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Protein

5-hydroxytryptamine receptor 3B

Gene

HTR3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

GO - Molecular functioni

  • serotonin-activated cation-selective channel activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000149305-MONOMER.
ReactomeiR-HSA-975298. Ligand-gated ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3B
Short name:
5-HT3-B
Short name:
5-HT3B
Alternative name(s):
Serotonin receptor 3B
Gene namesi
Name:HTR3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:5298. HTR3B.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 238ExtracellularSequence analysisAdd BLAST217
Transmembranei239 – 259Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini260 – 268CytoplasmicSequence analysis9
Transmembranei269 – 286Helical; Name=2Sequence analysisAdd BLAST18
Topological domaini287 – 303ExtracellularSequence analysisAdd BLAST17
Transmembranei304 – 324Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini325 – 414CytoplasmicSequence analysisAdd BLAST90
Transmembranei415 – 435Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini436 – 441ExtracellularSequence analysis6

GO - Cellular componenti

  • cell surface Source: CACAO
  • integral component of plasma membrane Source: Ensembl
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: InterPro
  • serotonin-activated cation-selective channel complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1
Mutagenesisi96N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1
Mutagenesisi138N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1
Mutagenesisi168N → S: Reduced molecular weight and cell membrane expression. 1 Publication1
Mutagenesisi203N → S: Reduced molecular weight. Very little expression in the cell membrane. 1 Publication1

Organism-specific databases

DisGeNETi9177.
OpenTargetsiENSG00000149305.
PharmGKBiPA29556.

Chemistry databases

ChEMBLiCHEMBL2111332.
DrugBankiDB01049. Ergoloid mesylate.
DB00898. Ethanol.

Polymorphism and mutation databases

BioMutaiHTR3B.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000031228922 – 4415-hydroxytryptamine receptor 3BAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...)1 Publication1
Glycosylationi96N-linked (GlcNAc...)1 Publication1
Glycosylationi138N-linked (GlcNAc...)1 Publication1
Disulfide bondi155 ↔ 169By similarity
Glycosylationi168N-linked (GlcNAc...)1 Publication1
Glycosylationi203N-linked (GlcNAc...)1 Publication1
Glycosylationi287N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylation required for membrane localization.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO95264.
PRIDEiO95264.
TopDownProteomicsiO95264-1. [O95264-1]

PTM databases

iPTMnetiO95264.
PhosphoSitePlusiO95264.

Expressioni

Tissue specificityi

Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta.2 Publications

Gene expression databases

BgeeiENSG00000149305.
CleanExiHS_HTR3B.
ExpressionAtlasiO95264. baseline and differential.
GenevisibleiO95264. HS.

Organism-specific databases

HPAiHPA039559.

Interactioni

Subunit structurei

Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex.2 Publications

Protein-protein interaction databases

IntActiO95264. 1 interactor.
STRINGi9606.ENSP00000260191.

Chemistry databases

BindingDBiO95264.

Structurei

3D structure databases

ProteinModelPortaliO95264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni381 – 413HA-stretchAdd BLAST33

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiO95264.
KOiK04819.
OMAiFDRLLFQ.
OrthoDBiEOG091G08WI.
PhylomeDBiO95264.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008134. 5HT3_rcpt_B.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01710. 5HT3BRECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95264-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSSVMAPLW ACILVAAGIL ATDTHHPQDS ALYHLSKQLL QKYHKEVRPV
60 70 80 90 100
YNWTKATTVY LDLFVHAILD VDAENQILKT SVWYQEVWND EFLSWNSSMF
110 120 130 140 150
DEIREISLPL SAIWAPDIII NEFVDIERYP DLPYVYVNSS GTIENYKPIQ
160 170 180 190 200
VVSACSLETY AFPFDVQNCS LTFKSILHTV EDVDLAFLRS PEDIQHDKKA
210 220 230 240 250
FLNDSEWELL SVSSTYSILQ SSAGGFAQIQ FNVVMRRHPL VYVVSLLIPS
260 270 280 290 300
IFLMLVDLGS FYLPPNCRAR IVFKTSVLVG YTVFRVNMSN QVPRSVGSTP
310 320 330 340 350
LIGHFFTICM AFLVLSLAKS IVLVKFLHDE QRGGQEQPFL CLRGDTDADR
360 370 380 390 400
PRVEPRAQRA VVTESSLYGE HLAQPGTLKE VWSQLQSISN YLQTQDQTDQ
410 420 430 440
QEAEWLVLLS RFDRLLFQSY LFMLGIYTIT LCSLWALWGG V
Length:441
Mass (Da):50,292
Last modified:May 1, 1999 - v1
Checksum:i2ED59E4E11400648
GO
Isoform 2 (identifier: O95264-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MLSSVMAPLWACILVAA → MIVYFP

Show »
Length:430
Mass (Da):49,285
Checksum:i8C69BE27437D6D03
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037472129Y → S.4 PublicationsCorresponds to variant rs1176744dbSNPEnsembl.1
Natural variantiVAR_037473143I → T.1 PublicationCorresponds to variant rs34550504dbSNPEnsembl.1
Natural variantiVAR_037474156S → R.3 PublicationsCorresponds to variant rs72466469dbSNPEnsembl.1
Natural variantiVAR_037475183V → I.3 PublicationsCorresponds to variant rs17116138dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0297961 – 17MLSSV…ILVAA → MIVYFP in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169255 mRNA. Translation: AAF03691.1.
AF080582 mRNA. Translation: AAD12242.1.
AM293589 mRNA. Translation: CAL25321.1.
EF444985 Genomic DNA. Translation: ACA06001.1.
AK314268 mRNA. Translation: BAG36930.1.
CCDSiCCDS8364.1. [O95264-1]
RefSeqiNP_006019.1. NM_006028.4. [O95264-1]
XP_011541365.1. XM_011543063.1. [O95264-2]
UniGeneiHs.241377.

Genome annotation databases

EnsembliENST00000260191; ENSP00000260191; ENSG00000149305. [O95264-1]
ENST00000537778; ENSP00000443118; ENSG00000149305. [O95264-2]
GeneIDi9177.
KEGGihsa:9177.
UCSCiuc001pok.4. human. [O95264-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169255 mRNA. Translation: AAF03691.1.
AF080582 mRNA. Translation: AAD12242.1.
AM293589 mRNA. Translation: CAL25321.1.
EF444985 Genomic DNA. Translation: ACA06001.1.
AK314268 mRNA. Translation: BAG36930.1.
CCDSiCCDS8364.1. [O95264-1]
RefSeqiNP_006019.1. NM_006028.4. [O95264-1]
XP_011541365.1. XM_011543063.1. [O95264-2]
UniGeneiHs.241377.

3D structure databases

ProteinModelPortaliO95264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO95264. 1 interactor.
STRINGi9606.ENSP00000260191.

Chemistry databases

BindingDBiO95264.
ChEMBLiCHEMBL2111332.
DrugBankiDB01049. Ergoloid mesylate.
DB00898. Ethanol.

PTM databases

iPTMnetiO95264.
PhosphoSitePlusiO95264.

Polymorphism and mutation databases

BioMutaiHTR3B.

Proteomic databases

PaxDbiO95264.
PRIDEiO95264.
TopDownProteomicsiO95264-1. [O95264-1]

Protocols and materials databases

DNASUi9177.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260191; ENSP00000260191; ENSG00000149305. [O95264-1]
ENST00000537778; ENSP00000443118; ENSG00000149305. [O95264-2]
GeneIDi9177.
KEGGihsa:9177.
UCSCiuc001pok.4. human. [O95264-1]

Organism-specific databases

CTDi9177.
DisGeNETi9177.
GeneCardsiHTR3B.
HGNCiHGNC:5298. HTR3B.
HPAiHPA039559.
MIMi604654. gene.
neXtProtiNX_O95264.
OpenTargetsiENSG00000149305.
PharmGKBiPA29556.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiO95264.
KOiK04819.
OMAiFDRLLFQ.
OrthoDBiEOG091G08WI.
PhylomeDBiO95264.
TreeFamiTF315605.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000149305-MONOMER.
ReactomeiR-HSA-975298. Ligand-gated ion channel transport.

Miscellaneous databases

GeneWikiiHTR3B.
GenomeRNAii9177.
PROiO95264.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000149305.
CleanExiHS_HTR3B.
ExpressionAtlasiO95264. baseline and differential.
GenevisibleiO95264. HS.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008134. 5HT3_rcpt_B.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01710. 5HT3BRECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei5HT3B_HUMAN
AccessioniPrimary (citable) accession number: O95264
Secondary accession number(s): B0YJ23, Q0VJC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The HA-stretch region of HTR3B seems to confer increased conductance to HTR3A/HTR3B heteromers compared to that of HTR3A homomers.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.