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Protein

Gamma-aminobutyric acid receptor-associated protein

Gene

GABARAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.1 Publication

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • microtubule binding Source: Ensembl
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processApoptosis, Autophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1632852. Macroautophagy.
R-HSA-8854214. TBC/RABGAPs.
SIGNORiO95166.

Protein family/group databases

TCDBi1.A.9.5.2. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
GABA(A) receptor-associated protein
MM46
Gene namesi
Name:GABARAP
Synonyms:FLC3B
ORF Names:HT004
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:4067. GABARAP.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: Ensembl
  • autophagosome Source: UniProtKB
  • autophagosome membrane Source: UniProtKB
  • axoneme Source: UniProtKB
  • cell body Source: Ensembl
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: GO_Central
  • Golgi membrane Source: UniProtKB-SubCell
  • lysosome Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • microtubule associated complex Source: Ensembl
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: ProtInc
  • smooth endoplasmic reticulum Source: Ensembl
  • sperm midpiece Source: Ensembl

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49 – 50YL → AA: Inhibits interaction with TECPR2. 1 Publication2
Mutagenesisi67R → A: No effect on interaction with TECPR2. 1 Publication1
Mutagenesisi116G → A: Impairs localization at the autophagosomal membrane. 1 Publication1

Organism-specific databases

DisGeNETi11337.
OpenTargetsiENSG00000170296.
PharmGKBiPA28480.

Polymorphism and mutation databases

BioMutaiGABARAP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123631 – 116Gamma-aminobutyric acid receptor-associated proteinAdd BLAST116
PropeptideiPRO_0000423065117Removed in mature form1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi116Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116 – 117Cleavage; by ATG4B2

Keywords - PTMi

Lipoprotein

Proteomic databases

EPDiO95166.
PaxDbiO95166.
PeptideAtlasiO95166.
PRIDEiO95166.

PTM databases

iPTMnetiO95166.
PhosphoSitePlusiO95166.

Miscellaneous databases

PMAP-CutDBiO95166.

Expressioni

Tissue specificityi

Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.2 Publications

Gene expression databases

BgeeiENSG00000170296.
CleanExiHS_GABARAP.
ExpressionAtlasiO95166. baseline and differential.
GenevisibleiO95166. HS.

Organism-specific databases

HPAiHPA065883.

Interactioni

Subunit structurei

Interacts with GPHN and NSF (By similarity). Interacts with ATG3, ATG7, ATG13, RB1CC1, GABRG2, beta-tubulin and ULK1 (PubMed:11825910, PubMed:12507496, PubMed:11096062, PubMed:23043107, PubMed:11146101, PubMed:9892355, PubMed:11729197). Interacts with CALR (PubMed:19154346). Interacts with DDX47 (PubMed:15977068). Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22470510, PubMed:22421968). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1 (PubMed:17580304). Interacts with MAPK15 (PubMed:22948227). Interacts with TECPR2 and PCM1 (PubMed:20562859, PubMed:24089205). Interacts with TBC1D5 (PubMed:22354992). Interacts with TRIM5 (PubMed:25127057). Interacts with MEFV and TRIM21 (PubMed:26347139). Interacts with KIF21B (By similarity).By similarity20 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • microtubule binding Source: Ensembl
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116465. 64 interactors.
DIPiDIP-35050N.
ELMiO95166.
IntActiO95166. 475 interactors.
MINTiMINT-206256.
STRINGi9606.ENSP00000306866.

Structurei

Secondary structure

1117
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Helixi11 – 24Combined sources14
Beta strandi28 – 35Combined sources8
Beta strandi47 – 52Combined sources6
Helixi57 – 68Combined sources12
Beta strandi77 – 80Combined sources4
Beta strandi87 – 90Combined sources4
Helixi91 – 98Combined sources8
Beta strandi101 – 103Combined sources3
Beta strandi105 – 111Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNUX-ray1.75A1-117[»]
1KLVNMR-A1-117[»]
1KM7NMR-A1-117[»]
1KOTNMR-A1-117[»]
3D32X-ray1.30A/B1-117[»]
3DOWX-ray2.30A1-117[»]
3WIMX-ray2.60A1-117[»]
4XC2X-ray1.90A/B/C/D3-116[»]
5DPSX-ray2.00A/B/C2-117[»]
ProteinModelPortaliO95166.
SMRiO95166.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95166.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 22Interaction with beta-tubulinAdd BLAST22
Regioni36 – 117Interaction with GPHNBy similarityAdd BLAST82
Regioni36 – 68Interaction with GABRG2Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
KOiK08341.
OMAiRVPVICE.
OrthoDBiEOG091G10QX.
PhylomeDBiO95166.
TreeFamiTF314556.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiView protein in InterPro
IPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiView protein in Pfam
PF02991. Atg8. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL
60 70 80 90 100
VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE
110
EDFFLYIAYS DESVYGL
Length:117
Mass (Da):13,918
Last modified:May 1, 1999 - v1
Checksum:iBC0B84B8A51C1E32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161586 mRNA. Translation: AAD47641.1.
AB030711 mRNA. Translation: BAB21549.1.
AF044671 mRNA. Translation: AAD02337.1.
AF067171 mRNA. Translation: AAD32455.1.
AF183425 mRNA. Translation: AAG09694.1.
CCDSiCCDS11092.1.
RefSeqiNP_009209.1. NM_007278.1.
UniGeneiHs.647421.

Genome annotation databases

EnsembliENST00000302386; ENSP00000306866; ENSG00000170296.
GeneIDi11337.
KEGGihsa:11337.

Similar proteinsi

Entry informationi

Entry nameiGBRAP_HUMAN
AccessioniPrimary (citable) accession number: O95166
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 1999
Last modified: August 30, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families