Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Growth arrest and DNA damage-inducible protein GADD45 gamma

Gene

GADD45G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.

GO - Biological processi

  • activation of MAPKKK activity Source: UniProtKB
  • apoptotic process Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of JNK cascade Source: UniProtKB
  • positive regulation of p38MAPK cascade Source: UniProtKB
  • regulation of cell cycle Source: GO_Central
  • response to stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis, Differentiation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130222-MONOMER.
SignaLinkiO95257.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth arrest and DNA damage-inducible protein GADD45 gamma
Alternative name(s):
Cytokine-responsive protein CR6
DNA damage-inducible transcript 2 protein
Short name:
DDIT-2
Gene namesi
Name:GADD45G
Synonyms:CR6, DDIT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:4097. GADD45G.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47A → R: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with E-77, E-80 and R-83. 1 Publication1
Mutagenesisi76I → E: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-80 and R-83. 1 Publication1
Mutagenesisi80L → E: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-77 and R-83. 1 Publication1
Mutagenesisi83A → R: 30-fold reduction in homodimerization affinity and 90% decrease in growth inhibition activity and ability to stop cell cycle; when associated with R-47, E-77 and E-80. 1 Publication1
Mutagenesisi87E → K: Reduced growth inhibition activity; when associated with K-89. 1 Publication1
Mutagenesisi89D → K: Reduced growth inhibition activity; when associated with K-87. 1 Publication1

Organism-specific databases

DisGeNETi10912.
OpenTargetsiENSG00000130222.
PharmGKBiPA28512.

Polymorphism and mutation databases

BioMutaiGADD45G.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001483361 – 159Growth arrest and DNA damage-inducible protein GADD45 gammaAdd BLAST159

Proteomic databases

PaxDbiO95257.
PRIDEiO95257.

Expressioni

Gene expression databases

BgeeiENSG00000130222.
CleanExiHS_GADD45G.
ExpressionAtlasiO95257. baseline and differential.
GenevisibleiO95257. HS.

Organism-specific databases

HPAiHPA023606.

Interactioni

Subunit structurei

Undergoes concentration-dependent homodimerization, which is required for growth inhibititory activity and enhances interaction with PCNA. Interacts with GADD45GIP1. Interacts with PCNA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AFAP1L1Q8TED95EBI-448202,EBI-1053644
CEP126Q9P2H02EBI-448202,EBI-473176
CEP57L1Q8IYX8-23EBI-448202,EBI-10181988
GADD45GIP1Q8TAE84EBI-448202,EBI-372506
KAT5Q929932EBI-448202,EBI-399080
MED31Q9Y3C72EBI-448202,EBI-394707
RELQ048643EBI-448202,EBI-307352
RWDD2BP5706010EBI-448202,EBI-724442
TSNAXQ995983EBI-448202,EBI-742638
UTP14AQ9BVJ63EBI-448202,EBI-473284

Protein-protein interaction databases

BioGridi116117. 54 interactors.
DIPiDIP-24218N.
IntActiO95257. 46 interactors.
MINTiMINT-265043.
STRINGi9606.ENSP00000252506.

Structurei

Secondary structure

1159
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 35Combined sources19
Beta strandi39 – 42Combined sources4
Helixi43 – 52Combined sources10
Helixi54 – 56Combined sources3
Beta strandi57 – 63Combined sources7
Helixi66 – 68Combined sources3
Helixi72 – 87Combined sources16
Beta strandi91 – 96Combined sources6
Helixi98 – 105Combined sources8
Beta strandi111 – 113Combined sources3
Beta strandi118 – 123Combined sources6
Beta strandi125 – 128Combined sources4
Helixi133 – 147Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WALX-ray2.40A/B1-159[»]
3FFMX-ray2.30A1-159[»]
ProteinModelPortaliO95257.
SMRiO95257.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95257.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 86HomodimerizationAdd BLAST44

Domaini

Two central helices mediate homodimerization through parallel packing.

Sequence similaritiesi

Belongs to the GADD45 family.Curated

Phylogenomic databases

eggNOGiENOG410IW58. Eukaryota.
ENOG4111FXB. LUCA.
GeneTreeiENSGT00390000016506.
HOGENOMiHOG000013221.
HOVERGENiHBG051684.
InParanoidiO95257.
KOiK04402.
OMAiDWVPTIT.
OrthoDBiEOG091G0RHU.
PhylomeDBiO95257.
TreeFamiTF300196.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR024824. GADD45.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR10411. PTHR10411. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.

Sequencei

Sequence statusi: Complete.

O95257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLEEVRGQD TVPESTARMQ GAGKALHELL LSAQRQGCLT AGVYESAKVL
60 70 80 90 100
NVDPDNVTFC VLAAGEEDEG DIALQIHFTL IQAFCCENDI DIVRVGDVQR
110 120 130 140 150
LAAIVGAGEE AGAPGDLHCI LISNPNEDAW KDPALEKLSL FCEESRSVND

WVPSITLPE
Length:159
Mass (Da):17,121
Last modified:May 1, 1999 - v1
Checksum:i26427E5881941E64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34 – 35QR → HG in AAK00414 (PubMed:10773677).Curated2
Sequence conflicti113 – 115APG → CAC in AAK00414 (PubMed:10773677).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018888112G → S.2 PublicationsCorresponds to variant rs3138505dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078078 mRNA. Translation: AAC83329.1.
AF079806 mRNA. Translation: AAD28544.1.
AF265659 Genomic DNA. Translation: AAF73468.1.
AF087883 mRNA. Translation: AAK00414.1.
AK313689 mRNA. Translation: BAG36438.1.
BT007234 mRNA. Translation: AAP35898.1.
AF494037 Genomic DNA. Translation: AAM00007.1.
CH471089 Genomic DNA. Translation: EAW62774.1.
BC000465 mRNA. Translation: AAH00465.1.
BC019325 mRNA. Translation: AAH19325.1.
CCDSiCCDS6686.1.
RefSeqiNP_006696.1. NM_006705.3.
UniGeneiHs.9701.

Genome annotation databases

EnsembliENST00000252506; ENSP00000252506; ENSG00000130222.
GeneIDi10912.
KEGGihsa:10912.
UCSCiuc004aqq.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs
Wikipedia

GADD45G entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078078 mRNA. Translation: AAC83329.1.
AF079806 mRNA. Translation: AAD28544.1.
AF265659 Genomic DNA. Translation: AAF73468.1.
AF087883 mRNA. Translation: AAK00414.1.
AK313689 mRNA. Translation: BAG36438.1.
BT007234 mRNA. Translation: AAP35898.1.
AF494037 Genomic DNA. Translation: AAM00007.1.
CH471089 Genomic DNA. Translation: EAW62774.1.
BC000465 mRNA. Translation: AAH00465.1.
BC019325 mRNA. Translation: AAH19325.1.
CCDSiCCDS6686.1.
RefSeqiNP_006696.1. NM_006705.3.
UniGeneiHs.9701.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WALX-ray2.40A/B1-159[»]
3FFMX-ray2.30A1-159[»]
ProteinModelPortaliO95257.
SMRiO95257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116117. 54 interactors.
DIPiDIP-24218N.
IntActiO95257. 46 interactors.
MINTiMINT-265043.
STRINGi9606.ENSP00000252506.

Polymorphism and mutation databases

BioMutaiGADD45G.

Proteomic databases

PaxDbiO95257.
PRIDEiO95257.

Protocols and materials databases

DNASUi10912.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252506; ENSP00000252506; ENSG00000130222.
GeneIDi10912.
KEGGihsa:10912.
UCSCiuc004aqq.4. human.

Organism-specific databases

CTDi10912.
DisGeNETi10912.
GeneCardsiGADD45G.
H-InvDBHIX0169359.
HGNCiHGNC:4097. GADD45G.
HPAiHPA023606.
MIMi604949. gene.
neXtProtiNX_O95257.
OpenTargetsiENSG00000130222.
PharmGKBiPA28512.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IW58. Eukaryota.
ENOG4111FXB. LUCA.
GeneTreeiENSGT00390000016506.
HOGENOMiHOG000013221.
HOVERGENiHBG051684.
InParanoidiO95257.
KOiK04402.
OMAiDWVPTIT.
OrthoDBiEOG091G0RHU.
PhylomeDBiO95257.
TreeFamiTF300196.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130222-MONOMER.
SignaLinkiO95257.

Miscellaneous databases

ChiTaRSiGADD45G. human.
EvolutionaryTraceiO95257.
GeneWikiiGADD45G.
GenomeRNAii10912.
PROiO95257.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130222.
CleanExiHS_GADD45G.
ExpressionAtlasiO95257. baseline and differential.
GenevisibleiO95257. HS.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR024824. GADD45.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR10411. PTHR10411. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGA45G_HUMAN
AccessioniPrimary (citable) accession number: O95257
Secondary accession number(s): Q5VZ87, Q9C076
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.