O95263 (PDE8B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
Short name=HsPDE8B
EC=3.1.4.17

Alternative name(s):
Cell proliferation-inducing gene 22 protein

Gene names

Name:	PDE8B
ORF Names:	PIG22

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. Ref.2
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Inhibited by dipyridimole. Insensitive to selective PDE inhibitors including rolipram and milrinone as well as to the non-selective inhibitor, IBMX. Unaffected by cGMP. Ref.2
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Tissue specificity	Abundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates. Ref.1 Ref.2
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Involvement in disease	Striatal degeneration autosomal dominant (ADSD) [MIM:609161]: A movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9 Primary pigmented nodular adrenocortical disease 3 (PPNAD3) [MIM:614190]: A rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. Adrenal glands show overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Disease	Cushing syndrome Disease mutation
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway cyclic nucleotide metabolic process Non-traceable author statement Ref.6. Source: UniProtKB intracellular signal transduction Inferred from electronic annotation. Source: GOC negative regulation of insulin secretion Inferred from electronic annotation. Source: Compara regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity Non-traceable author statement Ref.6. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95263-1)

Also known as: PDE8B1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Major isoform.

Isoform 2 (identifier: O95263-2)

Also known as: PDE8B2; PDE8B3;

The sequence of this isoform differs from the canonical sequence as follows:
     293-389: Missing.

Isoform 3 (identifier: O95263-3)

Also known as: PDE8B3;

The sequence of this isoform differs from the canonical sequence as follows:
     456-510: Missing.

Isoform 4 (identifier: O95263-4)

Also known as: PDE8B4;

The sequence of this isoform differs from the canonical sequence as follows:
     114-133: Missing.

Isoform 5 (identifier: O95263-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-535: Missing.

Note: No experimental confirmation available.

Isoform 6 (identifier: O95263-6)

Also known as: PDE8B2;

The sequence of this isoform differs from the canonical sequence as follows:
     293-339: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B		PRO_0000198840
Regions
	Domain	267 – 338	72	PAS
	Region	590 – 868	279	Catalytic By similarity
Sites
	Active site	615	1	Proton donor By similarity
	Metal binding	619	1	Divalent metal cation 1 By similarity
	Metal binding	655	1	Divalent metal cation 1 By similarity
	Metal binding	656	1	Divalent metal cation 1 By similarity
	Metal binding	656	1	Divalent metal cation 2 By similarity
	Metal binding	781	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	517	1	Phosphoserine Ref.8 Ref.10
Natural variations
	Alternative sequence	1 – 535	535	Missing in isoform 5.		VSP_008081
	Alternative sequence	114 – 133	20	Missing in isoform 4.		VSP_008082
	Alternative sequence	293 – 389	97	Missing in isoform 2.		VSP_008084
	Alternative sequence	293 – 339	47	Missing in isoform 6.		VSP_008083
	Alternative sequence	456 – 510	55	Missing in isoform 3.		VSP_008085
	Natural variant	305	1	H → P in PPNAD3; shows significantly higher cyclic AMP levels after transfection with the mutant protein than after transfection with the wild-type, indicating an impaired ability of the mutant protein to degrade cAMP. Ref.11		VAR_066503
Experimental info
	Sequence conflict	147	1	G → R in CAD38584. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PDE8B1) [UniParc]. Last modified August 22, 2003. Version 2. Checksum: DB4F763E51F745A3	FASTA	885	98,979
	10 20 30 40 50 60 MGCAPSIHVS QSGVIYCRDS DESSSPRQTT SVSQGPAAPL PGLFVQTDAA DAIPPSRASG 70 80 90 100 110 120 PPSVARVRRA RTELGSGSSA GSAAPAATTS RGRRRHCCSS AEAETQTCYT SVKQVSSAEV 130 140 150 160 170 180 RIGPMRLTQD PIQVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII 190 200 210 220 230 240 VIDHRQTQNF DAEAVCRSIR ATNPSEHTVI LAVVSRVSDD HEEASVLPLL HAGFNRRFME 250 260 270 280 290 300 NSSIIACYNE LIQIEHGEVR SQFKLRACNS VFTALDHCHE AIEITSDDHV IQYVNPAFER 310 320 330 340 350 360 MMGYHKGELL GKELADLPKS DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK 370 380 390 400 410 420 ITPVIGQGGK IRHFVSLKKL CCTTDNNKQI HKIHRDSGDN SQTEPHSFRY KNRRKESIDV 430 440 450 460 470 480 KSISSRGSDA PSLQNRRYPS MARIHSMTIE APITKVINII NAAQENSPVT VAEALDRVLE 490 500 510 520 530 540 ILRTTELYSP QLGTKDEDPH TSDLVGGLMT DGLRRLSGNE YVFTKNVHQS HSHLAMPITI 550 560 570 580 590 600 NDVPPCISQL LDNEESWDFN IFELEAITHK RPLVYLGLKV FSRFGVCEFL NCSETTLRAW 610 620 630 640 650 660 FQVIEANYHS SNAYHNSTHA ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP 670 680 690 700 710 720 GRTNSFLCNA GSELAVLYND TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI 730 740 750 760 770 780 IDMVLATEMT KHFEHVNKFV NSINKPMAAE IEGSDCECNP AGKNFPENQI LIKRMMIKCA 790 800 810 820 830 840 DVANPCRPLD LCIEWAGRIS EEYFAQTDEE KRQGLPVVMP VFDRNTCSIP KSQISFIDYF 850 860 870 880 ITDMFDAWDA FAHLPALMQH LADNYKHWKT LDDLKCKSLR LPSDS
	Isoform 2 (PDE8B2) (PDE8B3) [UniParc]. Checksum: 816AECACCAE45447	FASTA	788	87,974
	10 20 30 40 50 60 MGCAPSIHVS QSGVIYCRDS DESSSPRQTT SVSQGPAAPL PGLFVQTDAA DAIPPSRASG 70 80 90 100 110 120 PPSVARVRRA RTELGSGSSA GSAAPAATTS RGRRRHCCSS AEAETQTCYT SVKQVSSAEV 130 140 150 160 170 180 RIGPMRLTQD PIQVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII 190 200 210 220 230 240 VIDHRQTQNF DAEAVCRSIR ATNPSEHTVI LAVVSRVSDD HEEASVLPLL HAGFNRRFME 250 260 270 280 290 300 NSSIIACYNE LIQIEHGEVR SQFKLRACNS VFTALDHCHE AIEITSDDHV IQIHKIHRDS 310 320 330 340 350 360 GDNSQTEPHS FRYKNRRKES IDVKSISSRG SDAPSLQNRR YPSMARIHSM TIEAPITKVI 370 380 390 400 410 420 NIINAAQENS PVTVAEALDR VLEILRTTEL YSPQLGTKDE DPHTSDLVGG LMTDGLRRLS 430 440 450 460 470 480 GNEYVFTKNV HQSHSHLAMP ITINDVPPCI SQLLDNEESW DFNIFELEAI THKRPLVYLG 490 500 510 520 530 540 LKVFSRFGVC EFLNCSETTL RAWFQVIEAN YHSSNAYHNS THAADVLHAT AFFLGKERVK 550 560 570 580 590 600 GSLDQLDEVA ALIAATVHDV DHPGRTNSFL CNAGSELAVL YNDTAVLESH HTALAFQLTV 610 620 630 640 650 660 KDTKCNIFKN IDRNHYRTLR QAIIDMVLAT EMTKHFEHVN KFVNSINKPM AAEIEGSDCE 670 680 690 700 710 720 CNPAGKNFPE NQILIKRMMI KCADVANPCR PLDLCIEWAG RISEEYFAQT DEEKRQGLPV 730 740 750 760 770 780 VMPVFDRNTC SIPKSQISFI DYFITDMFDA WDAFAHLPAL MQHLADNYKH WKTLDDLKCK SLRLPSDS
	Isoform 3 (PDE8B3) [UniParc]. Checksum: 5A39ED08E2A877E4	FASTA	830	93,047
	10 20 30 40 50 60 MGCAPSIHVS QSGVIYCRDS DESSSPRQTT SVSQGPAAPL PGLFVQTDAA DAIPPSRASG 70 80 90 100 110 120 PPSVARVRRA RTELGSGSSA GSAAPAATTS RGRRRHCCSS AEAETQTCYT SVKQVSSAEV 130 140 150 160 170 180 RIGPMRLTQD PIQVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII 190 200 210 220 230 240 VIDHRQTQNF DAEAVCRSIR ATNPSEHTVI LAVVSRVSDD HEEASVLPLL HAGFNRRFME 250 260 270 280 290 300 NSSIIACYNE LIQIEHGEVR SQFKLRACNS VFTALDHCHE AIEITSDDHV IQYVNPAFER 310 320 330 340 350 360 MMGYHKGELL GKELADLPKS DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK 370 380 390 400 410 420 ITPVIGQGGK IRHFVSLKKL CCTTDNNKQI HKIHRDSGDN SQTEPHSFRY KNRRKESIDV 430 440 450 460 470 480 KSISSRGSDA PSLQNRRYPS MARIHSMTIE APITKDGLRR LSGNEYVFTK NVHQSHSHLA 490 500 510 520 530 540 MPITINDVPP CISQLLDNEE SWDFNIFELE AITHKRPLVY LGLKVFSRFG VCEFLNCSET 550 560 570 580 590 600 TLRAWFQVIE ANYHSSNAYH NSTHAADVLH ATAFFLGKER VKGSLDQLDE VAALIAATVH 610 620 630 640 650 660 DVDHPGRTNS FLCNAGSELA VLYNDTAVLE SHHTALAFQL TVKDTKCNIF KNIDRNHYRT 670 680 690 700 710 720 LRQAIIDMVL ATEMTKHFEH VNKFVNSINK PMAAEIEGSD CECNPAGKNF PENQILIKRM 730 740 750 760 770 780 MIKCADVANP CRPLDLCIEW AGRISEEYFA QTDEEKRQGL PVVMPVFDRN TCSIPKSQIS 790 800 810 820 830 FIDYFITDMF DAWDAFAHLP ALMQHLADNY KHWKTLDDLK CKSLRLPSDS
	Isoform 4 (PDE8B4) [UniParc]. Checksum: DDC83E5525C7E7B7	FASTA	865	96,771
	10 20 30 40 50 60 MGCAPSIHVS QSGVIYCRDS DESSSPRQTT SVSQGPAAPL PGLFVQTDAA DAIPPSRASG 70 80 90 100 110 120 PPSVARVRRA RTELGSGSSA GSAAPAATTS RGRRRHCCSS AEAETQTCYT SVKVLLIFAK 130 140 150 160 170 180 EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII VIDHRQTQNF DAEAVCRSIR 190 200 210 220 230 240 ATNPSEHTVI LAVVSRVSDD HEEASVLPLL HAGFNRRFME NSSIIACYNE LIQIEHGEVR 250 260 270 280 290 300 SQFKLRACNS VFTALDHCHE AIEITSDDHV IQYVNPAFER MMGYHKGELL GKELADLPKS 310 320 330 340 350 360 DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK ITPVIGQGGK IRHFVSLKKL 370 380 390 400 410 420 CCTTDNNKQI HKIHRDSGDN SQTEPHSFRY KNRRKESIDV KSISSRGSDA PSLQNRRYPS 430 440 450 460 470 480 MARIHSMTIE APITKVINII NAAQENSPVT VAEALDRVLE ILRTTELYSP QLGTKDEDPH 490 500 510 520 530 540 TSDLVGGLMT DGLRRLSGNE YVFTKNVHQS HSHLAMPITI NDVPPCISQL LDNEESWDFN 550 560 570 580 590 600 IFELEAITHK RPLVYLGLKV FSRFGVCEFL NCSETTLRAW FQVIEANYHS SNAYHNSTHA 610 620 630 640 650 660 ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP GRTNSFLCNA GSELAVLYND 670 680 690 700 710 720 TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI IDMVLATEMT KHFEHVNKFV 730 740 750 760 770 780 NSINKPMAAE IEGSDCECNP AGKNFPENQI LIKRMMIKCA DVANPCRPLD LCIEWAGRIS 790 800 810 820 830 840 EEYFAQTDEE KRQGLPVVMP VFDRNTCSIP KSQISFIDYF ITDMFDAWDA FAHLPALMQH 850 860 LADNYKHWKT LDDLKCKSLR LPSDS
	Isoform 5 [UniParc]. Checksum: A6D4F19BE26C045F	FASTA	350	39,791
	10 20 30 40 50 60 MPITINDVPP CISQLLDNEE SWDFNIFELE AITHKRPLVY LGLKVFSRFG VCEFLNCSET 70 80 90 100 110 120 TLRAWFQVIE ANYHSSNAYH NSTHAADVLH ATAFFLGKER VKGSLDQLDE VAALIAATVH 130 140 150 160 170 180 DVDHPGRTNS FLCNAGSELA VLYNDTAVLE SHHTALAFQL TVKDTKCNIF KNIDRNHYRT 190 200 210 220 230 240 LRQAIIDMVL ATEMTKHFEH VNKFVNSINK PMAAEIEGSD CECNPAGKNF PENQILIKRM 250 260 270 280 290 300 MIKCADVANP CRPLDLCIEW AGRISEEYFA QTDEEKRQGL PVVMPVFDRN TCSIPKSQIS 310 320 330 340 350 FIDYFITDMF DAWDAFAHLP ALMQHLADNY KHWKTLDDLK CKSLRLPSDS
	Isoform 6 (PDE8B2) [UniParc]. Checksum: 013C790AC0F24F92	FASTA	838	93,674
	10 20 30 40 50 60 MGCAPSIHVS QSGVIYCRDS DESSSPRQTT SVSQGPAAPL PGLFVQTDAA DAIPPSRASG 70 80 90 100 110 120 PPSVARVRRA RTELGSGSSA GSAAPAATTS RGRRRHCCSS AEAETQTCYT SVKQVSSAEV 130 140 150 160 170 180 RIGPMRLTQD PIQVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII 190 200 210 220 230 240 VIDHRQTQNF DAEAVCRSIR ATNPSEHTVI LAVVSRVSDD HEEASVLPLL HAGFNRRFME 250 260 270 280 290 300 NSSIIACYNE LIQIEHGEVR SQFKLRACNS VFTALDHCHE AIEITSDDHV IQEWQGVYYA 310 320 330 340 350 360 RRKSGDSIQQ HVKITPVIGQ GGKIRHFVSL KKLCCTTDNN KQIHKIHRDS GDNSQTEPHS 370 380 390 400 410 420 FRYKNRRKES IDVKSISSRG SDAPSLQNRR YPSMARIHSM TIEAPITKVI NIINAAQENS 430 440 450 460 470 480 PVTVAEALDR VLEILRTTEL YSPQLGTKDE DPHTSDLVGG LMTDGLRRLS GNEYVFTKNV 490 500 510 520 530 540 HQSHSHLAMP ITINDVPPCI SQLLDNEESW DFNIFELEAI THKRPLVYLG LKVFSRFGVC 550 560 570 580 590 600 EFLNCSETTL RAWFQVIEAN YHSSNAYHNS THAADVLHAT AFFLGKERVK GSLDQLDEVA 610 620 630 640 650 660 ALIAATVHDV DHPGRTNSFL CNAGSELAVL YNDTAVLESH HTALAFQLTV KDTKCNIFKN 670 680 690 700 710 720 IDRNHYRTLR QAIIDMVLAT EMTKHFEHVN KFVNSINKPM AAEIEGSDCE CNPAGKNFPE 730 740 750 760 770 780 NQILIKRMMI KCADVANPCR PLDLCIEWAG RISEEYFAQT DEEKRQGLPV VMPVFDRNTC 790 800 810 820 830 SIPKSQISFI DYFITDMFDA WDAFAHLPAL MQHLADNYKH WKTLDDLKCK SLRLPSDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization, chromosomal localization, and alternative splicing of the human phosphodiesterase 8B gene." Hayashi M., Shimada Y., Nishimura Y., Hama T., Tanaka T. Biochem. Biophys. Res. Commun. 297:1253-1258(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2 AND 6), TISSUE SPECIFICITY.
[2]	"Comparison of enzymatic characterization and gene organization of cyclic nucleotide phosphodiesterase 8 family in humans." Gamanuma M., Yuasa K., Sasaki T., Sakurai N., Kotera J., Omori K. Cell. Signal. 15:565-574(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY. Tissue: Thyroid.
[3]	"Identification of a human proliferation-inducing gene." Kim J.W. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Testis.
[6]	"Molecular cloning and characterization of human PDE8B, a novel thyroid-specific isozyme of 3',5'-cyclic nucleotide phosphodiesterase." Hayashi M., Matsushima K., Ohashi H., Tsunoda H., Murase S., Kawarada Y., Tanaka T. Biochem. Biophys. Res. Commun. 250:751-756(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-885 (ISOFORM 1).
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 43-885 (ISOFORM 1). Tissue: Amygdala.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Autosomal-dominant striatal degeneration is caused by a mutation in the phosphodiesterase 8B gene." Appenzeller S., Schirmacher A., Halfter H., Baumer S., Pendziwiat M., Timmerman V., De Jonghe P., Fekete K., Stogbauer F., Ludemann P., Hund M., Quabius E.S., Ringelstein E.B., Kuhlenbaumer G. Am. J. Hum. Genet. 86:83-87(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN ADSD.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex." Horvath A., Giatzakis C., Tsang K., Greene E., Osorio P., Boikos S., Libe R., Patronas Y., Robinson-White A., Remmers E., Bertherat J., Nesterova M., Stratakis C.A. Eur. J. Hum. Genet. 16:1245-1253(2008) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT PPNAD3 PRO-305, CHARACTERIZATION OF VARIANT PPNAD3 PRO-305.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY129948 mRNA. Translation: AAN71723.1. AY129949 mRNA. Translation: AAN71724.1. AY129950 Genomic DNA. Translation: AAN71725.1. AY129950 Genomic DNA. Translation: AAN71726.1. AY129950 Genomic DNA. Translation: AAN71727.1. AB085824 mRNA. Translation: BAC53762.1. AB085825 mRNA. Translation: BAC53763.1. AB085826 mRNA. Translation: BAC53764.1. AB085827 mRNA. Translation: BAC53765.1. AY423729 mRNA. Translation: AAS00492.1. CH471084 Genomic DNA. Translation: EAW95803.1. BC043209 mRNA. No translation available. AF079529 mRNA. Translation: AAC69564.2. AL831924 mRNA. Translation: CAD38584.1.
IPI	IPI00216767. IPI00216768. IPI00294049. IPI00337505. IPI00337506. IPI00337507.
PIR	JE0293.
RefSeq	NP_001025022.1. NM_001029851.2. NP_001025023.1. NM_001029852.2. NP_001025024.1. NM_001029853.2. NP_001025025.1. NM_001029854.2. NP_003710.1. NM_003719.3.
UniGene	Hs.584830.
3D structure databases
ProteinModelPortal	O95263.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000264917.
PTM databases
PhosphoSite	O95263.
2D gel databases
UCD-2DPAGE	O95263.
Proteomic databases
PaxDb	O95263.
PRIDE	O95263.
Protocols and materials databases
DNASU	8622.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264917; ENSP00000264917; ENSG00000113231. ENST00000333194; ENSP00000331336; ENSG00000113231. ENST00000340978; ENSP00000345446; ENSG00000113231. ENST00000342343; ENSP00000345646; ENSG00000113231. ENST00000346042; ENSP00000330428; ENSG00000113231. ENST00000505283; ENSP00000423461; ENSG00000113231.
GeneID	8622.
KEGG	hsa:8622.
UCSC	uc003kfa.3. human. uc003kfb.3. human. uc003kfc.3. human. uc003kfd.3. human. uc003kfe.3. human.
Organism-specific databases
CTD	8622.
GeneCards	GC05P076542.
H-InvDB	HIX0004971.
HGNC	HGNC:8794. PDE8B.
HPA	HPA036912.
MIM	603390. gene. 609161. phenotype. 614190. phenotype.
neXtProt	NX_O95263.
Orphanet	228169. Autosomal dominant striatal neurodegeneration. 189439. Primary pigmented nodular adrenocortical disease.
PharmGKB	PA33142.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG282089.
HOVERGEN	HBG053544.
InParanoid	O95263.
KO	K01120.
OMA	LCCTTDN.
PhylomeDB	O95263.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
UniPathway	UPA00762; UER00747.
Gene expression databases
ArrayExpress	O95263.
Bgee	O95263.
CleanEx	HS_PDE8B.
Genevestigator	O95263.
GermOnline	ENSG00000113231. Homo sapiens.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR000014. PAS. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. IPR013938. PDEase_PDE8. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF08629. PDE8. 1 hit. PF00233. PDEase_I. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50112. PAS. 1 hit. PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O95263.
ChEMBL	CHEMBL4408.
GenomeRNAi	8622.
NextBio	32309.
SOURCE	Search...

Entry information

Entry name

PDE8B_HUMAN

Accession

Primary (citable) accession number: O95263
Secondary accession number(s): Q5J7V7 , Q86XK8, Q8IUJ7, Q8IUJ8, Q8IUJ9, Q8IUK0, Q8N3T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 22, 2003
Last modified:	May 1, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.