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O95264 (5HT3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 3B

Short name=5-HT3-B
Short name=5-HT3B
Alternative name(s):
Serotonin receptor 3B
Gene names
Name:HTR3B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. Ref.6

Subunit structure

Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex. Ref.2 Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A. Ref.8

Tissue specificity

Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta. Ref.1 Ref.2

Post-translational modification

N-glycosylation required for membrane localization.

Miscellaneous

The HA-stretch region of HTR3B seems to confer increased conductance to HTR3A/HTR3B heteromers compared to that of HTR3A homomers.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95264-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95264-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MLSSVMAPLWACILVAA → MIVYFP

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 4414205-hydroxytryptamine receptor 3B
PRO_0000312289

Regions

Topological domain22 – 238217Extracellular Potential
Transmembrane239 – 25921Helical; Name=1; Potential
Topological domain260 – 2689Cytoplasmic Potential
Transmembrane269 – 28618Helical; Name=2; Potential
Topological domain287 – 30317Extracellular Potential
Transmembrane304 – 32421Helical; Name=3; Potential
Topological domain325 – 41490Cytoplasmic Potential
Transmembrane415 – 43521Helical; Name=4; Potential
Topological domain436 – 4416Extracellular Potential
Region381 – 41333HA-stretch

Amino acid modifications

Glycosylation521N-linked (GlcNAc...) Ref.8
Glycosylation961N-linked (GlcNAc...) Ref.8
Glycosylation1381N-linked (GlcNAc...) Ref.8
Glycosylation1681N-linked (GlcNAc...) Ref.8
Glycosylation2031N-linked (GlcNAc...) Ref.8
Glycosylation2871N-linked (GlcNAc...) Potential
Disulfide bond155 ↔ 169 By similarity

Natural variations

Alternative sequence1 – 1717MLSSV…ILVAA → MIVYFP in isoform 2.
VSP_029796
Natural variant1291Y → S. Ref.3 Ref.9 Ref.10 Ref.11
Corresponds to variant rs1176744 [ dbSNP | Ensembl ].
VAR_037472
Natural variant1431I → T. Ref.11
Corresponds to variant rs34550504 [ dbSNP | Ensembl ].
VAR_037473
Natural variant1561S → R. Ref.9 Ref.10 Ref.12
Corresponds to variant rs72466469 [ dbSNP | Ensembl ].
VAR_037474
Natural variant1831V → I. Ref.9 Ref.10 Ref.11
Corresponds to variant rs17116138 [ dbSNP | Ensembl ].
VAR_037475

Experimental info

Mutagenesis521N → S: Reduced molecular weight. Very little expression in the cell membrane. Ref.8
Mutagenesis961N → S: Reduced molecular weight. Very little expression in the cell membrane. Ref.8
Mutagenesis1381N → S: Reduced molecular weight. Very little expression in the cell membrane. Ref.8
Mutagenesis1681N → S: Reduced molecular weight and cell membrane expression. Ref.8
Mutagenesis2031N → S: Reduced molecular weight. Very little expression in the cell membrane. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 2ED59E4E11400648

FASTA44150,292
        10         20         30         40         50         60 
MLSSVMAPLW ACILVAAGIL ATDTHHPQDS ALYHLSKQLL QKYHKEVRPV YNWTKATTVY 

        70         80         90        100        110        120 
LDLFVHAILD VDAENQILKT SVWYQEVWND EFLSWNSSMF DEIREISLPL SAIWAPDIII 

       130        140        150        160        170        180 
NEFVDIERYP DLPYVYVNSS GTIENYKPIQ VVSACSLETY AFPFDVQNCS LTFKSILHTV 

       190        200        210        220        230        240 
EDVDLAFLRS PEDIQHDKKA FLNDSEWELL SVSSTYSILQ SSAGGFAQIQ FNVVMRRHPL 

       250        260        270        280        290        300 
VYVVSLLIPS IFLMLVDLGS FYLPPNCRAR IVFKTSVLVG YTVFRVNMSN QVPRSVGSTP 

       310        320        330        340        350        360 
LIGHFFTICM AFLVLSLAKS IVLVKFLHDE QRGGQEQPFL CLRGDTDADR PRVEPRAQRA 

       370        380        390        400        410        420 
VVTESSLYGE HLAQPGTLKE VWSQLQSISN YLQTQDQTDQ QEAEWLVLLS RFDRLLFQSY 

       430        440 
LFMLGIYTIT LCSLWALWGG V 

« Hide

Isoform 2 [UniParc].

Checksum: 8C69BE27437D6D03
Show »

FASTA43049,285

References

« Hide 'large scale' references
[1]"The pharmacological and functional characteristics of the serotonin 5-HT(3A) receptor are specifically modified by a 5-HT(3B) receptor subunit."
Dubin A.E., Huvar R., D'Andrea M.R., Pyati J., Zhu J.Y., Joy K.C., Wilson S.J., Galindo J.E., Glass C.A., Luo L., Jackson M.R., Lovenberg T.W., Erlander M.G.
J. Biol. Chem. 274:30799-30810(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Small intestine.
[2]"The 5-HT3B subunit is a major determinant of serotonin-receptor function."
Davies P.A., Pistis M., Hanna M.C., Peters J.A., Lambert J.J., Hales T.G., Kirkness E.F.
Nature 397:359-363(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBUNIT.
[3]"Tissue-specific alternative promoters of the serotonin receptor gene HTR3B in human brain and intestine."
Tzvetkov M.V., Meineke C., Oetjen E., Hirsch-Ernst K., Brockmoller J.
Gene 386:52-62(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT SER-129.
Tissue: Brain.
[4]NHLBI resequencing and genotyping service (RS&G)
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"A cytoplasmic region determines single-channel conductance in 5-HT3 receptors."
Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.
Nature 424:321-324(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, REGION.
[7]"Characterization of the novel human serotonin receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E."
Niesler B., Walstab J., Combrink S., Moeller D., Kapeller J., Rietdorf J., Boenisch H., Goethert M., Rappold G., Bruess M.
Mol. Pharmacol. 72:8-17(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[8]"The identification of N-glycosylated residues of the human 5-HT3B receptor subunit: importance for cell membrane expression."
Massoura A.N., Dover T.J., Newman A.S., Barnes N.M.
J. Neurochem. 116:975-983(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION AT ASN-52; ASN-96; ASN-138; ASN-168 AND ASN-203, MUTAGENESIS OF ASN-52; ASN-96; ASN-138; ASN-168 AND ASN-203.
[9]"Investigation of the human serotonin receptor gene HTR3B in bipolar affective and schizophrenic patients."
Frank B., Niesler B., Noethen M.M., Neidt H., Propping P., Bondy B., Rietschel M., Maier W., Albus M., Rappold G.
Am. J. Med. Genet. B Neuropsychiatr. Genet. 131:1-5(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS SER-129; ARG-156 AND ILE-183.
[10]"Mutational analysis of serotonin receptor genes: HTR3A and HTR3B in fibromyalgia patients."
Frank B., Niesler B., Bondy B., Spaeth M., Pongratz D.E., Ackenheil M., Fischer C., Rappold G.
Clin. Rheumatol. 23:338-344(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS SER-129; ARG-156 AND ILE-183.
[11]"Distinguishable haplotype blocks in the HTR3A and HTR3B region in the Japanese reveal evidence of association of HTR3B with female major depression."
Yamada K., Hattori E., Iwayama Y., Ohnishi T., Ohba H., Toyota T., Takao H., Minabe Y., Nakatani N., Higuchi T., Detera-Wadleigh S.D., Yoshikawa T.
Biol. Psychiatry 60:192-201(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS SER-129; THR-143 AND ILE-183.
[12]"A population-specific HTR2B stop codon predisposes to severe impulsivity."
Bevilacqua L., Doly S., Kaprio J., Yuan Q., Tikkanen R., Paunio T., Zhou Z., Wedenoja J., Maroteaux L., Diaz S., Belmer A., Hodgkinson C.A., Dell'osso L., Suvisaari J., Coccaro E., Rose R.J., Peltonen L., Virkkunen M., Goldman D.
Nature 468:1061-1066(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT ARG-156.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF169255 mRNA. Translation: AAF03691.1.
AF080582 mRNA. Translation: AAD12242.1.
AM293589 mRNA. Translation: CAL25321.1.
EF444985 Genomic DNA. Translation: ACA06001.1.
AK314268 mRNA. Translation: BAG36930.1.
RefSeqNP_006019.1. NM_006028.4.
UniGeneHs.241377.

3D structure databases

ProteinModelPortalO95264.
SMRO95264. Positions 30-432.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000260191.

Chemistry

BindingDBO95264.
ChEMBLCHEMBL2094132.

PTM databases

PhosphoSiteO95264.

Proteomic databases

PaxDbO95264.
PRIDEO95264.

Protocols and materials databases

DNASU9177.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000260191; ENSP00000260191; ENSG00000149305. [O95264-1]
ENST00000537778; ENSP00000443118; ENSG00000149305. [O95264-2]
GeneID9177.
KEGGhsa:9177.
UCSCuc001pok.3. human. [O95264-1]
uc001pol.3. human. [O95264-2]

Organism-specific databases

CTD9177.
GeneCardsGC11P113775.
HGNCHGNC:5298. HTR3B.
HPAHPA039559.
MIM604654. gene.
neXtProtNX_O95264.
PharmGKBPA29556.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG260507.
HOGENOMHOG000241519.
HOVERGENHBG106638.
InParanoidO95264.
KOK04819.
OMAEVWNDEF.
OrthoDBEOG7FXZZK.
PhylomeDBO95264.
TreeFamTF315605.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressO95264.
BgeeO95264.
CleanExHS_HTR3B.
GenevestigatorO95264.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR008132. 5HT3_rcpt.
IPR008134. 5HT3_rcpt_B.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR01710. 5HT3BRECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
ProtoNetSearch...

Other

GeneWikiHTR3B.
GenomeRNAi9177.
NextBio34409.
PROO95264.
SOURCESearch...

Entry information

Entry name5HT3B_HUMAN
AccessionPrimary (citable) accession number: O95264
Secondary accession number(s): B0YJ23, Q0VJC3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM