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Protein

Chromosome-associated kinesin KIF4A

Gene

KIF4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 95ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • anterograde axonal transport Source: ProtInc
  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  • microtubule-based movement Source: Reactome
  • mitotic cytokinesis Source: UniProtKB
  • mitotic spindle midzone assembly Source: UniProtKB
  • organelle organization Source: ProtInc
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000090889-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-437239. Recycling pathway of L1.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome-associated kinesin KIF4A
Alternative name(s):
Chromokinesin-A
Gene namesi
Name:KIF4A
Synonyms:KIF4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:13339. KIF4A.

Subcellular locationi

  • Nucleus matrix
  • Cytoplasmcytoskeletonspindle
  • Midbody
  • Chromosome

  • Note: Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.

GO - Cellular componenti

  • axon cytoplasm Source: GOC
  • chromosome Source: UniProtKB-SubCell
  • cytoplasm Source: ProtInc
  • cytosol Source: Reactome
  • kinesin complex Source: GO_Central
  • membrane Source: UniProtKB
  • midbody Source: UniProtKB
  • nuclear matrix Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • spindle microtubule Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi24137.
MalaCardsiKIF4A.
OpenTargetsiENSG00000090889.
PharmGKBiPA30105.

Chemistry databases

ChEMBLiCHEMBL6163.

Polymorphism and mutation databases

BioMutaiKIF4A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001254371 – 1232Chromosome-associated kinesin KIF4AAdd BLAST1232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei394PhosphoserineBy similarity1
Modified residuei799PhosphothreonineCombined sources1
Modified residuei801PhosphoserineCombined sources1
Modified residuei810PhosphoserineBy similarity1
Modified residuei815PhosphoserineCombined sources1
Modified residuei951PhosphoserineCombined sources1
Modified residuei995PhosphothreonineCombined sources1
Modified residuei1001PhosphoserineCombined sources1
Modified residuei1013PhosphoserineCombined sources1
Modified residuei1017PhosphoserineCombined sources1
Modified residuei1028PhosphoserineCombined sources1
Modified residuei1126PhosphoserineCombined sources1
Modified residuei1181PhosphothreonineCombined sources1
Modified residuei1186PhosphoserineCombined sources1
Modified residuei1225PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO95239.
MaxQBiO95239.
PaxDbiO95239.
PeptideAtlasiO95239.
PRIDEiO95239.

PTM databases

iPTMnetiO95239.
PhosphoSitePlusiO95239.

Expressioni

Tissue specificityi

Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.1 Publication

Gene expression databases

BgeeiENSG00000090889.
CleanExiHS_KIF4A.
GenevisibleiO95239. HS.

Organism-specific databases

HPAiHPA034745.
HPA034746.

Interactioni

Subunit structurei

Interacts with unphosphorylated PRC1 during late mitosis.2 Publications

Protein-protein interaction databases

BioGridi117288. 22 interactors.
IntActiO95239. 8 interactors.
MINTiMINT-3002743.
STRINGi9606.ENSP00000363524.

Structurei

3D structure databases

ProteinModelPortaliO95239.
SMRiO95239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 336Kinesin motorPROSITE-ProRule annotationAdd BLAST328

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni663 – 1232Interaction with PRC1Add BLAST570
Regioni1000 – 1232GlobularAdd BLAST233

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili350 – 999Sequence analysisAdd BLAST650

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi793 – 798Nuclear localization signal6

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOVERGENiHBG052256.
InParanoidiO95239.
KOiK10395.
OMAiRGMEGTT.
OrthoDBiEOG091G02I4.
PhylomeDBiO95239.
TreeFamiTF105224.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95239-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKEEVKGIPV RVALRCRPLV PKEISEGCQM CLSFVPGEPQ VVVGTDKSFT
60 70 80 90 100
YDFVFDPSTE QEEVFNTAVA PLIKGVFKGY NATVLAYGQT GSGKTYSMGG
110 120 130 140 150
AYTAEQENEP TVGVIPRVIQ LLFKEIDKKS DFEFTLKVSY LEIYNEEILD
160 170 180 190 200
LLCPSREKAQ INIREDPKEG IKIVGLTEKT VLVALDTVSC LEQGNNSRTV
210 220 230 240 250
ASTAMNSQSS RSHAIFTISL EQRKKSDKNS SFRSKLHLVD LAGSERQKKT
260 270 280 290 300
KAEGDRLKEG ININRGLLCL GNVISALGDD KKGGFVPYRD SKLTRLLQDS
310 320 330 340 350
LGGNSHTLMI ACVSPADSNL EETLNTLRYA DRARKIKNKP IVNIDPQTAE
360 370 380 390 400
LNHLKQQVQQ LQVLLLQAHG GTLPGSITVE PSENLQSLME KNQSLVEENE
410 420 430 440 450
KLSRGLSEAA GQTAQMLERI ILTEQANEKM NAKLEELRQH AACKLDLQKL
460 470 480 490 500
VETLEDQELK ENVEIICNLQ QLITQLSDET VACMAAAIDT AVEQEAQVET
510 520 530 540 550
SPETSRSSDA FTTQHALRQA QMSKELVELN KALALKEALA RKMTQNDSQL
560 570 580 590 600
QPIQYQYQDN IKELELEVIN LQKEKEELVL ELQTAKKDAN QAKLSERRRK
610 620 630 640 650
RLQELEGQIA DLKKKLNEQS KLLKLKESTE RTVSKLNQEI RMMKNQRVQL
660 670 680 690 700
MRQMKEDAEK FRQWKQKKDK EVIQLKERDR KRQYELLKLE RNFQKQSNVL
710 720 730 740 750
RRKTEEAAAA NKRLKDALQK QREVADKRKE TQSRGMEGTA ARVKNWLGNE
760 770 780 790 800
IEVMVSTEEA KRHLNDLLED RKILAQDVAQ LKEKKESGEN PPPKLRRRTF
810 820 830 840 850
SLTEVRGQVS ESEDSITKQI ESLETEMEFR SAQIADLQQK LLDAESEDRP
860 870 880 890 900
KQRWENIATI LEAKCALKYL IGELVSSKIQ VSKLESSLKQ SKTSCADMQK
910 920 930 940 950
MLFEERNHFA EIETELQAEL VRMEQQHQEK VLYLLSQLQQ SQMAEKQLEE
960 970 980 990 1000
SVSEKEQQLL STLKCQDEEL EKMREVCEQN QQLLRENEII KQKLTLLQVA
1010 1020 1030 1040 1050
SRQKHLPKDT LLSPDSSFEY VPPKPKPSRV KEKFLEQSMD IEDLKYCSEH
1060 1070 1080 1090 1100
SVNEHEDGDG DDDEGDDEEW KPTKLVKVSR KNIQGCSCKG WCGNKQCGCR
1110 1120 1130 1140 1150
KQKSDCGVDC CCDPTKCRNR QQGKDSLGTV ERTQDSEGSF KLEDPTEVTP
1160 1170 1180 1190 1200
GLSFFNPVCA TPNSKILKEM CDVEQVLSKK TPPAPSPFDL PELKHVATEY
1210 1220 1230
QENKAPGKKK KRALASNTSF FSGCSPIEEE AH
Length:1,232
Mass (Da):139,881
Last modified:April 12, 2005 - v3
Checksum:iAD1B7C2A10AB24DB
GO
Isoform 2 (identifier: O95239-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1086-1127: CSCKGWCGNK...RNRQQGKDSL → VLLLTPVISA...RDPVSTKPKN
     1128-1232: Missing.

Note: No experimental confirmation available.
Show »
Length:1,127
Mass (Da):128,459
Checksum:i84D7ABE2CE39697A
GO

Sequence cautioni

The sequence AAH49218 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223R → G in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti231S → T in AAF86334 (PubMed:9168136).Curated1
Sequence conflicti286V → A in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti564L → H in AAF86334 (PubMed:9168136).Curated1
Sequence conflicti564L → P in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti600K → E in CAB75427 (Ref. 3) Curated1
Sequence conflicti668K → R (Ref. 1) Curated1
Sequence conflicti668K → R (PubMed:10978527).Curated1
Sequence conflicti928Q → P in AAD51855 (Ref. 1) Curated1
Sequence conflicti958Q → R in CAB75427 (Ref. 3) Curated1
Sequence conflicti960L → Q in AAD51855 (Ref. 1) Curated1
Sequence conflicti996 – 997LL → S in AAF86334 (PubMed:9168136).Curated2
Sequence conflicti1003 – 1014QKHLP…TLLSP → RTLPRIPFYLQ in AAF86334 (PubMed:9168136).CuratedAdd BLAST12
Sequence conflicti1022P → Q in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti1077K → N in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti1138G → S in AAD05492 (PubMed:10978527).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021828422L → W.2 PublicationsCorresponds to variant rs1199457dbSNPEnsembl.1
Natural variantiVAR_049693491A → V.Corresponds to variant rs2297871dbSNPEnsembl.1
Natural variantiVAR_0496941193L → S.Corresponds to variant rs1046485dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0133751086 – 1127CSCKG…GKDSL → VLLLTPVISALWEAEARGLL EARSSRPAWATWRDPVSTKP KN in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_0133761128 – 1232Missing in isoform 2. 1 PublicationAdd BLAST105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179308 mRNA. Translation: AAD51855.1.
AF071592 mRNA. Translation: AAD05492.2.
AJ271784 mRNA. Translation: CAB75427.1.
AK313133 mRNA. Translation: BAG35952.1.
AL139398, AL357752 Genomic DNA. Translation: CAI41024.1.
AL357752, AL139398 Genomic DNA. Translation: CAI41487.1.
CH471132 Genomic DNA. Translation: EAX05341.1.
CH471132 Genomic DNA. Translation: EAX05342.1.
BC049218 mRNA. Translation: AAH49218.1. Sequence problems.
BC050548 mRNA. Translation: AAH50548.1.
AF277375 mRNA. Translation: AAF86334.1.
CCDSiCCDS14401.1. [O95239-1]
RefSeqiNP_036442.3. NM_012310.4. [O95239-1]
UniGeneiHs.648326.

Genome annotation databases

EnsembliENST00000374403; ENSP00000363524; ENSG00000090889. [O95239-1]
GeneIDi24137.
KEGGihsa:24137.
UCSCiuc004dyg.4. human. [O95239-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179308 mRNA. Translation: AAD51855.1.
AF071592 mRNA. Translation: AAD05492.2.
AJ271784 mRNA. Translation: CAB75427.1.
AK313133 mRNA. Translation: BAG35952.1.
AL139398, AL357752 Genomic DNA. Translation: CAI41024.1.
AL357752, AL139398 Genomic DNA. Translation: CAI41487.1.
CH471132 Genomic DNA. Translation: EAX05341.1.
CH471132 Genomic DNA. Translation: EAX05342.1.
BC049218 mRNA. Translation: AAH49218.1. Sequence problems.
BC050548 mRNA. Translation: AAH50548.1.
AF277375 mRNA. Translation: AAF86334.1.
CCDSiCCDS14401.1. [O95239-1]
RefSeqiNP_036442.3. NM_012310.4. [O95239-1]
UniGeneiHs.648326.

3D structure databases

ProteinModelPortaliO95239.
SMRiO95239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117288. 22 interactors.
IntActiO95239. 8 interactors.
MINTiMINT-3002743.
STRINGi9606.ENSP00000363524.

Chemistry databases

ChEMBLiCHEMBL6163.

PTM databases

iPTMnetiO95239.
PhosphoSitePlusiO95239.

Polymorphism and mutation databases

BioMutaiKIF4A.

Proteomic databases

EPDiO95239.
MaxQBiO95239.
PaxDbiO95239.
PeptideAtlasiO95239.
PRIDEiO95239.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374403; ENSP00000363524; ENSG00000090889. [O95239-1]
GeneIDi24137.
KEGGihsa:24137.
UCSCiuc004dyg.4. human. [O95239-1]

Organism-specific databases

CTDi24137.
DisGeNETi24137.
GeneCardsiKIF4A.
H-InvDBHIX0016851.
HGNCiHGNC:13339. KIF4A.
HPAiHPA034745.
HPA034746.
MalaCardsiKIF4A.
MIMi300521. gene.
neXtProtiNX_O95239.
OpenTargetsiENSG00000090889.
PharmGKBiPA30105.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOVERGENiHBG052256.
InParanoidiO95239.
KOiK10395.
OMAiRGMEGTT.
OrthoDBiEOG091G02I4.
PhylomeDBiO95239.
TreeFamiTF105224.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000090889-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-437239. Recycling pathway of L1.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.

Miscellaneous databases

ChiTaRSiKIF4A. human.
GeneWikiiKIF4A.
GenomeRNAii24137.
PROiO95239.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000090889.
CleanExiHS_KIF4A.
GenevisibleiO95239. HS.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF4A_HUMAN
AccessioniPrimary (citable) accession number: O95239
Secondary accession number(s): B2R7V5
, D3DVU4, Q86TN3, Q86XX7, Q9NNY6, Q9NY24, Q9UMW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 12, 2005
Last modified: November 30, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.