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O95196

- CSPG5_HUMAN

UniProt

O95196 - CSPG5_HUMAN

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Protein

Chondroitin sulfate proteoglycan 5

Gene
CSPG5, CALEB, NGC
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.1 Publication

GO - Molecular functioni

  1. growth factor activity Source: UniProtKB
  2. protein binding Source: UniProtKB

GO - Biological processi

  1. axon regeneration Source: Ensembl
  2. carbohydrate metabolic process Source: Reactome
  3. chondroitin sulfate biosynthetic process Source: Reactome
  4. chondroitin sulfate catabolic process Source: Reactome
  5. chondroitin sulfate metabolic process Source: Reactome
  6. dermatan sulfate biosynthetic process Source: Reactome
  7. glycosaminoglycan metabolic process Source: Reactome
  8. intracellular transport Source: UniProtKB
  9. nervous system development Source: UniProtKB
  10. regulation of growth Source: UniProtKB-KW
  11. regulation of synaptic transmission Source: Ensembl
  12. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Growth regulation, Neurogenesis

Enzyme and pathway databases

ReactomeiREACT_120800. Dermatan sulfate biosynthesis.
REACT_120888. CS/DS degradation.
REACT_120989. Chondroitin sulfate biosynthesis.
REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate proteoglycan 5
Alternative name(s):
Acidic leucine-rich EGF-like domain-containing brain protein
Neuroglycan C
Gene namesi
Name:CSPG5
Synonyms:CALEB, NGC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:2467. CSPG5.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Golgi apparatus membrane; Single-pass type I membrane protein By similarity
Note: In neurons, localizes to synaptic junctions By similarity. Also detected in the endoplasmic reticulum and the Golgi By similarity. Partially enriched in lipid rafts By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 423393Extracellular Reviewed predictionAdd
BLAST
Transmembranei424 – 44421Helical; Reviewed predictionAdd
BLAST
Topological domaini445 – 566122Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. extracellular region Source: Reactome
  3. Golgi apparatus Source: UniProtKB
  4. Golgi-associated vesicle membrane Source: UniProtKB
  5. Golgi lumen Source: Reactome
  6. integral component of membrane Source: UniProtKB
  7. integral component of plasma membrane Source: ProtInc
  8. lysosomal lumen Source: Reactome
  9. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26965.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030 Reviewed predictionAdd
BLAST
Chaini31 – 566536Chondroitin sulfate proteoglycan 5PRO_0000042151Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...) Reviewed prediction
Glycosylationi117 – 1171O-linked (Xyl...) (chondroitin sulfate) By similarity
Glycosylationi165 – 1651O-linked (GalNAc...)1 Publication
Disulfide bondi374 ↔ 387 By similarity
Disulfide bondi381 ↔ 397 By similarity
Disulfide bondi399 ↔ 412 By similarity

Post-translational modificationi

N-glycosylated By similarity.1 Publication
O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation By similarity.1 Publication
Phosphorylated; in intracellular and extracellular parts By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

PaxDbiO95196.
PRIDEiO95196.

PTM databases

PhosphoSiteiO95196.

Expressioni

Tissue specificityi

Restricted to brain (at protein level).1 Publication

Developmental stagei

Expressed in brain of 3 months, 5 and 10-year-old individuals.1 Publication

Gene expression databases

ArrayExpressiO95196.
BgeeiO95196.
CleanExiHS_CSPG5.
GenevestigatoriO95196.

Interactioni

Subunit structurei

Binds TNR and probably TNC By similarity. Interacts with ERBB3 and GOPC.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GopcQ8BH603EBI-296349,EBI-296357From a different organism.

Protein-protein interaction databases

BioGridi115917. 1 interaction.
IntActiO95196. 1 interaction.
STRINGi9606.ENSP00000264723.

Structurei

3D structure databases

ProteinModelPortaliO95196.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini371 – 41343EGF-likeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni264 – 30138Interaction with TNC and TNR By similarityAdd
BLAST
Regioni442 – 46019Interaction with GOPCAdd
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG39505.
HOGENOMiHOG000112020.
HOVERGENiHBG081361.
InParanoidiO95196.
KOiK08116.
OMAiLEVNCLQ.
OrthoDBiEOG7VX8WW.
PhylomeDBiO95196.
TreeFamiTF338636.

Family and domain databases

InterProiIPR010555. Chon_Sulph_att.
IPR000742. EG-like_dom.
IPR009505. Neural_ProG_Cyt.
[Graphical view]
PfamiPF06566. Chon_Sulph_att. 1 hit.
PF06567. Neural_ProG_Cyt. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O95196-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGRAGGGGPG RGPPPLLLFL GAALVLASGA VPAREAGSAV EAEELVKGSP    50
AWEPPANDTR EEAGPPAAGE DEASWTAPGG ELAGPEEVLQ ESAAVTGTAW 100
LEADSPGLGG VTAEAGSGDA QALPATLQAP HEVLGQSIMP PAIPEATEAS 150
GPPSPTPGDK LSPASELPKE SPLEVWLNLG GSTPDPQGPE LTYPFQGTLE 200
PQPASDIIDI DYFEGLDGEG RGADLGSFPG SPGTSENHPD TEGETPSWSL 250
LDLYDDFTPF DESDFYPTTS FYDDLDEEEE EEEDDKDAVG GGDLEDENEL 300
LVPTGKPGLG PGTGQPTSRW HAVPPQHTLG SVPGSSIALR PRPGEPGRDL 350
ASSENGTECR SGFVRHNGSC RSVCDLFPSY CHNGGQCYLV ENIGAFCRCN 400
TQDYIWHKGM RCESIITDFQ VMCVAVGSAA LVLLLLFMMT VFFAKKLYLL 450
KTENTKLRRT NKFRTPSELH NDNFSLSTIA EGSHPNVRKL CNTPRTSSPH 500
ARALAHYDNV ICQDDPSAPH KIQEVLKSCL KEEESFNIQN SMSPKLEGGK 550
GDQADLDVNC LQNNLT 566
Length:566
Mass (Da):60,016
Last modified:May 5, 2009 - v3
Checksum:iDD8DA045C1BE31E5
GO
Isoform 2 (identifier: O95196-2) [UniParc]FASTAAdd to Basket

Also known as: CSPG5-I

The sequence of this isoform differs from the canonical sequence as follows:
     487-513: Missing.

Show »
Length:539
Mass (Da):56,983
Checksum:iBBD8B64B93091DDF
GO
Isoform 3 (identifier: O95196-3) [UniParc]FASTAAdd to Basket

Also known as: CSPG5-II

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.
     487-513: Missing.

Show »
Length:401
Mass (Da):43,621
Checksum:i9F0D31F21854FCEB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti188 – 1881G → V.2 Publications
Corresponds to variant rs3732530 [ dbSNP | Ensembl ].
VAR_055089
Natural varianti417 – 4171T → P.
Corresponds to variant rs34016925 [ dbSNP | Ensembl ].
VAR_055090

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 138138Missing in isoform 3. VSP_015760Add
BLAST
Alternative sequencei487 – 51327Missing in isoform 2 and isoform 3. VSP_015761Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF059274 mRNA. Translation: AAC69612.1.
AF461087 mRNA. Translation: AAQ04774.1.
AF461088 mRNA. Translation: AAQ04775.1.
AF461089 mRNA. Translation: AAQ04776.1.
AC099778 Genomic DNA. No translation available.
AC112512 Genomic DNA. No translation available.
CCDSiCCDS2757.1. [O95196-2]
CCDS56252.1. [O95196-3]
CCDS56253.1. [O95196-1]
RefSeqiNP_001193872.1. NM_001206943.1. [O95196-1]
NP_001193873.1. NM_001206944.1.
NP_006565.2. NM_006574.3. [O95196-2]
UniGeneiHs.45127.

Genome annotation databases

EnsembliENST00000264723; ENSP00000264723; ENSG00000114646. [O95196-2]
ENST00000383738; ENSP00000373244; ENSG00000114646. [O95196-1]
ENST00000456150; ENSP00000392096; ENSG00000114646. [O95196-3]
GeneIDi10675.
KEGGihsa:10675.
UCSCiuc003crn.3. human. [O95196-3]
uc003cro.4. human. [O95196-2]
uc003crp.4. human. [O95196-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF059274 mRNA. Translation: AAC69612.1 .
AF461087 mRNA. Translation: AAQ04774.1 .
AF461088 mRNA. Translation: AAQ04775.1 .
AF461089 mRNA. Translation: AAQ04776.1 .
AC099778 Genomic DNA. No translation available.
AC112512 Genomic DNA. No translation available.
CCDSi CCDS2757.1. [O95196-2 ]
CCDS56252.1. [O95196-3 ]
CCDS56253.1. [O95196-1 ]
RefSeqi NP_001193872.1. NM_001206943.1. [O95196-1 ]
NP_001193873.1. NM_001206944.1.
NP_006565.2. NM_006574.3. [O95196-2 ]
UniGenei Hs.45127.

3D structure databases

ProteinModelPortali O95196.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115917. 1 interaction.
IntActi O95196. 1 interaction.
STRINGi 9606.ENSP00000264723.

PTM databases

PhosphoSitei O95196.

Proteomic databases

PaxDbi O95196.
PRIDEi O95196.

Protocols and materials databases

DNASUi 10675.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000264723 ; ENSP00000264723 ; ENSG00000114646 . [O95196-2 ]
ENST00000383738 ; ENSP00000373244 ; ENSG00000114646 . [O95196-1 ]
ENST00000456150 ; ENSP00000392096 ; ENSG00000114646 . [O95196-3 ]
GeneIDi 10675.
KEGGi hsa:10675.
UCSCi uc003crn.3. human. [O95196-3 ]
uc003cro.4. human. [O95196-2 ]
uc003crp.4. human. [O95196-1 ]

Organism-specific databases

CTDi 10675.
GeneCardsi GC03M047603.
H-InvDB HIX0003276.
HGNCi HGNC:2467. CSPG5.
MIMi 606775. gene.
neXtProti NX_O95196.
PharmGKBi PA26965.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG39505.
HOGENOMi HOG000112020.
HOVERGENi HBG081361.
InParanoidi O95196.
KOi K08116.
OMAi LEVNCLQ.
OrthoDBi EOG7VX8WW.
PhylomeDBi O95196.
TreeFami TF338636.

Enzyme and pathway databases

Reactomei REACT_120800. Dermatan sulfate biosynthesis.
REACT_120888. CS/DS degradation.
REACT_120989. Chondroitin sulfate biosynthesis.
REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

GenomeRNAii 10675.
NextBioi 40593.
PROi O95196.
SOURCEi Search...

Gene expression databases

ArrayExpressi O95196.
Bgeei O95196.
CleanExi HS_CSPG5.
Genevestigatori O95196.

Family and domain databases

InterProi IPR010555. Chon_Sulph_att.
IPR000742. EG-like_dom.
IPR009505. Neural_ProG_Cyt.
[Graphical view ]
Pfami PF06566. Chon_Sulph_att. 1 hit.
PF06567. Neural_ProG_Cyt. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and chromosomal mapping of the human gene of neuroglycan C (NGC), a neural transmembrane chondroitin sulfate proteoglycan with an EGF module."
    Yasuda Y., Tokita Y., Aono S., Matsui F., Ono T., Sonta S., Watanabe E., Nakanishi Y., Oohira A.
    Neurosci. Res. 32:313-322(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANT VAL-188.
    Tissue: Brain.
  2. "Expression of neuroglycan C (NGC), a transmembrane chondroitin sulfate proteoglycan with an EGF module, in the human brain."
    Aono S., Tokita Y., Yamauchi S., Shuo T., Matsui F., Yasuda Y., Keino H., Shimada A., Kishikawa M., Asai M., Oohira A.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), VARIANT VAL-188.
  3. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "CALEB/NGC interacts with the Golgi-associated protein PIST."
    Hassel B., Schreff M., Stuebe E.-M., Blaich U., Schumacher S.
    J. Biol. Chem. 278:40136-40143(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOPC.
  5. Cited for: INTERACTION WITH ERBB3, FUNCTION.
  6. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
    Halim A., Ruetschi U., Larson G., Nilsson J.
    J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-165, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiCSPG5_HUMAN
AccessioniPrimary (citable) accession number: O95196
Secondary accession number(s): Q71M39, Q71M40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: May 5, 2009
Last modified: September 3, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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