O95168 (NDUB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Alternative name(s):
Complex I-B15
Short name=CI-B15
NADH-ubiquinone oxidoreductase B15 subunit

Gene names

Name:

NDUFB4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Subunit structure	Complex I is composed of 45 different subunits.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I NDUFB4 subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Non-traceable author statement Ref.1. Source: UniProtKB response to oxidative stress Inferred from direct assay. Source: UniProtKB transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex I Inferred from direct assay Ref.4. Source: UniProtKB
Molecular function	NADH dehydrogenase (ubiquinone) activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 129	128	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		PRO_0000118800
Regions
	Transmembrane	88 – 105	18	Helical; Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O95168 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E5060D0BF46FFF28	FASTA	129	15,209
	10 20 30 40 50 60 MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ YNDPNRRGLI 70 80 90 100 110 120 ENPALLRWAY ARTINVYPNF RPTPKNSLMG ALCGFGPLIF IYYIIKTERD RKEKLIQEGK LDRTFHLSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed." Loeffen J.L.C.M., Triepels R.H., van den Heuvel L.P., Schuelke M., Buskens C.A.F., Smeets R.J.P., Trijbels J.M.F., Smeitink J.A.M. Biochem. Biophys. Res. Commun. 253:415-422(1998) [PubMed: 9878551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Cervix.
[4]	"The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification." Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F., Ghosh S.S., Capaldi R.A. J. Biol. Chem. 278:13619-13622(2003) [PubMed: 12611891] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044957 mRNA. Translation: AAD05421.1. CH471052 Genomic DNA. Translation: EAW79525.1. BC000855 mRNA. Translation: AAH00855.1. BC070285 mRNA. Translation: AAH70285.1. BC146885 mRNA. Translation: AAI46886.1. BC146887 mRNA. Translation: AAI46888.1.
IPI	IPI00220059.
PIR	JE0383.
RefSeq	NP_001161803.1. NM_001168331.1. NP_004538.2. NM_004547.5.
UniGene	Hs.304613.
3D structure databases
ProteinModelPortal	O95168.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95168. 1 interaction.
STRING	O95168.
PTM databases
PhosphoSite	O95168.
Proteomic databases
PRIDE	O95168.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000184266; ENSP00000184266; ENSG00000065518.
GeneID	4710.
KEGG	hsa:4710.
UCSC	uc003edu.1. human.
Organism-specific databases
CTD	4710.
GeneCards	GC03P120315.
H-InvDB	HIX0057358.
HGNC	HGNC:7699. NDUFB4.
HPA	CAB034930.
MIM	603840. gene.
neXtProt	NX_O95168.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20509.
GeneTree	ENSGT00390000007133.
HOGENOM	HBG278796.
HOVERGEN	HBG001159.
InParanoid	O95168.
OMA	EYDISPE.
OrthoDB	EOG49KFRZ.
PhylomeDB	O95168.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O95168.
Bgee	O95168.
CleanEx	HS_NDUFB4.
Genevestigator	O95168.
GermOnline	ENSG00000065518. Homo sapiens.
Family and domain databases
InterPro	IPR009866. NADH_UbQ_OxRdtase_NDUFB4_su. [Graphical view]
KO	K03960.
PANTHER	PTHR15469. NDUFB4. 1 hit.
Pfam	PF07225. NDUF_B4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00157. NADH.
NextBio	18164.
SOURCE	Search...

Entry information

Entry name

NDUB4_HUMAN

Accession

Primary (citable) accession number: O95168
Secondary accession number(s): B9EJC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.