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Protein

Kinesin-like protein KIF20A

Gene

KIF20A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi160 – 167ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • microtubule binding Source: InterPro
  • microtubule motor activity Source: GO_Central
  • protein kinase binding Source: UniProtKB
  • transporter activity Source: ProtInc

GO - Biological processi

  • cell separation after cytokinesis Source: UniProtKB
  • microtubule-based movement Source: GO_Central
  • microtubule bundle formation Source: UniProtKB
  • mitotic cytokinesis Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • regulation of cytokinesis Source: UniProtKB
  • vesicle-mediated transport Source: ProtInc

Keywordsi

Molecular functionMotor protein
Biological processProtein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2132295 MHC class II antigen presentation
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-68884 Mitotic Telophase/Cytokinesis
R-HSA-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF20A
Alternative name(s):
GG10_2
Mitotic kinesin-like protein 2
Short name:
MKlp2
Rab6-interacting kinesin-like protein
Rabkinesin-6
Gene namesi
Name:KIF20A
Synonyms:MKLP2, RAB6KIFL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000112984.11
HGNCiHGNC:9787 KIF20A
MIMi605664 gene
neXtProtiNX_O95235

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi528S → A: Impairs phosphorylation by PLK1 and recruitment of PLK1 to the spindle. 1 Publication1

Organism-specific databases

DisGeNETi10112
OpenTargetsiENSG00000112984
PharmGKBiPA34149

Chemistry databases

ChEMBLiCHEMBL2021753

Polymorphism and mutation databases

BioMutaiKIF20A

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001254602 – 890Kinesin-like protein KIF20AAdd BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Modified residuei528Phosphoserine; by PLK1Combined sources1 Publication1
Modified residuei532PhosphoserineCombined sources1
Modified residuei662Phosphoserine1 Publication1
Modified residuei668Phosphoserine1 Publication1
Modified residuei685PhosphoserineCombined sources1
Modified residuei825PhosphoserineCombined sources1
Modified residuei857PhosphothreonineCombined sources1
Modified residuei867PhosphoserineCombined sources1
Modified residuei878PhosphoserineCombined sources1
Modified residuei883PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO95235
MaxQBiO95235
PaxDbiO95235
PeptideAtlasiO95235
PRIDEiO95235
ProteomicsDBi50732

PTM databases

iPTMnetiO95235
PhosphoSitePlusiO95235

Expressioni

Gene expression databases

BgeeiENSG00000112984
CleanExiHS_KIF20A
ExpressionAtlasiO95235 baseline and differential
GenevisibleiO95235 HS

Organism-specific databases

HPAiHPA036909
HPA036910

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FAM214BQ7L5A33EBI-2551319,EBI-745689

GO - Molecular functioni

  • microtubule binding Source: InterPro
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115418, 46 interactors
IntActiO95235, 38 interactors
MINTiO95235
STRINGi9606.ENSP00000378356

Chemistry databases

BindingDBiO95235

Structurei

3D structure databases

ProteinModelPortaliO95235
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 507Kinesin motorPROSITE-ProRule annotationAdd BLAST444

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni763 – 890GlobularSequence analysisAdd BLAST128

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili611 – 762Sequence analysisAdd BLAST152

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0247 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00920000148953
HOGENOMiHOG000054207
HOVERGENiHBG052246
InParanoidiO95235
KOiK10402
OMAiRLCEDQN
OrthoDBiEOG091G00AY
PhylomeDBiO95235
TreeFamiTF105232

Family and domain databases

Gene3Di3.40.850.10, 2 hits
InterProiView protein in InterPro
IPR027326 KIF20A
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PTHR24115:SF352 PTHR24115:SF352, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95235-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQGILSPPA GLLSDDDVVV SPMFESTAAD LGSVVRKNLL SDCSVVSTSL
60 70 80 90 100
EDKQQVPSED SMEKVKVYLR VRPLLPSELE RQEDQGCVRI ENVETLVLQA
110 120 130 140 150
PKDSFALKSN ERGIGQATHR FTFSQIFGPE VGQASFFNLT VKEMVKDVLK
160 170 180 190 200
GQNWLIYTYG VTNSGKTHTI QGTIKDGGIL PRSLALIFNS LQGQLHPTPD
210 220 230 240 250
LKPLLSNEVI WLDSKQIRQE EMKKLSLLNG GLQEEELSTS LKRSVYIESR
260 270 280 290 300
IGTSTSFDSG IAGLSSISQC TSSSQLDETS HRWAQPDTAP LPVPANIRFS
310 320 330 340 350
IWISFFEIYN ELLYDLLEPP SQQRKRQTLR LCEDQNGNPY VKDLNWIHVQ
360 370 380 390 400
DAEEAWKLLK VGRKNQSFAS THLNQNSSRS HSIFSIRILH LQGEGDIVPK
410 420 430 440 450
ISELSLCDLA GSERCKDQKS GERLKEAGNI NTSLHTLGRC IAALRQNQQN
460 470 480 490 500
RSKQNLVPFR DSKLTRVFQG FFTGRGRSCM IVNVNPCAST YDETLHVAKF
510 520 530 540 550
SAIASQLVHA PPMQLGFPSL HSFIKEHSLQ VSPSLEKGAK ADTGLDDDIE
560 570 580 590 600
NEADISMYGK EELLQVVEAM KTLLLKERQE KLQLEMHLRD EICNEMVEQM
610 620 630 640 650
QQREQWCSEH LDTQKELLEE MYEEKLNILK ESLTSFYQEE IQERDEKIEE
660 670 680 690 700
LEALLQEARQ QSVAHQQSGS ELALRRSQRL AASASTQQLQ EVKAKLQQCK
710 720 730 740 750
AELNSTTEEL HKYQKMLEPP PSAKPFTIDV DKKLEEGQKN IRLLRTELQK
760 770 780 790 800
LGESLQSAER ACCHSTGAGK LRQALTTCDD ILIKQDQTLA ELQNNMVLVK
810 820 830 840 850
LDLRKKAACI AEQYHTVLKL QGQVSAKKRL GTNQENQQPN QQPPGKKPFL
860 870 880 890
RNLLPRTPTC QSSTDCSPYA RILRSRRSPL LKSGPFGKKY
Length:890
Mass (Da):100,278
Last modified:May 1, 1999 - v1
Checksum:i6620264615496051
GO
Isoform 2 (identifier: O95235-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-82: Missing.

Note: No experimental confirmation available.
Show »
Length:872
Mass (Da):98,100
Checksum:i3E7BB0907A62A23B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04970463E → K. Corresponds to variant dbSNP:rs3734116Ensembl.1
Natural variantiVAR_049705839P → L. Corresponds to variant dbSNP:rs3172747Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05600765 – 82Missing in isoform 2. 1 PublicationAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070672 mRNA Translation: AAC83230.1
AF153329 mRNA Translation: AAD37806.1
AK296879 mRNA Translation: BAG59441.1
AC106752 Genomic DNA No translation available.
AC109442 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW62157.1
CH471062 Genomic DNA Translation: EAW62158.1
BC012999 mRNA Translation: AAH12999.1
CCDSiCCDS4199.1 [O95235-1]
RefSeqiNP_005724.1, NM_005733.2 [O95235-1]
UniGeneiHs.718626

Genome annotation databases

EnsembliENST00000394894; ENSP00000378356; ENSG00000112984 [O95235-1]
ENST00000508792; ENSP00000420880; ENSG00000112984 [O95235-2]
GeneIDi10112
KEGGihsa:10112
UCSCiuc003lcj.4 human [O95235-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKI20A_HUMAN
AccessioniPrimary (citable) accession number: O95235
Secondary accession number(s): B4DL79, D3DQB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: June 20, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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