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Protein

Zinc finger protein-like 1

Gene

ZFPL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cis-Golgi integrity and efficient ER to Golgi transport. Involved in the maintenance of the integrity of the cis-Golgi, possibly via its interaction with GOLGA2/GM130.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 4343B box-type; degenerateAdd
BLAST
Zinc fingeri53 – 10149RING-type; degenerateAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein-like 1
Alternative name(s):
Zinc finger protein MCG4
Gene namesi
Name:ZFPL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:12868. ZFPL1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 266266CytoplasmicSequence analysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence analysisAdd
BLAST
Topological domaini288 – 31023LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: HPA
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271C → A: Impairs the interaction with OLGA2/GM130 and cis-Golgi assembly. 1 Publication
Mutagenesisi53 – 531C → A: Impairs the interaction with OLGA2/GM130 and cis-Golgi assembly. 1 Publication

Organism-specific databases

PharmGKBiPA37457.

Polymorphism and mutation databases

BioMutaiZFPL1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Zinc finger protein-like 1PRO_0000056318Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO95159.
MaxQBiO95159.
PaxDbiO95159.
PeptideAtlasiO95159.
PRIDEiO95159.

PTM databases

iPTMnetiO95159.
PhosphoSiteiO95159.

Expressioni

Tissue specificityi

Expressed strongly in the exocrine pancreas.1 Publication

Gene expression databases

BgeeiO95159.
CleanExiHS_ZFPL1.
ExpressionAtlasiO95159. baseline and differential.
GenevisibleiO95159. HS.

Organism-specific databases

HPAiHPA014909.
HPA017347.

Interactioni

Subunit structurei

Interacts with GOLGA2/GM130.1 Publication

Protein-protein interaction databases

BioGridi113374. 22 interactions.
IntActiO95159. 3 interactions.
MINTiMINT-1416552.
STRINGi9606.ENSP00000294258.

Structurei

3D structure databases

ProteinModelPortaliO95159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The B box-type and RING-type zinc fingers although degenerate play a central role in function of the protein.

Sequence similaritiesi

Belongs to the ZFPL1 family.Curated
Contains 1 B box-type zinc finger.Curated
Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 4343B box-type; degenerateAdd
BLAST
Zinc fingeri53 – 10149RING-type; degenerateAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG3970. Eukaryota.
ENOG410ZR2P. LUCA.
GeneTreeiENSGT00390000009753.
HOGENOMiHOG000031475.
HOVERGENiHBG079377.
InParanoidiO95159.
OMAiLIIIMAK.
PhylomeDBiO95159.
TreeFamiTF315090.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O95159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLCKCPKRK VTNLFCFEHR VNVCEHCLVA NHAKCIVQSY LQWLQDSDYN
60 70 80 90 100
PNCRLCNIPL ASRETTRLVC YDLFHWACLN ERAAQLPRNT APAGYQCPSC
110 120 130 140 150
NGPIFPPTNL AGPVASALRE KLATVNWARA GLGLPLIDEV VSPEPEPLNT
160 170 180 190 200
SDFSDWSSFN ASSTPGPEEV DSASAAPAFY SQAPRPPASP GRPEQHTVIH
210 220 230 240 250
MGNPEPLTHA PRKVYDTRDD DRTPGLHGDC DDDKYRRRPA LGWLARLLRS
260 270 280 290 300
RAGSRKRPLT LLQRAGLLLL LGLLGFLALL ALMSRLGRAA ADSDPNLDPL
310
MNPHIRVGPS
Length:310
Mass (Da):34,114
Last modified:July 5, 2004 - v2
Checksum:iA12F1CA68A6E7F4F
GO

Sequence cautioni

The sequence AAB86533.1 differs from that shown. Reason: Frameshift at positions 110, 114, 127, 156, 157 and 255. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821Q → R in AAB86533 (PubMed:9253601).Curated
Sequence conflicti182 – 1821Q → R in AAD08644 (Ref. 3) Curated
Sequence conflicti246 – 2461R → Q in AAB86533 (PubMed:9253601).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti218 – 2181R → Q.
Corresponds to variant rs35251366 [ dbSNP | Ensembl ].
VAR_034472

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001891 mRNA. Translation: AAB86533.1. Frameshift.
AF030291 mRNA. Translation: AAF21634.1.
AF043611 mRNA. Translation: AAD08644.1.
AK291964 mRNA. Translation: BAF84653.1.
AP003068 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74344.1.
BC012530 mRNA. Translation: AAH12530.1.
BC012814 mRNA. Translation: AAH12814.1.
CCDSiCCDS8092.1.
RefSeqiNP_006773.2. NM_006782.3.
UniGeneiHs.712317.
Hs.98170.

Genome annotation databases

EnsembliENST00000294258; ENSP00000294258; ENSG00000162300.
GeneIDi7542.
KEGGihsa:7542.
UCSCiuc001ocq.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001891 mRNA. Translation: AAB86533.1. Frameshift.
AF030291 mRNA. Translation: AAF21634.1.
AF043611 mRNA. Translation: AAD08644.1.
AK291964 mRNA. Translation: BAF84653.1.
AP003068 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74344.1.
BC012530 mRNA. Translation: AAH12530.1.
BC012814 mRNA. Translation: AAH12814.1.
CCDSiCCDS8092.1.
RefSeqiNP_006773.2. NM_006782.3.
UniGeneiHs.712317.
Hs.98170.

3D structure databases

ProteinModelPortaliO95159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113374. 22 interactions.
IntActiO95159. 3 interactions.
MINTiMINT-1416552.
STRINGi9606.ENSP00000294258.

PTM databases

iPTMnetiO95159.
PhosphoSiteiO95159.

Polymorphism and mutation databases

BioMutaiZFPL1.

Proteomic databases

EPDiO95159.
MaxQBiO95159.
PaxDbiO95159.
PeptideAtlasiO95159.
PRIDEiO95159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000294258; ENSP00000294258; ENSG00000162300.
GeneIDi7542.
KEGGihsa:7542.
UCSCiuc001ocq.2. human.

Organism-specific databases

CTDi7542.
GeneCardsiZFPL1.
HGNCiHGNC:12868. ZFPL1.
HPAiHPA014909.
HPA017347.
neXtProtiNX_O95159.
PharmGKBiPA37457.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3970. Eukaryota.
ENOG410ZR2P. LUCA.
GeneTreeiENSGT00390000009753.
HOGENOMiHOG000031475.
HOVERGENiHBG079377.
InParanoidiO95159.
OMAiLIIIMAK.
PhylomeDBiO95159.
TreeFamiTF315090.

Miscellaneous databases

ChiTaRSiZFPL1. human.
GenomeRNAii7542.
PROiO95159.

Gene expression databases

BgeeiO95159.
CleanExiHS_ZFPL1.
ExpressionAtlasiO95159. baseline and differential.
GenevisibleiO95159. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A transcript map for the 2.8-Mb region containing the multiple endocrine neoplasia type 1 locus."
    Guru S.C., Agarwal S.K., Manickam P., Olufemi S.-E., Crabtree J.S., Weisemann J.M., Kester M.B., Kim Y.S., Wang Y., Emmert-Buck M.R., Liotta L.A., Spiegel A.M., Boguski M.S., Roe B.A., Collins F.S., Burns A.L., Marx S.J., Chandrasekharappa S.C.
    Genome Res. 7:725-735(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  3. "Characterisation of a new human zinc-finger protein."
    Silins G.U., Grimmond S., Hayward N.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Neuroblastoma.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Muscle.
  8. "ZFPL1, a novel ring finger protein required for cis-Golgi integrity and efficient ER-to-Golgi transport."
    Chiu C.-F., Ghanekar Y., Frost L., Diao A., Morrison D., McKenzie E., Lowe M.
    EMBO J. 27:934-947(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, PHOSPHORYLATION, INTERACTION WITH GOLGA2, MUTAGENESIS OF CYS-27 AND CYS-53.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiZFPL1_HUMAN
AccessioniPrimary (citable) accession number: O95159
Secondary accession number(s): A8K7E9, O14616, Q9UID0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.