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O95238

- SPDEF_HUMAN

UniProt

O95238 - SPDEF_HUMAN

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Protein

SAM pointed domain-containing Ets transcription factor

Gene

SPDEF

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi249 – 33284ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome

GO - Biological processi

  1. cell differentiation Source: RefGenome
  2. intestinal epithelial cell development Source: Ensembl
  3. lung goblet cell differentiation Source: Ensembl
  4. multicellular organismal development Source: ProtInc
  5. negative regulation of cell fate commitment Source: Ensembl
  6. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  7. positive regulation of apoptotic process Source: BHF-UCL
  8. positive regulation of cell fate commitment Source: Ensembl
  9. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  10. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SAM pointed domain-containing Ets transcription factor
Alternative name(s):
Prostate epithelium-specific Ets transcription factor
Short name:
Prostate-specific Ets
Prostate-derived Ets factor
Gene namesi
Name:SPDEF
Synonyms:PDEF, PSE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:17257. SPDEF.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134993886.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335SAM pointed domain-containing Ets transcription factorPRO_0000223958Add
BLAST

Proteomic databases

MaxQBiO95238.
PaxDbiO95238.
PRIDEiO95238.

PTM databases

PhosphoSiteiO95238.

Expressioni

Tissue specificityi

Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells.1 Publication

Gene expression databases

BgeeiO95238.
CleanExiHS_SPDEF.
GenevestigatoriO95238.

Organism-specific databases

HPAiHPA055707.

Interactioni

Subunit structurei

Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1.3 Publications

Protein-protein interaction databases

BioGridi117335. 11 interactions.
STRINGi9606.ENSP00000363149.

Structurei

Secondary structure

1
335
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi132 – 1398
Turni140 – 1423
Helixi147 – 1493
Helixi155 – 16511
Helixi172 – 1754
Helixi180 – 1856
Helixi188 – 1947
Beta strandi196 – 1983
Helixi200 – 21314
Helixi251 – 26010
Helixi262 – 2654
Turni266 – 2683
Beta strandi269 – 2735
Turni274 – 2774
Beta strandi278 – 2825
Helixi284 – 29512
Helixi302 – 31110
Turni312 – 3165
Beta strandi317 – 3193
Beta strandi328 – 3325

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YO5X-ray2.00C247-335[»]
2DKXNMR-A131-213[»]
ProteinModelPortaliO95238.
SMRiO95238. Positions 131-213, 247-334.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95238.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 21385PNTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG243414.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000237327.
HOVERGENiHBG080776.
InParanoidiO95238.
KOiK09442.
OMAiMLYPEDS.
OrthoDBiEOG76X60D.
PhylomeDBiO95238.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O95238) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSASPGLSS VSPSHLLLPP DTVSRTGLEK AAAGAVGLER RDWSPSPPAT
60 70 80 90 100
PEQGLSAFYL SYFDMLYPED SSWAAKAPGA SSREEPPEEP EQCPVIDSQA
110 120 130 140 150
PAGSLDLVPG GLTLEEHSLE QVQSMVVGEV LKDIETACKL LNITADPMDW
160 170 180 190 200
SPSNVQKWLL WTEHQYRLPP MGKAFQELAG KELCAMSEEQ FRQRSPLGGD
210 220 230 240 250
VLHAHLDIWK SAAWMKERTS PGAIHYCAST SEESWTDSEV DSSCSGQPIH
260 270 280 290 300
LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGIRKNRPAM
310 320 330
NYDKLSRSIR QYYKKGIIRK PDISQRLVYQ FVHPI
Length:335
Mass (Da):37,518
Last modified:May 1, 1999 - v1
Checksum:iD3117E1AEEBA95EC
GO
Isoform 2 (identifier: O95238-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-227: Missing.

Note: No experimental confirmation available.

Show »
Length:319
Mass (Da):35,715
Checksum:i5C6B944F4885B2B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491A → G in BAG63040. (PubMed:14702039)Curated
Sequence conflicti76 – 761K → N in BAG63040. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti57 – 571A → T.
Corresponds to variant rs2233639 [ dbSNP | Ensembl ].
VAR_048955

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei212 – 22716Missing in isoform 2. 1 PublicationVSP_044722Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB031549 mRNA. Translation: BAA89543.1.
AF071538 mRNA. Translation: AAC95296.1.
AK301543 mRNA. Translation: BAG63040.1.
BX255971, AL157372 Genomic DNA. Translation: CAI23605.1.
BX255972 Genomic DNA. No translation available.
BX255973 Genomic DNA. No translation available.
BC021299 mRNA. Translation: AAH21299.1.
CCDSiCCDS4794.1. [O95238-1]
CCDS59013.1. [O95238-2]
RefSeqiNP_001239223.1. NM_001252294.1. [O95238-2]
NP_036523.1. NM_012391.2. [O95238-1]
XP_005249045.1. XM_005248988.2. [O95238-1]
XP_006715111.1. XM_006715048.1. [O95238-1]
UniGeneiHs.485158.

Genome annotation databases

EnsembliENST00000374037; ENSP00000363149; ENSG00000124664. [O95238-1]
ENST00000544425; ENSP00000442715; ENSG00000124664. [O95238-2]
GeneIDi25803.
KEGGihsa:25803.
UCSCiuc003ojq.2. human. [O95238-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB031549 mRNA. Translation: BAA89543.1 .
AF071538 mRNA. Translation: AAC95296.1 .
AK301543 mRNA. Translation: BAG63040.1 .
BX255971 , AL157372 Genomic DNA. Translation: CAI23605.1 .
BX255972 Genomic DNA. No translation available.
BX255973 Genomic DNA. No translation available.
BC021299 mRNA. Translation: AAH21299.1 .
CCDSi CCDS4794.1. [O95238-1 ]
CCDS59013.1. [O95238-2 ]
RefSeqi NP_001239223.1. NM_001252294.1. [O95238-2 ]
NP_036523.1. NM_012391.2. [O95238-1 ]
XP_005249045.1. XM_005248988.2. [O95238-1 ]
XP_006715111.1. XM_006715048.1. [O95238-1 ]
UniGenei Hs.485158.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1YO5 X-ray 2.00 C 247-335 [» ]
2DKX NMR - A 131-213 [» ]
ProteinModelPortali O95238.
SMRi O95238. Positions 131-213, 247-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 117335. 11 interactions.
STRINGi 9606.ENSP00000363149.

PTM databases

PhosphoSitei O95238.

Proteomic databases

MaxQBi O95238.
PaxDbi O95238.
PRIDEi O95238.

Protocols and materials databases

DNASUi 25803.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000374037 ; ENSP00000363149 ; ENSG00000124664 . [O95238-1 ]
ENST00000544425 ; ENSP00000442715 ; ENSG00000124664 . [O95238-2 ]
GeneIDi 25803.
KEGGi hsa:25803.
UCSCi uc003ojq.2. human. [O95238-1 ]

Organism-specific databases

CTDi 25803.
GeneCardsi GC06M034505.
HGNCi HGNC:17257. SPDEF.
HPAi HPA055707.
MIMi 608144. gene.
neXtProti NX_O95238.
PharmGKBi PA134993886.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG243414.
GeneTreei ENSGT00760000118996.
HOGENOMi HOG000237327.
HOVERGENi HBG080776.
InParanoidi O95238.
KOi K09442.
OMAi MLYPEDS.
OrthoDBi EOG76X60D.
PhylomeDBi O95238.
TreeFami TF318679.

Miscellaneous databases

EvolutionaryTracei O95238.
GeneWikii SPDEF.
GenomeRNAii 25803.
NextBioi 47011.
PROi O95238.
SOURCEi Search...

Gene expression databases

Bgeei O95238.
CleanExi HS_SPDEF.
Genevestigatori O95238.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProi IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view ]
PRINTSi PR00454. ETSDOMAIN.
SMARTi SM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the mouse Pse gene encoding a novel Ets family member."
    Yamada N., Tamai Y., Miyamoto H., Nozaki M.
    Gene 241:267-274(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Prostatic carcinoma.
  2. "PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression."
    Oettgen P., Finger E., Sun Z., Akbarali Y., Thamrongsak U., Boltax J., Grall F., Dube A., Weiss A., Brown L., Quinn G., Kas K., Endress G., Kunsch C., Libermann T.A.
    J. Biol. Chem. 275:1216-1225(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH AR.
    Tissue: Prostate.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary gland.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon.
  6. "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter."
    Chen H., Nandi A.K., Li X., Bieberich C.J.
    Cancer Res. 62:338-340(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NKX3-1.
  7. "Analysis of the 2.0 A crystal structure of the protein-DNA complex of the human PDEF Ets domain bound to the prostate specific antigen regulatory site."
    Wang Y., Feng L., Said M., Balderman S., Fayazi Z., Liu Y., Ghosh D., Gulick A.M.
    Biochemistry 44:7095-7106(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 247-335 IN COMPLEX WITH DNA.
  8. "Solution structure of the SAM_pnt-domain of ETS transcription factor PDEF (prostate Ets)."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 131-213.

Entry informationi

Entry nameiSPDEF_HUMAN
AccessioniPrimary (citable) accession number: O95238
Secondary accession number(s): B4DWH8, F5H778
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3