Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Gamma-aminobutyric acid receptor-associated protein

Gene

GABARAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.1 Publication

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • chemical synaptic transmission Source: ProtInc
  • extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  • macroautophagy Source: Reactome
  • microtubule cytoskeleton organization Source: Ensembl
  • protein targeting Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170296-MONOMER.
ReactomeiR-HSA-1632852. Macroautophagy.
SIGNORiO95166.

Protein family/group databases

TCDBi1.A.9.5.2. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
GABA(A) receptor-associated protein
MM46
Gene namesi
Name:GABARAP
Synonyms:FLC3B
ORF Names:HT004
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:4067. GABARAP.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: Ensembl
  • autophagosome Source: UniProtKB
  • autophagosome membrane Source: UniProtKB
  • axoneme Source: UniProtKB
  • cell body Source: Ensembl
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: Reactome
  • Golgi membrane Source: UniProtKB-SubCell
  • lysosome Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • microtubule associated complex Source: Ensembl
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: ProtInc
  • smooth endoplasmic reticulum Source: Ensembl
  • sperm midpiece Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49 – 50YL → AA: Inhibits interaction with TECPR2. 1 Publication2
Mutagenesisi67R → A: No effect on interaction with TECPR2. 1 Publication1
Mutagenesisi116G → A: Impairs localization at the autophagosomal membrane. 1 Publication1

Organism-specific databases

DisGeNETi11337.
OpenTargetsiENSG00000170296.
PharmGKBiPA28480.

Polymorphism and mutation databases

BioMutaiGABARAP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123631 – 116Gamma-aminobutyric acid receptor-associated proteinAdd BLAST116
PropeptideiPRO_0000423065117Removed in mature form1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi116Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116 – 117Cleavage; by ATG4B2

Keywords - PTMi

Lipoprotein

Proteomic databases

EPDiO95166.
PaxDbiO95166.
PeptideAtlasiO95166.
PRIDEiO95166.

PTM databases

iPTMnetiO95166.
PhosphoSitePlusiO95166.

Miscellaneous databases

PMAP-CutDBO95166.

Expressioni

Tissue specificityi

Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.2 Publications

Gene expression databases

BgeeiENSG00000170296.
CleanExiHS_GABARAP.
ExpressionAtlasiO95166. baseline and differential.
GenevisibleiO95166. HS.

Interactioni

Subunit structurei

Interacts with GPHN and NSF (By similarity). Interacts with ATG3, ATG7, ATG13, RB1CC1, GABRG2, beta-tubulin and ULK1 (PubMed:11825910, PubMed:12507496, PubMed:11096062, PubMed:23043107, PubMed:11146101, PubMed:9892355, PubMed:11729197). Interacts with CALR (PubMed:19154346). Interacts with DDX47 (PubMed:15977068). Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22470510, PubMed:22421968). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1 (PubMed:17580304). Interacts with MAPK15 (PubMed:22948227). Interacts with TECPR2 and PCM1 (PubMed:20562859, PubMed:24089205). Interacts with TBC1D5 (PubMed:22354992). Interacts with TRIM5 (PubMed:25127057). Interacts with MEFV and TRIM21 (PubMed:26347139). Interacts with KIF21B (By similarity).By similarity20 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG2AQ2TAZ02EBI-712001,EBI-2514077
ATG3Q9NT625EBI-712001,EBI-988094
ATG4BQ9Y4P18EBI-712001,EBI-712014
ATG5Q9H1Y02EBI-712001,EBI-1047414
ATG7O953528EBI-712001,EBI-987834
Bnip3lQ9Z2F72EBI-712001,EBI-1774669From a different organism.
CALRP277974EBI-712001,EBI-1049597
DDX47Q9H0S43EBI-712001,EBI-2515241
DYX1C1Q8WXU24EBI-712001,EBI-2946907
FNBP1Q96RU32EBI-712001,EBI-1111248
FNIP1Q8TF405EBI-712001,EBI-2946919
FYCO1Q9BQS82EBI-712001,EBI-2869338
GBASO753235EBI-712001,EBI-307133
HADHAP409395EBI-712001,EBI-356720
IPO5O004106EBI-712001,EBI-356424
KBTBD6Q86V973EBI-712001,EBI-2514778
KBTBD7Q8WVZ92EBI-712001,EBI-473695
NBR1Q145965EBI-712001,EBI-742698
NCOA7Q8NI084EBI-712001,EBI-80799
NEDD4P469346EBI-712001,EBI-726944
NEK9Q8TD195EBI-712001,EBI-1044009
NSMAFQ926362EBI-712001,EBI-2947053
RASSF1Q9NS232EBI-712001,EBI-367363
RASSF5Q8WWW02EBI-712001,EBI-367390
SQSTM1Q1350110EBI-712001,EBI-307104
STBD1O952105EBI-712001,EBI-2947137
STK3Q131882EBI-712001,EBI-992580
STK4Q130432EBI-712001,EBI-367376
TBC1D15Q8TC075EBI-712001,EBI-1048247
TBC1D2BQ9UPU72EBI-712001,EBI-2947180
TECPR2O150402EBI-712001,EBI-2946991
UBA5Q9GZZ92EBI-712001,EBI-747805

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116465. 62 interactors.
DIPiDIP-35050N.
IntActiO95166. 475 interactors.
MINTiMINT-206256.
STRINGi9606.ENSP00000306866.

Structurei

Secondary structure

1117
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Helixi11 – 24Combined sources14
Beta strandi28 – 35Combined sources8
Beta strandi47 – 52Combined sources6
Helixi57 – 68Combined sources12
Beta strandi77 – 80Combined sources4
Beta strandi87 – 90Combined sources4
Helixi91 – 98Combined sources8
Beta strandi101 – 103Combined sources3
Beta strandi105 – 111Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNUX-ray1.75A1-117[»]
1KLVNMR-A1-117[»]
1KM7NMR-A1-117[»]
1KOTNMR-A1-117[»]
3D32X-ray1.30A/B1-117[»]
3DOWX-ray2.30A1-117[»]
3WIMX-ray2.60A1-117[»]
4XC2X-ray1.90A/B/C/D3-116[»]
5DPSX-ray2.00A/B/C2-117[»]
ProteinModelPortaliO95166.
SMRiO95166.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95166.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 22Interaction with beta-tubulinAdd BLAST22
Regioni36 – 117Interaction with GPHNBy similarityAdd BLAST82
Regioni36 – 68Interaction with GABRG2Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
KOiK08341.
OMAiTTMGQLY.
OrthoDBiEOG091G10QX.
PhylomeDBiO95166.
TreeFamiTF314556.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL
60 70 80 90 100
VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE
110
EDFFLYIAYS DESVYGL
Length:117
Mass (Da):13,918
Last modified:May 1, 1999 - v1
Checksum:iBC0B84B8A51C1E32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161586 mRNA. Translation: AAD47641.1.
AB030711 mRNA. Translation: BAB21549.1.
AF044671 mRNA. Translation: AAD02337.1.
AF067171 mRNA. Translation: AAD32455.1.
AF183425 mRNA. Translation: AAG09694.1.
CCDSiCCDS11092.1.
RefSeqiNP_009209.1. NM_007278.1.
UniGeneiHs.647421.

Genome annotation databases

EnsembliENST00000302386; ENSP00000306866; ENSG00000170296.
GeneIDi11337.
KEGGihsa:11337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161586 mRNA. Translation: AAD47641.1.
AB030711 mRNA. Translation: BAB21549.1.
AF044671 mRNA. Translation: AAD02337.1.
AF067171 mRNA. Translation: AAD32455.1.
AF183425 mRNA. Translation: AAG09694.1.
CCDSiCCDS11092.1.
RefSeqiNP_009209.1. NM_007278.1.
UniGeneiHs.647421.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNUX-ray1.75A1-117[»]
1KLVNMR-A1-117[»]
1KM7NMR-A1-117[»]
1KOTNMR-A1-117[»]
3D32X-ray1.30A/B1-117[»]
3DOWX-ray2.30A1-117[»]
3WIMX-ray2.60A1-117[»]
4XC2X-ray1.90A/B/C/D3-116[»]
5DPSX-ray2.00A/B/C2-117[»]
ProteinModelPortaliO95166.
SMRiO95166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116465. 62 interactors.
DIPiDIP-35050N.
IntActiO95166. 475 interactors.
MINTiMINT-206256.
STRINGi9606.ENSP00000306866.

Protein family/group databases

TCDBi1.A.9.5.2. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

PTM databases

iPTMnetiO95166.
PhosphoSitePlusiO95166.

Polymorphism and mutation databases

BioMutaiGABARAP.

Proteomic databases

EPDiO95166.
PaxDbiO95166.
PeptideAtlasiO95166.
PRIDEiO95166.

Protocols and materials databases

DNASUi11337.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302386; ENSP00000306866; ENSG00000170296.
GeneIDi11337.
KEGGihsa:11337.

Organism-specific databases

CTDi11337.
DisGeNETi11337.
GeneCardsiGABARAP.
HGNCiHGNC:4067. GABARAP.
MIMi605125. gene.
neXtProtiNX_O95166.
OpenTargetsiENSG00000170296.
PharmGKBiPA28480.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
KOiK08341.
OMAiTTMGQLY.
OrthoDBiEOG091G10QX.
PhylomeDBiO95166.
TreeFamiTF314556.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170296-MONOMER.
ReactomeiR-HSA-1632852. Macroautophagy.
SIGNORiO95166.

Miscellaneous databases

ChiTaRSiGABARAP. human.
EvolutionaryTraceiO95166.
GeneWikiiGABARAP.
GenomeRNAii11337.
PMAP-CutDBO95166.
PROiO95166.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170296.
CleanExiHS_GABARAP.
ExpressionAtlasiO95166. baseline and differential.
GenevisibleiO95166. HS.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGBRAP_HUMAN
AccessioniPrimary (citable) accession number: O95166
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.