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O95229 (ZWINT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ZW10 interactor
Alternative name(s):
ZW10-interacting protein 1
Short name=Zwint-1
Gene names
Name:ZWINT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. Ref.4 Ref.5 Ref.7 Ref.8

Subunit structure

Interacts with ZW10 and MIS12. Interacts with the NDC80 subunit of the NDC80 complex specifically during mitosis. Also interacts with CASC5, CETN3, DSN1 and PMF1. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Localizes to kinetochores from late prophase to anaphase. Ref.5 Ref.6 Ref.7 Ref.8

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCentromere
Chromosome
Kinetochore
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Non-traceable author statement PubMed 15504738. Source: UniProtKB

establishment of localization in cell

Inferred from direct assay Ref.5. Source: HGNC

mitotic cell cycle

Traceable author statement. Source: Reactome

mitotic cell cycle checkpoint

Inferred from direct assay Ref.5. Source: HGNC

mitotic sister chromatid segregation

Inferred from direct assay Ref.5. Source: HGNC

phosphatidylinositol-mediated signaling

Non-traceable author statement PubMed 15504738. Source: UniProtKB

spindle organization

Non-traceable author statement PubMed 15504738. Source: UniProtKB

   Cellular_componentcondensed chromosome kinetochore

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from direct assay PubMed 11682612. Source: BHF-UCL

cytosol

Traceable author statement. Source: Reactome

dendrite

Inferred from electronic annotation. Source: Ensembl

kinetochore

Inferred from direct assay PubMed 11682612. Source: BHF-UCL

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein N-terminus binding

Inferred from physical interaction Ref.5. Source: HGNC

protein binding

Inferred from physical interaction Ref.6. Source: UniProtKB

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95229-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95229-2)

The sequence of this isoform differs from the canonical sequence as follows:
     174-220: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 277277ZW10 interactor
PRO_0000066594

Regions

Region80 – 15576Interaction with NDC80 and ZW10
Coiled coil104 – 217114 Potential

Natural variations

Alternative sequence174 – 22047Missing in isoform 2.
VSP_047660
Natural variant41A → S.
Corresponds to variant rs11005328 [ dbSNP | Ensembl ].
VAR_028783
Natural variant1871R → G. Ref.1 Ref.3
Corresponds to variant rs2241666 [ dbSNP | Ensembl ].
VAR_051505

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 31, 2006. Version 2.
Checksum: 3335FBA5731AD0DC

FASTA27731,293
        10         20         30         40         50         60 
MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD KLLCSQLQVA 

        70         80         90        100        110        120 
DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK ATYREHVEAI KIGLTKALTQ 

       130        140        150        160        170        180 
MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG 

       190        200        210        220        230        240 
TQQELDRVFQ KLGNLKQQAE QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP 

       250        260        270 
QQPTRPQEQS TGDTMGRDPG VSFKAVGLQP AGDVNLP 

« Hide

Isoform 2 [UniParc].

Checksum: 673BAD09107D7E3B
Show »

FASTA23025,544

References

« Hide 'large scale' references
[1]"HZwint-1, a novel human kinetochore component that interacts with HZW10."
Starr D.A., Saffery R., Li Z., Simpson A.E., Choo K.H., Yen T.J., Goldberg M.L.
J. Cell Sci. 113:1939-1950(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-187.
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-187.
Tissue: Colon, Lymph and Muscle.
[4]"Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes."
Musio A., Mariani T., Montagna C., Zambroni D., Ascoli C., Ried T., Vezzoni P.
Gene 331:33-40(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling."
Wang H., Hu X., Ding X., Dou Z., Yang Z., Shaw A.W., Teng M., Cleveland D.W., Goldberg M.L., Niu L., Yao X.
J. Biol. Chem. 279:54590-54598(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ZW10, SUBCELLULAR LOCATION.
[6]"A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1."
Obuse C., Iwasaki O., Kiyomitsu T., Goshima G., Toyoda Y., Yanagida M.
Nat. Cell Biol. 6:1135-1141(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MIS12, SUBCELLULAR LOCATION.
[7]"ZW10 links mitotic checkpoint signaling to the structural kinetochore."
Kops G.J.P.L., Kim Y., Weaver B.A.A., Mao Y., McLeod I., Yates J.R. III, Tagaya M., Cleveland D.W.
J. Cell Biol. 169:49-60(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CASC5; CETN3; DSN1; NDC80; MIS12; PMFF1 AND ZW10, SUBCELLULAR LOCATION.
[8]"Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control."
Lin Y.-T., Chen Y., Wu G., Lee W.-H.
Oncogene 25:6901-6914(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NDC80 AND ZW10, SUBCELLULAR LOCATION.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF067656 mRNA. Translation: AAC78629.1.
AC010996 Genomic DNA. No translation available.
BC000411 mRNA. Translation: AAH00411.1.
BC020979 mRNA. Translation: AAH20979.1.
BC110399 mRNA. Translation: AAI10400.1.
CCDSCCDS31205.1. [O95229-2]
CCDS7249.1. [O95229-1]
RefSeqNP_001005413.1. NM_001005413.1. [O95229-2]
NP_008988.2. NM_007057.3. [O95229-1]
NP_127490.1. NM_032997.2. [O95229-1]
XP_005269520.1. XM_005269463.1. [O95229-1]
UniGeneHs.591363.

3D structure databases

ProteinModelPortalO95229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116303. 42 interactions.
IntActO95229. 38 interactions.
MINTMINT-4990180.
STRING9606.ENSP00000363055.

PTM databases

PhosphoSiteO95229.

Proteomic databases

MaxQBO95229.
PaxDbO95229.
PRIDEO95229.

Protocols and materials databases

DNASU11130.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361148; ENSP00000354921; ENSG00000122952. [O95229-2]
ENST00000373944; ENSP00000363055; ENSG00000122952. [O95229-1]
ENST00000395405; ENSP00000378801; ENSG00000122952. [O95229-1]
GeneID11130.
KEGGhsa:11130.
UCSCuc001jjx.1. human. [O95229-1]

Organism-specific databases

CTD11130.
GeneCardsGC10M058116.
HGNCHGNC:13195. ZWINT.
HPACAB033926.
HPA005757.
HPA022264.
MIM609177. gene.
neXtProtNX_O95229.
PharmGKBPA37761.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39831.
HOGENOMHOG000293254.
HOVERGENHBG079391.
InParanoidO95229.
OMAIAAKEQW.
OrthoDBEOG783MW9.
PhylomeDBO95229.
TreeFamTF338101.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.

Gene expression databases

ArrayExpressO95229.
BgeeO95229.
CleanExHS_ZWINT.
GenevestigatorO95229.

Family and domain databases

InterProIPR029092. Zwint-1.
[Graphical view]
PANTHERPTHR31504. PTHR31504. 1 hit.
PfamPF15556. Zwint. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiZWINT.
GenomeRNAi11130.
NextBio42300.
PROO95229.
SOURCESearch...

Entry information

Entry nameZWINT_HUMAN
AccessionPrimary (citable) accession number: O95229
Secondary accession number(s): A6NNV6, Q0D2I3, Q9BWD0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: October 31, 2006
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM