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Protein

SAM pointed domain-containing Ets transcription factor

Gene

SPDEF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi249 – 332ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124664-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SAM pointed domain-containing Ets transcription factor
Alternative name(s):
Prostate epithelium-specific Ets transcription factor
Short name:
Prostate-specific Ets
Prostate-derived Ets factor
Gene namesi
Name:SPDEF
Synonyms:PDEF, PSE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:17257. SPDEF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi25803.
OpenTargetsiENSG00000124664.
PharmGKBiPA134993886.

Polymorphism and mutation databases

BioMutaiSPDEF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002239581 – 335SAM pointed domain-containing Ets transcription factorAdd BLAST335

Proteomic databases

EPDiO95238.
MaxQBiO95238.
PaxDbiO95238.
PeptideAtlasiO95238.
PRIDEiO95238.

PTM databases

iPTMnetiO95238.
PhosphoSitePlusiO95238.

Expressioni

Tissue specificityi

Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells.1 Publication

Gene expression databases

BgeeiENSG00000124664.
CleanExiHS_SPDEF.
GenevisibleiO95238. HS.

Organism-specific databases

HPAiHPA055707.

Interactioni

Subunit structurei

Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1.3 Publications

Protein-protein interaction databases

BioGridi117335. 14 interactors.
STRINGi9606.ENSP00000363149.

Structurei

Secondary structure

1335
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi132 – 139Combined sources8
Turni140 – 142Combined sources3
Helixi147 – 149Combined sources3
Helixi155 – 165Combined sources11
Helixi172 – 175Combined sources4
Helixi180 – 185Combined sources6
Helixi188 – 194Combined sources7
Beta strandi196 – 198Combined sources3
Helixi200 – 213Combined sources14
Helixi251 – 260Combined sources10
Helixi262 – 265Combined sources4
Turni266 – 268Combined sources3
Beta strandi269 – 273Combined sources5
Turni274 – 277Combined sources4
Beta strandi278 – 282Combined sources5
Helixi284 – 295Combined sources12
Helixi302 – 311Combined sources10
Turni312 – 316Combined sources5
Beta strandi317 – 319Combined sources3
Beta strandi328 – 332Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YO5X-ray2.00C247-335[»]
2DKXNMR-A131-213[»]
ProteinModelPortaliO95238.
SMRiO95238.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95238.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 213PNTPROSITE-ProRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3805. Eukaryota.
ENOG410XSXU. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000237327.
HOVERGENiHBG080776.
InParanoidiO95238.
KOiK09442.
OMAiYPEDSSW.
OrthoDBiEOG091G07NK.
PhylomeDBiO95238.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95238-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSASPGLSS VSPSHLLLPP DTVSRTGLEK AAAGAVGLER RDWSPSPPAT
60 70 80 90 100
PEQGLSAFYL SYFDMLYPED SSWAAKAPGA SSREEPPEEP EQCPVIDSQA
110 120 130 140 150
PAGSLDLVPG GLTLEEHSLE QVQSMVVGEV LKDIETACKL LNITADPMDW
160 170 180 190 200
SPSNVQKWLL WTEHQYRLPP MGKAFQELAG KELCAMSEEQ FRQRSPLGGD
210 220 230 240 250
VLHAHLDIWK SAAWMKERTS PGAIHYCAST SEESWTDSEV DSSCSGQPIH
260 270 280 290 300
LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGIRKNRPAM
310 320 330
NYDKLSRSIR QYYKKGIIRK PDISQRLVYQ FVHPI
Length:335
Mass (Da):37,518
Last modified:May 1, 1999 - v1
Checksum:iD3117E1AEEBA95EC
GO
Isoform 2 (identifier: O95238-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-227: Missing.

Note: No experimental confirmation available.
Show »
Length:319
Mass (Da):35,715
Checksum:i5C6B944F4885B2B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49A → G in BAG63040 (PubMed:14702039).Curated1
Sequence conflicti76K → N in BAG63040 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04895557A → T.Corresponds to variant rs2233639dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044722212 – 227Missing in isoform 2. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031549 mRNA. Translation: BAA89543.1.
AF071538 mRNA. Translation: AAC95296.1.
AK301543 mRNA. Translation: BAG63040.1.
BX255971, AL157372 Genomic DNA. Translation: CAI23605.1.
BX255972 Genomic DNA. No translation available.
BX255973 Genomic DNA. No translation available.
BC021299 mRNA. Translation: AAH21299.1.
CCDSiCCDS4794.1. [O95238-1]
CCDS59013.1. [O95238-2]
RefSeqiNP_001239223.1. NM_001252294.1. [O95238-2]
NP_036523.1. NM_012391.2. [O95238-1]
UniGeneiHs.485158.

Genome annotation databases

EnsembliENST00000374037; ENSP00000363149; ENSG00000124664. [O95238-1]
ENST00000544425; ENSP00000442715; ENSG00000124664. [O95238-2]
GeneIDi25803.
KEGGihsa:25803.
UCSCiuc003ojq.3. human. [O95238-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031549 mRNA. Translation: BAA89543.1.
AF071538 mRNA. Translation: AAC95296.1.
AK301543 mRNA. Translation: BAG63040.1.
BX255971, AL157372 Genomic DNA. Translation: CAI23605.1.
BX255972 Genomic DNA. No translation available.
BX255973 Genomic DNA. No translation available.
BC021299 mRNA. Translation: AAH21299.1.
CCDSiCCDS4794.1. [O95238-1]
CCDS59013.1. [O95238-2]
RefSeqiNP_001239223.1. NM_001252294.1. [O95238-2]
NP_036523.1. NM_012391.2. [O95238-1]
UniGeneiHs.485158.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YO5X-ray2.00C247-335[»]
2DKXNMR-A131-213[»]
ProteinModelPortaliO95238.
SMRiO95238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117335. 14 interactors.
STRINGi9606.ENSP00000363149.

PTM databases

iPTMnetiO95238.
PhosphoSitePlusiO95238.

Polymorphism and mutation databases

BioMutaiSPDEF.

Proteomic databases

EPDiO95238.
MaxQBiO95238.
PaxDbiO95238.
PeptideAtlasiO95238.
PRIDEiO95238.

Protocols and materials databases

DNASUi25803.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374037; ENSP00000363149; ENSG00000124664. [O95238-1]
ENST00000544425; ENSP00000442715; ENSG00000124664. [O95238-2]
GeneIDi25803.
KEGGihsa:25803.
UCSCiuc003ojq.3. human. [O95238-1]

Organism-specific databases

CTDi25803.
DisGeNETi25803.
GeneCardsiSPDEF.
HGNCiHGNC:17257. SPDEF.
HPAiHPA055707.
MIMi608144. gene.
neXtProtiNX_O95238.
OpenTargetsiENSG00000124664.
PharmGKBiPA134993886.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3805. Eukaryota.
ENOG410XSXU. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000237327.
HOVERGENiHBG080776.
InParanoidiO95238.
KOiK09442.
OMAiYPEDSSW.
OrthoDBiEOG091G07NK.
PhylomeDBiO95238.
TreeFamiTF318679.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124664-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO95238.
GeneWikiiSPDEF.
GenomeRNAii25803.
PROiO95238.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124664.
CleanExiHS_SPDEF.
GenevisibleiO95238. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPDEF_HUMAN
AccessioniPrimary (citable) accession number: O95238
Secondary accession number(s): B4DWH8, F5H778
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.