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O95166 (GBRAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
GABA(A) receptor-associated protein
MM46
Gene names
Name:GABARAP
Synonyms:FLC3B
ORF Names:HT004
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length117 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy By similarity. Ref.6

Subunit structure

Interacts with GPHN and NSF By similarity. Interacts with GABRG2, beta-tubulin and ULK1. Interacts with CALR. Interacts with DDX47. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 Ref.12 Ref.13 Ref.17

Subcellular location

Endomembrane system By similarity. Cytoplasmcytoskeleton By similarity. Golgi apparatus membrane By similarity. Cytoplasmic vesicleautophagosome. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules By similarity. Ref.7 Ref.8

Tissue specificity

Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Ref.1 Ref.2

Sequence similarities

Belongs to the MAP1 LC3 family.

Ontologies

Keywords
   Biological processApoptosis
Autophagy
Protein transport
Transport
   Cellular componentCytoplasm
Cytoplasmic vesicle
Cytoskeleton
Golgi apparatus
Membrane
Microtubule
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from direct assay Ref.6. Source: UniProtKB

autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule cytoskeleton organization

Inferred from electronic annotation. Source: Compara

protein targeting

Traceable author statement Ref.1. Source: ProtInc

synaptic transmission

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

actin cytoskeleton

Inferred from electronic annotation. Source: Compara

autophagic vacuole membrane

Inferred from direct assay PubMed 15169837. Source: UniProtKB

cell body

Inferred from electronic annotation. Source: Compara

cytoplasmic vesicle

Inferred from electronic annotation. Source: UniProtKB-KW

lysosome

Inferred from electronic annotation. Source: Compara

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule associated complex

Inferred from electronic annotation. Source: Compara

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Compara

plasma membrane

Traceable author statement Ref.1. Source: ProtInc

smooth endoplasmic reticulum

Inferred from electronic annotation. Source: Compara

   Molecular_functionbeta-tubulin binding

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 117117Gamma-aminobutyric acid receptor-associated protein
PRO_0000212363

Regions

Region1 – 2222Interaction with beta-tubulin
Region36 – 11782Interaction with GPHN By similarity
Region36 – 6833Interaction with GABRG2 Potential

Secondary structure

.................... 117
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95166 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: BC0B84B8A51C1E32

FASTA11713,918
        10         20         30         40         50         60 
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF 

        70         80         90        100        110 
YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL 

« Hide

References

« Hide 'large scale' references
[1]"GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton."
Wang H., Bedford F.K., Brandon N.J., Moss S.J., Olsen R.W.
Nature 397:69-72(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GABRG2 AND BETA-TUBULIN.
Tissue: Brain.
[2]"Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation."
Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A.
Brain Res. Mol. Brain Res. 85:1-12(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ULK1.
Tissue: Frontal cortex.
[3]Iijima M., Mitsui Y.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]Ye M., Fu G., Wu J., Zhou J., Zhang Q., Shen Y., Kan L., He K., Gu B., Chen S., Mao M., Chen Z.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. expand/collapse author list , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hypothalamus.
[6]"GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)."
Lee J.H., Rho S.B., Chun T.
Biotechnol. Lett. 27:623-628(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH DDX47.
[7]"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy."
Pankiv S., Clausen T.H., Lamark T., Brech A., Bruun J.A., Outzen H., Overvatn A., Bjorkoy G., Johansen T.
J. Biol. Chem. 282:24131-24145(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
[8]"The TP53INP2 protein is required for autophagy in mammalian cells."
Nowak J., Archange C., Tardivel-Lacombe J., Pontarotti P., Pebusque M.J., Vaccaro M.I., Velasco G., Dagorn J.C., Iovanna J.L.
Mol. Biol. Cell 20:870-881(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TP53INP2.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation."
Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M.
J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TBC1D25.
[11]"TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death."
Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J.
Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TP53INP1.
[12]"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription."
Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A.
PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TP53INP1 AND TP53INP2.
[13]"The X-ray crystal structure and putative ligand-derived peptide binding properties of gamma-aminobutyric acid receptor type A receptor-associated protein."
Knight D., Harris R., McAlister M.S.B., Phelan J.P., Geddes S., Moss S.J., Driscoll P.C., Keep N.H.
J. Biol. Chem. 277:5556-5561(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), INTERACTION WITH GABRG2; TUBULIN ALPHA AND TUBULIN BETA.
[14]"Solution structure of human GABA(A) receptor-associated protein GABARAP: implications for biological function and its regulation."
Stangler T., Mayr L.M., Willbold D.
J. Biol. Chem. 277:13363-13366(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[15]"1H, 13C and '5N resonance assignments of GABARAP, GABAA receptor associated protein."
Kouno T., Miura K., Kanematsu T., Shirakawa M., Hirata M., Kawano K.
J. Biomol. NMR 22:97-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[16]"Ligand binding mode of GABAA receptor-associated protein."
Weiergraber O.H., Stangler T., Thielmann Y., Mohrluder J., Wiesehan K., Willbold D.
J. Mol. Biol. 381:1320-1331(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS).
[17]"Structural framework of the GABARAP-calreticulin interface --implications for substrate binding to endoplasmic reticulum chaperones."
Thielmann Y., Weiergraber O.H., Mohrluder J., Willbold D.
FEBS J. 276:1140-1152(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH CALR, INTERACTION WITH CALR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF161586 mRNA. Translation: AAD47641.1.
AB030711 mRNA. Translation: BAB21549.1.
AF044671 mRNA. Translation: AAD02337.1.
AF067171 mRNA. Translation: AAD32455.1.
AF183425 mRNA. Translation: AAG09694.1.
IPIIPI00027253.
RefSeqNP_009209.1. NM_007278.1.
UniGeneHs.647421.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GNUX-ray1.75A1-117[»]
1KLVNMR-A1-117[»]
1KM7NMR-A1-117[»]
1KOTNMR-A1-117[»]
3D32X-ray1.30A/B1-117[»]
3DOWX-ray2.30A1-117[»]
ProteinModelPortalO95166.
ModBaseSearch...

Protein-protein interaction databases

IntActO95166. 471 interactions.
MINTMINT-206256.
STRING9606.ENSP00000306866.

Protein family/group databases

TCDB1.A.9.5.2. neurotransmitter receptor, cys loop, ligand-gated ion channel (LIC) family.

PTM databases

PhosphoSiteO95166.

Proteomic databases

PaxDbO95166.
PRIDEO95166.

Protocols and materials databases

DNASU11337.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000302386; ENSP00000306866; ENSG00000170296.
GeneID11337.
KEGGhsa:11337.
UCSCuc002gfb.3. human.

Organism-specific databases

CTD11337.
GeneCardsGC17M007143.
HGNCHGNC:4067. GABARAP.
MIM605125. gene.
neXtProtNX_O95166.
PharmGKBPA28480.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG263746.
HOGENOMHOG000232034.
HOVERGENHBG051706.
InParanoidO95166.
KOK08341.
OMATTMGQLY.
OrthoDBEOG4T4CWW.

Gene expression databases

ArrayExpressO95166.
BgeeO95166.
CleanExHS_GABARAP.
GenevestigatorO95166.
GermOnlineENSG00000170296. Homo sapiens.

Family and domain databases

InterProIPR004241. Atg8_ubiquitin-like.
[Graphical view]
PANTHERPTHR10969. PTHR10969. 1 hit.
PfamPF02991. Atg8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGABARAP. human.
EvolutionaryTraceO95166.
GenomeRNAi11337.
NextBio43075.
PMAP-CutDBO95166.
SOURCESearch...

Entry information

Entry nameGBRAP_HUMAN
AccessionPrimary (citable) accession number: O95166
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 1999
Last modified: May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families