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Protein

SAM pointed domain-containing Ets transcription factor

Gene

SPDEF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi249 – 332ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SIGNORiO95238

Names & Taxonomyi

Protein namesi
Recommended name:
SAM pointed domain-containing Ets transcription factor
Alternative name(s):
Prostate epithelium-specific Ets transcription factor
Short name:
Prostate-specific Ets
Prostate-derived Ets factor
Gene namesi
Name:SPDEF
Synonyms:PDEF, PSE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000124664.10
HGNCiHGNC:17257 SPDEF
MIMi608144 gene
neXtProtiNX_O95238

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi25803
OpenTargetsiENSG00000124664
PharmGKBiPA134993886

Polymorphism and mutation databases

BioMutaiSPDEF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002239581 – 335SAM pointed domain-containing Ets transcription factorAdd BLAST335

Proteomic databases

EPDiO95238
MaxQBiO95238
PaxDbiO95238
PeptideAtlasiO95238
PRIDEiO95238
ProteomicsDBi50737

PTM databases

iPTMnetiO95238
PhosphoSitePlusiO95238

Expressioni

Tissue specificityi

Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells.1 Publication

Gene expression databases

BgeeiENSG00000124664
CleanExiHS_SPDEF
GenevisibleiO95238 HS

Organism-specific databases

HPAiHPA055707

Interactioni

Subunit structurei

Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1.3 Publications

Protein-protein interaction databases

BioGridi117335, 14 interactors
IntActiO95238, 7 interactors
STRINGi9606.ENSP00000363149

Structurei

Secondary structure

1335
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi132 – 139Combined sources8
Turni140 – 142Combined sources3
Helixi147 – 149Combined sources3
Helixi155 – 165Combined sources11
Helixi172 – 175Combined sources4
Helixi180 – 185Combined sources6
Helixi188 – 194Combined sources7
Beta strandi196 – 198Combined sources3
Helixi200 – 213Combined sources14
Helixi251 – 260Combined sources10
Helixi262 – 265Combined sources4
Turni266 – 268Combined sources3
Beta strandi269 – 273Combined sources5
Turni274 – 277Combined sources4
Beta strandi278 – 282Combined sources5
Helixi284 – 295Combined sources12
Helixi302 – 311Combined sources10
Turni312 – 316Combined sources5
Beta strandi317 – 319Combined sources3
Beta strandi328 – 332Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YO5X-ray2.00C247-335[»]
2DKXNMR-A131-213[»]
ProteinModelPortaliO95238
SMRiO95238
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95238

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 213PNTPROSITE-ProRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00760000118996
HOGENOMiHOG000237327
HOVERGENiHBG080776
InParanoidiO95238
KOiK09442
OMAiYPEDSSW
OrthoDBiEOG091G07NK
PhylomeDBiO95238
TreeFamiTF318679

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR003118 Pointed_dom
IPR013761 SAM/pointed_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PF02198 SAM_PNT, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SM00251 SAM_PNT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit
PS51433 PNT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95238-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSASPGLSS VSPSHLLLPP DTVSRTGLEK AAAGAVGLER RDWSPSPPAT
60 70 80 90 100
PEQGLSAFYL SYFDMLYPED SSWAAKAPGA SSREEPPEEP EQCPVIDSQA
110 120 130 140 150
PAGSLDLVPG GLTLEEHSLE QVQSMVVGEV LKDIETACKL LNITADPMDW
160 170 180 190 200
SPSNVQKWLL WTEHQYRLPP MGKAFQELAG KELCAMSEEQ FRQRSPLGGD
210 220 230 240 250
VLHAHLDIWK SAAWMKERTS PGAIHYCAST SEESWTDSEV DSSCSGQPIH
260 270 280 290 300
LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGIRKNRPAM
310 320 330
NYDKLSRSIR QYYKKGIIRK PDISQRLVYQ FVHPI
Length:335
Mass (Da):37,518
Last modified:May 1, 1999 - v1
Checksum:iD3117E1AEEBA95EC
GO
Isoform 2 (identifier: O95238-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-227: Missing.

Note: No experimental confirmation available.
Show »
Length:319
Mass (Da):35,715
Checksum:i5C6B944F4885B2B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49A → G in BAG63040 (PubMed:14702039).Curated1
Sequence conflicti76K → N in BAG63040 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04895557A → T. Corresponds to variant dbSNP:rs2233639Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044722212 – 227Missing in isoform 2. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031549 mRNA Translation: BAA89543.1
AF071538 mRNA Translation: AAC95296.1
AK301543 mRNA Translation: BAG63040.1
BX255971, AL157372 Genomic DNA Translation: CAI23605.1
BX255972 Genomic DNA No translation available.
BX255973 Genomic DNA No translation available.
BC021299 mRNA Translation: AAH21299.1
CCDSiCCDS4794.1 [O95238-1]
CCDS59013.1 [O95238-2]
RefSeqiNP_001239223.1, NM_001252294.1 [O95238-2]
NP_036523.1, NM_012391.2 [O95238-1]
UniGeneiHs.485158

Genome annotation databases

EnsembliENST00000374037; ENSP00000363149; ENSG00000124664 [O95238-1]
ENST00000544425; ENSP00000442715; ENSG00000124664 [O95238-2]
GeneIDi25803
KEGGihsa:25803
UCSCiuc003ojq.3 human [O95238-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSPDEF_HUMAN
AccessioniPrimary (citable) accession number: O95238
Secondary accession number(s): B4DWH8, F5H778
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: May 1, 1999
Last modified: June 20, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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