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O95196 (CSPG5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chondroitin sulfate proteoglycan 5
Alternative name(s):
Acidic leucine-rich EGF-like domain-containing brain protein
Neuroglycan C
Gene names
Name:CSPG5
Synonyms:CALEB, NGC
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation. Ref.5

Subunit structure

Binds TNR and probably TNC By similarity. Interacts with ERBB3 and GOPC. Ref.4 Ref.5

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Golgi apparatus membrane; Single-pass type I membrane protein By similarity. Note: In neurons, localizes to synaptic junctions By similarity. Also detected in the endoplasmic reticulum and the Golgi By similarity. Partially enriched in lipid rafts By similarity.

Tissue specificity

Restricted to brain (at protein level). Ref.1

Developmental stage

Expressed in brain of 3 months, 5 and 10-year-old individuals. Ref.1

Post-translational modification

N-glycosylated By similarity. Ref.6

O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation By similarity. Ref.6

Phosphorylated; in intracellular and extracellular parts By similarity.

Miscellaneous

Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage By similarity.

Sequence similarities

Contains 1 EGF-like domain.

Ontologies

Keywords
   Biological processDifferentiation
Growth regulation
Neurogenesis
   Cellular componentCell membrane
Endoplasmic reticulum
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
Proteoglycan
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon regeneration

Inferred from electronic annotation. Source: Ensembl

carbohydrate metabolic process

Traceable author statement. Source: Reactome

chondroitin sulfate biosynthetic process

Traceable author statement. Source: Reactome

chondroitin sulfate catabolic process

Traceable author statement. Source: Reactome

dermatan sulfate biosynthetic process

Traceable author statement. Source: Reactome

intracellular transport

Traceable author statement Ref.4. Source: UniProtKB

nervous system development

Traceable author statement Ref.4. Source: UniProtKB

regulation of growth

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of synaptic transmission

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi lumen

Traceable author statement. Source: Reactome

Golgi-associated vesicle membrane

Inferred from direct assay Ref.4. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Traceable author statement. Source: Reactome

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

lysosomal lumen

Traceable author statement. Source: Reactome

   Molecular_functiongrowth factor activity

Traceable author statement Ref.4. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GopcQ8BH603EBI-296349,EBI-296357From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95196-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95196-2)

Also known as: CSPG5-I;

The sequence of this isoform differs from the canonical sequence as follows:
     487-513: Missing.
Isoform 3 (identifier: O95196-3)

Also known as: CSPG5-II;

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.
     487-513: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 566536Chondroitin sulfate proteoglycan 5
PRO_0000042151

Regions

Topological domain31 – 423393Extracellular Potential
Transmembrane424 – 44421Helical; Potential
Topological domain445 – 566122Cytoplasmic Potential
Domain371 – 41343EGF-like
Region264 – 30138Interaction with TNC and TNR By similarity
Region442 – 46019Interaction with GOPC

Amino acid modifications

Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation1171O-linked (Xyl...) (chondroitin sulfate) By similarity
Glycosylation1651O-linked (GalNAc...) Ref.6
Disulfide bond374 ↔ 387 By similarity
Disulfide bond381 ↔ 397 By similarity
Disulfide bond399 ↔ 412 By similarity

Natural variations

Alternative sequence1 – 138138Missing in isoform 3.
VSP_015760
Alternative sequence487 – 51327Missing in isoform 2 and isoform 3.
VSP_015761
Natural variant1881G → V. Ref.1 Ref.2
Corresponds to variant rs3732530 [ dbSNP | Ensembl ].
VAR_055089
Natural variant4171T → P.
Corresponds to variant rs34016925 [ dbSNP | Ensembl ].
VAR_055090

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 3.
Checksum: DD8DA045C1BE31E5

FASTA56660,016
        10         20         30         40         50         60 
MGRAGGGGPG RGPPPLLLFL GAALVLASGA VPAREAGSAV EAEELVKGSP AWEPPANDTR 

        70         80         90        100        110        120 
EEAGPPAAGE DEASWTAPGG ELAGPEEVLQ ESAAVTGTAW LEADSPGLGG VTAEAGSGDA 

       130        140        150        160        170        180 
QALPATLQAP HEVLGQSIMP PAIPEATEAS GPPSPTPGDK LSPASELPKE SPLEVWLNLG 

       190        200        210        220        230        240 
GSTPDPQGPE LTYPFQGTLE PQPASDIIDI DYFEGLDGEG RGADLGSFPG SPGTSENHPD 

       250        260        270        280        290        300 
TEGETPSWSL LDLYDDFTPF DESDFYPTTS FYDDLDEEEE EEEDDKDAVG GGDLEDENEL 

       310        320        330        340        350        360 
LVPTGKPGLG PGTGQPTSRW HAVPPQHTLG SVPGSSIALR PRPGEPGRDL ASSENGTECR 

       370        380        390        400        410        420 
SGFVRHNGSC RSVCDLFPSY CHNGGQCYLV ENIGAFCRCN TQDYIWHKGM RCESIITDFQ 

       430        440        450        460        470        480 
VMCVAVGSAA LVLLLLFMMT VFFAKKLYLL KTENTKLRRT NKFRTPSELH NDNFSLSTIA 

       490        500        510        520        530        540 
EGSHPNVRKL CNTPRTSSPH ARALAHYDNV ICQDDPSAPH KIQEVLKSCL KEEESFNIQN 

       550        560 
SMSPKLEGGK GDQADLDVNC LQNNLT 

« Hide

Isoform 2 (CSPG5-I) [UniParc].

Checksum: BBD8B64B93091DDF
Show »

FASTA53956,983
Isoform 3 (CSPG5-II) [UniParc].

Checksum: 9F0D31F21854FCEB
Show »

FASTA40143,621

References

« Hide 'large scale' references
[1]"Cloning and chromosomal mapping of the human gene of neuroglycan C (NGC), a neural transmembrane chondroitin sulfate proteoglycan with an EGF module."
Yasuda Y., Tokita Y., Aono S., Matsui F., Ono T., Sonta S., Watanabe E., Nakanishi Y., Oohira A.
Neurosci. Res. 32:313-322(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANT VAL-188.
Tissue: Brain.
[2]"Expression of neuroglycan C (NGC), a transmembrane chondroitin sulfate proteoglycan with an EGF module, in the human brain."
Aono S., Tokita Y., Yamauchi S., Shuo T., Matsui F., Yasuda Y., Keino H., Shimada A., Kishikawa M., Asai M., Oohira A.
Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), VARIANT VAL-188.
[3]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"CALEB/NGC interacts with the Golgi-associated protein PIST."
Hassel B., Schreff M., Stuebe E.-M., Blaich U., Schumacher S.
J. Biol. Chem. 278:40136-40143(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GOPC.
[5]"Neuroglycan C, a novel member of the neuregulin family."
Kinugasa Y., Ishiguro H., Tokita Y., Oohira A., Ohmoto H., Higashiyama S.
Biochem. Biophys. Res. Commun. 321:1045-1049(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ERBB3, FUNCTION.
[6]"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
Halim A., Ruetschi U., Larson G., Nilsson J.
J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT SER-165, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF059274 mRNA. Translation: AAC69612.1.
AF461087 mRNA. Translation: AAQ04774.1.
AF461088 mRNA. Translation: AAQ04775.1.
AF461089 mRNA. Translation: AAQ04776.1.
AC099778 Genomic DNA. No translation available.
AC112512 Genomic DNA. No translation available.
RefSeqNP_001193872.1. NM_001206943.1.
NP_001193873.1. NM_001206944.1.
NP_006565.2. NM_006574.3.
UniGeneHs.45127.

3D structure databases

ProteinModelPortalO95196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115917. 1 interaction.
IntActO95196. 1 interaction.
STRING9606.ENSP00000264723.

PTM databases

PhosphoSiteO95196.

Proteomic databases

PaxDbO95196.
PRIDEO95196.

Protocols and materials databases

DNASU10675.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264723; ENSP00000264723; ENSG00000114646. [O95196-2]
ENST00000383738; ENSP00000373244; ENSG00000114646. [O95196-1]
ENST00000456150; ENSP00000392096; ENSG00000114646. [O95196-3]
GeneID10675.
KEGGhsa:10675.
UCSCuc003crn.3. human. [O95196-3]
uc003cro.4. human. [O95196-2]
uc003crp.4. human. [O95196-1]

Organism-specific databases

CTD10675.
GeneCardsGC03M047603.
H-InvDBHIX0003276.
HGNCHGNC:2467. CSPG5.
MIM606775. gene.
neXtProtNX_O95196.
PharmGKBPA26965.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39505.
HOGENOMHOG000112020.
HOVERGENHBG081361.
InParanoidO95196.
KOK08116.
OMALEVNCLQ.
OrthoDBEOG7VX8WW.
TreeFamTF338636.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.

Gene expression databases

ArrayExpressO95196.
BgeeO95196.
CleanExHS_CSPG5.
GenevestigatorO95196.

Family and domain databases

InterProIPR010555. Chon_Sulph_att.
IPR000742. EG-like_dom.
IPR009505. Neural_ProG_Cyt.
[Graphical view]
PfamPF06566. Chon_Sulph_att. 1 hit.
PF06567. Neural_ProG_Cyt. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi10675.
NextBio40593.
PROO95196.
SOURCESearch...

Entry information

Entry nameCSPG5_HUMAN
AccessionPrimary (citable) accession number: O95196
Secondary accession number(s): Q71M39, Q71M40
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: May 5, 2009
Last modified: March 19, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM