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O95235

- KI20A_HUMAN

UniProt

O95235 - KI20A_HUMAN

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Protein
Kinesin-like protein KIF20A
Gene
KIF20A, MKLP2, RAB6KIFL
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1678ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. microtubule motor activity Source: InterPro
  3. protein kinase binding Source: UniProtKB
  4. transporter activity Source: ProtInc
Complete GO annotation...

GO - Biological processi

  1. cytokinesis Source: UniProtKB
  2. microtubule bundle formation Source: UniProtKB
  3. microtubule-based movement Source: InterPro
  4. mitotic cell cycle Source: Reactome
  5. protein transport Source: UniProtKB-KW
  6. vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_121399. MHC class II antigen presentation.
REACT_1932. Mitotic Telophase/Cytokinesis.
REACT_25201. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF20A
Alternative name(s):
GG10_2
Mitotic kinesin-like protein 2
Short name:
MKlp2
Rab6-interacting kinesin-like protein
Rabkinesin-6
Gene namesi
Name:KIF20A
Synonyms:MKLP2, RAB6KIFL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:9787. KIF20A.

Subcellular locationi

Golgi apparatus By similarity. Cytoplasmcytoskeletonspindle 1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: ProtInc
  2. kinesin complex Source: InterPro
  3. microtubule Source: UniProtKB-KW
  4. nucleoplasm Source: Reactome
  5. spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi528 – 5281S → A: Impairs phosphorylation by PLK1 and recruitment of PLK1 to the spindle. 1 Publication

Organism-specific databases

PharmGKBiPA34149.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 890889Kinesin-like protein KIF20A
PRO_0000125460Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei7 – 71Phosphoserine1 Publication
Modified residuei14 – 141Phosphoserine1 Publication
Modified residuei21 – 211Phosphoserine1 Publication
Modified residuei528 – 5281Phosphoserine; by PLK12 Publications
Modified residuei532 – 5321Phosphoserine3 Publications
Modified residuei662 – 6621Phosphoserine1 Publication
Modified residuei668 – 6681Phosphoserine1 Publication
Modified residuei825 – 8251Phosphoserine1 Publication
Modified residuei857 – 8571Phosphothreonine1 Publication
Modified residuei867 – 8671Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO95235.
PaxDbiO95235.
PeptideAtlasiO95235.
PRIDEiO95235.

PTM databases

PhosphoSiteiO95235.

Expressioni

Gene expression databases

ArrayExpressiO95235.
BgeeiO95235.
CleanExiHS_KIF20A.
GenevestigatoriO95235.

Organism-specific databases

HPAiHPA036909.
HPA036910.

Interactioni

Protein-protein interaction databases

BioGridi115418. 11 interactions.
IntActiO95235. 4 interactions.
MINTiMINT-4989469.
STRINGi9606.ENSP00000378356.

Structurei

3D structure databases

ProteinModelPortaliO95235.
SMRiO95235. Positions 56-516.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 507444Kinesin motor
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni763 – 890128Globular Reviewed prediction
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili611 – 762152 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
HOGENOMiHOG000054207.
HOVERGENiHBG052246.
InParanoidiO95235.
KOiK10402.
OMAiRLCEDQN.
OrthoDBiEOG74BJR9.
PhylomeDBiO95235.
TreeFamiTF105232.

Family and domain databases

Gene3Di3.40.850.10. 2 hits.
InterProiIPR027326. KIF20A.
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PTHR24115:SF352. PTHR24115:SF352. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95235-1 [UniParc]FASTAAdd to Basket

« Hide

MSQGILSPPA GLLSDDDVVV SPMFESTAAD LGSVVRKNLL SDCSVVSTSL    50
EDKQQVPSED SMEKVKVYLR VRPLLPSELE RQEDQGCVRI ENVETLVLQA 100
PKDSFALKSN ERGIGQATHR FTFSQIFGPE VGQASFFNLT VKEMVKDVLK 150
GQNWLIYTYG VTNSGKTHTI QGTIKDGGIL PRSLALIFNS LQGQLHPTPD 200
LKPLLSNEVI WLDSKQIRQE EMKKLSLLNG GLQEEELSTS LKRSVYIESR 250
IGTSTSFDSG IAGLSSISQC TSSSQLDETS HRWAQPDTAP LPVPANIRFS 300
IWISFFEIYN ELLYDLLEPP SQQRKRQTLR LCEDQNGNPY VKDLNWIHVQ 350
DAEEAWKLLK VGRKNQSFAS THLNQNSSRS HSIFSIRILH LQGEGDIVPK 400
ISELSLCDLA GSERCKDQKS GERLKEAGNI NTSLHTLGRC IAALRQNQQN 450
RSKQNLVPFR DSKLTRVFQG FFTGRGRSCM IVNVNPCAST YDETLHVAKF 500
SAIASQLVHA PPMQLGFPSL HSFIKEHSLQ VSPSLEKGAK ADTGLDDDIE 550
NEADISMYGK EELLQVVEAM KTLLLKERQE KLQLEMHLRD EICNEMVEQM 600
QQREQWCSEH LDTQKELLEE MYEEKLNILK ESLTSFYQEE IQERDEKIEE 650
LEALLQEARQ QSVAHQQSGS ELALRRSQRL AASASTQQLQ EVKAKLQQCK 700
AELNSTTEEL HKYQKMLEPP PSAKPFTIDV DKKLEEGQKN IRLLRTELQK 750
LGESLQSAER ACCHSTGAGK LRQALTTCDD ILIKQDQTLA ELQNNMVLVK 800
LDLRKKAACI AEQYHTVLKL QGQVSAKKRL GTNQENQQPN QQPPGKKPFL 850
RNLLPRTPTC QSSTDCSPYA RILRSRRSPL LKSGPFGKKY 890
Length:890
Mass (Da):100,278
Last modified:May 1, 1999 - v1
Checksum:i6620264615496051
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti63 – 631E → K.
Corresponds to variant rs3734116 [ dbSNP | Ensembl ].
VAR_049704
Natural varianti839 – 8391P → L.
Corresponds to variant rs3172747 [ dbSNP | Ensembl ].
VAR_049705

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF070672 mRNA. Translation: AAC83230.1.
AF153329 mRNA. Translation: AAD37806.1.
CH471062 Genomic DNA. Translation: EAW62157.1.
CH471062 Genomic DNA. Translation: EAW62158.1.
BC012999 mRNA. Translation: AAH12999.1.
CCDSiCCDS4199.1.
RefSeqiNP_005724.1. NM_005733.2.
XP_006714577.1. XM_006714514.1.
UniGeneiHs.718626.

Genome annotation databases

EnsembliENST00000394894; ENSP00000378356; ENSG00000112984.
GeneIDi10112.
KEGGihsa:10112.
UCSCiuc003lcj.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF070672 mRNA. Translation: AAC83230.1 .
AF153329 mRNA. Translation: AAD37806.1 .
CH471062 Genomic DNA. Translation: EAW62157.1 .
CH471062 Genomic DNA. Translation: EAW62158.1 .
BC012999 mRNA. Translation: AAH12999.1 .
CCDSi CCDS4199.1.
RefSeqi NP_005724.1. NM_005733.2.
XP_006714577.1. XM_006714514.1.
UniGenei Hs.718626.

3D structure databases

ProteinModelPortali O95235.
SMRi O95235. Positions 56-516.
ModBasei Search...

Protein-protein interaction databases

BioGridi 115418. 11 interactions.
IntActi O95235. 4 interactions.
MINTi MINT-4989469.
STRINGi 9606.ENSP00000378356.

Chemistry

ChEMBLi CHEMBL2021753.

PTM databases

PhosphoSitei O95235.

Proteomic databases

MaxQBi O95235.
PaxDbi O95235.
PeptideAtlasi O95235.
PRIDEi O95235.

Protocols and materials databases

DNASUi 10112.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000394894 ; ENSP00000378356 ; ENSG00000112984 .
GeneIDi 10112.
KEGGi hsa:10112.
UCSCi uc003lcj.3. human.

Organism-specific databases

CTDi 10112.
GeneCardsi GC05P137542.
HGNCi HGNC:9787. KIF20A.
HPAi HPA036909.
HPA036910.
MIMi 605664. gene.
neXtProti NX_O95235.
PharmGKBi PA34149.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5059.
HOGENOMi HOG000054207.
HOVERGENi HBG052246.
InParanoidi O95235.
KOi K10402.
OMAi RLCEDQN.
OrthoDBi EOG74BJR9.
PhylomeDBi O95235.
TreeFami TF105232.

Enzyme and pathway databases

Reactomei REACT_121399. MHC class II antigen presentation.
REACT_1932. Mitotic Telophase/Cytokinesis.
REACT_25201. Kinesins.

Miscellaneous databases

GeneWikii KIF20A.
GenomeRNAii 10112.
NextBioi 38255.
PROi O95235.
SOURCEi Search...

Gene expression databases

ArrayExpressi O95235.
Bgeei O95235.
CleanExi HS_KIF20A.
Genevestigatori O95235.

Family and domain databases

Gene3Di 3.40.850.10. 2 hits.
InterProi IPR027326. KIF20A.
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR24115. PTHR24115. 1 hit.
PTHR24115:SF352. PTHR24115:SF352. 1 hit.
Pfami PF00225. Kinesin. 1 hit.
[Graphical view ]
PRINTSi PR00380. KINESINHEAVY.
SMARTi SM00129. KISc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Vascular endothelial genes that are responsive to tumor necrosis factor-alpha in vitro are expressed in atherosclerotic lesions, including inhibitor of apoptosis protein-1, stannin, and two novel genes."
    Horrevoets A.J.G., Fontijn R.D., van Zonneveld A.J., de Vries C.J.M., ten Cate J.W., Pannekoek H.
    Blood 93:3418-3431(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Endothelial cell.
  2. "cDNA cloning, expression pattern, genomic structure and chromosomal location of RAB6KIFL, a human kinesin-like gene."
    Lai F., Fernald A.A., Zhao N., Le Beau M.M.
    Gene 248:117-125(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.
  5. "Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis."
    Neef R., Preisinger C., Sutcliffe J., Kopajtich R., Nigg E.A., Mayer T.U., Barr F.A.
    J. Cell Biol. 162:863-875(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PLK1, PHOSPHORYLATION AT SER-528; SER-662 AND SER-668, MUTAGENESIS OF SER-528, FUNCTION.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-528; SER-532; THR-857 AND SER-867, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; SER-14; SER-21; SER-532 AND SER-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKI20A_HUMAN
AccessioniPrimary (citable) accession number: O95235
Secondary accession number(s): D3DQB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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