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Protein

5-hydroxytryptamine receptor 3B

Gene

HTR3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

Miscellaneous

The HA-stretch region of HTR3B seems to confer increased conductance to HTR3A/HTR3B heteromers compared to that of HTR3A homomers.

GO - Molecular functioni

  • serotonin-gated cation-selective channel activity Source: GO_Central
  • transmembrane signaling receptor activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3B
Short name:
5-HT3-B
Short name:
5-HT3B
Alternative name(s):
Serotonin receptor 3B
Gene namesi
Name:HTR3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000149305.6
HGNCiHGNC:5298 HTR3B
MIMi604654 gene
neXtProtiNX_O95264

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 238ExtracellularSequence analysisAdd BLAST217
Transmembranei239 – 259Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini260 – 268CytoplasmicSequence analysis9
Transmembranei269 – 286Helical; Name=2Sequence analysisAdd BLAST18
Topological domaini287 – 303ExtracellularSequence analysisAdd BLAST17
Transmembranei304 – 324Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini325 – 414CytoplasmicSequence analysisAdd BLAST90
Transmembranei415 – 435Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini436 – 441ExtracellularSequence analysis6

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1
Mutagenesisi96N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1
Mutagenesisi138N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1
Mutagenesisi168N → S: Reduced molecular weight and cell membrane expression. 1 Publication1
Mutagenesisi203N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1

Organism-specific databases

DisGeNETi9177
OpenTargetsiENSG00000149305
PharmGKBiPA29556

Chemistry databases

ChEMBLiCHEMBL2111332
DrugBankiDB01049 Ergoloid mesylate
DB00898 Ethanol
DB13025 Tiapride
GuidetoPHARMACOLOGYi374

Polymorphism and mutation databases

BioMutaiHTR3B

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000031228922 – 4415-hydroxytryptamine receptor 3BAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi96N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi138N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi155 ↔ 169By similarity
Glycosylationi168N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi203N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylation required for membrane localization.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO95264
PeptideAtlasiO95264
PRIDEiO95264
TopDownProteomicsiO95264-1 [O95264-1]

PTM databases

iPTMnetiO95264
PhosphoSitePlusiO95264

Expressioni

Tissue specificityi

Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta.2 Publications

Gene expression databases

BgeeiENSG00000149305
CleanExiHS_HTR3B
ExpressionAtlasiO95264 baseline and differential
GenevisibleiO95264 HS

Organism-specific databases

HPAiHPA039559

Interactioni

Subunit structurei

Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex.2 Publications

Protein-protein interaction databases

IntActiO95264, 1 interactor
STRINGi9606.ENSP00000260191

Chemistry databases

BindingDBiO95264

Structurei

3D structure databases

ProteinModelPortaliO95264
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni381 – 413HA-stretchAdd BLAST33

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00910000144023
HOGENOMiHOG000241519
HOVERGENiHBG106638
InParanoidiO95264
KOiK04819
OMAiQIQFNVV
OrthoDBiEOG091G08WI
PhylomeDBiO95264
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR008132 5HT3_rcpt
IPR008134 5HT3_rcpt_B
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01710 5HT3BRECEPTR
PR01708 5HT3RECEPTOR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95264-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSSVMAPLW ACILVAAGIL ATDTHHPQDS ALYHLSKQLL QKYHKEVRPV
60 70 80 90 100
YNWTKATTVY LDLFVHAILD VDAENQILKT SVWYQEVWND EFLSWNSSMF
110 120 130 140 150
DEIREISLPL SAIWAPDIII NEFVDIERYP DLPYVYVNSS GTIENYKPIQ
160 170 180 190 200
VVSACSLETY AFPFDVQNCS LTFKSILHTV EDVDLAFLRS PEDIQHDKKA
210 220 230 240 250
FLNDSEWELL SVSSTYSILQ SSAGGFAQIQ FNVVMRRHPL VYVVSLLIPS
260 270 280 290 300
IFLMLVDLGS FYLPPNCRAR IVFKTSVLVG YTVFRVNMSN QVPRSVGSTP
310 320 330 340 350
LIGHFFTICM AFLVLSLAKS IVLVKFLHDE QRGGQEQPFL CLRGDTDADR
360 370 380 390 400
PRVEPRAQRA VVTESSLYGE HLAQPGTLKE VWSQLQSISN YLQTQDQTDQ
410 420 430 440
QEAEWLVLLS RFDRLLFQSY LFMLGIYTIT LCSLWALWGG V
Length:441
Mass (Da):50,292
Last modified:May 1, 1999 - v1
Checksum:i2ED59E4E11400648
GO
Isoform 2 (identifier: O95264-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MLSSVMAPLWACILVAA → MIVYFP

Show »
Length:430
Mass (Da):49,285
Checksum:i8C69BE27437D6D03
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037472129Y → S4 PublicationsCorresponds to variant dbSNP:rs1176744Ensembl.1
Natural variantiVAR_037473143I → T1 PublicationCorresponds to variant dbSNP:rs34550504Ensembl.1
Natural variantiVAR_037474156S → R3 PublicationsCorresponds to variant dbSNP:rs72466469Ensembl.1
Natural variantiVAR_037475183V → I3 PublicationsCorresponds to variant dbSNP:rs17116138Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0297961 – 17MLSSV…ILVAA → MIVYFP in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169255 mRNA Translation: AAF03691.1
AF080582 mRNA Translation: AAD12242.1
AM293589 mRNA Translation: CAL25321.1
EF444985 Genomic DNA Translation: ACA06001.1
AK314268 mRNA Translation: BAG36930.1
CCDSiCCDS8364.1 [O95264-1]
RefSeqiNP_006019.1, NM_006028.4 [O95264-1]
XP_011541365.1, XM_011543063.1 [O95264-2]
UniGeneiHs.241377

Genome annotation databases

EnsembliENST00000260191; ENSP00000260191; ENSG00000149305 [O95264-1]
ENST00000537778; ENSP00000443118; ENSG00000149305 [O95264-2]
GeneIDi9177
KEGGihsa:9177
UCSCiuc001pok.4 human [O95264-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry namei5HT3B_HUMAN
AccessioniPrimary (citable) accession number: O95264
Secondary accession number(s): B0YJ23, Q0VJC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health