O95166 (GBRAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor-associated protein

Alternative name(s):
GABA(A) receptor-associated protein
MM46

Gene names

Name:	GABARAP
Synonyms:	FLC3B
ORF Names:	HT004

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy By similarity. Ref.6
Subunit structure	Interacts with GPHN and NSF By similarity. Interacts with GABRG2, beta-tubulin and ULK1. Interacts with CALR. Interacts with DDX47. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 Ref.12 Ref.13 Ref.17
Subcellular location	Endomembrane system By similarity. Cytoplasm › cytoskeleton By similarity. Golgi apparatus membrane By similarity. Cytoplasmic vesicle › autophagosome. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules By similarity. Ref.7 Ref.8
Tissue specificity	Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Ref.1 Ref.2
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Apoptosis Autophagy Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Golgi apparatus Membrane Microtubule
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from direct assay Ref.6. Source: UniProtKB autophagy Inferred from electronic annotation. Source: UniProtKB-KW microtubule cytoskeleton organization Inferred from electronic annotation. Source: Compara protein targeting Traceable author statement Ref.1. Source: ProtInc synaptic transmission Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell actin cytoskeleton Inferred from electronic annotation. Source: Compara autophagic vacuole membrane Inferred from direct assay PubMed 15169837. Source: UniProtKB cell body Inferred from electronic annotation. Source: Compara cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW lysosome Inferred from electronic annotation. Source: Compara microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule associated complex Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement Ref.1. Source: ProtInc smooth endoplasmic reticulum Inferred from electronic annotation. Source: Compara
Molecular_function	beta-tubulin binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG2A	Q2TAZ0	2	EBI-712001,EBI-2514077
ATG3	Q9NT62	5	EBI-712001,EBI-988094
ATG4B	Q9Y4P1	8	EBI-712001,EBI-712014
ATG5	Q9H1Y0	2	EBI-712001,EBI-1047414
ATG7	O95352	8	EBI-712001,EBI-987834
DDX47	Q9H0S4	3	EBI-712001,EBI-2515241
DYX1C1	Q8WXU2	4	EBI-712001,EBI-2946907
FNBP1	Q96RU3	2	EBI-712001,EBI-1111248
FNIP1	Q8TF40	5	EBI-712001,EBI-2946919
FYCO1	Q9BQS8	2	EBI-712001,EBI-2869338
GBAS	O75323	5	EBI-712001,EBI-307133
HADHA	P40939	5	EBI-712001,EBI-356720
IPO5	O00410	6	EBI-712001,EBI-356424
KBTBD6	Q86V97	3	EBI-712001,EBI-2514778
KBTBD7	Q8WVZ9	2	EBI-712001,EBI-473695
NBR1	Q14596	5	EBI-712001,EBI-742698
NCOA7	Q8NI08	4	EBI-712001,EBI-80799
NEDD4	P46934	6	EBI-712001,EBI-726944
NEK9	Q8TD19	5	EBI-712001,EBI-1044009
NSMAF	Q92636	2	EBI-712001,EBI-2947053
RASSF1	Q9NS23	2	EBI-712001,EBI-367363
RASSF5	Q8WWW0	2	EBI-712001,EBI-367390
SQSTM1	Q13501	10	EBI-712001,EBI-307104
STBD1	O95210	5	EBI-712001,EBI-2947137
STK3	Q13188	2	EBI-712001,EBI-992580
STK4	Q13043	2	EBI-712001,EBI-367376
TBC1D15	Q8TC07	5	EBI-712001,EBI-1048247
TBC1D2B	Q9UPU7	2	EBI-712001,EBI-2947180
TECPR2	O15040	2	EBI-712001,EBI-2946991
UBA5	Q9GZZ9	2	EBI-712001,EBI-747805

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Gamma-aminobutyric acid receptor-associated protein

PRO_0000212363

Regions

Region

1 – 22

22

Interaction with beta-tubulin

Region

36 – 117

82

Interaction with GPHN By similarity

Region

36 – 68

33

Interaction with GABRG2 Potential

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

11 – 24

14

Beta strand

28 – 35

8

Beta strand

47 – 52

6

Helix

57 – 68

12

Beta strand

77 – 80

4

Beta strand

87 – 90

4

Helix

91 – 98

8

Beta strand

101 – 103

3

Beta strand

105 – 111

7

Sequences

	Sequence		Length	Mass (Da)
	O95166 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BC0B84B8A51C1E32	FASTA	117	13,918
	10 20 30 40 50 60 MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF 70 80 90 100 110 YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton." Wang H., Bedford F.K., Brandon N.J., Moss S.J., Olsen R.W. Nature 397:69-72(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GABRG2 AND BETA-TUBULIN. Tissue: Brain.
[2]	"Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A. Brain Res. Mol. Brain Res. 85:1-12(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ULK1. Tissue: Frontal cortex.
[3]	Iijima M., Mitsui Y. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	Ye M., Fu G., Wu J., Zhou J., Zhang Q., Shen Y., Kan L., He K., Gu B., Chen S., Mao M., Chen Z. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hypothalamus.
[6]	"GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)." Lee J.H., Rho S.B., Chun T. Biotechnol. Lett. 27:623-628(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH DDX47.
[7]	"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy." Pankiv S., Clausen T.H., Lamark T., Brech A., Bruun J.A., Outzen H., Overvatn A., Bjorkoy G., Johansen T. J. Biol. Chem. 282:24131-24145(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
[8]	"The TP53INP2 protein is required for autophagy in mammalian cells." Nowak J., Archange C., Tardivel-Lacombe J., Pontarotti P., Pebusque M.J., Vaccaro M.I., Velasco G., Dagorn J.C., Iovanna J.L. Mol. Biol. Cell 20:870-881(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TP53INP2.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation." Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M. J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TBC1D25.
[11]	"TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death." Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J. Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1.
[12]	"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription." Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A. PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1 AND TP53INP2.
[13]	"The X-ray crystal structure and putative ligand-derived peptide binding properties of gamma-aminobutyric acid receptor type A receptor-associated protein." Knight D., Harris R., McAlister M.S.B., Phelan J.P., Geddes S., Moss S.J., Driscoll P.C., Keep N.H. J. Biol. Chem. 277:5556-5561(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), INTERACTION WITH GABRG2; TUBULIN ALPHA AND TUBULIN BETA.
[14]	"Solution structure of human GABA(A) receptor-associated protein GABARAP: implications for biological function and its regulation." Stangler T., Mayr L.M., Willbold D. J. Biol. Chem. 277:13363-13366(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[15]	"1H, 13C and '5N resonance assignments of GABARAP, GABAA receptor associated protein." Kouno T., Miura K., Kanematsu T., Shirakawa M., Hirata M., Kawano K. J. Biomol. NMR 22:97-98(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[16]	"Ligand binding mode of GABAA receptor-associated protein." Weiergraber O.H., Stangler T., Thielmann Y., Mohrluder J., Wiesehan K., Willbold D. J. Mol. Biol. 381:1320-1331(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS).
[17]	"Structural framework of the GABARAP-calreticulin interface --implications for substrate binding to endoplasmic reticulum chaperones." Thielmann Y., Weiergraber O.H., Mohrluder J., Willbold D. FEBS J. 276:1140-1152(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH CALR, INTERACTION WITH CALR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF161586 mRNA. Translation: AAD47641.1.
AB030711 mRNA. Translation: BAB21549.1.
AF044671 mRNA. Translation: AAD02337.1.
AF067171 mRNA. Translation: AAD32455.1.
AF183425 mRNA. Translation: AAG09694.1.

IPI

IPI00027253.

RefSeq

NP_009209.1. NM_007278.1.

UniGene

Hs.647421.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GNU	X-ray	1.75	A	1-117	[»]
1KLV	NMR	-	A	1-117	[»]
1KM7	NMR	-	A	1-117	[»]
1KOT	NMR	-	A	1-117	[»]
3D32	X-ray	1.30	A/B	1-117	[»]
3DOW	X-ray	2.30	A	1-117	[»]

ProteinModelPortal

O95166.

ModBase

Search...

Protein-protein interaction databases

IntAct

O95166. 471 interactions.

MINT

MINT-206256.

STRING

9606.ENSP00000306866.

Protein family/group databases

TCDB

1.A.9.5.2. neurotransmitter receptor, cys loop, ligand-gated ion channel (LIC) family.

PTM databases

PhosphoSite

O95166.

Proteomic databases

PaxDb

O95166.

PRIDE

O95166.

Protocols and materials databases

DNASU

11337.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000302386; ENSP00000306866; ENSG00000170296.

GeneID

11337.

KEGG

hsa:11337.

UCSC

uc002gfb.3. human.

Organism-specific databases

CTD

11337.

GeneCards

GC17M007143.

HGNC

HGNC:4067. GABARAP.

MIM

605125. gene.

neXtProt

NX_O95166.

PharmGKB

PA28480.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG263746.

HOGENOM

HOG000232034.

HOVERGEN

HBG051706.

InParanoid

O95166.

KO

K08341.

OMA

TTMGQLY.

OrthoDB

EOG4T4CWW.

Gene expression databases

ArrayExpress

O95166.

Bgee

O95166.

CleanEx

HS_GABARAP.

Genevestigator

O95166.

GermOnline

ENSG00000170296. Homo sapiens.

Family and domain databases

InterPro

IPR004241. Atg8_ubiquitin-like.
[Graphical view]

PANTHER

PTHR10969. PTHR10969. 1 hit.

Pfam

PF02991. Atg8. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

GABARAP. human.

EvolutionaryTrace

O95166.

GenomeRNAi

11337.

NextBio

43075.

PMAP-CutDB

O95166.

SOURCE

Search...

Entry information

Entry name

GBRAP_HUMAN

Accession

Primary (citable) accession number: O95166

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.