O95196 (CSPG5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chondroitin sulfate proteoglycan 5

Alternative name(s):
Acidic leucine-rich EGF-like domain-containing brain protein
Neuroglycan C

Gene names

Name:	CSPG5
Synonyms:	CALEB, NGC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation. Ref.5
Subunit structure	Binds TNR and probably TNC By similarity. Interacts with ERBB3 and GOPC. Ref.4 Ref.5
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Golgi apparatus membrane; Single-pass type I membrane protein By similarity. Note: In neurons, localizes to synaptic junctions By similarity. Also detected in the endoplasmic reticulum and the Golgi By similarity. Partially enriched in lipid rafts By similarity.
Tissue specificity	Restricted to brain (at protein level). Ref.1
Developmental stage	Expressed in brain of 3 months, 5 and 10-year-old individuals. Ref.1
Post-translational modification	N-glycosylated By similarity. Ref.6 O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation By similarity. Ref.6 Phosphorylated; in intracellular and extracellular parts By similarity.
Miscellaneous	Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage By similarity.
Sequence similarities	Contains 1 EGF-like domain.

Ontologies

Keywords
Biological process	Differentiation Growth regulation Neurogenesis
Cellular component	Cell membrane Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Phosphoprotein Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon regeneration Inferred from electronic annotation. Source: Compara carbohydrate metabolic process Traceable author statement. Source: Reactome chondroitin sulfate biosynthetic process Traceable author statement. Source: Reactome chondroitin sulfate catabolic process Traceable author statement. Source: Reactome dermatan sulfate biosynthetic process Traceable author statement. Source: Reactome intracellular transport Traceable author statement Ref.4. Source: UniProtKB nervous system development Traceable author statement Ref.4. Source: UniProtKB regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW regulation of synaptic transmission Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi lumen Traceable author statement. Source: Reactome Golgi-associated vesicle membrane Inferred from direct assay Ref.4. Source: UniProtKB endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Traceable author statement. Source: Reactome integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc lysosomal lumen Traceable author statement. Source: Reactome
Molecular_function	growth factor activity Traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gopc	Q8BH60	3	EBI-296349,EBI-296357	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95196-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95196-2)

Also known as: CSPG5-I;

The sequence of this isoform differs from the canonical sequence as follows:
     487-513: Missing.

Isoform 3 (identifier: O95196-3)

Also known as: CSPG5-II;

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.
     487-513: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 566	536	Chondroitin sulfate proteoglycan 5		PRO_0000042151
Regions
	Topological domain	31 – 423	393	Extracellular Potential
	Transmembrane	424 – 444	21	Helical; Potential
	Topological domain	445 – 566	122	Cytoplasmic Potential
	Domain	371 – 413	43	EGF-like
	Region	264 – 301	38	Interaction with TNC and TNR By similarity
	Region	442 – 460	19	Interaction with GOPC
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	117	1	O-linked (Xyl...) (chondroitin sulfate) By similarity
	Glycosylation	165	1	O-linked (GalNAc...) Ref.6
	Disulfide bond	374 ↔ 387		By similarity
	Disulfide bond	381 ↔ 397		By similarity
	Disulfide bond	399 ↔ 412		By similarity
Natural variations
	Alternative sequence	1 – 138	138	Missing in isoform 3.		VSP_015760
	Alternative sequence	487 – 513	27	Missing in isoform 2 and isoform 3.		VSP_015761
	Natural variant	188	1	G → V. Ref.1 Ref.2 Corresponds to variant rs3732530 [ dbSNP | Ensembl ].		VAR_055089
	Natural variant	417	1	T → P. Corresponds to variant rs34016925 [ dbSNP | Ensembl ].		VAR_055090

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 3. Checksum: DD8DA045C1BE31E5	FASTA	566	60,016
	10 20 30 40 50 60 MGRAGGGGPG RGPPPLLLFL GAALVLASGA VPAREAGSAV EAEELVKGSP AWEPPANDTR 70 80 90 100 110 120 EEAGPPAAGE DEASWTAPGG ELAGPEEVLQ ESAAVTGTAW LEADSPGLGG VTAEAGSGDA 130 140 150 160 170 180 QALPATLQAP HEVLGQSIMP PAIPEATEAS GPPSPTPGDK LSPASELPKE SPLEVWLNLG 190 200 210 220 230 240 GSTPDPQGPE LTYPFQGTLE PQPASDIIDI DYFEGLDGEG RGADLGSFPG SPGTSENHPD 250 260 270 280 290 300 TEGETPSWSL LDLYDDFTPF DESDFYPTTS FYDDLDEEEE EEEDDKDAVG GGDLEDENEL 310 320 330 340 350 360 LVPTGKPGLG PGTGQPTSRW HAVPPQHTLG SVPGSSIALR PRPGEPGRDL ASSENGTECR 370 380 390 400 410 420 SGFVRHNGSC RSVCDLFPSY CHNGGQCYLV ENIGAFCRCN TQDYIWHKGM RCESIITDFQ 430 440 450 460 470 480 VMCVAVGSAA LVLLLLFMMT VFFAKKLYLL KTENTKLRRT NKFRTPSELH NDNFSLSTIA 490 500 510 520 530 540 EGSHPNVRKL CNTPRTSSPH ARALAHYDNV ICQDDPSAPH KIQEVLKSCL KEEESFNIQN 550 560 SMSPKLEGGK GDQADLDVNC LQNNLT
	Isoform 2 (CSPG5-I) [UniParc]. Checksum: BBD8B64B93091DDF	FASTA	539	56,983
	10 20 30 40 50 60 MGRAGGGGPG RGPPPLLLFL GAALVLASGA VPAREAGSAV EAEELVKGSP AWEPPANDTR 70 80 90 100 110 120 EEAGPPAAGE DEASWTAPGG ELAGPEEVLQ ESAAVTGTAW LEADSPGLGG VTAEAGSGDA 130 140 150 160 170 180 QALPATLQAP HEVLGQSIMP PAIPEATEAS GPPSPTPGDK LSPASELPKE SPLEVWLNLG 190 200 210 220 230 240 GSTPDPQGPE LTYPFQGTLE PQPASDIIDI DYFEGLDGEG RGADLGSFPG SPGTSENHPD 250 260 270 280 290 300 TEGETPSWSL LDLYDDFTPF DESDFYPTTS FYDDLDEEEE EEEDDKDAVG GGDLEDENEL 310 320 330 340 350 360 LVPTGKPGLG PGTGQPTSRW HAVPPQHTLG SVPGSSIALR PRPGEPGRDL ASSENGTECR 370 380 390 400 410 420 SGFVRHNGSC RSVCDLFPSY CHNGGQCYLV ENIGAFCRCN TQDYIWHKGM RCESIITDFQ 430 440 450 460 470 480 VMCVAVGSAA LVLLLLFMMT VFFAKKLYLL KTENTKLRRT NKFRTPSELH NDNFSLSTIA 490 500 510 520 530 EGSHPNDDPS APHKIQEVLK SCLKEEESFN IQNSMSPKLE GGKGDQADLD VNCLQNNLT
	Isoform 3 (CSPG5-II) [UniParc]. Checksum: 9F0D31F21854FCEB	FASTA	401	43,621
	10 20 30 40 50 60 MPPAIPEATE ASGPPSPTPG DKLSPASELP KESPLEVWLN LGGSTPDPQG PELTYPFQGT 70 80 90 100 110 120 LEPQPASDII DIDYFEGLDG EGRGADLGSF PGSPGTSENH PDTEGETPSW SLLDLYDDFT 130 140 150 160 170 180 PFDESDFYPT TSFYDDLDEE EEEEEDDKDA VGGGDLEDEN ELLVPTGKPG LGPGTGQPTS 190 200 210 220 230 240 RWHAVPPQHT LGSVPGSSIA LRPRPGEPGR DLASSENGTE CRSGFVRHNG SCRSVCDLFP 250 260 270 280 290 300 SYCHNGGQCY LVENIGAFCR CNTQDYIWHK GMRCESIITD FQVMCVAVGS AALVLLLLFM 310 320 330 340 350 360 MTVFFAKKLY LLKTENTKLR RTNKFRTPSE LHNDNFSLST IAEGSHPNDD PSAPHKIQEV 370 380 390 400 LKSCLKEEES FNIQNSMSPK LEGGKGDQAD LDVNCLQNNL T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of the human gene of neuroglycan C (NGC), a neural transmembrane chondroitin sulfate proteoglycan with an EGF module." Yasuda Y., Tokita Y., Aono S., Matsui F., Ono T., Sonta S., Watanabe E., Nakanishi Y., Oohira A. Neurosci. Res. 32:313-322(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANT VAL-188. Tissue: Brain.
[2]	"Expression of neuroglycan C (NGC), a transmembrane chondroitin sulfate proteoglycan with an EGF module, in the human brain." Aono S., Tokita Y., Yamauchi S., Shuo T., Matsui F., Yasuda Y., Keino H., Shimada A., Kishikawa M., Asai M., Oohira A. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), VARIANT VAL-188.
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"CALEB/NGC interacts with the Golgi-associated protein PIST." Hassel B., Schreff M., Stuebe E.-M., Blaich U., Schumacher S. J. Biol. Chem. 278:40136-40143(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GOPC.
[5]	"Neuroglycan C, a novel member of the neuregulin family." Kinugasa Y., Ishiguro H., Tokita Y., Oohira A., Ohmoto H., Higashiyama S. Biochem. Biophys. Res. Commun. 321:1045-1049(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ERBB3, FUNCTION.
[6]	"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins." Halim A., Ruetschi U., Larson G., Nilsson J. J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT SER-165, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF059274 mRNA. Translation: AAC69612.1. AF461087 mRNA. Translation: AAQ04774.1. AF461088 mRNA. Translation: AAQ04775.1. AF461089 mRNA. Translation: AAQ04776.1. AC099778 Genomic DNA. No translation available. AC112512 Genomic DNA. No translation available.
IPI	IPI00008586. IPI00651663. IPI00651705.
RefSeq	NP_001193872.1. NM_001206943.1. NP_001193873.1. NM_001206944.1. NP_006565.2. NM_006574.3.
UniGene	Hs.45127.
3D structure databases
ProteinModelPortal	O95196.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95196. 1 interaction.
STRING	9606.ENSP00000264723.
PTM databases
PhosphoSite	O95196.
Proteomic databases
PaxDb	O95196.
PRIDE	O95196.
Protocols and materials databases
DNASU	10675.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264723; ENSP00000264723; ENSG00000114646. ENST00000383738; ENSP00000373244; ENSG00000114646. ENST00000456150; ENSP00000392096; ENSG00000114646.
GeneID	10675.
KEGG	hsa:10675.
UCSC	uc003crn.3. human. uc003cro.4. human. uc003crp.4. human.
Organism-specific databases
CTD	10675.
GeneCards	GC03M047603.
H-InvDB	HIX0003276.
HGNC	HGNC:2467. CSPG5.
MIM	606775. gene.
neXtProt	NX_O95196.
PharmGKB	PA26965.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39505.
HOGENOM	HOG000112020.
HOVERGEN	HBG081361.
InParanoid	O95196.
KO	K08116.
OMA	LEVNCLQ.
OrthoDB	EOG4XWFXN.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	O95196.
Bgee	O95196.
CleanEx	HS_CSPG5.
Genevestigator	O95196.
GermOnline	ENSG00000114646. Homo sapiens.
Family and domain databases
InterPro	IPR010555. Chon_Sulph_att. IPR000742. EG-like_dom. IPR009505. Neural_ProG_Cyt. [Graphical view]
Pfam	PF06566. Chon_Sulph_att. 1 hit. PF06567. Neural_ProG_Cyt. 1 hit. [Graphical view]
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. False negative. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10675.
NextBio	40593.
SOURCE	Search...

Entry information

Entry name

CSPG5_HUMAN

Accession

Primary (citable) accession number: O95196
Secondary accession number(s): Q71M39, Q71M40

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.