O95155 (UBE4B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin conjugation factor E4 B

Alternative name(s):
Homozygously deleted in neuroblastoma 1
Ubiquitin fusion degradation protein 2

Gene names

Name:	UBE4B
Synonyms:	HDNB1, KIAA0684, UFD2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 By similarity.
Subunit structure	Interacts with VCP By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen. Ref.3
Post-translational modification	Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7. Ref.3
Sequence similarities	Belongs to the ubiquitin conjugation factor E4 family. Contains 1 U-box domain.
Sequence caution	The sequence BAA31659.3 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from direct assay Ref.3. Source: UniProtKB protein ubiquitination involved in ubiquitin-dependent protein catabolic process Traceable author statement Ref.3. Source: UniProtKB response to UV Inferred from direct assay Ref.3. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin ligase complex Traceable author statement Ref.3. Source: UniProtKB
Molecular function	enzyme binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95155-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95155-2)

The sequence of this isoform differs from the canonical sequence as follows:
     270-398: Missing.

Isoform 3 (identifier: O95155-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     270-398: Missing.
     1234-1234: D → GKWTH

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1302

1302

Ubiquitin conjugation factor E4 B

PRO_0000194993

Regions

Domain

1231 – 1293

63

U-box

Sites

Site

109 – 110

2

Cleavage; by caspase-3 and caspase-7

Site

123 – 124

2

Cleavage; by caspase-6 and granzyme B

Amino acid modifications

Modified residue

22

1

Phosphothreonine Ref.13

Modified residue

26

1

Phosphothreonine Ref.13

Modified residue

31

1

Phosphoserine Ref.12 Ref.13

Modified residue

84

1

Phosphoserine Ref.15

Modified residue

87

1

Phosphoserine Ref.14

Modified residue

88

1

Phosphoserine Ref.13 Ref.14 Ref.15

Modified residue

90

1

Phosphoserine Ref.13 Ref.14 Ref.15

Modified residue

92

1

Phosphoserine Ref.14

Modified residue

95

1

Phosphothreonine Ref.14

Modified residue

101

1

Phosphoserine Ref.13 Ref.15

Modified residue

105

1

Phosphoserine Ref.9 Ref.14

Modified residue

803

1

Phosphoserine Ref.10 Ref.12 Ref.13

Modified residue

1265

1

Phosphoserine Ref.10 Ref.11

Natural variations

Alternative sequence

1 – 116

116

Missing in isoform 3.

VSP_007101

Alternative sequence

270 – 398

129

Missing in isoform 2 and isoform 3.

VSP_007102

Alternative sequence

1234

1

D → GKWTH in isoform 3.

VSP_007103

Natural variant

605

1

V → I.
Corresponds to variant rs17034499 [ dbSNP | Ensembl ].

VAR_052437

Experimental info

Mutagenesis

109

1

D → A: Abolishes cleavage by caspase-3 and caspase-7. Ref.3

Mutagenesis

121

1

D → A: Abolishes cleavage by caspase-6. No effect on cleavage by granzyme B. Ref.3

Mutagenesis

123

1

D → A: Abolishes cleavage by caspase-6 and granzyme B. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1302

Helix Strand Turn

Details...

Helix

1230 – 1232

3

Turn

1235 – 1237

3

Beta strand

1242 – 1246

5

Beta strand

1252 – 1254

3

Helix

1255 – 1264

10

Turn

1269 – 1271

3

Helix

1277 – 1279

3

Helix

1284 – 1298

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6BAA80984B03E43B	FASTA	1,302	146,185
	10 20 30 40 50 60 MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS QSLGLNVHNM 70 80 90 100 110 120 TPATSPIGAS GVAHRSQSSE GVSSLSSSPS NSLETQSQSL SRSQSMDIDG VSCEKSMSQV 130 140 150 160 170 180 DVDSGIENME VDENDRREKR SLSDKEPSSG PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL 190 200 210 220 230 240 SSLSAQFKQN PKEVFSDFKD LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIAAAARSPD 250 260 270 280 290 300 RNLLLNTGSN PGTSPMFCSV ASFGASSLSS LYESSPAPTP SFWSSVPVMG PSLASPSRAA 310 320 330 340 350 360 SQLAVPSTPL SPHSAASGTA AGSQPSSPRY RPYTVTHPWA SSGVSILSSS PSPPALASSP 370 380 390 400 410 420 QAVPASSSRQ RPSSTGPPLP PASPSATSRR PSSLRISPSL GASGGASNWD SYSDHFTIET 430 440 450 460 470 480 CKETDMLNYL IECFDRVGIE EKKAPKMCSQ PAVSQLLSNI RSQCISHTAL VLQGSLTQPR 490 500 510 520 530 540 SLQQPSFLVP YMLCRNLPYG FIQELVRTTH QDEEVFKQIF IPILQGLALA AKECSLDSDY 550 560 570 580 590 600 FKYPLMALGE LCETKFGKTH PVCNLVASLR LWLPKSLSPG CGRELQRLSY LGAFFSFSVF 610 620 630 640 650 660 AEDDVKVVEK YFSGPAITLE NTRVVSQSLQ HYLELGRQEL FKILHSILLN GETREAALSY 670 680 690 700 710 720 MAAVVNANMK KAQMQTDDRL VSTDGFMLNF LWVLQQLSTK IKLETVDPTY IFHPRCRITL 730 740 750 760 770 780 PNDETRVNAT MEDVNDWLTE LYGDQPPFSE PKFPTECFFL TLHAHHLSIL PSCRRYIRRL 790 800 810 820 830 840 RAIRELNRTV EDLKNNESQW KDSPLATRHR EMLKRCKTQL KKLVRCKACA DAGLLDESFL 850 860 870 880 890 900 RRCLNFYGLL IQLLLRILDP AYPDITLPLN SDVPKVFAAL PEFYVEDVAE FLFFIVQYSP 910 920 930 940 950 960 QALYEPCTQD IVMFLVVMLC NQNYIRNPYL VAKLVEVMFM TNPAVQPRTQ KFFEMIENHP 970 980 990 1000 1010 1020 LSTKLLVPSL MKFYTDVEHT GATSEFYDKF TIRYHISTIF KSLWQNIAHH GTFMEEFNSG 1030 1040 1050 1060 1070 1080 KQFVRYINML INDTTFLLDE SLESLKRIHE VQEEMKNKEQ WDQLPRDQQQ ARQSQLAQDE 1090 1100 1110 1120 1130 1140 RVSRSYLALA TETVDMFHIL TKQVQKPFLR PELGPRLAAM LNFNLQQLCG PKCRDLKVEN 1150 1160 1170 1180 1190 1200 PEKYGFEPKK LLDQLTDIYL QLDCARFAKA IADDQRSYSK ELFEEVISKM RKAGIKSTIA 1210 1220 1230 1240 1250 1260 IEKFKLLAEK VEEIVAKNAR AEIDYSDAPD EFRDPLMDTL MTDPVRLPSG TIMDRSIILR 1270 1280 1290 1300 HLLNSPTDPF NRQTLTESML EPVPELKEQI QAWMREKQNS DH
	Isoform 2 [UniParc]. Checksum: EBFE3FA2270B72B3	FASTA	1,173	133,275
	10 20 30 40 50 60 MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS QSLGLNVHNM 70 80 90 100 110 120 TPATSPIGAS GVAHRSQSSE GVSSLSSSPS NSLETQSQSL SRSQSMDIDG VSCEKSMSQV 130 140 150 160 170 180 DVDSGIENME VDENDRREKR SLSDKEPSSG PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL 190 200 210 220 230 240 SSLSAQFKQN PKEVFSDFKD LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIAAAARSPD 250 260 270 280 290 300 RNLLLNTGSN PGTSPMFCSV ASFGASSLSS LGASGGASNW DSYSDHFTIE TCKETDMLNY 310 320 330 340 350 360 LIECFDRVGI EEKKAPKMCS QPAVSQLLSN IRSQCISHTA LVLQGSLTQP RSLQQPSFLV 370 380 390 400 410 420 PYMLCRNLPY GFIQELVRTT HQDEEVFKQI FIPILQGLAL AAKECSLDSD YFKYPLMALG 430 440 450 460 470 480 ELCETKFGKT HPVCNLVASL RLWLPKSLSP GCGRELQRLS YLGAFFSFSV FAEDDVKVVE 490 500 510 520 530 540 KYFSGPAITL ENTRVVSQSL QHYLELGRQE LFKILHSILL NGETREAALS YMAAVVNANM 550 560 570 580 590 600 KKAQMQTDDR LVSTDGFMLN FLWVLQQLST KIKLETVDPT YIFHPRCRIT LPNDETRVNA 610 620 630 640 650 660 TMEDVNDWLT ELYGDQPPFS EPKFPTECFF LTLHAHHLSI LPSCRRYIRR LRAIRELNRT 670 680 690 700 710 720 VEDLKNNESQ WKDSPLATRH REMLKRCKTQ LKKLVRCKAC ADAGLLDESF LRRCLNFYGL 730 740 750 760 770 780 LIQLLLRILD PAYPDITLPL NSDVPKVFAA LPEFYVEDVA EFLFFIVQYS PQALYEPCTQ 790 800 810 820 830 840 DIVMFLVVML CNQNYIRNPY LVAKLVEVMF MTNPAVQPRT QKFFEMIENH PLSTKLLVPS 850 860 870 880 890 900 LMKFYTDVEH TGATSEFYDK FTIRYHISTI FKSLWQNIAH HGTFMEEFNS GKQFVRYINM 910 920 930 940 950 960 LINDTTFLLD ESLESLKRIH EVQEEMKNKE QWDQLPRDQQ QARQSQLAQD ERVSRSYLAL 970 980 990 1000 1010 1020 ATETVDMFHI LTKQVQKPFL RPELGPRLAA MLNFNLQQLC GPKCRDLKVE NPEKYGFEPK 1030 1040 1050 1060 1070 1080 KLLDQLTDIY LQLDCARFAK AIADDQRSYS KELFEEVISK MRKAGIKSTI AIEKFKLLAE 1090 1100 1110 1120 1130 1140 KVEEIVAKNA RAEIDYSDAP DEFRDPLMDT LMTDPVRLPS GTIMDRSIIL RHLLNSPTDP 1150 1160 1170 FNRQTLTESM LEPVPELKEQ IQAWMREKQN SDH
	Isoform 3 [UniParc]. Checksum: B4A29F16FFB1BD46	FASTA	1,061	121,820
	10 20 30 40 50 60 MSQVDVDSGI ENMEVDENDR REKRSLSDKE PSSGPEVSEE QALQLVCKIF RVSWKDRDRD 70 80 90 100 110 120 VIFLSSLSAQ FKQNPKEVFS DFKDLIGQIL MEVLMMSTQT RDENPFASLT ATSQPIAAAA 130 140 150 160 170 180 RSPDRNLLLN TGSNPGTSPM FCSVASFGAS SLSSLGASGG ASNWDSYSDH FTIETCKETD 190 200 210 220 230 240 MLNYLIECFD RVGIEEKKAP KMCSQPAVSQ LLSNIRSQCI SHTALVLQGS LTQPRSLQQP 250 260 270 280 290 300 SFLVPYMLCR NLPYGFIQEL VRTTHQDEEV FKQIFIPILQ GLALAAKECS LDSDYFKYPL 310 320 330 340 350 360 MALGELCETK FGKTHPVCNL VASLRLWLPK SLSPGCGREL QRLSYLGAFF SFSVFAEDDV 370 380 390 400 410 420 KVVEKYFSGP AITLENTRVV SQSLQHYLEL GRQELFKILH SILLNGETRE AALSYMAAVV 430 440 450 460 470 480 NANMKKAQMQ TDDRLVSTDG FMLNFLWVLQ QLSTKIKLET VDPTYIFHPR CRITLPNDET 490 500 510 520 530 540 RVNATMEDVN DWLTELYGDQ PPFSEPKFPT ECFFLTLHAH HLSILPSCRR YIRRLRAIRE 550 560 570 580 590 600 LNRTVEDLKN NESQWKDSPL ATRHREMLKR CKTQLKKLVR CKACADAGLL DESFLRRCLN 610 620 630 640 650 660 FYGLLIQLLL RILDPAYPDI TLPLNSDVPK VFAALPEFYV EDVAEFLFFI VQYSPQALYE 670 680 690 700 710 720 PCTQDIVMFL VVMLCNQNYI RNPYLVAKLV EVMFMTNPAV QPRTQKFFEM IENHPLSTKL 730 740 750 760 770 780 LVPSLMKFYT DVEHTGATSE FYDKFTIRYH ISTIFKSLWQ NIAHHGTFME EFNSGKQFVR 790 800 810 820 830 840 YINMLINDTT FLLDESLESL KRIHEVQEEM KNKEQWDQLP RDQQQARQSQ LAQDERVSRS 850 860 870 880 890 900 YLALATETVD MFHILTKQVQ KPFLRPELGP RLAAMLNFNL QQLCGPKCRD LKVENPEKYG 910 920 930 940 950 960 FEPKKLLDQL TDIYLQLDCA RFAKAIADDQ RSYSKELFEE VISKMRKAGI KSTIAIEKFK 970 980 990 1000 1010 1020 LLAEKVEEIV AKNARAEIDY SDAPDEFRGK WTHPLMDTLM TDPVRLPSGT IMDRSIILRH 1030 1040 1050 1060 LLNSPTDPFN RQTLTESMLE PVPELKEQIQ AWMREKQNSD H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression analysis of five novel genes in chromosome 1p36." Onyango P., Lubyova B., Gardellin P., Kurzbauer R., Weith A. Genomics 50:187-198(1998) [PubMed: 9653645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Identification and characterization of a 500-kb homozygously deleted region at 1p36.2-p36.3 in a neuroblastoma cell line." Ohira M., Kageyama H., Mihara M., Furuta S., Machida T., Shishikura T., Takayasu H., Islam A., Nakamura Y., Takahashi M., Tomioka N., Sakiyama S., Kaneko Y., Toyoda A., Hattori M., Sakaki Y., Ohki M., Horii A. , Soeda E., Inazawa J., Seki N., Kuma H., Nozawa I., Nakagawara A. Oncogene 19:4302-4307(2000) [PubMed: 10980605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Substantia nigra.
[3]	"The human homologue of the yeast polyubiquitination factor Ufd2p is cleaved by caspase 6 and granzyme B during apoptosis." Mahoney J.A., Odin J.A., White S.M., Shaffer D., Koff A., Casciola-Rosen L., Rosen A. Biochem. J. 361:587-595(2002) [PubMed: 11802788] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, MUTAGENESIS OF ASP-109; ASP-121 AND ASP-123, CLEAVAGE BY CASPASES.
[4]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[5]	Ohara O., Suyama M., Nagase T., Ishikawa K. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Cerebellum.
[8]	"Full-insert sequence of mapped XREF EST." Barrow I.K.-P., Boguski M.S., Touchman J.W., Spencer F. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1112-1302.
[9]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-803 AND SER-1265, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1265, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-803, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-22; THR-26; SER-31; SER-88; SER-90; SER-101 AND SER-803, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-88; SER-90; SER-92; THR-95 AND SER-105, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[15]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84; SER-88; SER-90 AND SER-101, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF043117 mRNA. Translation: AAD02233.1.
AB028839 mRNA. Translation: BAB40446.1.
AF331520 mRNA. Translation: AAK69622.1.
AB014584 mRNA. Translation: BAA31659.3. Different initiation.
AL590639, AL096841 Genomic DNA. Translation: CAI14687.1.
AL590639, AL096841 Genomic DNA. Translation: CAI14688.1.
AL096841, AL590639 Genomic DNA. Translation: CAI21859.1.
AL096841, AL590639 Genomic DNA. Translation: CAI21860.1.
BC093696 mRNA. Translation: AAH93696.1.
AF091093 mRNA. Translation: AAC72962.1. Sequence problems.

IPI

IPI00005715.
IPI00555735.
IPI00655534.

PIR

T00358.

RefSeq

NP_001099032.1. NM_001105562.2.
NP_006039.2. NM_006048.4.

UniGene

Hs.593974.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KRE	NMR	-	A	1208-1302	[»]
3L1X	X-ray	2.60	A	1208-1302	[»]
3L1Z	X-ray	3.17	B	1208-1302	[»]

ProteinModelPortal

O95155.

SMR

O95155. Positions 412-1300.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-40309N.

IntAct

O95155. 5 interactions.

MINT

MINT-2798575.

STRING

O95155.

PTM databases

PhosphoSite

O95155.

Proteomic databases

PRIDE

O95155.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000343090; ENSP00000343001; ENSG00000130939.

GeneID

10277.

KEGG

hsa:10277.

UCSC

uc001aqr.2. human.
uc001aqs.2. human.

Organism-specific databases

CTD

10277.

GeneCards

GC01P010092.

H-InvDB

HIX0023150.

HGNC

HGNC:12500. UBE4B.

HPA

HPA019219.

MIM

613565. gene.

neXtProt

NX_O95155.

PharmGKB

PA37148.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG09164.

GeneTree

ENSGT00390000009300.

HOVERGEN

HBG058129.

InParanoid

O95155.

OMA

FYGLLIQ.

PhylomeDB

O95155.

Gene expression databases

ArrayExpress

O95155.

Bgee

O95155.

CleanEx

HS_UBE4B.

Genevestigator

O95155.

GermOnline

ENSG00000130939. Homo sapiens.

Family and domain databases

InterPro

IPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

KO

K10597.

Pfam

PF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view]

SMART

SM00504. Ubox. 1 hit.
[Graphical view]

ProtoNet

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Other

NextBio

38932.

PMAP-CutDB

O95155.

SOURCE

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Entry information

Entry name

UBE4B_HUMAN

Accession

Primary (citable) accession number: O95155
Secondary accession number(s): O75169 , O95338, Q5SZ12, Q5SZ16, Q96QD4, Q9BYI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.