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Protein

Epsin-2

Gene

EPN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the formation of clathrin-coated invaginations and endocytosis.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei11Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei25Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei30Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei63Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei73Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • membrane organization Source: Reactome
  • negative regulation of sprouting angiogenesis Source: BHF-UCL
  • negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
  • positive regulation of Notch signaling pathway Source: BHF-UCL

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-2
Alternative name(s):
EPS-15-interacting protein 2
Gene namesi
Name:EPN2
Synonyms:KIAA1065
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000072134.15
HGNCiHGNC:18639 EPN2
MIMi607263 gene
neXtProtiNX_O95208

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Organism-specific databases

DisGeNETi22905
OpenTargetsiENSG00000072134
PharmGKBiPA38615

Polymorphism and mutation databases

BioMutaiEPN2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745161 – 641Epsin-2Add BLAST641

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170Omega-N-methylarginineBy similarity1
Modified residuei173PhosphoserineCombined sources1
Modified residuei192PhosphoserineBy similarity1
Modified residuei195PhosphoserineBy similarity1
Modified residuei486PhosphoserineBy similarity1
Modified residuei508PhosphothreonineBy similarity1
Modified residuei570PhosphoserineCombined sources1
Isoform 3 (identifier: O95208-3)
Modified residuei153PhosphoserineCombined sources1
Modified residuei156PhosphoserineCombined sources1
Isoform 2 (identifier: O95208-2)
Modified residuei192PhosphoserineCombined sources1
Modified residuei195PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO95208
MaxQBiO95208
PaxDbiO95208
PeptideAtlasiO95208
PRIDEiO95208

PTM databases

iPTMnetiO95208
PhosphoSitePlusiO95208

Miscellaneous databases

PMAP-CutDBiO95208

Expressioni

Tissue specificityi

Highest expression is found in brain. Detected at lower levels in lung and liver.1 Publication

Gene expression databases

BgeeiENSG00000072134
CleanExiHS_EPN2
ExpressionAtlasiO95208 baseline and differential
GenevisibleiO95208 HS

Organism-specific databases

HPAiHPA049809
HPA053360

Interactioni

Subunit structurei

Binds EPS15 (By similarity). Interacts with ITSN1 (By similarity). Binds AP-2 and clathrin. Interacts with UBQLN2.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116569, 15 interactors
ELMiO95208
IntActiO95208, 35 interactors
STRINGi9606.ENSP00000320543

Structurei

3D structure databases

ProteinModelPortaliO95208
SMRiO95208
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 144ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini275 – 294UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini300 – 319UIM 2PROSITE-ProRule annotationAdd BLAST20
Repeati352 – 35413
Repeati364 – 36623
Repeati377 – 37933
Repeati391 – 39343
Repeati409 – 41153
Repeati427 – 42963
Repeati537 – 53913
Repeati552 – 55423
Repeati637 – 63933

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni352 – 6396 X 3 AA repeats of [DE]-P-WAdd BLAST288
Regioni537 – 6393 X 3 AA repeats of N-P-FAdd BLAST103

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.

Sequence similaritiesi

Belongs to the epsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2056 Eukaryota
ENOG410XSM0 LUCA
GeneTreeiENSGT00550000074611
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiO95208
KOiK12471
OMAiEQEYGKS
OrthoDBiEOG091G0L45
PhylomeDBiO95208
TreeFamiTF313361

Family and domain databases

Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR027319 Epsin-2_metazoa
IPR003903 UIM_dom
PANTHERiPTHR12276:SF50 PTHR12276:SF50, 1 hit
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 2 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 2 hits

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95208-1) [UniParc]FASTAAdd to basket
Also known as: 2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV
60 70 80 90 100
VAFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI
110 120 130 140 150
FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KAERAQALKT
160 170 180 190 200
KERMAQVATG MGSNQITFGR GSSQPNLSTS HSEQEYGKAG GSPASYHGSP
210 220 230 240 250
EASLCPQHRT GAPLGQSEEL QPLSQRHPFL PHLGLASRPN GDWSQPCLTC
260 270 280 290 300
DRAARATSPR VSSELEQARP QTSGEEELQL QLALAMSREV AEQEERLRRG
310 320 330 340 350
DDLRLQMALE ESRRDTVKIP KKKEHGSLPQ QTTLLDLMDA LPSSGPAAQK
360 370 380 390 400
AEPWGPSAST NQTNPWGGPA APASTSDPWP SFGTKPAASI DPWGVPTGAT
410 420 430 440 450
VQSVPKNSDP WAASQQPASS AGKRASDAWG AVSTTKPVSV SGSFELFSNL
460 470 480 490 500
NGTIKDDFSE FDNLRTSKKT AESVTSLPSQ NNGTTSPDPF ESQPLTVASS
510 520 530 540 550
KPSSARKTPE SFLGPNAALV NLDSLVTRPA PPAQSLNPFL APGAPATSAP
560 570 580 590 600
VNPFQVNQPQ PLTLNQLRGS PVLGTSTSFG PGPGVESMAV ASMTSAAPQP
610 620 630 640
ALGATGSSLT PLGPAMMNMV GSVGIPPSAA QATGTTNPFL L
Length:641
Mass (Da):68,482
Last modified:December 16, 2008 - v3
Checksum:i0BE24CBD824F357A
GO
Isoform 2 (identifier: O95208-2) [UniParc]FASTAAdd to basket
Also known as: 2a

The sequence of this isoform differs from the canonical sequence as follows:
     200-256: Missing.

Show »
Length:584
Mass (Da):62,300
Checksum:iD668242ECACD26EF
GO
Isoform 3 (identifier: O95208-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.
     200-256: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:545
Mass (Da):57,919
Checksum:iC8D6661429926997
GO
Isoform 4 (identifier: O95208-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-285: Missing.

Note: No experimental confirmation available.
Show »
Length:356
Mass (Da):36,729
Checksum:iEF6741C03E5744A3
GO

Sequence cautioni

The sequence BAA83017 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAG52540 differs from that shown. Reason: Erroneous termination at position 324. Translated as Glu.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256A → AS in AAC78609 (PubMed:10567358).Curated1
Sequence conflicti547T → N in AAC78608 (PubMed:10567358).Curated1
Sequence conflicti547T → N in AAC78609 (PubMed:10567358).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053080401V → A5 PublicationsCorresponds to variant dbSNP:rs6587220Ensembl.1
Natural variantiVAR_047923531P → T. Corresponds to variant dbSNP:rs1062727Ensembl.1
Natural variantiVAR_053081532P → T. Corresponds to variant dbSNP:rs1062727Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0470031 – 285Missing in isoform 4. 1 PublicationAdd BLAST285
Alternative sequenceiVSP_0091541 – 39Missing in isoform 3. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_009155200 – 256Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062084 mRNA Translation: AAC78608.1
AF062085 mRNA Translation: AAC78609.1
AB028988 mRNA Translation: BAA83017.2 Different initiation.
AK001996 mRNA Translation: BAG51000.1
AK024115 mRNA Translation: BAB14831.1
AK092366 mRNA Translation: BAG52540.1 Sequence problems.
AC106017 Genomic DNA No translation available.
AC124066 Genomic DNA No translation available.
CH471212 Genomic DNA Translation: EAW50876.1
BC093972 mRNA Translation: AAH93972.1
BC093974 mRNA Translation: AAH93974.1
CCDSiCCDS11203.1 [O95208-1]
CCDS11204.1 [O95208-2]
CCDS42277.1 [O95208-5]
RefSeqiNP_001096134.1, NM_001102664.1 [O95208-5]
NP_055779.2, NM_014964.4 [O95208-1]
NP_683723.2, NM_148921.3 [O95208-2]
UniGeneiHs.743983

Genome annotation databases

EnsembliENST00000314728; ENSP00000320543; ENSG00000072134 [O95208-1]
ENST00000347697; ENSP00000261495; ENSG00000072134 [O95208-2]
ENST00000395618; ENSP00000378980; ENSG00000072134 [O95208-5]
ENST00000395620; ENSP00000378982; ENSG00000072134 [O95208-2]
GeneIDi22905
KEGGihsa:22905
UCSCiuc002gvd.5 human [O95208-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiEPN2_HUMAN
AccessioniPrimary (citable) accession number: O95208
Secondary accession number(s): A8MTV8
, B3KRX8, E9PBC2, O95207, Q52LD0, Q9H7Z2, Q9UPT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: December 16, 2008
Last modified: May 23, 2018
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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