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Protein

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Gene

NDUFB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: UniProtKB

GO - Biological processi

  • mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
  • mitochondrial respiratory chain complex I assembly Source: Reactome
  • response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

BioCyciZFISH:HS00843-MONOMER.
ReactomeiR-HSA-611105. Respiratory electron transport.
R-HSA-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
Alternative name(s):
Complex I-B15
Short name:
CI-B15
NADH-ubiquinone oxidoreductase B15 subunit
Gene namesi
Name:NDUFB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:7699. NDUFB4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei88 – 105HelicalSequence analysisAdd BLAST18

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: Reactome
  • mitochondrial respiratory chain complex I Source: UniProtKB
  • mitochondrion Source: UniProtKB
  • nuclear membrane Source: HPA
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4710.
OpenTargetsiENSG00000065518.
PharmGKBiPA31510.

Chemistry databases

ChEMBLiCHEMBL2363065.

Polymorphism and mutation databases

BioMutaiNDUFB4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001188002 – 129NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4Add BLAST128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei26PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO95168.
MaxQBiO95168.
PaxDbiO95168.
PeptideAtlasiO95168.
PRIDEiO95168.
TopDownProteomicsiO95168-1. [O95168-1]

PTM databases

iPTMnetiO95168.
PhosphoSitePlusiO95168.
SwissPalmiO95168.

Expressioni

Gene expression databases

BgeeiENSG00000065518.
CleanExiHS_NDUFB4.
ExpressionAtlasiO95168. baseline and differential.
GenevisibleiO95168. HS.

Organism-specific databases

HPAiHPA051739.
HPA064441.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.1 Publication

Protein-protein interaction databases

BioGridi110790. 40 interactors.
IntActiO95168. 25 interactors.
STRINGi9606.ENSP00000184266.

Structurei

3D structure databases

ProteinModelPortaliO95168.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFB4 subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZUV. Eukaryota.
ENOG4112CXK. LUCA.
GeneTreeiENSGT00390000007133.
HOGENOMiHOG000060241.
HOVERGENiHBG001159.
InParanoidiO95168.
KOiK03960.
OMAiRWVYARA.
OrthoDBiEOG091G1578.
PhylomeDBiO95168.
TreeFamiTF328761.

Family and domain databases

InterProiIPR009866. NADH_UbQ_OxRdtase_NDUFB4_su.
[Graphical view]
PANTHERiPTHR15469. PTHR15469. 1 hit.
PfamiPF07225. NDUF_B4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95168-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ
60 70 80 90 100
YNDPNRRGLI ENPALLRWAY ARTINVYPNF RPTPKNSLMG ALCGFGPLIF
110 120
IYYIIKTERD RKEKLIQEGK LDRTFHLSY
Length:129
Mass (Da):15,209
Last modified:January 23, 2007 - v3
Checksum:iE5060D0BF46FFF28
GO
Isoform 2 (identifier: O95168-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-129: DRKEKLIQEGKLDRTFHLSY → VSIQTRCLVFE

Note: No experimental confirmation available.
Show »
Length:120
Mass (Da):14,026
Checksum:i08BB084685E3098E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042719110 – 129DRKEK…FHLSY → VSIQTRCLVFE in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044957 mRNA. Translation: AAD05421.1.
AC126182 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79525.1.
BC000855 mRNA. Translation: AAH00855.1.
BC070285 mRNA. Translation: AAH70285.1.
BC146885 mRNA. Translation: AAI46886.1.
BC146887 mRNA. Translation: AAI46888.1.
BC146927 mRNA. Translation: AAI46928.1.
BC146932 mRNA. Translation: AAI46933.1.
CCDSiCCDS2999.1. [O95168-1]
CCDS54630.1. [O95168-2]
PIRiJE0383.
RefSeqiNP_001161803.1. NM_001168331.1. [O95168-2]
NP_004538.2. NM_004547.5. [O95168-1]
UniGeneiHs.304613.

Genome annotation databases

EnsembliENST00000184266; ENSP00000184266; ENSG00000065518. [O95168-1]
ENST00000485064; ENSP00000419578; ENSG00000065518. [O95168-2]
GeneIDi4710.
KEGGihsa:4710.
UCSCiuc003edt.4. human. [O95168-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044957 mRNA. Translation: AAD05421.1.
AC126182 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79525.1.
BC000855 mRNA. Translation: AAH00855.1.
BC070285 mRNA. Translation: AAH70285.1.
BC146885 mRNA. Translation: AAI46886.1.
BC146887 mRNA. Translation: AAI46888.1.
BC146927 mRNA. Translation: AAI46928.1.
BC146932 mRNA. Translation: AAI46933.1.
CCDSiCCDS2999.1. [O95168-1]
CCDS54630.1. [O95168-2]
PIRiJE0383.
RefSeqiNP_001161803.1. NM_001168331.1. [O95168-2]
NP_004538.2. NM_004547.5. [O95168-1]
UniGeneiHs.304613.

3D structure databases

ProteinModelPortaliO95168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110790. 40 interactors.
IntActiO95168. 25 interactors.
STRINGi9606.ENSP00000184266.

Chemistry databases

ChEMBLiCHEMBL2363065.

PTM databases

iPTMnetiO95168.
PhosphoSitePlusiO95168.
SwissPalmiO95168.

Polymorphism and mutation databases

BioMutaiNDUFB4.

Proteomic databases

EPDiO95168.
MaxQBiO95168.
PaxDbiO95168.
PeptideAtlasiO95168.
PRIDEiO95168.
TopDownProteomicsiO95168-1. [O95168-1]

Protocols and materials databases

DNASUi4710.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000184266; ENSP00000184266; ENSG00000065518. [O95168-1]
ENST00000485064; ENSP00000419578; ENSG00000065518. [O95168-2]
GeneIDi4710.
KEGGihsa:4710.
UCSCiuc003edt.4. human. [O95168-1]

Organism-specific databases

CTDi4710.
DisGeNETi4710.
GeneCardsiNDUFB4.
HGNCiHGNC:7699. NDUFB4.
HPAiHPA051739.
HPA064441.
MIMi603840. gene.
neXtProtiNX_O95168.
OpenTargetsiENSG00000065518.
PharmGKBiPA31510.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IZUV. Eukaryota.
ENOG4112CXK. LUCA.
GeneTreeiENSGT00390000007133.
HOGENOMiHOG000060241.
HOVERGENiHBG001159.
InParanoidiO95168.
KOiK03960.
OMAiRWVYARA.
OrthoDBiEOG091G1578.
PhylomeDBiO95168.
TreeFamiTF328761.

Enzyme and pathway databases

BioCyciZFISH:HS00843-MONOMER.
ReactomeiR-HSA-611105. Respiratory electron transport.
R-HSA-6799198. Complex I biogenesis.

Miscellaneous databases

ChiTaRSiNDUFB4. human.
GenomeRNAii4710.
PROiO95168.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065518.
CleanExiHS_NDUFB4.
ExpressionAtlasiO95168. baseline and differential.
GenevisibleiO95168. HS.

Family and domain databases

InterProiIPR009866. NADH_UbQ_OxRdtase_NDUFB4_su.
[Graphical view]
PANTHERiPTHR15469. PTHR15469. 1 hit.
PfamiPF07225. NDUF_B4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUB4_HUMAN
AccessioniPrimary (citable) accession number: O95168
Secondary accession number(s): B2RUY3, B9EJC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.