O95219 (SNX4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorting nexin-4

Gene names

Name:

SNX4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in several stages of intracellular trafficking. Plays a role in recycling endocytosed transferrin receptor and prevent its degradation. Ref.5
Subunit structure	Interacts with WWC1/KIBRA. Identified in a complex with WWC1/KIBRA and dynein components DYNLL1 and DYNC1I2. Ref.5
Subcellular location	Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Also detected on a juxtanuclear endocytic recycling compartment (ERC). Ref.5
Domain	The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization.
Sequence similarities	Belongs to the sorting nexin family. Contains 1 PX (phox homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Membrane
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell communication Inferred from electronic annotation. Source: InterPro endocytic recycling Inferred from mutant phenotype Ref.5. Source: UniProtKB endocytosis Traceable author statement Ref.1. Source: ProtInc protein transport Inferred from mutant phenotype Ref.5. Source: UniProtKB
Cellular_component	cytoplasmic dynein complex Inferred from direct assay Ref.5. Source: UniProtKB early endosome membrane Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	phosphatidylinositol binding Inferred from mutant phenotype Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Sorting nexin-4		PRO_0000213842
Regions
	Domain	61 – 187	127	PX
Sites
	Binding site	106	1	Phosphatidylinositol 3-phosphate By similarity
	Binding site	108	1	Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	132	1	Phosphatidylinositol 3-phosphate By similarity
	Binding site	154	1	Phosphatidylinositol 3-phosphate By similarity
Amino acid modifications
	Modified residue	22	1	Phosphoserine Ref.7
Experimental info
	Mutagenesis	132	1	K → A: Abolishes phosphatidylinositol phosphate binding. Abolishes endosomal location. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O95219 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3D5B52AC52A07686	FASTA	450	51,909
	10 20 30 40 50 60 MEQAPPDPER QLQPAPLEPL GSPDAGLGAA VGKEAEGAGE ESSGVDTMTH NNFWLKKIEI 70 80 90 100 110 120 SVSEAEKRTG RNAMNMQETY TAYLIETRSV EHTDGQSVLT DSLWRRYSEF ELLRSYLLVY 130 140 150 160 170 180 YPHIVVPPLP EKRAEFVWHK LSADNMDPDF VERRRIGLEN FLLRIASHPI LCRDKIFYLF 190 200 210 220 230 240 LTQEGNWKET VNETGFQLKA DSRLKALNAT FRVKNPDKRF TDLKHYSDEL QSVISHLLRV 250 260 270 280 290 300 RARVADRLYG VYKVHGNYGR VFSEWSAIEK EMGDGLQSAG HHMDVYASSI DDILEDEEHY 310 320 330 340 350 360 ADQLKEYLFY AEALRAVCRK HELMQYDLEM AAQDLASKKQ QCEELVTGTV RTFSLKGMTT 370 380 390 400 410 420 KLFGQETPEQ REARIKVLEE QINEGEQQLK SKNLEGREFV KNAWADIERF KEQKNRDLKE 430 440 450 ALISYAVMQI SMCKKGIQVW TNAKECFSKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a family of sorting nexin molecules and characterization of their association with receptors." Haft C.R., de la Luz Sierra M., Barr V.A., Haft D.H., Taylor S.I. Mol. Cell. Biol. 18:7278-7287(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[5]	"SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment." Traer C.J., Rutherford A.C., Palmer K.J., Wassmer T., Oakley J., Attar N., Carlton J.G., Kremerskothen J., Stephens D.J., Cullen P.J. Nat. Cell Biol. 9:1370-1380(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH WWC1/KIBRA, MUTAGENESIS OF LYS-132, SUBCELLULAR LOCATION.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF065485 mRNA. Translation: AAC83149.1. AK001835 mRNA. Translation: BAG50982.1. CH471052 Genomic DNA. Translation: EAW79390.1. CH471052 Genomic DNA. Translation: EAW79391.1. CH471052 Genomic DNA. Translation: EAW79393.1. BC018762 mRNA. Translation: AAH18762.1.
IPI	IPI00029403.
RefSeq	NP_003785.1. NM_003794.3.
UniGene	Hs.507243.
3D structure databases
ProteinModelPortal	O95219.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95219. 7 interactions.
MINT	MINT-263255.
STRING	9606.ENSP00000251775.
PTM databases
PhosphoSite	O95219.
2D gel databases
DOSAC-COBS-2DPAGE	O95219.
Proteomic databases
PaxDb	O95219.
PRIDE	O95219.
Protocols and materials databases
DNASU	8723.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000251775; ENSP00000251775; ENSG00000114520.
GeneID	8723.
KEGG	hsa:8723.
UCSC	uc003eib.3. human.
Organism-specific databases
CTD	8723.
GeneCards	GC03M125196.
HGNC	HGNC:11175. SNX4.
HPA	HPA005709.
MIM	605931. gene.
neXtProt	NX_O95219.
PharmGKB	PA36014.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5391.
HOGENOM	HOG000007933.
HOVERGEN	HBG017826.
InParanoid	O95219.
OMA	MDVYAAS.
OrthoDB	EOG4QC15G.
PhylomeDB	O95219.
Gene expression databases
ArrayExpress	O95219.
Bgee	O95219.
CleanEx	HS_SNX4.
Genevestigator	O95219.
GermOnline	ENSG00000114520. Homo sapiens.
Family and domain databases
Gene3D	3.30.1520.10. 1 hit.
InterPro	IPR001683. Phox. [Graphical view]
Pfam	PF00787. PX. 1 hit. [Graphical view]
SMART	SM00312. PX. 1 hit. [Graphical view]
SUPFAM	SSF64268. PX. 1 hit.
PROSITE	PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SNX4. human.
GenomeRNAi	8723.
NextBio	32719.
SOURCE	Search...

Entry information

Entry name

SNX4_HUMAN

Accession

Primary (citable) accession number: O95219
Secondary accession number(s): B3KMH0, D3DNA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.