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O95257

- GA45G_HUMAN

UniProt

O95257 - GA45G_HUMAN

Protein

Growth arrest and DNA damage-inducible protein GADD45 gamma

Gene

GADD45G

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. activation of MAPKK activity Source: Ensembl
    2. activation of MAPKKK activity Source: UniProtKB
    3. apoptotic process Source: UniProtKB-KW
    4. cell differentiation Source: UniProtKB-KW
    5. multicellular organismal development Source: UniProtKB-KW
    6. negative regulation of protein kinase activity Source: Ensembl
    7. positive regulation of apoptotic process Source: UniProtKB
    8. positive regulation of JNK cascade Source: UniProtKB
    9. positive regulation of p38MAPK cascade Source: UniProtKB
    10. regulation of cell cycle Source: Ensembl
    11. response to stress Source: InterPro

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Apoptosis, Differentiation

    Enzyme and pathway databases

    SignaLinkiO95257.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Growth arrest and DNA damage-inducible protein GADD45 gamma
    Alternative name(s):
    Cytokine-responsive protein CR6
    DNA damage-inducible transcript 2 protein
    Short name:
    DDIT-2
    Gene namesi
    Name:GADD45G
    Synonyms:CR6, DDIT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:4097. GADD45G.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi47 – 471A → R: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with E-77, E-80 and R-83. 1 Publication
    Mutagenesisi76 – 761I → E: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-80 and R-83. 1 Publication
    Mutagenesisi80 – 801L → E: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-77 and R-83. 1 Publication
    Mutagenesisi83 – 831A → R: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-77 and E-80. 1 Publication
    Mutagenesisi87 – 871E → K: Reduced growth inhibition activity; when associated with K-89. 1 Publication
    Mutagenesisi89 – 891D → K: Reduced growth inhibition activity; when associated with K-87. 1 Publication

    Organism-specific databases

    PharmGKBiPA28512.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 159159Growth arrest and DNA damage-inducible protein GADD45 gammaPRO_0000148336Add
    BLAST

    Proteomic databases

    PaxDbiO95257.
    PRIDEiO95257.

    Expressioni

    Gene expression databases

    ArrayExpressiO95257.
    BgeeiO95257.
    CleanExiHS_GADD45G.
    GenevestigatoriO95257.

    Organism-specific databases

    HPAiHPA023606.

    Interactioni

    Subunit structurei

    Undergoes concentration-dependent homodimerization, which is required for growth inhibititory activity and enhances interaction with PCNA. Interacts with GADD45GIP1. Interacts with PCNA.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GADD45GIP1Q8TAE84EBI-448202,EBI-372506
    KAT5Q929932EBI-448202,EBI-399080
    KIAA1377Q9P2H02EBI-448202,EBI-473176
    MED31Q9Y3C72EBI-448202,EBI-394707
    UTP14AQ9BVJ63EBI-448202,EBI-473284

    Protein-protein interaction databases

    BioGridi116117. 52 interactions.
    DIPiDIP-24218N.
    IntActiO95257. 40 interactions.
    MINTiMINT-265043.
    STRINGi9606.ENSP00000252506.

    Structurei

    Secondary structure

    1
    159
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi17 – 3519
    Beta strandi39 – 424
    Helixi43 – 5210
    Helixi54 – 563
    Beta strandi57 – 637
    Helixi66 – 683
    Helixi72 – 8716
    Beta strandi91 – 966
    Helixi98 – 1058
    Beta strandi111 – 1133
    Beta strandi118 – 1236
    Beta strandi125 – 1284
    Helixi133 – 14715

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2WALX-ray2.40A/B1-159[»]
    3FFMX-ray2.30A1-159[»]
    ProteinModelPortaliO95257.
    SMRiO95257. Positions 15-159.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95257.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni43 – 8644HomodimerizationAdd
    BLAST

    Domaini

    Two central helices mediate homodimerization through parallel packing.

    Sequence similaritiesi

    Belongs to the GADD45 family.Curated

    Phylogenomic databases

    eggNOGiNOG79725.
    HOGENOMiHOG000013221.
    HOVERGENiHBG051684.
    InParanoidiO95257.
    KOiK04402.
    OMAiDWVPTIT.
    OrthoDBiEOG7J1819.
    PhylomeDBiO95257.
    TreeFamiTF300196.

    Family and domain databases

    InterProiIPR024824. GADD45.
    IPR029064. L30e-like.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    [Graphical view]
    PANTHERiPTHR10411. PTHR10411. 1 hit.
    PfamiPF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view]
    SUPFAMiSSF55315. SSF55315. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O95257-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLEEVRGQD TVPESTARMQ GAGKALHELL LSAQRQGCLT AGVYESAKVL    50
    NVDPDNVTFC VLAAGEEDEG DIALQIHFTL IQAFCCENDI DIVRVGDVQR 100
    LAAIVGAGEE AGAPGDLHCI LISNPNEDAW KDPALEKLSL FCEESRSVND 150
    WVPSITLPE 159
    Length:159
    Mass (Da):17,121
    Last modified:May 1, 1999 - v1
    Checksum:i26427E5881941E64
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 352QR → HG in AAK00414. (PubMed:10773677)Curated
    Sequence conflicti113 – 1153APG → CAC in AAK00414. (PubMed:10773677)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti112 – 1121G → S.2 Publications
    Corresponds to variant rs3138505 [ dbSNP | Ensembl ].
    VAR_018888

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF078078 mRNA. Translation: AAC83329.1.
    AF079806 mRNA. Translation: AAD28544.1.
    AF265659 Genomic DNA. Translation: AAF73468.1.
    AF087883 mRNA. Translation: AAK00414.1.
    AK313689 mRNA. Translation: BAG36438.1.
    BT007234 mRNA. Translation: AAP35898.1.
    AF494037 Genomic DNA. Translation: AAM00007.1.
    CH471089 Genomic DNA. Translation: EAW62774.1.
    BC000465 mRNA. Translation: AAH00465.1.
    BC019325 mRNA. Translation: AAH19325.1.
    CCDSiCCDS6686.1.
    RefSeqiNP_006696.1. NM_006705.3.
    UniGeneiHs.9701.

    Genome annotation databases

    EnsembliENST00000252506; ENSP00000252506; ENSG00000130222.
    GeneIDi10912.
    KEGGihsa:10912.
    UCSCiuc004aqq.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs
    Wikipedia

    GADD45G entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF078078 mRNA. Translation: AAC83329.1 .
    AF079806 mRNA. Translation: AAD28544.1 .
    AF265659 Genomic DNA. Translation: AAF73468.1 .
    AF087883 mRNA. Translation: AAK00414.1 .
    AK313689 mRNA. Translation: BAG36438.1 .
    BT007234 mRNA. Translation: AAP35898.1 .
    AF494037 Genomic DNA. Translation: AAM00007.1 .
    CH471089 Genomic DNA. Translation: EAW62774.1 .
    BC000465 mRNA. Translation: AAH00465.1 .
    BC019325 mRNA. Translation: AAH19325.1 .
    CCDSi CCDS6686.1.
    RefSeqi NP_006696.1. NM_006705.3.
    UniGenei Hs.9701.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2WAL X-ray 2.40 A/B 1-159 [» ]
    3FFM X-ray 2.30 A 1-159 [» ]
    ProteinModelPortali O95257.
    SMRi O95257. Positions 15-159.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116117. 52 interactions.
    DIPi DIP-24218N.
    IntActi O95257. 40 interactions.
    MINTi MINT-265043.
    STRINGi 9606.ENSP00000252506.

    Proteomic databases

    PaxDbi O95257.
    PRIDEi O95257.

    Protocols and materials databases

    DNASUi 10912.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000252506 ; ENSP00000252506 ; ENSG00000130222 .
    GeneIDi 10912.
    KEGGi hsa:10912.
    UCSCi uc004aqq.3. human.

    Organism-specific databases

    CTDi 10912.
    GeneCardsi GC09P092219.
    H-InvDB HIX0169359.
    HGNCi HGNC:4097. GADD45G.
    HPAi HPA023606.
    MIMi 604949. gene.
    neXtProti NX_O95257.
    PharmGKBi PA28512.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG79725.
    HOGENOMi HOG000013221.
    HOVERGENi HBG051684.
    InParanoidi O95257.
    KOi K04402.
    OMAi DWVPTIT.
    OrthoDBi EOG7J1819.
    PhylomeDBi O95257.
    TreeFami TF300196.

    Enzyme and pathway databases

    SignaLinki O95257.

    Miscellaneous databases

    ChiTaRSi GADD45G. human.
    EvolutionaryTracei O95257.
    GeneWikii GADD45G.
    GenomeRNAii 10912.
    NextBioi 41445.
    PROi O95257.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95257.
    Bgeei O95257.
    CleanExi HS_GADD45G.
    Genevestigatori O95257.

    Family and domain databases

    InterProi IPR024824. GADD45.
    IPR029064. L30e-like.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    [Graphical view ]
    PANTHERi PTHR10411. PTHR10411. 1 hit.
    Pfami PF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55315. SSF55315. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK."
      Takekawa M., Saito H.
      Cell 95:521-530(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cytokine response gene 6 induces p21 and regulates both cell growth and arrest."
      Fan W., Richter G., Cereseto A., Beadling C., Smith K.A.
      Oncogene 18:6573-6582(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Cloning and characterization of Gadd45 gamma gene and its regulation by C/EBP."
      Jung N., Yi Y., Kim D., Shong M., Hong S., Lee H., Bae I.
      Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Assignment of human GADD45G to chromosome 9q22.1-->q22.3 by radiation hybrid mapping."
      Gong R., Yu L., Zhang H., Tu Q., Zhao Y., Yang J., Xu Y., Zhao S.
      Cytogenet. Cell Genet. 88:95-96(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-112.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. NIEHS SNPs program
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-112.
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    10. "CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle."
      Chung H.K., Yi Y.-W., Jung N.-C., Kim D., Suh J.M., Kim H., Park K.C., Song J.H., Kim D.W., Hwang E.S., Yoon S.-H., Bae Y.-S., Kim J.M., Bae I., Shong M.
      J. Biol. Chem. 278:28079-28088(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GADD45GIP1.
      Tissue: Colon carcinoma.
    11. "Crystal structure of human Gadd45gamma reveals an active dimer."
      Zhang W., Fu S., Liu X., Zhao X., Zhang W., Peng W., Wu C., Li Y., Li X., Bartlam M., Zeng Z.H., Zhan Q., Rao Z.
      Protein Cell 2:814-826(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ALA-47; ILE-76; LEU-80; ALA-83; GLU-87 AND ASP-89, INTERACTION WITH PCNA.

    Entry informationi

    Entry nameiGA45G_HUMAN
    AccessioniPrimary (citable) accession number: O95257
    Secondary accession number(s): Q5VZ87, Q9C076
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3