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O95257 (GA45G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Growth arrest and DNA damage-inducible protein GADD45 gamma
Alternative name(s):
Cytokine-responsive protein CR6
DNA damage-inducible transcript 2 protein
Short name=DDIT-2
Gene names
Name:GADD45G
Synonyms:CR6, DDIT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.

Subunit structure

Undergoes concentration-dependent homodimerization, which is required for growth inhibititory activity and enhances interaction with PCNA. Interacts with GADD45GIP1. Interacts with PCNA. Ref.10 Ref.11

Domain

Two central helices mediate homodimerization through parallel packing.

Sequence similarities

Belongs to the GADD45 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159Growth arrest and DNA damage-inducible protein GADD45 gamma
PRO_0000148336

Regions

Region43 – 8644Homodimerization

Natural variations

Natural variant1121G → S. Ref.4 Ref.7
Corresponds to variant rs3138505 [ dbSNP | Ensembl ].
VAR_018888

Experimental info

Mutagenesis471A → R: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with E-77, E-80 and R-83. Ref.11
Mutagenesis761I → E: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-80 and R-83. Ref.11
Mutagenesis801L → E: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-77 and R-83. Ref.11
Mutagenesis831A → R: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-77 and E-80. Ref.11
Mutagenesis871E → K: Reduced growth inhibition activity; when associated with K-89. Ref.11
Mutagenesis891D → K: Reduced growth inhibition activity; when associated with K-87. Ref.11
Sequence conflict34 – 352QR → HG in AAK00414. Ref.4
Sequence conflict113 – 1153APG → CAC in AAK00414. Ref.4

Secondary structure

......................... 159
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95257 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 26427E5881941E64

FASTA15917,121
        10         20         30         40         50         60 
MTLEEVRGQD TVPESTARMQ GAGKALHELL LSAQRQGCLT AGVYESAKVL NVDPDNVTFC 

        70         80         90        100        110        120 
VLAAGEEDEG DIALQIHFTL IQAFCCENDI DIVRVGDVQR LAAIVGAGEE AGAPGDLHCI 

       130        140        150 
LISNPNEDAW KDPALEKLSL FCEESRSVND WVPSITLPE 

« Hide

References

« Hide 'large scale' references
[1]"A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK."
Takekawa M., Saito H.
Cell 95:521-530(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cytokine response gene 6 induces p21 and regulates both cell growth and arrest."
Fan W., Richter G., Cereseto A., Beadling C., Smith K.A.
Oncogene 18:6573-6582(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning and characterization of Gadd45 gamma gene and its regulation by C/EBP."
Jung N., Yi Y., Kim D., Shong M., Hong S., Lee H., Bae I.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Assignment of human GADD45G to chromosome 9q22.1-->q22.3 by radiation hybrid mapping."
Gong R., Yu L., Zhang H., Tu Q., Zhao Y., Yang J., Xu Y., Zhao S.
Cytogenet. Cell Genet. 88:95-96(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-112.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]NIEHS SNPs program
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-112.
[8]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[10]"CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle."
Chung H.K., Yi Y.-W., Jung N.-C., Kim D., Suh J.M., Kim H., Park K.C., Song J.H., Kim D.W., Hwang E.S., Yoon S.-H., Bae Y.-S., Kim J.M., Bae I., Shong M.
J. Biol. Chem. 278:28079-28088(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GADD45GIP1.
Tissue: Colon carcinoma.
[11]"Crystal structure of human Gadd45gamma reveals an active dimer."
Zhang W., Fu S., Liu X., Zhao X., Zhang W., Peng W., Wu C., Li Y., Li X., Bartlam M., Zeng Z.H., Zhan Q., Rao Z.
Protein Cell 2:814-826(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ALA-47; ILE-76; LEU-80; ALA-83; GLU-87 AND ASP-89, INTERACTION WITH PCNA.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF078078 mRNA. Translation: AAC83329.1.
AF079806 mRNA. Translation: AAD28544.1.
AF265659 Genomic DNA. Translation: AAF73468.1.
AF087883 mRNA. Translation: AAK00414.1.
AK313689 mRNA. Translation: BAG36438.1.
BT007234 mRNA. Translation: AAP35898.1.
AF494037 Genomic DNA. Translation: AAM00007.1.
CH471089 Genomic DNA. Translation: EAW62774.1.
BC000465 mRNA. Translation: AAH00465.1.
BC019325 mRNA. Translation: AAH19325.1.
CCDSCCDS6686.1.
RefSeqNP_006696.1. NM_006705.3.
UniGeneHs.9701.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WALX-ray2.40A/B1-159[»]
3FFMX-ray2.30A1-159[»]
ProteinModelPortalO95257.
SMRO95257. Positions 15-159.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116117. 52 interactions.
DIPDIP-24218N.
IntActO95257. 40 interactions.
MINTMINT-265043.
STRING9606.ENSP00000252506.

Proteomic databases

PaxDbO95257.
PRIDEO95257.

Protocols and materials databases

DNASU10912.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000252506; ENSP00000252506; ENSG00000130222.
GeneID10912.
KEGGhsa:10912.
UCSCuc004aqq.3. human.

Organism-specific databases

CTD10912.
GeneCardsGC09P092219.
H-InvDBHIX0169359.
HGNCHGNC:4097. GADD45G.
HPAHPA023606.
MIM604949. gene.
neXtProtNX_O95257.
PharmGKBPA28512.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG79725.
HOGENOMHOG000013221.
HOVERGENHBG051684.
InParanoidO95257.
KOK04402.
OMADWVPTIT.
OrthoDBEOG7J1819.
PhylomeDBO95257.
TreeFamTF300196.

Enzyme and pathway databases

SignaLinkO95257.

Gene expression databases

ArrayExpressO95257.
BgeeO95257.
CleanExHS_GADD45G.
GenevestigatorO95257.

Family and domain databases

InterProIPR024824. GADD45.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERPTHR10411. PTHR10411. 1 hit.
PfamPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMSSF55315. SSF55315. 1 hit.
ProtoNetSearch...

Other

ChiTaRSGADD45G. human.
EvolutionaryTraceO95257.
GeneWikiGADD45G.
GenomeRNAi10912.
NextBio41445.
PROO95257.
SOURCESearch...

Entry information

Entry nameGA45G_HUMAN
AccessionPrimary (citable) accession number: O95257
Secondary accession number(s): Q5VZ87, Q9C076
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM