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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

Gene

NDUFA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB

GO - Biological processi

  1. cellular metabolic process Source: Reactome
  2. mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
  3. respiratory electron transport chain Source: Reactome
  4. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiREACT_22393. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
Alternative name(s):
Complex I-B9
Short name:
CI-B9
NADH-ubiquinone oxidoreductase B9 subunit
Gene namesi
Name:NDUFA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componentsi: Chromosome 19, Unplaced

Organism-specific databases

HGNCiHGNC:7686. NDUFA3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: Reactome
  3. mitochondrial respiratory chain complex I Source: UniProtKB
  4. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31492.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 8483NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3PRO_0000118793Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO95167.
PeptideAtlasiO95167.
PRIDEiO95167.

PTM databases

PhosphoSiteiO95167.

Expressioni

Gene expression databases

BgeeiO95167.
CleanExiHS_NDUFA3.
ExpressionAtlasiO95167. baseline and differential.
GenevestigatoriO95167.

Organism-specific databases

HPAiHPA046976.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.1 Publication

Protein-protein interaction databases

BioGridi110776. 9 interactions.
STRINGi9606.ENSP00000398290.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFA3 subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46010.
HOGENOMiHOG000015319.
HOVERGENiHBG006495.
InParanoidiO95167.
KOiK03947.
OMAiKYSSMIN.
PhylomeDBiO95167.
TreeFamiTF333021.

Family and domain databases

InterProiIPR026626. NDUFA3.
[Graphical view]
PANTHERiPTHR15221. PTHR15221. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARVGAFLK NAWDKEPVLV VSFVVGGLAV ILPPLSPYFK YSVMINKATP
60 70 80
YNYPVPVRDD GNMPDVPSHP QDPQGPSLEW LKKL
Length:84
Mass (Da):9,279
Last modified:April 30, 1999 - v1
Checksum:i38B27A96D7A05D31
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti62 – 621N → D in a breast cancer sample; somatic mutation. 1 Publication
VAR_036175

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044955 mRNA. Translation: AAD05420.1.
AF070653 mRNA. Translation: AAD20959.1.
BC022369 mRNA. Translation: AAH22369.1.
BC061644 mRNA. Translation: AAH61644.1.
CCDSiCCDS12877.1.
PIRiJE0379.
RefSeqiNP_004533.1. NM_004542.3.
UniGeneiHs.198269.

Genome annotation databases

EnsembliENST00000419113; ENSP00000398290; ENSG00000170906.
ENST00000485876; ENSP00000418438; ENSG00000170906.
ENST00000610406; ENSP00000478119; ENSG00000275724.
ENST00000612327; ENSP00000480437; ENSG00000276220.
ENST00000613276; ENSP00000477919; ENSG00000273642.
ENST00000617282; ENSP00000482015; ENSG00000278365.
ENST00000618338; ENSP00000481545; ENSG00000274359.
ENST00000619253; ENSP00000483934; ENSG00000275605.
ENST00000619966; ENSP00000484436; ENSG00000276766.
ENST00000620289; ENSP00000477783; ENSG00000276061.
ENST00000621307; ENSP00000483002; ENSG00000277722.
ENST00000622523; ENSP00000483683; ENSG00000276061.
GeneIDi4696.
KEGGihsa:4696.
UCSCiuc002qde.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044955 mRNA. Translation: AAD05420.1.
AF070653 mRNA. Translation: AAD20959.1.
BC022369 mRNA. Translation: AAH22369.1.
BC061644 mRNA. Translation: AAH61644.1.
CCDSiCCDS12877.1.
PIRiJE0379.
RefSeqiNP_004533.1. NM_004542.3.
UniGeneiHs.198269.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110776. 9 interactions.
STRINGi9606.ENSP00000398290.

Chemistry

ChEMBLiCHEMBL2363065.

PTM databases

PhosphoSiteiO95167.

Proteomic databases

PaxDbiO95167.
PeptideAtlasiO95167.
PRIDEiO95167.

Protocols and materials databases

DNASUi4696.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000419113; ENSP00000398290; ENSG00000170906.
ENST00000485876; ENSP00000418438; ENSG00000170906.
ENST00000610406; ENSP00000478119; ENSG00000275724.
ENST00000612327; ENSP00000480437; ENSG00000276220.
ENST00000613276; ENSP00000477919; ENSG00000273642.
ENST00000617282; ENSP00000482015; ENSG00000278365.
ENST00000618338; ENSP00000481545; ENSG00000274359.
ENST00000619253; ENSP00000483934; ENSG00000275605.
ENST00000619966; ENSP00000484436; ENSG00000276766.
ENST00000620289; ENSP00000477783; ENSG00000276061.
ENST00000621307; ENSP00000483002; ENSG00000277722.
ENST00000622523; ENSP00000483683; ENSG00000276061.
GeneIDi4696.
KEGGihsa:4696.
UCSCiuc002qde.3. human.

Organism-specific databases

CTDi4696.
GeneCardsiGC19P054590.
HGNCiHGNC:7686. NDUFA3.
HPAiHPA046976.
MIMi603832. gene.
neXtProtiNX_O95167.
PharmGKBiPA31492.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG46010.
HOGENOMiHOG000015319.
HOVERGENiHBG006495.
InParanoidiO95167.
KOiK03947.
OMAiKYSSMIN.
PhylomeDBiO95167.
TreeFamiTF333021.

Enzyme and pathway databases

ReactomeiREACT_22393. Respiratory electron transport.

Miscellaneous databases

GenomeRNAii4696.
NextBioi18110.
PROiO95167.
SOURCEiSearch...

Gene expression databases

BgeeiO95167.
CleanExiHS_NDUFA3.
ExpressionAtlasiO95167. baseline and differential.
GenevestigatoriO95167.

Family and domain databases

InterProiIPR026626. NDUFA3.
[Graphical view]
PANTHERiPTHR15221. PTHR15221. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed."
    Loeffen J.L.C.M., Triepels R.H., van den Heuvel L.P., Schuelke M., Buskens C.A.F., Smeets R.J.P., Trijbels J.M.F., Smeitink J.A.M.
    Biochem. Biophys. Res. Commun. 253:415-422(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas and Prostate.
  4. "The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification."
    Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F., Ghosh S.S., Capaldi R.A.
    J. Biol. Chem. 278:13619-13622(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: VARIANT [LARGE SCALE ANALYSIS] ASP-62.

Entry informationi

Entry nameiNDUA3_HUMAN
AccessioniPrimary (citable) accession number: O95167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1999
Last sequence update: April 30, 1999
Last modified: March 3, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.