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O95229

- ZWINT_HUMAN

UniProt

O95229 - ZWINT_HUMAN

Protein

ZW10 interactor

Gene

ZWINT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (31 Oct 2006)
      Previous versions | rss
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    Functioni

    Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.4 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein N-terminus binding Source: HGNC

    GO - Biological processi

    1. cell cycle Source: UniProtKB
    2. establishment of localization in cell Source: HGNC
    3. mitotic cell cycle Source: Reactome
    4. mitotic cell cycle checkpoint Source: HGNC
    5. mitotic sister chromatid segregation Source: HGNC
    6. phosphatidylinositol-mediated signaling Source: UniProtKB
    7. spindle organization Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_150471. Separation of Sister Chromatids.
    REACT_682. Mitotic Prometaphase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ZW10 interactor
    Alternative name(s):
    ZW10-interacting protein 1
    Short name:
    Zwint-1
    Gene namesi
    Name:ZWINT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:13195. ZWINT.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Localizes to kinetochores from late prophase to anaphase.

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. cytoplasm Source: BHF-UCL
    3. cytosol Source: Reactome
    4. dendrite Source: Ensembl
    5. kinetochore Source: BHF-UCL
    6. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37761.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 277277ZW10 interactorPRO_0000066594Add
    BLAST

    Proteomic databases

    MaxQBiO95229.
    PaxDbiO95229.
    PRIDEiO95229.

    PTM databases

    PhosphoSiteiO95229.

    Expressioni

    Gene expression databases

    ArrayExpressiO95229.
    BgeeiO95229.
    CleanExiHS_ZWINT.
    GenevestigatoriO95229.

    Organism-specific databases

    HPAiCAB033926.
    HPA005757.
    HPA022264.

    Interactioni

    Subunit structurei

    Interacts with ZW10 and MIS12. Interacts with the NDC80 subunit of the NDC80 complex specifically during mitosis. Also interacts with CASC5, CETN3, DSN1 and PMF1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BECN1Q144576EBI-1001132,EBI-949378
    NDC80O1477711EBI-1001132,EBI-715849
    ZW10O432646EBI-1001132,EBI-1001217

    Protein-protein interaction databases

    BioGridi116303. 42 interactions.
    IntActiO95229. 38 interactions.
    MINTiMINT-4990180.
    STRINGi9606.ENSP00000363055.

    Structurei

    3D structure databases

    ProteinModelPortaliO95229.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 15576Interaction with NDC80 and ZW10Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili104 – 217114Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG39831.
    HOGENOMiHOG000293254.
    HOVERGENiHBG079391.
    InParanoidiO95229.
    OMAiIAAKEQW.
    OrthoDBiEOG783MW9.
    PhylomeDBiO95229.
    TreeFamiTF338101.

    Family and domain databases

    InterProiIPR029092. Zwint-1.
    [Graphical view]
    PANTHERiPTHR31504. PTHR31504. 1 hit.
    PfamiPF15556. Zwint. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O95229-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD    50
    KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK 100
    ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR 150
    AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDRVFQ KLGNLKQQAE 200
    QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS 250
    TGDTMGRDPG VSFKAVGLQP AGDVNLP 277
    Length:277
    Mass (Da):31,293
    Last modified:October 31, 2006 - v2
    Checksum:i3335FBA5731AD0DC
    GO
    Isoform 2 (identifier: O95229-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         174-220: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:230
    Mass (Da):25,544
    Checksum:i673BAD09107D7E3B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti4 – 41A → S.
    Corresponds to variant rs11005328 [ dbSNP | Ensembl ].
    VAR_028783
    Natural varianti187 – 1871R → G.2 Publications
    Corresponds to variant rs2241666 [ dbSNP | Ensembl ].
    VAR_051505

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei174 – 22047Missing in isoform 2. CuratedVSP_047660Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067656 mRNA. Translation: AAC78629.1.
    AC010996 Genomic DNA. No translation available.
    BC000411 mRNA. Translation: AAH00411.1.
    BC020979 mRNA. Translation: AAH20979.1.
    BC110399 mRNA. Translation: AAI10400.1.
    CCDSiCCDS31205.1. [O95229-2]
    CCDS7249.1. [O95229-1]
    RefSeqiNP_001005413.1. NM_001005413.1. [O95229-2]
    NP_008988.2. NM_007057.3. [O95229-1]
    NP_127490.1. NM_032997.2. [O95229-1]
    XP_005269520.1. XM_005269463.1. [O95229-1]
    UniGeneiHs.591363.

    Genome annotation databases

    EnsembliENST00000361148; ENSP00000354921; ENSG00000122952. [O95229-2]
    ENST00000373944; ENSP00000363055; ENSG00000122952. [O95229-1]
    ENST00000395405; ENSP00000378801; ENSG00000122952. [O95229-1]
    GeneIDi11130.
    KEGGihsa:11130.
    UCSCiuc001jjx.1. human. [O95229-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067656 mRNA. Translation: AAC78629.1 .
    AC010996 Genomic DNA. No translation available.
    BC000411 mRNA. Translation: AAH00411.1 .
    BC020979 mRNA. Translation: AAH20979.1 .
    BC110399 mRNA. Translation: AAI10400.1 .
    CCDSi CCDS31205.1. [O95229-2 ]
    CCDS7249.1. [O95229-1 ]
    RefSeqi NP_001005413.1. NM_001005413.1. [O95229-2 ]
    NP_008988.2. NM_007057.3. [O95229-1 ]
    NP_127490.1. NM_032997.2. [O95229-1 ]
    XP_005269520.1. XM_005269463.1. [O95229-1 ]
    UniGenei Hs.591363.

    3D structure databases

    ProteinModelPortali O95229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116303. 42 interactions.
    IntActi O95229. 38 interactions.
    MINTi MINT-4990180.
    STRINGi 9606.ENSP00000363055.

    PTM databases

    PhosphoSitei O95229.

    Proteomic databases

    MaxQBi O95229.
    PaxDbi O95229.
    PRIDEi O95229.

    Protocols and materials databases

    DNASUi 11130.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361148 ; ENSP00000354921 ; ENSG00000122952 . [O95229-2 ]
    ENST00000373944 ; ENSP00000363055 ; ENSG00000122952 . [O95229-1 ]
    ENST00000395405 ; ENSP00000378801 ; ENSG00000122952 . [O95229-1 ]
    GeneIDi 11130.
    KEGGi hsa:11130.
    UCSCi uc001jjx.1. human. [O95229-1 ]

    Organism-specific databases

    CTDi 11130.
    GeneCardsi GC10M058116.
    HGNCi HGNC:13195. ZWINT.
    HPAi CAB033926.
    HPA005757.
    HPA022264.
    MIMi 609177. gene.
    neXtProti NX_O95229.
    PharmGKBi PA37761.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG39831.
    HOGENOMi HOG000293254.
    HOVERGENi HBG079391.
    InParanoidi O95229.
    OMAi IAAKEQW.
    OrthoDBi EOG783MW9.
    PhylomeDBi O95229.
    TreeFami TF338101.

    Enzyme and pathway databases

    Reactomei REACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_150471. Separation of Sister Chromatids.
    REACT_682. Mitotic Prometaphase.

    Miscellaneous databases

    GeneWikii ZWINT.
    GenomeRNAii 11130.
    NextBioi 42300.
    PROi O95229.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95229.
    Bgeei O95229.
    CleanExi HS_ZWINT.
    Genevestigatori O95229.

    Family and domain databases

    InterProi IPR029092. Zwint-1.
    [Graphical view ]
    PANTHERi PTHR31504. PTHR31504. 1 hit.
    Pfami PF15556. Zwint. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "HZwint-1, a novel human kinetochore component that interacts with HZW10."
      Starr D.A., Saffery R., Li Z., Simpson A.E., Choo K.H., Yen T.J., Goldberg M.L.
      J. Cell Sci. 113:1939-1950(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-187.
    2. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-187.
      Tissue: Colon, Lymph and Muscle.
    4. "Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes."
      Musio A., Mariani T., Montagna C., Zambroni D., Ascoli C., Ried T., Vezzoni P.
      Gene 331:33-40(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling."
      Wang H., Hu X., Ding X., Dou Z., Yang Z., Shaw A.W., Teng M., Cleveland D.W., Goldberg M.L., Niu L., Yao X.
      J. Biol. Chem. 279:54590-54598(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ZW10, SUBCELLULAR LOCATION.
    6. "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1."
      Obuse C., Iwasaki O., Kiyomitsu T., Goshima G., Toyoda Y., Yanagida M.
      Nat. Cell Biol. 6:1135-1141(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MIS12, SUBCELLULAR LOCATION.
    7. "ZW10 links mitotic checkpoint signaling to the structural kinetochore."
      Kops G.J.P.L., Kim Y., Weaver B.A.A., Mao Y., McLeod I., Yates J.R. III, Tagaya M., Cleveland D.W.
      J. Cell Biol. 169:49-60(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CASC5; CETN3; DSN1; NDC80; MIS12; PMFF1 AND ZW10, SUBCELLULAR LOCATION.
    8. "Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control."
      Lin Y.-T., Chen Y., Wu G., Lee W.-H.
      Oncogene 25:6901-6914(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NDC80 AND ZW10, SUBCELLULAR LOCATION.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiZWINT_HUMAN
    AccessioniPrimary (citable) accession number: O95229
    Secondary accession number(s): A6NNV6, Q0D2I3, Q9BWD0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2002
    Last sequence update: October 31, 2006
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3