O95164 (UBL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like protein 3

Alternative name(s):
Membrane-anchored ubiquitin-fold protein
Short name=HsMUB
Short name=MUB
Protein HCG-1

Gene names

Name:	UBL3
Synonyms:	PNSC1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor By similarity.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	intracellular Inferred from direct assay. Source: LIFEdb plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 114

114

Ubiquitin-like protein 3

PRO_0000114862

Propeptide

115 – 117

3

Removed in mature form Probable

PRO_0000248183

Regions

Domain

10 – 88

79

Ubiquitin-like

Amino acid modifications

Modified residue

114

1

Cysteine methyl ester Probable

Lipidation

114

1

S-geranylgeranyl cysteine Probable

Experimental info

Sequence conflict

10

1

I → V in CAG38489. Ref.3

Sequence conflict

28

1

P → H in AAH44582. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

117

Helix Strand Turn

Details...

Beta strand

10 – 15

6

Beta strand

21 – 26

6

Helix

32 – 40

9

Helix

55 – 57

3

Beta strand

58 – 66

9

Helix

74 – 76

3

Beta strand

82 – 90

9

Beta strand

95 – 99

5

Sequences

	Sequence		Length	Mass (Da)
	O95164 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 4EB1375D9C0B029F	FASTA	117	13,157
	10 20 30 40 50 60 MSSNVPADMI NLRLILVSGK TKEFLFSPND SASDIAKHVY DNWPMDWEEE QVSSPNILRL 70 80 90 100 110 IYQGRFLHGN VTLGALKLPF GKTTVMHLVA RETLPEPNSQ GQRNREKTGE SNCCVIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, mapping and expression of UBL3, a novel ubiquitin-like gene." Chadwick B.P., Kidd T., Sgouros J., Ish-Horowicz D., Frischauf A.-M. Gene 233:189-195(1999) [PubMed: 10375635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"MUBS: a family of ubiquitin-fold proteins that are plasma membrane-anchored by prenylation." Downes B.P., Saracco S.A., Lee S.S., Crowell D.N., Vierstra R.D. J. Biol. Chem. 281:27145-27157(2006) [PubMed: 16831869] [Abstract] Cited for: IDENTIFICATION, NOMENCLATURE.
[7]	"Solution structure of a membrane-anchored ubiquitin-fold (MUB) protein from Homo sapiens." de la Cruz N.B., Peterson F.C., Lytle B.L., Volkman B.F. Protein Sci. 16:1479-1484(2007) [PubMed: 17567738] [Abstract] Cited for: STRUCTURE BY NMR OF 2-117.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF044221 mRNA. Translation: AAD02323.1.
AL080177 mRNA. Translation: CAB45762.1.
CR533458 mRNA. Translation: CAG38489.1.
AL158193, AL139188 Genomic DNA. Translation: CAH73447.1.
AL139188, AL158193 Genomic DNA. Translation: CAI17023.1.
BC044582 mRNA. Translation: AAH44582.1.
BC059385 mRNA. Translation: AAH59385.1.

IPI

IPI00026669.

PIR

T12498.

RefSeq

NP_009037.1. NM_007106.3.

UniGene

Hs.145575.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GOW	NMR	-	A	2-117	[»]

ProteinModelPortal

O95164.

SMR

O95164. Positions 2-117.

ModBase

Search...

Protein-protein interaction databases

STRING

O95164.

PTM databases

PhosphoSite

O95164.

Proteomic databases

PRIDE

O95164.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000380680; ENSP00000370055; ENSG00000122042.

GeneID

5412.

KEGG

hsa:5412.

UCSC

uc001usp.1. human.

Organism-specific databases

CTD

5412.

GeneCards

GC13M030338.

H-InvDB

HIX0011206.

HGNC

HGNC:12504. UBL3.

MIM

604711. gene.

neXtProt

NX_O95164.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG20015.

GeneTree

ENSGT00390000004952.

HOGENOM

HBG382950.

HOVERGEN

HBG054980.

OrthoDB

EOG483D61.

PhylomeDB

O95164.

Gene expression databases

ArrayExpress

O95164.

Bgee

O95164.

CleanEx

HS_UBL3.

Genevestigator

O95164.

GermOnline

ENSG00000122042. Homo sapiens.

Family and domain databases

InterPro

IPR017000. M-anchored_Ub-fold_HCG-1.
IPR019955. Ubiquitin_supergroup.
[Graphical view]

PIRSF

PIRSF032572. MUB. 1 hit.

PROSITE

PS00299. UBIQUITIN_1. False negative.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

20953.

SOURCE

Search...

Entry information

Entry name

UBL3_HUMAN

Accession

Primary (citable) accession number: O95164
Secondary accession number(s): Q5RL72 , Q5VZS0, Q6FIG8, Q96SG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.