAFI AVYYLLPKDQ GITLRKLVRG 130 140 150 160 170 180 ATLDIVDGMA QLMEVLSVTP TQSPENNDLI SYNSVWVACQ QMPQIPRDNK AAALLMLTKN 190 200 210 220 230 240 VDFVKDAHEE MEQAVEECDP YSGLLNDTEE NNSDNHNHED DVLGFPSNQD LYWSEDDQEL 250 260 270 280 290 300 IIPCLALVRA SKACLKKIRM LVAENGKKDQ VAQLDDIVDI SDEISPSVDD LALSIYPPMC 310 320 330 340 350 360 HLTVRINSAK LVSVLKKALE ITKASHVTPQ PEDSWIPLLI NAIDHCMNRI KELTQSELEL
	Isoform 2 [UniParc]. Checksum: AD95CB0FFA840BED		309	34,553
	10 20 30 40 50 60 MASATAPAAA VPTLASPLEQ LRHLAEELRL LLPRVRVGEA QETTEEFNRE MFWRRLNEAA 70 80 90 100 110 120 VTVSREATTL TIVFSQLPLP SPQETQKFCE QVHAAIKAFI AVYYLLPKDQ GITLRKLVRG 130 140 150 160 170 180 ATLDIVDGMA QLMEVLSVTP TQSPENNDLI SYNSVWVACQ QMPQIPRDNK AAALLMLTKN 190 200 210 220 230 240 VDFVKDAHEE MEQAVEECDP YSGLLNDTEE NNSDNHNHED DVLGFPSNQD LYWSEDDQEL 250 260 270 280 290 300 IIPCLALVRA SKACLKKIRM LVAENGKKDQ VAQLDDIVDI SDEISPSVDD LALSIYPPMC HLTVRINEP
	Isoform 3 [UniParc]. Checksum: 358E6410BEF74DB4		232	26,038
	10 20 30 40 50 60 MAQLMEVLSV TPTQSPENND LISYNSVWVA CQQMPQIPRD NKAAALLMLT KNVDFVKDAH 70 80 90 100 110 120 EEMEQAVEEC DPYSGLLNDT EENNSDNHNH EDDVLGFPSN QDLYWSEDDQ ELIIPCLALV 130 140 150 160 170 180 RASKACLKKI RMLVAENGKK DQVAQLDDIV DISDEISPSV DDLALSIYPP MCHLTVRINS 190 200 210 220 230 AKLVSVLKKA LEITKASHVT PQPEDSWIPL LINAIDHCMN RIKELTQSEL EL
	Isoform 4 [UniParc]. Checksum: BA2C46B96005794A		204	22,714
	10 20 30 40 50 60 MAQLMEVLSV TPTQSPENND LISYNSVWVA CQQMPQIPRD NKAAALLMLT KNVDFVKDAH 70 80 90 100 110 120 EEMEQAVEEC DPYSGLLNDT EENNSDNHNH EDDVLGFPSN QDLYWSEDDQ ELIIPCLALV 130 140 150 160 170 180 RASKACLKKI RMLVAENGKK DQVAQLDDIV DISDEISPSV DDLALSIYPP MCHLTVRINV 190 200 STGFEGIATE QMGRISLITS ISCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of DIP1, a novel protein that is related to the Id family of proteins." Yao Y., Doki Y., Jiang W., Imoto M., Venkatraj V.S., Warburton D., Santella R.M., Lu B., Yan L., Sun X.-H., Su T., Luo J., Weinstein I.B. Exp. Cell Res. 257:22-32(2000) [PubMed: 10854051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CCND1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION.
[2]	"Human homologue of maid: a dominant inhibitory helix-loop-helix protein associated with liver-specific gene expression." Terai S., Aoki H., Thorgeirsson S.S. Hepatology 32:357-366(2000) [PubMed: 10915743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH TCF3, TISSUE SPECIFICITY. Tissue: Liver.
[3]	"GCIP, a novel human grap2 and cyclin D interacting protein, regulates E2F-mediated transcriptional activity." Xia C., Bao Z., Tabassam F., Ma W., Qiu M., Hua S., Liu M. J. Biol. Chem. 275:20942-20948(2000) [PubMed: 10801854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH CCND1 AND GRAP2, TISSUE SPECIFICITY. Tissue: Liver.
[4]	"Cloning and characterization of a novel human cDNA homologous to murine Maid mRNA." Gao J., Yu L., Mao N.H., Wan Y.Z., Yang Y.M., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Hypothalamus.
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4). Tissue: Hair follicle dermal papilla, Lung, Retinoblastoma and Trachea.
[8]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreas.
[11]	"p29, a novel GCIP-interacting protein, localizes in the nucleus." Chang M.-S., Chang C.-L., Huang C.-J., Yang Y.-C. Biochem. Biophys. Res. Commun. 279:732-737(2000) [PubMed: 11118353] [Abstract] Cited for: INTERACTION WITH SYF2.
[12]	"Human homologue of maid is a useful marker protein in hepatocarcinogenesis." Takami T., Terai S., Yokoyama Y., Tanimoto H., Tajima K., Uchida K., Yamasaki T., Sakaida I., Nishina H., Thorgeirsson S.S., Okita K. Gastroenterology 128:1369-1380(2005) [PubMed: 15887118] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH COPS5.
[13]	"GCIP/CCNDBP1, a helix-loop-helix protein, suppresses tumorigenesis." Ma W., Stafford L.J., Li D., Luo J., Li X., Ning G., Liu M. J. Cell. Biochem. 100:1376-1386(2007) [PubMed: 17131381] [Abstract] Cited for: INTERACTION WITH SIRT6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[14]	"Ribosomal phosphoprotein P0 interacts with GCIP and overexpression of P0 is associated with cellular proliferation in breast and liver carcinoma cells." Chang T.-W., Chen C.-C., Chen K.-Y., Su J.-H., Chang J.-H., Chang M.-C. Oncogene 27:332-338(2008) [PubMed: 17621266] [Abstract] Cited for: INTERACTION WITH RPLP0, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF082569 mRNA. Translation: AAD11777.1. Frameshift. AF132034 mRNA. Translation: AAF77613.1. AF246144 mRNA. Translation: AAF67182.1. AF087852 mRNA. Translation: AAP97163.1. Frameshift. AF113535 mRNA. Translation: AAF14872.1. CR450331 mRNA. Translation: CAG29327.1. AK075296 mRNA. Translation: BAC11530.1. AK128849 mRNA. Translation: BAC87645.1. AK290665 mRNA. Translation: BAF83354.1. AK290707 mRNA. Translation: BAF83396.1. AK075146 mRNA. Translation: BAC11433.1. CH471125 Genomic DNA. Translation: EAW92588.1. CH471125 Genomic DNA. Translation: EAW92590.1. BC009689 mRNA. Translation: AAH09689.1.
RefSeq	NP_036274.2. NP_411241.1.
UniGene	Hs.36794
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O95273.
PTM databases
PhosphoSite	O95273.
Genome annotation databases
Ensembl	ENSG00000166946. Homo sapiens. [Contig view]
GeneID	23582.
KEGG	hsa:23582.
Organism-specific databases
HGNC	HGNC:1587. CCNDBP1.
MIM	607089. gene.
PharmGKB	PA26154.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	O95273.
Gene expression databases
ArrayExpress	O95273.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	46190.
SOURCE	Search...

Entry information

Entry name

CCDB1_HUMAN

Accession

Primary (citable) accession number: O95273
Secondary accession number(s): A8K3Q0 , A8K3U2, Q6ZQN9, Q7Z519, Q8NBS7, Q8NBY2, Q9NS19, Q9NYH3, Q9UHX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 25, 2008
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)