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Protein

Golgi SNAP receptor complex member 1

Gene

GOSR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.2 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: HGNC
  • SNARE binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi SNAP receptor complex member 1
Alternative name(s):
28 kDa Golgi SNARE protein
28 kDa cis-Golgi SNARE p28
Short name:
GOS-28
Gene namesi
Name:GOSR1
Synonyms:GS28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:4430. GOSR1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 229228CytoplasmicSequence analysisAdd
BLAST
Transmembranei230 – 25021Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST

GO - Cellular componenti

  • cis-Golgi network Source: GO_Central
  • cytosol Source: GOC
  • Golgi apparatus Source: UniProtKB
  • Golgi medial cisterna Source: GO_Central
  • Golgi membrane Source: BHF-UCL
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • SNARE complex Source: HGNC
  • transport vesicle Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28815.

Polymorphism and mutation databases

BioMutaiGOSR1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 250249Golgi SNAP receptor complex member 1PRO_0000212542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei141 – 1411PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO95249.
MaxQBiO95249.
PaxDbiO95249.
PeptideAtlasiO95249.
PRIDEiO95249.

PTM databases

iPTMnetiO95249.
PhosphoSiteiO95249.

Expressioni

Inductioni

Expression induced by hydrogen peroxide in neuronal cells. By monocrotaline in pulmonary epithelial cells (at protein level). Negatively regulated by OSBPL7 via GABARAPL2 leading to degradation on proteasomes (at protein level).3 Publications

Gene expression databases

BgeeiO95249.
CleanExiHS_GOSR1.
ExpressionAtlasiO95249. baseline and differential.
GenevisibleiO95249. HS.

Organism-specific databases

HPAiHPA020590.

Interactioni

Subunit structurei

Component of several multiprotein Golgi SNARE complexes. Identified in a SNARE complex with BET1, STX5 and YKT6, in a SNARE complex with BET1L, STX5 and YKT6, in a SNARE complex with STX5, GOSR2, SEC22B and BET1, and in complex with STX5 and COG3. Interacts with GABARAPL2 (By similarity).By similarity

GO - Molecular functioni

  • SNAP receptor activity Source: HGNC
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi114903. 41 interactions.
IntActiO95249. 7 interactions.
STRINGi9606.ENSP00000225724.

Structurei

3D structure databases

ProteinModelPortaliO95249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili9 – 3022Sequence analysisAdd
BLAST
Coiled coili68 – 9528Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the GOSR1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3208. Eukaryota.
ENOG410YI1X. LUCA.
GeneTreeiENSGT00390000008688.
HOGENOMiHOG000207760.
HOVERGENiHBG051764.
InParanoidiO95249.
KOiK08495.
PhylomeDBiO95249.
TreeFamiTF105782.

Family and domain databases

InterProiIPR023601. Golgi_SNAP_su1.
[Graphical view]
PANTHERiPTHR21094. PTHR21094. 1 hit.
PIRSFiPIRSF027109. Golgi_SNARE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95249-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGTSSYWE DLRKQARQLE NELDLKLVSF SKLCTSYSHS STRDGRRDRY
60 70 80 90 100
SSDTTPLLNG SSQDRMFETM AIEIEQLLAR LTGVNDKMAE YTNSAGVPSL
110 120 130 140 150
NAALMHTLQR HRDILQDYTH EFHKTKANFM AIRERENLMG SVRKDIESYK
160 170 180 190 200
SGSGVNNRRT ELFLKEHDHL RNSDRLIEET ISIAMATKEN MTSQRGMLKS
210 220 230 240 250
IHSKMNTLAN RFPAVNSLIQ RINLRKRRDS LILGGVIGIC TILLLLYAFH
Length:250
Mass (Da):28,613
Last modified:May 1, 1999 - v1
Checksum:iEEDA4291436162F6
GO
Isoform 2 (identifier: O95249-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: Gene prediction based on EST data.
Show »
Length:185
Mass (Da):21,214
Checksum:i2FF672E5364FC52A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111D → GEARRPPD in AAC39889 (PubMed:9653160).Curated
Sequence conflicti49 – 502Missing in AAC39889 (PubMed:9653160).Curated
Sequence conflicti156 – 1572NN → TT in AAC39889 (PubMed:9653160).Curated
Sequence conflicti159 – 1591R → G in AAC39889 (PubMed:9653160).Curated
Sequence conflicti170 – 1701L → F in AAC39889 (PubMed:9653160).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6565Missing in isoform 2. CuratedVSP_047326Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047438 mRNA. Translation: AAC39889.1.
AF073926 mRNA. Translation: AAD12945.1.
AC006050 Genomic DNA. No translation available.
AC011840 Genomic DNA. No translation available.
CH471159 Genomic DNA. Translation: EAW51229.1.
BC040471 mRNA. Translation: AAH40471.1.
CCDSiCCDS11258.1. [O95249-1]
CCDS45644.1. [O95249-2]
RefSeqiNP_001007025.1. NM_001007024.1. [O95249-2]
NP_001007026.1. NM_001007025.1.
NP_004862.1. NM_004871.2. [O95249-1]
XP_005258129.1. XM_005258072.1. [O95249-2]
UniGeneiHs.462680.

Genome annotation databases

EnsembliENST00000225724; ENSP00000225724; ENSG00000108587. [O95249-1]
ENST00000467337; ENSP00000462638; ENSG00000108587. [O95249-2]
GeneIDi9527.
KEGGihsa:9527.
UCSCiuc002hfe.4. human. [O95249-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047438 mRNA. Translation: AAC39889.1.
AF073926 mRNA. Translation: AAD12945.1.
AC006050 Genomic DNA. No translation available.
AC011840 Genomic DNA. No translation available.
CH471159 Genomic DNA. Translation: EAW51229.1.
BC040471 mRNA. Translation: AAH40471.1.
CCDSiCCDS11258.1. [O95249-1]
CCDS45644.1. [O95249-2]
RefSeqiNP_001007025.1. NM_001007024.1. [O95249-2]
NP_001007026.1. NM_001007025.1.
NP_004862.1. NM_004871.2. [O95249-1]
XP_005258129.1. XM_005258072.1. [O95249-2]
UniGeneiHs.462680.

3D structure databases

ProteinModelPortaliO95249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114903. 41 interactions.
IntActiO95249. 7 interactions.
STRINGi9606.ENSP00000225724.

PTM databases

iPTMnetiO95249.
PhosphoSiteiO95249.

Polymorphism and mutation databases

BioMutaiGOSR1.

Proteomic databases

EPDiO95249.
MaxQBiO95249.
PaxDbiO95249.
PeptideAtlasiO95249.
PRIDEiO95249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225724; ENSP00000225724; ENSG00000108587. [O95249-1]
ENST00000467337; ENSP00000462638; ENSG00000108587. [O95249-2]
GeneIDi9527.
KEGGihsa:9527.
UCSCiuc002hfe.4. human. [O95249-1]

Organism-specific databases

CTDi9527.
GeneCardsiGOSR1.
HGNCiHGNC:4430. GOSR1.
HPAiHPA020590.
MIMi604026. gene.
neXtProtiNX_O95249.
PharmGKBiPA28815.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3208. Eukaryota.
ENOG410YI1X. LUCA.
GeneTreeiENSGT00390000008688.
HOGENOMiHOG000207760.
HOVERGENiHBG051764.
InParanoidiO95249.
KOiK08495.
PhylomeDBiO95249.
TreeFamiTF105782.

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811438. Intra-Golgi traffic.

Miscellaneous databases

ChiTaRSiGOSR1. human.
GeneWikiiGOSR1.
GenomeRNAii9527.
PROiO95249.
SOURCEiSearch...

Gene expression databases

BgeeiO95249.
CleanExiHS_GOSR1.
ExpressionAtlasiO95249. baseline and differential.
GenevisibleiO95249. HS.

Family and domain databases

InterProiIPR023601. Golgi_SNAP_su1.
[Graphical view]
PANTHERiPTHR21094. PTHR21094. 1 hit.
PIRSFiPIRSF027109. Golgi_SNARE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."
    Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.
    Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Umbilical cord blood.
  2. "cDNA characterization and chromosomal mapping of human Golgi SNARE GS27 and GS28 to chromosome 17."
    Bui T.D., Levy E.R., Subramaniam V.N., Lowe S.L., Hong W.
    Genomics 57:285-288(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Colon carcinoma.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  6. "Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex in transport from the early/recycling endosome to the trans-Golgi network."
    Tai G., Lu L., Wang T.L., Tang B.L., Goud B., Johannes L., Hong W.
    Mol. Biol. Cell 15:4011-4022(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells."
    Zolov S.N., Lupashin V.V.
    J. Cell Biol. 168:747-759(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Low-temperature-induced Golgi tubules are transient membranes enriched in molecules regulating intra-Golgi transport."
    Martinez-Alonso E., Ballesta J., Martinez-Menarguez J.A.
    Traffic 8:359-368(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Dysfunction of Golgi tethers, SNAREs, and SNAPs in monocrotaline-induced pulmonary hypertension."
    Sehgal P.B., Mukhopadhyay S., Xu F., Patel K., Shah M.
    Am. J. Physiol. 292:L1526-L1542(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION BY MONOCROTALINE.
  10. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "OSBP-related protein 7 interacts with GATE-16 and negatively regulates GS28 protein stability."
    Zhong W., Zhou Y., Li S., Zhou T., Ma H., Wei K., Li H., Olkkonen V.M., Yan D.
    Exp. Cell Res. 317:2353-2363(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION BY OSBPL7.
  13. "GS28 protects neuronal cell death induced by hydrogen peroxide under glutathione-depleted condition."
    Lee H.O., Byun Y.J., Cho K.O., Kim S.Y., Lee S.B., Kim H.S., Kwon O.J., Jeong S.W.
    Korean J. Physiol. Pharmacol. 15:149-156(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  14. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Erythroleukemia.
  16. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGOSR1_HUMAN
AccessioniPrimary (citable) accession number: O95249
Secondary accession number(s): J3KST5, O75392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.