Reviewed, UniProtKB/Swiss-Prot O95166 (GBRAP_HUMAN)

Names and origin

Protein names

Recommended name:
    Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
    GABA(A) receptor-associated protein
    MM46

Gene names

Name:	GABARAP
Synonyms:	FLC3B
ORF Names:	HT004

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton By similarity.
Subunit structure	Interacts with GPHN and NSF By similarity. Interacts with GABRG2, beta-tubulin and ULK1.
Subcellular location	Intracytoplasmic membraneBy similarity. Cytoplasm › cytoskeletonBy similarity. Golgi apparatus membraneBy similarity. Note= Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules By similarity.
Tissue specificity	Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Membrane Microtubule
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein targeting Ref.1 Traceable author statement. Source: ProtInc synaptic transmission Ref.1 Traceable author statement. Source: ProtInc
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell autophagic vacuole membrane Inferred from direct assay. Source: UniProtKB microtubule Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	GABA receptor binding Ref.1 Inferred from physical interaction. Source: UniProtKB beta-tubulin binding Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ULK1	O75385	1	EBI-712001,EBI-908831

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Gamma-aminobutyric acid receptor-associated protein

PRO_0000212363

Regions

Region

1 – 22

22

Interaction with beta-tubulin

Region

36 – 117

82

Interaction with GPHN By similarity

Region

36 – 68

33

Interaction with GABRG2 Potential

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

11 – 24

14

Beta strand

28 – 35

8

Beta strand

48 – 52

5

Helix

57 – 67

11

Beta strand

77 – 80

4

Helix

91 – 98

8

Beta strand

105 – 111

7

Sequences

	Sequence		Length	Mass (Da)
	O95166-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BC0B84B8A51C1E32	FASTA	117	13,918
	10 20 30 40 50 60 MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF 70 80 90 100 110 YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton." Wang H., Bedford F.K., Brandon N.J., Moss S.J., Olsen R.W. Nature 397:69-72(1999) [PubMed: 9892355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GABRG2 AND BETA-TUBULIN. Tissue: Brain.
[2]	"Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A. Brain Res. Mol. Brain Res. 85:1-12(2000) [PubMed: 11146101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ULK1. Tissue: Frontal cortex.
[3]	Iijima M., Mitsui Y. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	Ye M., Fu G., Wu J., Zhou J., Zhang Q., Shen Y., Kan L., He K., Gu B., Chen S., Mao M., Chen Z. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hypothalamus.
[6]	"The X-ray crystal structure and putative ligand-derived peptide binding properties of gamma-aminobutyric acid receptor type A receptor-associated protein." Knight D., Harris R., McAlister M.S.B., Phelan J.P., Geddes S., Moss S.J., Driscoll P.C., Keep N.H. J. Biol. Chem. 277:5556-5561(2002) [PubMed: 11729197] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), INTERACTION WITH GABRG2; TUBULIN ALPHA AND TUBULIN BETA.
[7]	"Solution structure of human GABA(A) receptor-associated protein GABARAP: implications for biolgoical funcrion and its regulation." Stangler T., Mayr L.M., Willbold D. J. Biol. Chem. 277:13363-13366(2002) [PubMed: 11875056] [Abstract] Cited for: STRUCTURE BY NMR.
[8]	"1H, 13C and '5N resonance assignments of GABARAP, GABAA receptor associated protein." Kouno T., Miura K., Kanematsu T., Shirakawa M., Hirata M., Kawano K. J. Biomol. NMR 22:97-98(2002) [PubMed: 11885988] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF161586 mRNA. Translation: AAD47641.1.
AB030711 mRNA. Translation: BAB21549.1.
AF044671 mRNA. Translation: AAD02337.1.
AF067171 mRNA. Translation: AAD32455.1.
AF183425 mRNA. Translation: AAG09694.1.

RefSeq

NP_009209.1.

UniGene

Hs.647421

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GNU	X-ray	1.75	A	1-117	[»]
1KLV	NMR	-	A	1-117	[»]
1KM7	NMR	-	A	1-117	[»]
1KOT	NMR	-	A	1-117	[»]
3D32	X-ray	1.30	A/B	1-117	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O95166.

PTM databases

PhosphoSite

O95166.

Genome annotation databases

Ensembl

ENSG00000170296. Homo sapiens. [Contig view]

GeneID

11337.

KEGG

hsa:11337.

Organism-specific databases

H-InvDB

HIX0013108.

HGNC

HGNC:4067. GABARAP.

MIM

605125. gene.

PharmGKB

PA28480.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

O95166.

HOVERGEN

O95166.

Gene expression databases

ArrayExpress

O95166.

CleanEx

HS_GABARAP.

GermOnline

ENSG00000170296. Homo sapiens.

Family and domain databases

InterPro

IPR004241. MAP1_LC3.
[Graphical view]

PANTHER

PTHR10969. MAP1_LC3. 1 hit.

Pfam

PF02991. MAP1_LC3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

O95166.

NextBio

43075.

SOURCE

Search...

Entry information

Entry name

GBRAP_HUMAN

Accession

Primary (citable) accession number: O95166

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)