O95267 (GRP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RAS guanyl-releasing protein 1

Alternative name(s):
Calcium and DAG-regulated guanine nucleotide exchange factor II
Short name=CalDAG-GEFII
Ras guanyl-releasing protein

Gene names

Name:	RASGRP1
Synonyms:	RASGRP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. May also function in other cell types differentiation. Ref.6 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15
Enzyme regulation	Probably regulated by calcium By similarity. Regulated by PLC gamma, DGKA, DGKZ and by F-actin polymerization. Ref.7 Ref.8 Ref.9
Subunit structure	Forms a signaling complex with DGKZ and HRAS. Interacts with F-actin. Interacts with SKAP1. Ref.7 Ref.9 Ref.16
Subcellular location	Cytoplasm › cytosol. Cell membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Found both in the cytosol and associated with membranes. Relocalization to the cell membrane upon activation is F-actin-dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor. Localizes to somata and dendrites but not to axons of hippocampal pyramidal cells By similarity. Ref.9 Ref.10 Ref.12 Ref.13
Tissue specificity	Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level). Ref.1 Ref.3 Ref.6
Developmental stage	Expressed in fetal brain and kidney. Ref.1
Domain	The phorbol-ester/DAG-type zinc finger is the principal mediator of the targeting to membranes and is required for functional activation through DAG-binding.
Involvement in disease	Systemic lupus erythematosus (SLE) [MIM:152700]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Aberrantly spliced isoforms and/or diminished levels of RASGRP1 are found in a cohort of SLE patients raising the possibility that dysregulation of this signaling protein contributes to the development of autoimmunity in a subset of SLE patients.
Sequence similarities	Belongs to the RASGRP family. Contains 2 EF-hand domains. Contains 1 N-terminal Ras-GEF domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-GEF domain.
Sequence caution	The sequence AAH67298.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Cell membrane Cytoplasm Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Disease	Systemic lupus erythematosus
Domain	Coiled coil Repeat Zinc-finger
Ligand	Calcium Metal-binding Zinc
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Traceable author statement PubMed 9582122. Source: ProtInc cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW platelet activation Traceable author statement. Source: Reactome regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: Reactome
Molecular_function	calcium ion binding Traceable author statement PubMed 9582122. Source: ProtInc guanyl-nucleotide exchange factor activity Traceable author statement. Source: Reactome lipid binding Traceable author statement PubMed 9582122. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Several splicing events may be used independently in a modular way.

Isoform 1 (identifier: O95267-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95267-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     442-476: Missing.

Isoform 3 (identifier: O95267-3)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     442-476: Missing.
     625-632: APEEGPFT → GNKYSESR
     633-797: Missing.

Isoform 4 (identifier: O95267-4)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     442-476: Missing.
     513-516: REGL → SSGE
     517-797: Missing.

Isoform 5 (identifier: O95267-5)

Also known as: D;

The sequence of this isoform differs from the canonical sequence as follows:
     442-476: Missing.
     574-581: DCGMNCHK → GNKYSESR
     582-797: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 797	797	RAS guanyl-releasing protein 1		PRO_0000316978
Regions
	Domain	53 – 176	124	N-terminal Ras-GEF
	Domain	205 – 436	232	Ras-GEF
	Domain	470 – 505	36	EF-hand 1
	Domain	497 – 532	36	EF-hand 2
	Calcium binding	483 – 494	12	1 Potential
	Calcium binding	510 – 521	12	2 Potential
	Zinc finger	541 – 591	51	Phorbol-ester/DAG-type
	Region	57 – 110	54	Ras exchanger motif region; required for transforming activity By similarity
	Region	686 – 694	9	Suppress the PT region-mediated translocation to plasma membrane By similarity
	Region	718 – 797	80	PT region; mediates the BCR-dependent translocation to plasma membrane By similarity
	Coiled coil	746 – 786	41	Potential
Amino acid modifications
	Modified residue	184	1	Phosphothreonine; by PKC Ref.14 Ref.15
Natural variations
	Alternative sequence	442 – 476	35	Missing in isoform 2, isoform 3, isoform 4 and isoform 5.		VSP_030836
	Alternative sequence	513 – 516	4	REGL → SSGE in isoform 4.		VSP_030837
	Alternative sequence	517 – 797	281	Missing in isoform 4.		VSP_030838
	Alternative sequence	574 – 581	8	DCGMNCHK → GNKYSESR in isoform 5.		VSP_030839
	Alternative sequence	582 – 797	216	Missing in isoform 5.		VSP_030840
	Alternative sequence	625 – 632	8	APEEGPFT → GNKYSESR in isoform 3.		VSP_030841
	Alternative sequence	633 – 797	165	Missing in isoform 3.		VSP_030842
Experimental info
	Mutagenesis	271	1	R → E: Loss of function; prevents Ras activation. Ref.11
	Mutagenesis	549	1	Y → F: Loss of localization to the endoplasmic reticulum and the Golgi apparatus. Ref.10
	Sequence conflict	374	1	E → G in AAC79699. Ref.1
	Sequence conflict	374	1	E → G in AAF21898. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 8ACDCF0F715ABAA9	FASTA	797	90,402
	10 20 30 40 50 60 MGTLGKAREA PRKPSHGCRA ASKARLEAKP ANSPFPSHPS LAHITQFRMM VSLGHLAKGA 70 80 90 100 110 120 SLDDLIDSCI QSFDADGNLC RSNQLLQVML TMHRIVISSA ELLQKVITLY KDALAKNSPG 130 140 150 160 170 180 LCLKICYFVR YWITEFWVMF KMDASLTDTM EEFQELVKAK GEELHCRLID TTQINARDWS 190 200 210 220 230 240 RKLTQRIKSN TSKKRKVSLL FDHLEPEELS EHLTYLEFKS FRRISFSDYQ NYLVNSCVKE 250 260 270 280 290 300 NPTMERSIAL CNGISQWVQL MVLSRPTPQL RAEVFIKFIQ VAQKLHQLQN FNTLMAVIGG 310 320 330 340 350 360 LCHSSISRLK ETSSHVPHEI NKVLGEMTEL LSSSRNYDNY RRAYGECTDF KIPILGVHLK 370 380 390 400 410 420 DLISLYEAMP DYLEDGKVNV HKLLALYNHI SELVQLQEVA PPLEANKDLV HLLTLSLDLY 430 440 450 460 470 480 YTEDEIYELS YAREPRNHRA PPLTPSKPPV VVDWASGVSP KPDPKTISKH VQRMVDSVFK 490 500 510 520 530 540 NYDHDQDGYI SQEEFEKIAA SFPFSFCVMD KDREGLISRD EITAYFMRAS SIYSKLGLGF 550 560 570 580 590 600 PHNFQETTYL KPTFCDNCAG FLWGVIKQGY RCKDCGMNCH KQCKDLVVFE CKKRAKNPVA 610 620 630 640 650 660 PTENNTSVGP VSNLCSLGAK DLLHAPEEGP FTFPNGEAVE HGEESKDRTI MLMGVSSQKI 670 680 690 700 710 720 SLRLKRAVAH KATQTESQPW IGSEGPSGPF VLSSPRKTAQ DTLYVLPSPT SPCPSPVLVR 730 740 750 760 770 780 KRAFVKWENK DSLIKSKEEL RHLRLPTYQE LEQEINTLKA DNDALKIQLK YAQKKIESLQ 790 LEKSNHVLAQ MEQGDCS
	Isoform 2 (A) [UniParc]. Checksum: C3ACFFF5888E3236	FASTA	762	86,625
	10 20 30 40 50 60 MGTLGKAREA PRKPSHGCRA ASKARLEAKP ANSPFPSHPS LAHITQFRMM VSLGHLAKGA 70 80 90 100 110 120 SLDDLIDSCI QSFDADGNLC RSNQLLQVML TMHRIVISSA ELLQKVITLY KDALAKNSPG 130 140 150 160 170 180 LCLKICYFVR YWITEFWVMF KMDASLTDTM EEFQELVKAK GEELHCRLID TTQINARDWS 190 200 210 220 230 240 RKLTQRIKSN TSKKRKVSLL FDHLEPEELS EHLTYLEFKS FRRISFSDYQ NYLVNSCVKE 250 260 270 280 290 300 NPTMERSIAL CNGISQWVQL MVLSRPTPQL RAEVFIKFIQ VAQKLHQLQN FNTLMAVIGG 310 320 330 340 350 360 LCHSSISRLK ETSSHVPHEI NKVLGEMTEL LSSSRNYDNY RRAYGECTDF KIPILGVHLK 370 380 390 400 410 420 DLISLYEAMP DYLEDGKVNV HKLLALYNHI SELVQLQEVA PPLEANKDLV HLLTLSLDLY 430 440 450 460 470 480 YTEDEIYELS YAREPRNHRA PSVFKNYDHD QDGYISQEEF EKIAASFPFS FCVMDKDREG 490 500 510 520 530 540 LISRDEITAY FMRASSIYSK LGLGFPHNFQ ETTYLKPTFC DNCAGFLWGV IKQGYRCKDC 550 560 570 580 590 600 GMNCHKQCKD LVVFECKKRA KNPVAPTENN TSVGPVSNLC SLGAKDLLHA PEEGPFTFPN 610 620 630 640 650 660 GEAVEHGEES KDRTIMLMGV SSQKISLRLK RAVAHKATQT ESQPWIGSEG PSGPFVLSSP 670 680 690 700 710 720 RKTAQDTLYV LPSPTSPCPS PVLVRKRAFV KWENKDSLIK SKEELRHLRL PTYQELEQEI 730 740 750 760 NTLKADNDAL KIQLKYAQKK IESLQLEKSN HVLAQMEQGD CS
	Isoform 3 (B) [UniParc]. Checksum: 979AEA31D0FBE5DC	FASTA	597	68,170
	10 20 30 40 50 60 MGTLGKAREA PRKPSHGCRA ASKARLEAKP ANSPFPSHPS LAHITQFRMM VSLGHLAKGA 70 80 90 100 110 120 SLDDLIDSCI QSFDADGNLC RSNQLLQVML TMHRIVISSA ELLQKVITLY KDALAKNSPG 130 140 150 160 170 180 LCLKICYFVR YWITEFWVMF KMDASLTDTM EEFQELVKAK GEELHCRLID TTQINARDWS 190 200 210 220 230 240 RKLTQRIKSN TSKKRKVSLL FDHLEPEELS EHLTYLEFKS FRRISFSDYQ NYLVNSCVKE 250 260 270 280 290 300 NPTMERSIAL CNGISQWVQL MVLSRPTPQL RAEVFIKFIQ VAQKLHQLQN FNTLMAVIGG 310 320 330 340 350 360 LCHSSISRLK ETSSHVPHEI NKVLGEMTEL LSSSRNYDNY RRAYGECTDF KIPILGVHLK 370 380 390 400 410 420 DLISLYEAMP DYLEDGKVNV HKLLALYNHI SELVQLQEVA PPLEANKDLV HLLTLSLDLY 430 440 450 460 470 480 YTEDEIYELS YAREPRNHRA PSVFKNYDHD QDGYISQEEF EKIAASFPFS FCVMDKDREG 490 500 510 520 530 540 LISRDEITAY FMRASSIYSK LGLGFPHNFQ ETTYLKPTFC DNCAGFLWGV IKQGYRCKDC 550 560 570 580 590 GMNCHKQCKD LVVFECKKRA KNPVAPTENN TSVGPVSNLC SLGAKDLLHG NKYSESR
	Isoform 4 (C) [UniParc]. Checksum: 11408850F4E84118	FASTA	481	55,100
	10 20 30 40 50 60 MGTLGKAREA PRKPSHGCRA ASKARLEAKP ANSPFPSHPS LAHITQFRMM VSLGHLAKGA 70 80 90 100 110 120 SLDDLIDSCI QSFDADGNLC RSNQLLQVML TMHRIVISSA ELLQKVITLY KDALAKNSPG 130 140 150 160 170 180 LCLKICYFVR YWITEFWVMF KMDASLTDTM EEFQELVKAK GEELHCRLID TTQINARDWS 190 200 210 220 230 240 RKLTQRIKSN TSKKRKVSLL FDHLEPEELS EHLTYLEFKS FRRISFSDYQ NYLVNSCVKE 250 260 270 280 290 300 NPTMERSIAL CNGISQWVQL MVLSRPTPQL RAEVFIKFIQ VAQKLHQLQN FNTLMAVIGG 310 320 330 340 350 360 LCHSSISRLK ETSSHVPHEI NKVLGEMTEL LSSSRNYDNY RRAYGECTDF KIPILGVHLK 370 380 390 400 410 420 DLISLYEAMP DYLEDGKVNV HKLLALYNHI SELVQLQEVA PPLEANKDLV HLLTLSLDLY 430 440 450 460 470 480 YTEDEIYELS YAREPRNHRA PSVFKNYDHD QDGYISQEEF EKIAASFPFS FCVMDKDSSG E
	Isoform 5 (D) [UniParc]. Checksum: 54018A113532AF92	FASTA	546	62,674
	10 20 30 40 50 60 MGTLGKAREA PRKPSHGCRA ASKARLEAKP ANSPFPSHPS LAHITQFRMM VSLGHLAKGA 70 80 90 100 110 120 SLDDLIDSCI QSFDADGNLC RSNQLLQVML TMHRIVISSA ELLQKVITLY KDALAKNSPG 130 140 150 160 170 180 LCLKICYFVR YWITEFWVMF KMDASLTDTM EEFQELVKAK GEELHCRLID TTQINARDWS 190 200 210 220 230 240 RKLTQRIKSN TSKKRKVSLL FDHLEPEELS EHLTYLEFKS FRRISFSDYQ NYLVNSCVKE 250 260 270 280 290 300 NPTMERSIAL CNGISQWVQL MVLSRPTPQL RAEVFIKFIQ VAQKLHQLQN FNTLMAVIGG 310 320 330 340 350 360 LCHSSISRLK ETSSHVPHEI NKVLGEMTEL LSSSRNYDNY RRAYGECTDF KIPILGVHLK 370 380 390 400 410 420 DLISLYEAMP DYLEDGKVNV HKLLALYNHI SELVQLQEVA PPLEANKDLV HLLTLSLDLY 430 440 450 460 470 480 YTEDEIYELS YAREPRNHRA PSVFKNYDHD QDGYISQEEF EKIAASFPFS FCVMDKDREG 490 500 510 520 530 540 LISRDEITAY FMRASSIYSK LGLGFPHNFQ ETTYLKPTFC DNCAGFLWGV IKQGYRCKGN KYSESR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia." Kawasaki H., Springett G.M., Toki S., Canales J.J., Harlan P., Blumenstiel J.P., Chen E.J., Bany I.A., Mochizuki N., Ashbacher A., Matsuda M., Housman D.E., Graybiel A.M. Proc. Natl. Acad. Sci. U.S.A. 95:13278-13283(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Frontal cortex.
[2]	"RasGRP, a Ras activator: mouse and human cDNA sequences and chromosomal positions." Bottorff D.A., Ebinu J.O., Stone J.C. Mamm. Genome 10:358-361(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: T-cell.
[3]	"Defective expression of ras guanyl nucleotide-releasing protein 1 in a subset of patients with systemic lupus erythematosus." Yasuda S., Stevens R.L., Terada T., Takeda M., Hashimoto T., Fukae J., Horita T., Kataoka H., Atsumi T., Koike T. J. Immunol. 179:4890-4900(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3; 4 AND 5), INVOLVEMENT IN SYSTEMIC LUPUS ERYTHEMATOSUS, TISSUE SPECIFICITY. Tissue: Peripheral blood.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[5]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 233-797 (ISOFORM 2). Tissue: Brain.
[6]	"RasGRP links T-cell receptor signaling to Ras." Ebinu J.O., Stang S.L., Teixeira C., Bottorff D.A., Hooton J., Blumberg P.M., Barry M., Bleakley R.C., Ostergaard H.L., Stone J.C. Blood 95:3199-3203(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN T-CELL RECEPTOR SIGNALING, TOPOLOGY, TISSUE SPECIFICITY.
[7]	"Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism." Topham M.K., Prescott S.M. J. Cell Biol. 152:1135-1143(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH DGKZ AND HRAS, ENZYME REGULATION.
[8]	"Expression of a catalytically inactive form of diacylglycerol kinase alpha induces sustained signaling through RasGRP." Jones D.R., Sanjuan M.A., Stone J.C., Merida I. FASEB J. 16:595-597(2002) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION.
[9]	"Vav mediates Ras stimulation by direct activation of the GDP/GTP exchange factor Ras GRP1." Caloca M.J., Zugaza J.L., Matallanas D., Crespo P., Bustelo X.R. EMBO J. 22:3326-3336(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN B-CELLS, SUBCELLULAR LOCATION, INTERACTION WITH F-ACTIN, ENZYME REGULATION.
[10]	"Exchange factors of the RasGRP family mediate Ras activation in the Golgi." Caloca M.J., Zugaza J.L., Bustelo X.R. J. Biol. Chem. 278:33465-33473(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-549.
[11]	"Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1." Bivona T.G., Perez De Castro I., Ahearn I.M., Grana T.M., Chiu V.K., Lockyer P.J., Cullen P.J., Pellicer A., Cox A.D., Philips M.R. Nature 424:694-698(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ARG-271.
[12]	"Ras activation in Jurkat T cells following low-grade stimulation of the T-cell receptor is specific to N-Ras and occurs only on the Golgi apparatus." Perez de Castro I., Bivona T.G., Philips M.R., Pellicer A. Mol. Cell. Biol. 24:3485-3496(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]	"Inverted signaling hierarchy between RAS and RAC in T-lymphocytes." Zugaza J.L., Caloca M.J., Bustelo X.R. Oncogene 23:5823-5833(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[14]	"Phosphorylation of RasGRP3 on threonine 133 provides a mechanistic link between PKC and Ras signaling systems in B cells." Zheng Y., Liu H., Coughlin J.J., Zheng J., Li L., Stone J.C. Blood 105:3648-3654(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-184 BY PKC.
[15]	"A diacylglycerol-protein kinase C-RasGRP1 pathway directs Ras activation upon antigen receptor stimulation of T cells." Roose J.P., Mollenauer M., Gupta V.A., Stone J.C., Weiss A. Mol. Cell. Biol. 25:4426-4441(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT THR-184.
[16]	"SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1." Kosco K.A., Cerignoli F., Williams S., Abraham R.T., Mustelin T. Mol. Immunol. 45:510-522(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKAP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081195 mRNA. Translation: AAC79699.1. AF081197 mRNA. Translation: AAF21898.1. AF106071 mRNA. Translation: AAC97349.1. AY634315 mRNA. Translation: AAT47482.2. AY858556 mRNA. Translation: AAW32406.2. AY954625 mRNA. Translation: AAX54699.4. AY966005 mRNA. Translation: AAX76907.1. BC067298 mRNA. Translation: AAH67298.1. Sequence problems. BC109296 mRNA. Translation: AAI09297.1. BC109297 mRNA. Translation: AAI09298.1. AB208848 mRNA. Translation: BAD92085.1.
IPI	IPI00024733. IPI00885089. IPI00885115. IPI00885132. IPI00885149.
RefSeq	NP_001122074.1. NM_001128602.1. NP_005730.2. NM_005739.3.
UniGene	Hs.591127.
3D structure databases
HSSP	HSSP built from PDB template 1PTQ based on UniProtKB P28867.
ProteinModelPortal	O95267.
ModBase	Search...
PTM databases
PhosphoSite	O95267.
Proteomic databases
PaxDb	O95267.
PRIDE	O95267.
Protocols and materials databases
DNASU	10125.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000310803; ENSP00000310244; ENSG00000172575. ENST00000414708; ENSP00000413105; ENSG00000172575. ENST00000450598; ENSP00000388540; ENSG00000172575. ENST00000558164; ENSP00000454164; ENSG00000172575. ENST00000559830; ENSP00000452721; ENSG00000172575.
GeneID	10125.
KEGG	hsa:10125.
UCSC	uc001zkd.4. human. uc001zke.4. human.
Organism-specific databases
CTD	10125.
GeneCards	GC15M038780.
H-InvDB	HIX0038076.
HGNC	HGNC:9878. RASGRP1.
MIM	152700. phenotype. 603962. gene.
neXtProt	NX_O95267.
PharmGKB	PA34240.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG256085.
HOVERGEN	HBG007513.
InParanoid	O95267.
KO	K04350.
OMA	SEESKDR.
OrthoDB	EOG49ZXNS.
Enzyme and pathway databases
Pathway_Interaction_DB	tcrpathway. TCR signaling in naive CD4+ T cells. cd8tcrpathway. TCR signaling in naive CD8+ T cells.
Reactome	REACT_111102. Signal Transduction. REACT_604. Hemostasis. REACT_6900. Immune System.
Gene expression databases
ArrayExpress	O95267.
Bgee	O95267.
Genevestigator	O95267.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 1.10.840.10. 1 hit.
InterPro	IPR020454. DAG/PE-bd. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000651. Ras-like_Gua-exchang_fac_N. IPR008937. Ras_GEF. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. [Graphical view]
PANTHER	PTHR23113. PTHR23113. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF13405. EF_hand_4. 1 hit. PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. [Graphical view]
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 1 hit. SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 1 hit.
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 3 hits. PS00720. RASGEF. False negative. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5953.
GenomeRNAi	10125.
NextBio	38299.
SOURCE	Search...

Entry information

Entry name

GRP1_HUMAN

Accession

Primary (citable) accession number: O95267
Secondary accession number(s): Q56CZ0 , Q58G75, Q59HB1, Q5I3A8, Q6GV31, Q6NX39, Q7LDG6, Q9UI94, Q9UNN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.