Reviewed, UniProtKB/Swiss-Prot O95260 (ATE1_HUMAN)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA--protein transferase 1
      Short name=Arginyltransferase 1
      Short name=R-transferase 1
    EC=2.3.2.8
Alternative name(s):
    Arginine-tRNA--protein transferase 1

Gene names

Name:

ATE1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues By similarity.
Catalytic activity	L-arginyl-tRNA + protein = tRNA + L-arginyl-protein.
Subunit structure	Monomer Potential.
Subcellular location	Isoform ATE1-1: Nucleus By similarity. Cytoplasm By similarity. Isoform ATE1-2: Cytoplasm By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.5
Sequence similarities	Belongs to the R-transferase family.
Caution	It is uncertain whether Met-1 or Met-37 is the initiator.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein arginylation Ref.4 Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: UniProtKB nucleus Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW arginyltransferase activity Ref.4 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform ATE1-1 (identifier: O95260-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform ATE1-2 (identifier: O95260-2)

The sequence of this isoform differs from the canonical sequence as follows:
     274-314: VVRSSPPSSQ...NPPDTPTESQ → LVPVSFEDPE...DPPDECGKTE

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Arginyl-tRNA--protein transferase 1		PRO_0000195088
Amino acid modifications
	Modified residue	169	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	274 – 314	41	VVRSS…PTESQ → LVPVSFEDPEFKSSFSQSFS LYVKYQVAIHQDPPDECGKT E in isoform ATE1-2.		VSP_000336
Experimental info
	Sequence conflict	5 – 11	7	AGGSPSV → GGGFAAS in AAD12366. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform ATE1-1 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: BFD1CF8925CF5820	FASTA	518	59,090
	10 20 30 40 50 60 MAFWAGGSPS VVDYFPSEDF YRCGYCKNES GSRSNGMWAH SMTVQDYQDL IDRGWRRSGK 70 80 90 100 110 120 YVYKPVMNQT CCPQYTIRCR PLQFQPSKSH KKVLKKMLKF LAKGEVPKGS CEDEPMDSTM 130 140 150 160 170 180 DDAVAGDFAL INKLDIQCDL KTLSDDIKES LESEGKNSKK EEPQELLQSQ DFVGEKLGSG 190 200 210 220 230 240 EPSHSVKVHT VPKPGKGADL SKPPCRKAKE IRKERKRLKL MQQNPAGELE GFQAQGHPPS 250 260 270 280 290 300 LFPPKAKSNQ PKSLEDLIFE SLPENASHKL EVRVVRSSPP SSQFKATLLE SYQVYKRYQM 310 320 330 340 350 360 VIHKNPPDTP TESQFTRFLC SSPLEAETPP NGPDCGYGSF HQQYWLDGKI IAVGVIDILP 370 380 390 400 410 420 NCVSSVYLYY DPDYSFLSLG VYSALREIAF TRQLHEKTSQ LSYYYMGFYI HSCPKMKYKG 430 440 450 460 470 480 QYRPSDLLCP ETYVWVPIEQ CLPSLENSKY CRFNQDPEAV DEDRSTEPDR LQVFHKRAIM 490 500 510 PYGVYKKQQK DPSEEAAVLQ YASLVGQKCS ERMLLFRN
	Isoform ATE1-2. Checksum: D01E1D6024F04565	FASTA	518	59,032
	10 20 30 40 50 60 MAFWAGGSPS VVDYFPSEDF YRCGYCKNES GSRSNGMWAH SMTVQDYQDL IDRGWRRSGK 70 80 90 100 110 120 YVYKPVMNQT CCPQYTIRCR PLQFQPSKSH KKVLKKMLKF LAKGEVPKGS CEDEPMDSTM 130 140 150 160 170 180 DDAVAGDFAL INKLDIQCDL KTLSDDIKES LESEGKNSKK EEPQELLQSQ DFVGEKLGSG 190 200 210 220 230 240 EPSHSVKVHT VPKPGKGADL SKPPCRKAKE IRKERKRLKL MQQNPAGELE GFQAQGHPPS 250 260 270 280 290 300 LFPPKAKSNQ PKSLEDLIFE SLPENASHKL EVRLVPVSFE DPEFKSSFSQ SFSLYVKYQV 310 320 330 340 350 360 AIHQDPPDEC GKTEFTRFLC SSPLEAETPP NGPDCGYGSF HQQYWLDGKI IAVGVIDILP 370 380 390 400 410 420 NCVSSVYLYY DPDYSFLSLG VYSALREIAF TRQLHEKTSQ LSYYYMGFYI HSCPKMKYKG 430 440 450 460 470 480 QYRPSDLLCP ETYVWVPIEQ CLPSLENSKY CRFNQDPEAV DEDRSTEPDR LQVFHKRAIM 490 500 510 PYGVYKKQQK DPSEEAAVLQ YASLVGQKCS ERMLLFRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ATE1-2). Tissue: Testis.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Alternative splicing results in differential expression, activity, and localization of the two forms of arginyl-tRNA-protein transferase, a component of the N-end rule pathway." Kwon Y.T., Kashina A.S., Varshavsky A. Mol. Cell. Biol. 19:182-193(1999) [PubMed: 9858543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-518 (ISOFORMS ATE1-1 AND ATE1-2). Tissue: Embryonic kidney.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC022026 mRNA. Translation: AAH22026.2. AL731542, AC025947, AL731566 Genomic DNA. Translation: CAI12918.1. AL731566, AC025947, AL731542 Genomic DNA. Translation: CAI14813.1. CH471066 Genomic DNA. Translation: EAW49334.1. AF079098 mRNA. Translation: AAD12366.1. AF079099 mRNA. Translation: AAD12367.1.
IPI	IPI00029472. IPI00218377.
RefSeq	NP_001001976.1. NP_008972.2.
UniGene	Hs.632080
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O95260. 1 interaction.
STRING	O95260.
PTM databases
PhosphoSite	O95260.
Proteomic databases
PRIDE	O95260.
Genome annotation databases
Ensembl	ENST00000224652; ENSP00000224652; ENSG00000107669; Homo sapiens. [Genome view] ENST00000369040; ENSP00000358036; ENSG00000107669; Homo sapiens. [Genome view] ENST00000369043; ENSP00000358039; ENSG00000107669; Homo sapiens. [Genome view] ENST00000423243; ENSP00000397787; ENSG00000107669; Homo sapiens. [Genome view] ENST00000455628; ENSP00000405099; ENSG00000107669; Homo sapiens. [Genome view]
GeneID	11101.
KEGG	hsa:11101.
UCSC	uc001lfp.1. human. uc001lfq.1. human.
Organism-specific databases
CTD	11101.
GeneCards	GC10M123492.
H-InvDB	HIX0009267.
HGNC	HGNC:782. ATE1.
MIM	607103. gene.
PharmGKB	PA25082.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O95260.
HOVERGEN	O95260.
OMA	SIVEYFE.
Enzyme and pathway databases
BRENDA	2.3.2.8. 247.
Gene expression databases
ArrayExpress	O95260.
Bgee	O95260.
CleanEx	HS_ATE1.
Genevestigator	O95260.
GermOnline	ENSG00000107669. Homo sapiens.
Family and domain databases
InterPro	IPR017137. Arg-tRNA-P_Trfase_1_euk. IPR007472. Arg-tRNA-P_Trfase_C. IPR007471. Arg_tRNA_PTrfase_N. [Graphical view]
Pfam	PF04377. ATE_C. 1 hit. PF04376. ATE_N. 1 hit. [Graphical view]
PIRSF	PIRSF037207. ATE1_euk. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	42204.
SOURCE	Search...

Entry information

Entry name

ATE1_HUMAN

Accession

Primary (citable) accession number: O95260
Secondary accession number(s): O95261, Q5SQQ3, Q8WW04

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 26, 2002
Last modified:	November 3, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.