O95235 (KI20A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIF20A

Alternative name(s):
GG10_2
Mitotic kinesin-like protein 2
Short name=MKlp2
Rab6-interacting kinesin-like protein
Rabkinesin-6

Gene names

Name:	KIF20A
Synonyms:	MKLP2, RAB6KIFL

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. Ref.5
Subcellular location	Golgi apparatus By similarity. Cytoplasm › cytoskeleton › spindle Ref.5.
Post-translational modification	Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Sequence similarities	Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Microtubule
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	M phase of mitotic cell cycle Traceable author statement. Source: Reactome cytokinesis Traceable author statement. Source: Reactome microtubule-based movement Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle-mediated transport Traceable author statement. Source: ProtInc
Cellular component	Golgi apparatus Traceable author statement. Source: ProtInc microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleoplasm Traceable author statement. Source: Reactome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Inferred from electronic annotation. Source: InterPro transporter activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 890	890	Kinesin-like protein KIF20A		PRO_0000125460
Regions
	Domain	70 – 495	426	Kinesin-motor
	Nucleotide binding	160 – 167	8	ATP By similarity
	Region	763 – 890	128	Globular Potential
	Coiled coil	611 – 762	152	Potential
Amino acid modifications
	Modified residue	21	1	Phosphoserine Ref.8
	Modified residue	49	1	Phosphoserine Ref.7
	Modified residue	238	1	Phosphoserine Ref.8
	Modified residue	244	1	Phosphoserine Ref.7 Ref.8
	Modified residue	246	1	Phosphotyrosine Ref.8
	Modified residue	249	1	Phosphoserine Ref.8
	Modified residue	256	1	Phosphoserine Ref.11
	Modified residue	528	1	Phosphoserine; by PLK1 Ref.5 Ref.7 Ref.8 Ref.11
	Modified residue	532	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.10 Ref.11
	Modified residue	556	1	Phosphoserine Ref.7 Ref.8
	Modified residue	662	1	Phosphoserine Ref.5 Ref.10
	Modified residue	668	1	Phosphoserine
	Modified residue	670	1	Phosphoserine Ref.8
	Modified residue	683	1	Phosphoserine Ref.8
	Modified residue	825	1	Phosphoserine Ref.8
	Modified residue	857	1	Phosphothreonine Ref.9 Ref.11
	Modified residue	859	1	Phosphothreonine Ref.9 Ref.11
	Modified residue	867	1	Phosphoserine Ref.7 Ref.8 Ref.11 Ref.12
Natural variations
	Natural variant	63	1	E → K. Corresponds to variant rs3734116 [ dbSNP | Ensembl ].		VAR_049704
	Natural variant	839	1	P → L. Corresponds to variant rs3172747 [ dbSNP | Ensembl ].		VAR_049705
Experimental info
	Mutagenesis	528	1	S → A: Impairs phosphorylation by PLK1 and recruitment of PLK1 to the spindle. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O95235 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6620264615496051	FASTA	890	100,278
	10 20 30 40 50 60 MSQGILSPPA GLLSDDDVVV SPMFESTAAD LGSVVRKNLL SDCSVVSTSL EDKQQVPSED 70 80 90 100 110 120 SMEKVKVYLR VRPLLPSELE RQEDQGCVRI ENVETLVLQA PKDSFALKSN ERGIGQATHR 130 140 150 160 170 180 FTFSQIFGPE VGQASFFNLT VKEMVKDVLK GQNWLIYTYG VTNSGKTHTI QGTIKDGGIL 190 200 210 220 230 240 PRSLALIFNS LQGQLHPTPD LKPLLSNEVI WLDSKQIRQE EMKKLSLLNG GLQEEELSTS 250 260 270 280 290 300 LKRSVYIESR IGTSTSFDSG IAGLSSISQC TSSSQLDETS HRWAQPDTAP LPVPANIRFS 310 320 330 340 350 360 IWISFFEIYN ELLYDLLEPP SQQRKRQTLR LCEDQNGNPY VKDLNWIHVQ DAEEAWKLLK 370 380 390 400 410 420 VGRKNQSFAS THLNQNSSRS HSIFSIRILH LQGEGDIVPK ISELSLCDLA GSERCKDQKS 430 440 450 460 470 480 GERLKEAGNI NTSLHTLGRC IAALRQNQQN RSKQNLVPFR DSKLTRVFQG FFTGRGRSCM 490 500 510 520 530 540 IVNVNPCAST YDETLHVAKF SAIASQLVHA PPMQLGFPSL HSFIKEHSLQ VSPSLEKGAK 550 560 570 580 590 600 ADTGLDDDIE NEADISMYGK EELLQVVEAM KTLLLKERQE KLQLEMHLRD EICNEMVEQM 610 620 630 640 650 660 QQREQWCSEH LDTQKELLEE MYEEKLNILK ESLTSFYQEE IQERDEKIEE LEALLQEARQ 670 680 690 700 710 720 QSVAHQQSGS ELALRRSQRL AASASTQQLQ EVKAKLQQCK AELNSTTEEL HKYQKMLEPP 730 740 750 760 770 780 PSAKPFTIDV DKKLEEGQKN IRLLRTELQK LGESLQSAER ACCHSTGAGK LRQALTTCDD 790 800 810 820 830 840 ILIKQDQTLA ELQNNMVLVK LDLRKKAACI AEQYHTVLKL QGQVSAKKRL GTNQENQQPN 850 860 870 880 890 QQPPGKKPFL RNLLPRTPTC QSSTDCSPYA RILRSRRSPL LKSGPFGKKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vascular endothelial genes that are responsive to tumor necrosis factor-alpha in vitro are expressed in atherosclerotic lesions, including inhibitor of apoptosis protein-1, stannin, and two novel genes." Horrevoets A.J.G., Fontijn R.D., van Zonneveld A.J., de Vries C.J.M., ten Cate J.W., Pannekoek H. Blood 93:3418-3431(1999) [PubMed: 10233894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Endothelial cell.
[2]	"cDNA cloning, expression pattern, genomic structure and chromosomal location of RAB6KIFL, a human kinesin-like gene." Lai F., Fernald A.A., Zhao N., Le Beau M.M. Gene 248:117-125(2000) [PubMed: 10806357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[5]	"Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis." Neef R., Preisinger C., Sutcliffe J., Kopajtich R., Nigg E.A., Mayer T.U., Barr F.A. J. Cell Biol. 162:863-875(2003) [PubMed: 12939256] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PLK1, PHOSPHORYLATION AT SER-528 AND SER-662, MUTAGENESIS OF SER-528, FUNCTION.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; SER-244; SER-528; SER-532; SER-556 AND SER-867, MASS SPECTROMETRY. Tissue: Cervix adenocarcinoma.
[8]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-238; SER-244; TYR-246; SER-249; SER-528; SER-532; SER-556; SER-670; SER-683; SER-825 AND SER-867, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-857 AND THR-859, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532 AND SER-662, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-256; SER-528; SER-532; THR-857; THR-859 AND SER-867, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-867, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF070672 mRNA. Translation: AAC83230.1. AF153329 mRNA. Translation: AAD37806.1. CH471062 Genomic DNA. Translation: EAW62157.1. CH471062 Genomic DNA. Translation: EAW62158.1. BC012999 mRNA. Translation: AAH12999.1.
IPI	IPI00029422.
RefSeq	NP_005724.1. NM_005733.2.
UniGene	Hs.718626.
3D structure databases
ProteinModelPortal	O95235.
SMR	O95235. Positions 61-517.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95235. 3 interactions.
STRING	O95235.
PTM databases
PhosphoSite	O95235.
Proteomic databases
PeptideAtlas	O95235.
PRIDE	O95235.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000394894; ENSP00000378356; ENSG00000112984.
GeneID	10112.
KEGG	hsa:10112.
UCSC	uc003lcj.1. human.
Organism-specific databases
CTD	10112.
GeneCards	GC05P137542.
H-InvDB	HIX0005207.
HGNC	HGNC:9787. KIF20A.
HPA	HPA036909. HPA036910.
MIM	605664. gene.
neXtProt	NX_O95235.
PharmGKB	PA34149.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18229.
GeneTree	ENSGT00530000063172.
HOGENOM	HBG717776.
HOVERGEN	HBG052246.
InParanoid	O95235.
OMA	VPADIRF.
OrthoDB	EOG4VT5WT.
PhylomeDB	O95235.
Enzyme and pathway databases
Pathway_Interaction_DB	aurora_b_pathway. Aurora B signaling.
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_383. DNA Replication. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	O95235.
Bgee	O95235.
CleanEx	HS_KIF20A.
Genevestigator	O95235.
GermOnline	ENSG00000112984. Homo sapiens.
Family and domain databases
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Gene3D	G3DSA:3.40.850.10. kinesin_motor. 2 hits.
KO	K10402.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38255.
SOURCE	Search...

Entry information

Entry name

KI20A_HUMAN

Accession

Primary (citable) accession number: O95235
Secondary accession number(s): D3DQB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.