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O95263 (PDE8B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Short name=HsPDE8B
EC=3.1.4.53
Alternative name(s):
Cell proliferation-inducing gene 22 protein
Gene names
Name:PDE8B
ORF Names:PIG22
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length885 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. Ref.2

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulation

Inhibited by dipyridimole. Insensitive to selective PDE inhibitors including rolipram and milrinone as well as to the non-selective inhibitor, IBMX. Unaffected by cGMP. Ref.2

Pathway

Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Tissue specificity

Abundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates. Ref.1 Ref.2

Domain

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Involvement in disease

Striatal degeneration autosomal dominant (ADSD) [MIM:609161]: A movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9

Primary pigmented nodular adrenocortical disease 3 (PPNAD3) [MIM:614190]: A rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. Adrenal glands show overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. Clinical manifestations of Cushing syndrome include facial and truncal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.

Contains 1 PAS (PER-ARNT-SIM) domain.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95263-1)

Also known as: PDE8B1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Major isoform.
Isoform 2 (identifier: O95263-2)

Also known as: PDE8B2; PDE8B3;

The sequence of this isoform differs from the canonical sequence as follows:
     293-389: Missing.
Isoform 3 (identifier: O95263-3)

Also known as: PDE8B3;

The sequence of this isoform differs from the canonical sequence as follows:
     456-510: Missing.
Isoform 4 (identifier: O95263-4)

Also known as: PDE8B4;

The sequence of this isoform differs from the canonical sequence as follows:
     114-133: Missing.
Isoform 5 (identifier: O95263-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-535: Missing.
Note: No experimental confirmation available.
Isoform 6 (identifier: O95263-6)

Also known as: PDE8B2;

The sequence of this isoform differs from the canonical sequence as follows:
     293-339: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 885885High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
PRO_0000198840

Regions

Domain267 – 33872PAS
Region590 – 868279Catalytic By similarity

Sites

Active site6151Proton donor By similarity
Metal binding6191Divalent metal cation 1 By similarity
Metal binding6551Divalent metal cation 1 By similarity
Metal binding6561Divalent metal cation 1 By similarity
Metal binding6561Divalent metal cation 2 By similarity
Metal binding7811Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue5171Phosphoserine Ref.8 Ref.10

Natural variations

Alternative sequence1 – 535535Missing in isoform 5.
VSP_008081
Alternative sequence114 – 13320Missing in isoform 4.
VSP_008082
Alternative sequence293 – 38997Missing in isoform 2.
VSP_008084
Alternative sequence293 – 33947Missing in isoform 6.
VSP_008083
Alternative sequence456 – 51055Missing in isoform 3.
VSP_008085
Natural variant3051H → P in PPNAD3; shows significantly higher cyclic AMP levels after transfection with the mutant protein than after transfection with the wild-type, indicating an impaired ability of the mutant protein to degrade cAMP. Ref.11
VAR_066503

Experimental info

Sequence conflict1471G → R in CAD38584. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PDE8B1) [UniParc].

Last modified August 22, 2003. Version 2.
Checksum: DB4F763E51F745A3

FASTA88598,979
        10         20         30         40         50         60 
MGCAPSIHVS QSGVIYCRDS DESSSPRQTT SVSQGPAAPL PGLFVQTDAA DAIPPSRASG 

        70         80         90        100        110        120 
PPSVARVRRA RTELGSGSSA GSAAPAATTS RGRRRHCCSS AEAETQTCYT SVKQVSSAEV 

       130        140        150        160        170        180 
RIGPMRLTQD PIQVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII 

       190        200        210        220        230        240 
VIDHRQTQNF DAEAVCRSIR ATNPSEHTVI LAVVSRVSDD HEEASVLPLL HAGFNRRFME 

       250        260        270        280        290        300 
NSSIIACYNE LIQIEHGEVR SQFKLRACNS VFTALDHCHE AIEITSDDHV IQYVNPAFER 

       310        320        330        340        350        360 
MMGYHKGELL GKELADLPKS DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK 

       370        380        390        400        410        420 
ITPVIGQGGK IRHFVSLKKL CCTTDNNKQI HKIHRDSGDN SQTEPHSFRY KNRRKESIDV 

       430        440        450        460        470        480 
KSISSRGSDA PSLQNRRYPS MARIHSMTIE APITKVINII NAAQENSPVT VAEALDRVLE 

       490        500        510        520        530        540 
ILRTTELYSP QLGTKDEDPH TSDLVGGLMT DGLRRLSGNE YVFTKNVHQS HSHLAMPITI 

       550        560        570        580        590        600 
NDVPPCISQL LDNEESWDFN IFELEAITHK RPLVYLGLKV FSRFGVCEFL NCSETTLRAW 

       610        620        630        640        650        660 
FQVIEANYHS SNAYHNSTHA ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP 

       670        680        690        700        710        720 
GRTNSFLCNA GSELAVLYND TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI 

       730        740        750        760        770        780 
IDMVLATEMT KHFEHVNKFV NSINKPMAAE IEGSDCECNP AGKNFPENQI LIKRMMIKCA 

       790        800        810        820        830        840 
DVANPCRPLD LCIEWAGRIS EEYFAQTDEE KRQGLPVVMP VFDRNTCSIP KSQISFIDYF 

       850        860        870        880 
ITDMFDAWDA FAHLPALMQH LADNYKHWKT LDDLKCKSLR LPSDS 

« Hide

Isoform 2 (PDE8B2) (PDE8B3) [UniParc].

Checksum: 816AECACCAE45447
Show »

FASTA78887,974
Isoform 3 (PDE8B3) [UniParc].

Checksum: 5A39ED08E2A877E4
Show »

FASTA83093,047
Isoform 4 (PDE8B4) [UniParc].

Checksum: DDC83E5525C7E7B7
Show »

FASTA86596,771
Isoform 5 [UniParc].

Checksum: A6D4F19BE26C045F
Show »

FASTA35039,791
Isoform 6 (PDE8B2) [UniParc].

Checksum: 013C790AC0F24F92
Show »

FASTA83893,674

References

« Hide 'large scale' references
[1]"Genomic organization, chromosomal localization, and alternative splicing of the human phosphodiesterase 8B gene."
Hayashi M., Shimada Y., Nishimura Y., Hama T., Tanaka T.
Biochem. Biophys. Res. Commun. 297:1253-1258(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2 AND 6), TISSUE SPECIFICITY.
[2]"Comparison of enzymatic characterization and gene organization of cyclic nucleotide phosphodiesterase 8 family in humans."
Gamanuma M., Yuasa K., Sasaki T., Sakurai N., Kotera J., Omori K.
Cell. Signal. 15:565-574(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY.
Tissue: Thyroid.
[3]"Identification of a human proliferation-inducing gene."
Kim J.W.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
Tissue: Testis.
[6]"Molecular cloning and characterization of human PDE8B, a novel thyroid-specific isozyme of 3',5'-cyclic nucleotide phosphodiesterase."
Hayashi M., Matsushima K., Ohashi H., Tsunoda H., Murase S., Kawarada Y., Tanaka T.
Biochem. Biophys. Res. Commun. 250:751-756(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-885 (ISOFORM 1).
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 43-885 (ISOFORM 1).
Tissue: Amygdala.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Autosomal-dominant striatal degeneration is caused by a mutation in the phosphodiesterase 8B gene."
Appenzeller S., Schirmacher A., Halfter H., Baumer S., Pendziwiat M., Timmerman V., De Jonghe P., Fekete K., Stogbauer F., Ludemann P., Hund M., Quabius E.S., Ringelstein E.B., Kuhlenbaumer G.
Am. J. Hum. Genet. 86:83-87(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN ADSD.
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex."
Horvath A., Giatzakis C., Tsang K., Greene E., Osorio P., Boikos S., Libe R., Patronas Y., Robinson-White A., Remmers E., Bertherat J., Nesterova M., Stratakis C.A.
Eur. J. Hum. Genet. 16:1245-1253(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT PPNAD3 PRO-305, CHARACTERIZATION OF VARIANT PPNAD3 PRO-305.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY129948 mRNA. Translation: AAN71723.1.
AY129949 mRNA. Translation: AAN71724.1.
AY129950 Genomic DNA. Translation: AAN71725.1.
AY129950 Genomic DNA. Translation: AAN71726.1.
AY129950 Genomic DNA. Translation: AAN71727.1.
AB085824 mRNA. Translation: BAC53762.1.
AB085825 mRNA. Translation: BAC53763.1.
AB085826 mRNA. Translation: BAC53764.1.
AB085827 mRNA. Translation: BAC53765.1.
AY423729 mRNA. Translation: AAS00492.1.
CH471084 Genomic DNA. Translation: EAW95803.1.
BC043209 mRNA. No translation available.
AF079529 mRNA. Translation: AAC69564.2.
AL831924 mRNA. Translation: CAD38584.1.
PIRJE0293.
RefSeqNP_001025022.1. NM_001029851.2.
NP_001025023.1. NM_001029852.2.
NP_001025024.1. NM_001029853.2.
NP_001025025.1. NM_001029854.2.
NP_003710.1. NM_003719.3.
UniGeneHs.584830.

3D structure databases

ProteinModelPortalO95263.
SMRO95263. Positions 274-383, 541-874.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114177. 1 interaction.
STRING9606.ENSP00000264917.

Chemistry

BindingDBO95263.
ChEMBLCHEMBL4408.
GuidetoPHARMACOLOGY1308.

PTM databases

PhosphoSiteO95263.

2D gel databases

UCD-2DPAGEO95263.

Proteomic databases

PaxDbO95263.
PRIDEO95263.

Protocols and materials databases

DNASU8622.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264917; ENSP00000264917; ENSG00000113231. [O95263-1]
ENST00000333194; ENSP00000331336; ENSG00000113231. [O95263-3]
ENST00000340978; ENSP00000345446; ENSG00000113231. [O95263-6]
ENST00000342343; ENSP00000345646; ENSG00000113231. [O95263-4]
ENST00000346042; ENSP00000330428; ENSG00000113231. [O95263-2]
ENST00000505283; ENSP00000423461; ENSG00000113231. [O95263-5]
GeneID8622.
KEGGhsa:8622.
UCSCuc003kfa.3. human. [O95263-1]
uc003kfb.3. human. [O95263-4]
uc003kfc.3. human. [O95263-3]
uc003kfd.3. human. [O95263-6]
uc003kfe.3. human. [O95263-2]

Organism-specific databases

CTD8622.
GeneCardsGC05P076542.
H-InvDBHIX0004971.
HGNCHGNC:8794. PDE8B.
HPAHPA036912.
MIM603390. gene.
609161. phenotype.
614190. phenotype.
neXtProtNX_O95263.
Orphanet228169. Autosomal dominant striatal neurodegeneration.
189439. Primary pigmented nodular adrenocortical disease.
PharmGKBPA33142.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG282089.
HOVERGENHBG053544.
InParanoidO95263.
KOK01120.
OMADHRQTQN.
OrthoDBEOG7X3QQM.
PhylomeDBO95263.
TreeFamTF314638.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
UniPathwayUPA00762; UER00747.

Gene expression databases

ArrayExpressO95263.
BgeeO95263.
CleanExHS_PDE8B.
GenevestigatorO95263.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013938. PDEase_PDE8.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamPF13426. PAS_9. 1 hit.
PF08629. PDE8. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPDE8B.
GenomeRNAi8622.
NextBio32309.
PROO95263.
SOURCESearch...

Entry information

Entry namePDE8B_HUMAN
AccessionPrimary (citable) accession number: O95263
Secondary accession number(s): Q5J7V7 expand/collapse secondary AC list , Q86XK8, Q8IUJ7, Q8IUJ8, Q8IUJ9, Q8IUK0, Q8N3T2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 22, 2003
Last modified: April 16, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM