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Protein

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Gene

NDUFB8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

BioCyciZFISH:HS09335-MONOMER.
ReactomeiR-HSA-611105. Respiratory electron transport.
R-HSA-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
Alternative name(s):
Complex I-ASHI
Short name:
CI-ASHI
NADH-ubiquinone oxidoreductase ASHI subunit
Gene namesi
Name:NDUFB8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:7703. NDUFB8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4714.
OpenTargetsiENSG00000166136.
ENSG00000255339.
PharmGKBiPA31514.

Chemistry databases

ChEMBLiCHEMBL2363065.

Polymorphism and mutation databases

BioMutaiNDUFB8.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionBy similarityAdd BLAST28
ChainiPRO_000002004629 – 186NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrialAdd BLAST158

Proteomic databases

EPDiO95169.
MaxQBiO95169.
PaxDbiO95169.
PeptideAtlasiO95169.
PRIDEiO95169.
TopDownProteomicsiO95169-1. [O95169-1]

PTM databases

iPTMnetiO95169.
PhosphoSitePlusiO95169.
SwissPalmiO95169.

Expressioni

Gene expression databases

BgeeiENSG00000166136.
CleanExiHS_NDUFB8.
ExpressionAtlasiO95169. baseline and differential.
GenevisibleiO95169. HS.

Organism-specific databases

HPAiHPA003886.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.1 Publication

Protein-protein interaction databases

BioGridi110794. 62 interactors.
IntActiO95169. 23 interactors.
MINTiMINT-1371340.
STRINGi9606.ENSP00000299166.

Structurei

3D structure databases

ProteinModelPortaliO95169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFB8 subunit family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4040. Eukaryota.
ENOG4111WEY. LUCA.
GeneTreeiENSGT00390000000628.
HOGENOMiHOG000012999.
HOVERGENiHBG000931.
InParanoidiO95169.
KOiK03964.
OMAiIHWDLDM.
OrthoDBiEOG091G15KV.
PhylomeDBiO95169.
TreeFamiTF317319.

Family and domain databases

InterProiIPR008699. NDUFB8.
IPR016551. Ndufb8_metazoa.
[Graphical view]
PANTHERiPTHR12840. PTHR12840. 1 hit.
PfamiPF05821. NDUF_B8. 1 hit.
[Graphical view]
PIRSFiPIRSF009288. NDUB8. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95169-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVARAGVLG VQWLQRASRN VMPLGARTAS HMTKDMFPGP YPRTPEERAA
60 70 80 90 100
AAKKYNMRVE DYEPYPDDGM GYGDYPKLPD RSQHERDPWY SWDQPGLRLN
110 120 130 140 150
WGEPMHWHLD MYNRNRVDTS PTPVSWHVMC MQLFGFLAFM IFMCWVGDVY
160 170 180
PVYQPVGPKQ YPYNNLYLER GGDPSKEPER VVHYEI
Length:186
Mass (Da):21,766
Last modified:May 1, 1999 - v1
Checksum:i518459D44965D202
GO
Isoform 2 (identifier: O95169-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-172: GPKQYPYNNLYLERGG → CRHHFSYNLGFLSALG
     173-186: Missing.

Note: No experimental confirmation available.
Show »
Length:172
Mass (Da):20,039
Checksum:iD11BE3F6BEDD1658
GO
Isoform 3 (identifier: O95169-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available. Derived from EST data.
Show »
Length:155
Mass (Da):18,479
Checksum:iB409F622289FC308
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44T → I in CAB45691 (PubMed:11230166).Curated1
Sequence conflicti44T → I in CAG38521 (Ref. 5) Curated1
Sequence conflicti171G → S in AAD27761 (PubMed:11042152).Curated1
Sequence conflicti184Y → N in CAB45691 (PubMed:11230166).Curated1
Sequence conflicti184Y → N in CAG38521 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0548421 – 31Missing in isoform 3. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_054843157 – 172GPKQY…LERGG → CRHHFSYNLGFLSALG in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_054844173 – 186Missing in isoform 2. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044958 mRNA. Translation: AAD05422.1.
AF115968 mRNA. Translation: AAP97239.1.
AF077028 mRNA. Translation: AAD27761.1.
AL080056 mRNA. Translation: CAB45691.1.
CR533490 mRNA. Translation: CAG38521.1.
AK289579 mRNA. Translation: BAF82268.1.
AK295867 mRNA. Translation: BAG58666.1.
AL133352 Genomic DNA. Translation: CAH73563.1.
CH471066 Genomic DNA. Translation: EAW49819.1.
CH471066 Genomic DNA. Translation: EAW49820.1.
BC000466 mRNA. Translation: AAH00466.1.
BC019276 mRNA. Translation: AAH19276.1.
BI602529 mRNA. No translation available.
Y18944 Genomic DNA. Translation: CAB46274.1.
CCDSiCCDS65916.1. [O95169-3]
CCDS65917.1. [O95169-2]
CCDS7497.1. [O95169-1]
PIRiJE0382.
RefSeqiNP_001271296.1. NM_001284367.1. [O95169-2]
NP_001271297.1. NM_001284368.1. [O95169-3]
NP_004995.1. NM_005004.3. [O95169-1]
UniGeneiHs.523215.

Genome annotation databases

EnsembliENST00000299166; ENSP00000299166; ENSG00000166136. [O95169-1]
ENST00000370320; ENSP00000359344; ENSG00000166136. [O95169-2]
ENST00000370322; ENSP00000359346; ENSG00000166136. [O95169-3]
GeneIDi4714.
KEGGihsa:4714.
UCSCiuc001kri.3. human. [O95169-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044958 mRNA. Translation: AAD05422.1.
AF115968 mRNA. Translation: AAP97239.1.
AF077028 mRNA. Translation: AAD27761.1.
AL080056 mRNA. Translation: CAB45691.1.
CR533490 mRNA. Translation: CAG38521.1.
AK289579 mRNA. Translation: BAF82268.1.
AK295867 mRNA. Translation: BAG58666.1.
AL133352 Genomic DNA. Translation: CAH73563.1.
CH471066 Genomic DNA. Translation: EAW49819.1.
CH471066 Genomic DNA. Translation: EAW49820.1.
BC000466 mRNA. Translation: AAH00466.1.
BC019276 mRNA. Translation: AAH19276.1.
BI602529 mRNA. No translation available.
Y18944 Genomic DNA. Translation: CAB46274.1.
CCDSiCCDS65916.1. [O95169-3]
CCDS65917.1. [O95169-2]
CCDS7497.1. [O95169-1]
PIRiJE0382.
RefSeqiNP_001271296.1. NM_001284367.1. [O95169-2]
NP_001271297.1. NM_001284368.1. [O95169-3]
NP_004995.1. NM_005004.3. [O95169-1]
UniGeneiHs.523215.

3D structure databases

ProteinModelPortaliO95169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110794. 62 interactors.
IntActiO95169. 23 interactors.
MINTiMINT-1371340.
STRINGi9606.ENSP00000299166.

Chemistry databases

ChEMBLiCHEMBL2363065.

PTM databases

iPTMnetiO95169.
PhosphoSitePlusiO95169.
SwissPalmiO95169.

Polymorphism and mutation databases

BioMutaiNDUFB8.

Proteomic databases

EPDiO95169.
MaxQBiO95169.
PaxDbiO95169.
PeptideAtlasiO95169.
PRIDEiO95169.
TopDownProteomicsiO95169-1. [O95169-1]

Protocols and materials databases

DNASUi4714.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299166; ENSP00000299166; ENSG00000166136. [O95169-1]
ENST00000370320; ENSP00000359344; ENSG00000166136. [O95169-2]
ENST00000370322; ENSP00000359346; ENSG00000166136. [O95169-3]
GeneIDi4714.
KEGGihsa:4714.
UCSCiuc001kri.3. human. [O95169-1]

Organism-specific databases

CTDi4714.
DisGeNETi4714.
GeneCardsiNDUFB8.
HGNCiHGNC:7703. NDUFB8.
HPAiHPA003886.
MIMi602140. gene.
neXtProtiNX_O95169.
OpenTargetsiENSG00000166136.
ENSG00000255339.
PharmGKBiPA31514.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4040. Eukaryota.
ENOG4111WEY. LUCA.
GeneTreeiENSGT00390000000628.
HOGENOMiHOG000012999.
HOVERGENiHBG000931.
InParanoidiO95169.
KOiK03964.
OMAiIHWDLDM.
OrthoDBiEOG091G15KV.
PhylomeDBiO95169.
TreeFamiTF317319.

Enzyme and pathway databases

BioCyciZFISH:HS09335-MONOMER.
ReactomeiR-HSA-611105. Respiratory electron transport.
R-HSA-6799198. Complex I biogenesis.

Miscellaneous databases

GeneWikiiNDUFB8.
GenomeRNAii4714.
PROiO95169.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166136.
CleanExiHS_NDUFB8.
ExpressionAtlasiO95169. baseline and differential.
GenevisibleiO95169. HS.

Family and domain databases

InterProiIPR008699. NDUFB8.
IPR016551. Ndufb8_metazoa.
[Graphical view]
PANTHERiPTHR12840. PTHR12840. 1 hit.
PfamiPF05821. NDUF_B8. 1 hit.
[Graphical view]
PIRSFiPIRSF009288. NDUB8. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNDUB8_HUMAN
AccessioniPrimary (citable) accession number: O95169
Secondary accession number(s): A8K0L4
, Q5W143, Q5W144, Q5W145, Q9UG53, Q9UJR4, Q9UQF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.