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O95229

- ZWINT_HUMAN

UniProt

O95229 - ZWINT_HUMAN

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Protein

ZW10 interactor

Gene

ZWINT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.4 Publications

GO - Molecular functioni

  1. protein N-terminus binding Source: HGNC

GO - Biological processi

  1. establishment of localization in cell Source: HGNC
  2. mitotic cell cycle Source: Reactome
  3. mitotic cell cycle checkpoint Source: HGNC
  4. mitotic sister chromatid segregation Source: HGNC
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_682. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
ZW10 interactor
Alternative name(s):
ZW10-interacting protein 1
Short name:
Zwint-1
Gene namesi
Name:ZWINT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:13195. ZWINT.

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Localizes to kinetochores from late prophase to anaphase.

GO - Cellular componenti

  1. cytoplasm Source: BHF-UCL
  2. cytosol Source: Reactome
  3. dendrite Source: Ensembl
  4. kinetochore Source: BHF-UCL
  5. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37761.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277ZW10 interactorPRO_0000066594Add
BLAST

Proteomic databases

MaxQBiO95229.
PaxDbiO95229.
PRIDEiO95229.

PTM databases

PhosphoSiteiO95229.

Expressioni

Gene expression databases

BgeeiO95229.
CleanExiHS_ZWINT.
ExpressionAtlasiO95229. baseline and differential.
GenevestigatoriO95229.

Organism-specific databases

HPAiCAB033926.
HPA005757.
HPA022264.

Interactioni

Subunit structurei

Interacts with ZW10 and MIS12. Interacts with the NDC80 subunit of the NDC80 complex specifically during mitosis. Also interacts with CASC5, CETN3, DSN1 and PMF1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BECN1Q144576EBI-1001132,EBI-949378
NDC80O1477711EBI-1001132,EBI-715849
ZW10O432646EBI-1001132,EBI-1001217

Protein-protein interaction databases

BioGridi116303. 54 interactions.
IntActiO95229. 38 interactions.
MINTiMINT-4990180.
STRINGi9606.ENSP00000363055.

Structurei

3D structure databases

ProteinModelPortaliO95229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 15576Interaction with NDC80 and ZW10Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili104 – 217114Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG39831.
GeneTreeiENSGT00390000017639.
HOGENOMiHOG000293254.
HOVERGENiHBG079391.
InParanoidiO95229.
OMAiIAAKEQW.
OrthoDBiEOG783MW9.
PhylomeDBiO95229.
TreeFamiTF338101.

Family and domain databases

InterProiIPR029092. Zwint-1.
[Graphical view]
PANTHERiPTHR31504. PTHR31504. 1 hit.
PfamiPF15556. Zwint. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O95229-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD
60 70 80 90 100
KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK
110 120 130 140 150
ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR
160 170 180 190 200
AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDRVFQ KLGNLKQQAE
210 220 230 240 250
QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS
260 270
TGDTMGRDPG VSFKAVGLQP AGDVNLP
Length:277
Mass (Da):31,293
Last modified:October 31, 2006 - v2
Checksum:i3335FBA5731AD0DC
GO
Isoform 2 (identifier: O95229-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-220: Missing.

Note: No experimental confirmation available.

Show »
Length:230
Mass (Da):25,544
Checksum:i673BAD09107D7E3B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41A → S.
Corresponds to variant rs11005328 [ dbSNP | Ensembl ].
VAR_028783
Natural varianti187 – 1871R → G.2 Publications
Corresponds to variant rs2241666 [ dbSNP | Ensembl ].
VAR_051505

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei174 – 22047Missing in isoform 2. CuratedVSP_047660Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067656 mRNA. Translation: AAC78629.1.
AC010996 Genomic DNA. No translation available.
BC000411 mRNA. Translation: AAH00411.1.
BC020979 mRNA. Translation: AAH20979.1.
BC110399 mRNA. Translation: AAI10400.1.
CCDSiCCDS31205.1. [O95229-2]
CCDS7249.1. [O95229-1]
RefSeqiNP_001005413.1. NM_001005413.1. [O95229-2]
NP_008988.2. NM_007057.3. [O95229-1]
NP_127490.1. NM_032997.2. [O95229-1]
XP_005269520.1. XM_005269463.1. [O95229-1]
UniGeneiHs.591363.

Genome annotation databases

EnsembliENST00000361148; ENSP00000354921; ENSG00000122952. [O95229-2]
ENST00000373944; ENSP00000363055; ENSG00000122952. [O95229-1]
ENST00000395405; ENSP00000378801; ENSG00000122952. [O95229-1]
GeneIDi11130.
KEGGihsa:11130.
UCSCiuc001jjx.1. human. [O95229-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067656 mRNA. Translation: AAC78629.1 .
AC010996 Genomic DNA. No translation available.
BC000411 mRNA. Translation: AAH00411.1 .
BC020979 mRNA. Translation: AAH20979.1 .
BC110399 mRNA. Translation: AAI10400.1 .
CCDSi CCDS31205.1. [O95229-2 ]
CCDS7249.1. [O95229-1 ]
RefSeqi NP_001005413.1. NM_001005413.1. [O95229-2 ]
NP_008988.2. NM_007057.3. [O95229-1 ]
NP_127490.1. NM_032997.2. [O95229-1 ]
XP_005269520.1. XM_005269463.1. [O95229-1 ]
UniGenei Hs.591363.

3D structure databases

ProteinModelPortali O95229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116303. 54 interactions.
IntActi O95229. 38 interactions.
MINTi MINT-4990180.
STRINGi 9606.ENSP00000363055.

PTM databases

PhosphoSitei O95229.

Proteomic databases

MaxQBi O95229.
PaxDbi O95229.
PRIDEi O95229.

Protocols and materials databases

DNASUi 11130.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000361148 ; ENSP00000354921 ; ENSG00000122952 . [O95229-2 ]
ENST00000373944 ; ENSP00000363055 ; ENSG00000122952 . [O95229-1 ]
ENST00000395405 ; ENSP00000378801 ; ENSG00000122952 . [O95229-1 ]
GeneIDi 11130.
KEGGi hsa:11130.
UCSCi uc001jjx.1. human. [O95229-1 ]

Organism-specific databases

CTDi 11130.
GeneCardsi GC10M058116.
HGNCi HGNC:13195. ZWINT.
HPAi CAB033926.
HPA005757.
HPA022264.
MIMi 609177. gene.
neXtProti NX_O95229.
PharmGKBi PA37761.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG39831.
GeneTreei ENSGT00390000017639.
HOGENOMi HOG000293254.
HOVERGENi HBG079391.
InParanoidi O95229.
OMAi IAAKEQW.
OrthoDBi EOG783MW9.
PhylomeDBi O95229.
TreeFami TF338101.

Enzyme and pathway databases

Reactomei REACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_682. Mitotic Prometaphase.

Miscellaneous databases

GeneWikii ZWINT.
GenomeRNAii 11130.
NextBioi 42300.
PROi O95229.
SOURCEi Search...

Gene expression databases

Bgeei O95229.
CleanExi HS_ZWINT.
ExpressionAtlasi O95229. baseline and differential.
Genevestigatori O95229.

Family and domain databases

InterProi IPR029092. Zwint-1.
[Graphical view ]
PANTHERi PTHR31504. PTHR31504. 1 hit.
Pfami PF15556. Zwint. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "HZwint-1, a novel human kinetochore component that interacts with HZW10."
    Starr D.A., Saffery R., Li Z., Simpson A.E., Choo K.H., Yen T.J., Goldberg M.L.
    J. Cell Sci. 113:1939-1950(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-187.
  2. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-187.
    Tissue: Colon, Lymph and Muscle.
  4. "Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes."
    Musio A., Mariani T., Montagna C., Zambroni D., Ascoli C., Ried T., Vezzoni P.
    Gene 331:33-40(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling."
    Wang H., Hu X., Ding X., Dou Z., Yang Z., Shaw A.W., Teng M., Cleveland D.W., Goldberg M.L., Niu L., Yao X.
    J. Biol. Chem. 279:54590-54598(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ZW10, SUBCELLULAR LOCATION.
  6. "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1."
    Obuse C., Iwasaki O., Kiyomitsu T., Goshima G., Toyoda Y., Yanagida M.
    Nat. Cell Biol. 6:1135-1141(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MIS12, SUBCELLULAR LOCATION.
  7. "ZW10 links mitotic checkpoint signaling to the structural kinetochore."
    Kops G.J.P.L., Kim Y., Weaver B.A.A., Mao Y., McLeod I., Yates J.R. III, Tagaya M., Cleveland D.W.
    J. Cell Biol. 169:49-60(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CASC5; CETN3; DSN1; NDC80; MIS12; PMFF1 AND ZW10, SUBCELLULAR LOCATION.
  8. "Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control."
    Lin Y.-T., Chen Y., Wu G., Lee W.-H.
    Oncogene 25:6901-6914(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NDC80 AND ZW10, SUBCELLULAR LOCATION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiZWINT_HUMAN
AccessioniPrimary (citable) accession number: O95229
Secondary accession number(s): A6NNV6, Q0D2I3, Q9BWD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: October 31, 2006
Last modified: October 29, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3