Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O95196

- CSPG5_HUMAN

UniProt

O95196 - CSPG5_HUMAN

Protein

Chondroitin sulfate proteoglycan 5

Gene

CSPG5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (05 May 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.1 Publication

    GO - Molecular functioni

    1. growth factor activity Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. axon regeneration Source: Ensembl
    2. carbohydrate metabolic process Source: Reactome
    3. chondroitin sulfate biosynthetic process Source: Reactome
    4. chondroitin sulfate catabolic process Source: Reactome
    5. chondroitin sulfate metabolic process Source: Reactome
    6. dermatan sulfate biosynthetic process Source: Reactome
    7. glycosaminoglycan metabolic process Source: Reactome
    8. intracellular transport Source: UniProtKB
    9. nervous system development Source: UniProtKB
    10. regulation of growth Source: UniProtKB-KW
    11. regulation of synaptic transmission Source: Ensembl
    12. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Growth regulation, Neurogenesis

    Enzyme and pathway databases

    ReactomeiREACT_120800. Dermatan sulfate biosynthesis.
    REACT_120888. CS/DS degradation.
    REACT_120989. Chondroitin sulfate biosynthesis.
    REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chondroitin sulfate proteoglycan 5
    Alternative name(s):
    Acidic leucine-rich EGF-like domain-containing brain protein
    Neuroglycan C
    Gene namesi
    Name:CSPG5
    Synonyms:CALEB, NGC
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:2467. CSPG5.

    Subcellular locationi

    Cell membrane By similarity; Single-pass type I membrane protein By similarity. Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity. Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
    Note: In neurons, localizes to synaptic junctions. Also detected in the endoplasmic reticulum and the Golgi. Partially enriched in lipid rafts.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. extracellular region Source: Reactome
    3. Golgi apparatus Source: UniProtKB
    4. Golgi-associated vesicle membrane Source: UniProtKB
    5. Golgi lumen Source: Reactome
    6. integral component of membrane Source: UniProtKB
    7. integral component of plasma membrane Source: ProtInc
    8. lysosomal lumen Source: Reactome
    9. membrane Source: ProtInc

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26965.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 566536Chondroitin sulfate proteoglycan 5PRO_0000042151Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi117 – 1171O-linked (Xyl...) (chondroitin sulfate)By similarity
    Glycosylationi165 – 1651O-linked (GalNAc...)1 Publication
    Disulfide bondi374 ↔ 387By similarity
    Disulfide bondi381 ↔ 397By similarity
    Disulfide bondi399 ↔ 412By similarity

    Post-translational modificationi

    N-glycosylated.By similarity
    O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation By similarity.By similarity
    Phosphorylated; in intracellular and extracellular parts.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

    Proteomic databases

    PaxDbiO95196.
    PRIDEiO95196.

    PTM databases

    PhosphoSiteiO95196.

    Expressioni

    Tissue specificityi

    Restricted to brain (at protein level).1 Publication

    Developmental stagei

    Expressed in brain of 3 months, 5 and 10-year-old individuals.1 Publication

    Gene expression databases

    ArrayExpressiO95196.
    BgeeiO95196.
    CleanExiHS_CSPG5.
    GenevestigatoriO95196.

    Interactioni

    Subunit structurei

    Binds TNR and probably TNC By similarity. Interacts with ERBB3 and GOPC.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GopcQ8BH603EBI-296349,EBI-296357From a different organism.

    Protein-protein interaction databases

    BioGridi115917. 1 interaction.
    IntActiO95196. 1 interaction.
    STRINGi9606.ENSP00000264723.

    Structurei

    3D structure databases

    ProteinModelPortaliO95196.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini31 – 423393ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini445 – 566122CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei424 – 44421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini371 – 41343EGF-likeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni264 – 30138Interaction with TNC and TNRBy similarityAdd
    BLAST
    Regioni442 – 46019Interaction with GOPCAdd
    BLAST

    Sequence similaritiesi

    Contains 1 EGF-like domain.Curated

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG39505.
    HOGENOMiHOG000112020.
    HOVERGENiHBG081361.
    InParanoidiO95196.
    KOiK08116.
    OMAiLEVNCLQ.
    OrthoDBiEOG7VX8WW.
    PhylomeDBiO95196.
    TreeFamiTF338636.

    Family and domain databases

    InterProiIPR010555. Chon_Sulph_att.
    IPR000742. EG-like_dom.
    IPR009505. Neural_ProG_Cyt.
    [Graphical view]
    PfamiPF06566. Chon_Sulph_att. 1 hit.
    PF06567. Neural_ProG_Cyt. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O95196-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGRAGGGGPG RGPPPLLLFL GAALVLASGA VPAREAGSAV EAEELVKGSP    50
    AWEPPANDTR EEAGPPAAGE DEASWTAPGG ELAGPEEVLQ ESAAVTGTAW 100
    LEADSPGLGG VTAEAGSGDA QALPATLQAP HEVLGQSIMP PAIPEATEAS 150
    GPPSPTPGDK LSPASELPKE SPLEVWLNLG GSTPDPQGPE LTYPFQGTLE 200
    PQPASDIIDI DYFEGLDGEG RGADLGSFPG SPGTSENHPD TEGETPSWSL 250
    LDLYDDFTPF DESDFYPTTS FYDDLDEEEE EEEDDKDAVG GGDLEDENEL 300
    LVPTGKPGLG PGTGQPTSRW HAVPPQHTLG SVPGSSIALR PRPGEPGRDL 350
    ASSENGTECR SGFVRHNGSC RSVCDLFPSY CHNGGQCYLV ENIGAFCRCN 400
    TQDYIWHKGM RCESIITDFQ VMCVAVGSAA LVLLLLFMMT VFFAKKLYLL 450
    KTENTKLRRT NKFRTPSELH NDNFSLSTIA EGSHPNVRKL CNTPRTSSPH 500
    ARALAHYDNV ICQDDPSAPH KIQEVLKSCL KEEESFNIQN SMSPKLEGGK 550
    GDQADLDVNC LQNNLT 566
    Length:566
    Mass (Da):60,016
    Last modified:May 5, 2009 - v3
    Checksum:iDD8DA045C1BE31E5
    GO
    Isoform 2 (identifier: O95196-2) [UniParc]FASTAAdd to Basket

    Also known as: CSPG5-I

    The sequence of this isoform differs from the canonical sequence as follows:
         487-513: Missing.

    Show »
    Length:539
    Mass (Da):56,983
    Checksum:iBBD8B64B93091DDF
    GO
    Isoform 3 (identifier: O95196-3) [UniParc]FASTAAdd to Basket

    Also known as: CSPG5-II

    The sequence of this isoform differs from the canonical sequence as follows:
         1-138: Missing.
         487-513: Missing.

    Show »
    Length:401
    Mass (Da):43,621
    Checksum:i9F0D31F21854FCEB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti188 – 1881G → V.2 Publications
    Corresponds to variant rs3732530 [ dbSNP | Ensembl ].
    VAR_055089
    Natural varianti417 – 4171T → P.
    Corresponds to variant rs34016925 [ dbSNP | Ensembl ].
    VAR_055090

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 138138Missing in isoform 3. 1 PublicationVSP_015760Add
    BLAST
    Alternative sequencei487 – 51327Missing in isoform 2 and isoform 3. 2 PublicationsVSP_015761Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF059274 mRNA. Translation: AAC69612.1.
    AF461087 mRNA. Translation: AAQ04774.1.
    AF461088 mRNA. Translation: AAQ04775.1.
    AF461089 mRNA. Translation: AAQ04776.1.
    AC099778 Genomic DNA. No translation available.
    AC112512 Genomic DNA. No translation available.
    CCDSiCCDS2757.1. [O95196-2]
    CCDS56252.1. [O95196-3]
    CCDS56253.1. [O95196-1]
    RefSeqiNP_001193872.1. NM_001206943.1. [O95196-1]
    NP_001193873.1. NM_001206944.1.
    NP_006565.2. NM_006574.3. [O95196-2]
    UniGeneiHs.45127.

    Genome annotation databases

    EnsembliENST00000264723; ENSP00000264723; ENSG00000114646. [O95196-2]
    ENST00000383738; ENSP00000373244; ENSG00000114646. [O95196-1]
    ENST00000456150; ENSP00000392096; ENSG00000114646. [O95196-3]
    GeneIDi10675.
    KEGGihsa:10675.
    UCSCiuc003crn.3. human. [O95196-3]
    uc003cro.4. human. [O95196-2]
    uc003crp.4. human. [O95196-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF059274 mRNA. Translation: AAC69612.1 .
    AF461087 mRNA. Translation: AAQ04774.1 .
    AF461088 mRNA. Translation: AAQ04775.1 .
    AF461089 mRNA. Translation: AAQ04776.1 .
    AC099778 Genomic DNA. No translation available.
    AC112512 Genomic DNA. No translation available.
    CCDSi CCDS2757.1. [O95196-2 ]
    CCDS56252.1. [O95196-3 ]
    CCDS56253.1. [O95196-1 ]
    RefSeqi NP_001193872.1. NM_001206943.1. [O95196-1 ]
    NP_001193873.1. NM_001206944.1.
    NP_006565.2. NM_006574.3. [O95196-2 ]
    UniGenei Hs.45127.

    3D structure databases

    ProteinModelPortali O95196.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115917. 1 interaction.
    IntActi O95196. 1 interaction.
    STRINGi 9606.ENSP00000264723.

    PTM databases

    PhosphoSitei O95196.

    Proteomic databases

    PaxDbi O95196.
    PRIDEi O95196.

    Protocols and materials databases

    DNASUi 10675.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000264723 ; ENSP00000264723 ; ENSG00000114646 . [O95196-2 ]
    ENST00000383738 ; ENSP00000373244 ; ENSG00000114646 . [O95196-1 ]
    ENST00000456150 ; ENSP00000392096 ; ENSG00000114646 . [O95196-3 ]
    GeneIDi 10675.
    KEGGi hsa:10675.
    UCSCi uc003crn.3. human. [O95196-3 ]
    uc003cro.4. human. [O95196-2 ]
    uc003crp.4. human. [O95196-1 ]

    Organism-specific databases

    CTDi 10675.
    GeneCardsi GC03M047603.
    H-InvDB HIX0003276.
    HGNCi HGNC:2467. CSPG5.
    MIMi 606775. gene.
    neXtProti NX_O95196.
    PharmGKBi PA26965.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG39505.
    HOGENOMi HOG000112020.
    HOVERGENi HBG081361.
    InParanoidi O95196.
    KOi K08116.
    OMAi LEVNCLQ.
    OrthoDBi EOG7VX8WW.
    PhylomeDBi O95196.
    TreeFami TF338636.

    Enzyme and pathway databases

    Reactomei REACT_120800. Dermatan sulfate biosynthesis.
    REACT_120888. CS/DS degradation.
    REACT_120989. Chondroitin sulfate biosynthesis.
    REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.

    Miscellaneous databases

    GenomeRNAii 10675.
    NextBioi 40593.
    PROi O95196.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95196.
    Bgeei O95196.
    CleanExi HS_CSPG5.
    Genevestigatori O95196.

    Family and domain databases

    InterProi IPR010555. Chon_Sulph_att.
    IPR000742. EG-like_dom.
    IPR009505. Neural_ProG_Cyt.
    [Graphical view ]
    Pfami PF06566. Chon_Sulph_att. 1 hit.
    PF06567. Neural_ProG_Cyt. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and chromosomal mapping of the human gene of neuroglycan C (NGC), a neural transmembrane chondroitin sulfate proteoglycan with an EGF module."
      Yasuda Y., Tokita Y., Aono S., Matsui F., Ono T., Sonta S., Watanabe E., Nakanishi Y., Oohira A.
      Neurosci. Res. 32:313-322(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANT VAL-188.
      Tissue: Brain.
    2. "Expression of neuroglycan C (NGC), a transmembrane chondroitin sulfate proteoglycan with an EGF module, in the human brain."
      Aono S., Tokita Y., Yamauchi S., Shuo T., Matsui F., Yasuda Y., Keino H., Shimada A., Kishikawa M., Asai M., Oohira A.
      Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), VARIANT VAL-188.
    3. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "CALEB/NGC interacts with the Golgi-associated protein PIST."
      Hassel B., Schreff M., Stuebe E.-M., Blaich U., Schumacher S.
      J. Biol. Chem. 278:40136-40143(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GOPC.
    5. Cited for: INTERACTION WITH ERBB3, FUNCTION.
    6. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A., Ruetschi U., Larson G., Nilsson J.
      J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-165, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiCSPG5_HUMAN
    AccessioniPrimary (citable) accession number: O95196
    Secondary accession number(s): Q71M39, Q71M40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3