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Protein

Aflatoxin B1 aldehyde reductase member 3

Gene

AKR7A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44NADP1 Publication1
Active sitei49Proton donorBy similarity1
Sitei77Lowers pKa of active site TyrBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei169NADPBy similarity1
Binding sitei222NADP1 Publication1
Binding sitei232SubstrateBy similarity1
Binding sitei235SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi143 – 144NADP1 Publication2
Nucleotide bindingi198 – 208NADP1 PublicationAdd BLAST11
Nucleotide bindingi290 – 298NADP1 Publication9

GO - Molecular functioni

  • aldo-keto reductase (NADP) activity Source: Reactome
  • electron carrier activity Source: UniProtKB

GO - Biological processi

  • cellular aldehyde metabolic process Source: ProtInc
  • xenobiotic metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162482-MONOMER.
ReactomeiR-HSA-5423646. Aflatoxin activation and detoxification.
SABIO-RKO95154.

Names & Taxonomyi

Protein namesi
Recommended name:
Aflatoxin B1 aldehyde reductase member 3 (EC:1.-.-.-)
Alternative name(s):
AFB1 aldehyde reductase 2
Short name:
AFB1-AR 2
Gene namesi
Name:AKR7A3
Synonyms:AFAR2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:390. AKR7A3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000162482.
PharmGKBiPA24683.

Polymorphism and mutation databases

BioMutaiAKR7A3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000703781 – 331Aflatoxin B1 aldehyde reductase member 3Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1
Modified residuei85PhosphoserineCombined sources1
Modified residuei227PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO95154.
PaxDbiO95154.
PeptideAtlasiO95154.
PRIDEiO95154.

PTM databases

iPTMnetiO95154.
PhosphoSitePlusiO95154.

Expressioni

Tissue specificityi

Expressed in colon, kidney, liver, pancreas, adenocarcinoma and endometrium.2 Publications

Gene expression databases

BgeeiENSG00000162482.
CleanExiHS_AKR7A3.
GenevisibleiO95154. HS.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-748869,EBI-748869
AKR7A2O434883EBI-748869,EBI-748855
TERF2IPQ9NYB02EBI-748869,EBI-750109

Protein-protein interaction databases

BioGridi116626. 10 interactors.
IntActiO95154. 7 interactors.
MINTiMINT-1435043.
STRINGi9606.ENSP00000355377.

Structurei

Secondary structure

1331
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 15Combined sources5
Turni20 – 22Combined sources3
Helixi25 – 36Combined sources12
Turni37 – 39Combined sources3
Beta strandi42 – 44Combined sources3
Helixi49 – 52Combined sources4
Helixi53 – 59Combined sources7
Beta strandi67 – 69Combined sources3
Beta strandi73 – 78Combined sources6
Helixi88 – 102Combined sources15
Beta strandi107 – 112Combined sources6
Helixi121 – 133Combined sources13
Beta strandi136 – 144Combined sources9
Helixi147 – 160Combined sources14
Beta strandi165 – 171Combined sources7
Helixi178 – 180Combined sources3
Helixi183 – 190Combined sources8
Beta strandi193 – 197Combined sources5
Helixi201 – 206Combined sources6
Helixi211 – 214Combined sources4
Turni215 – 217Combined sources3
Beta strandi220 – 226Combined sources7
Helixi229 – 236Combined sources8
Helixi239 – 256Combined sources18
Helixi257 – 259Combined sources3
Helixi263 – 274Combined sources12
Helixi279 – 281Combined sources3
Beta strandi284 – 287Combined sources4
Helixi292 – 303Combined sources12
Helixi309 – 322Combined sources14
Helixi323 – 325Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CLPX-ray3.00A/B/C/D/E/F/G/H/I/J/K8-331[»]
ProteinModelPortaliO95154.
SMRiO95154.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95154.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IDYD. Eukaryota.
COG0667. LUCA.
GeneTreeiENSGT00550000074567.
HOGENOMiHOG000250286.
HOVERGENiHBG050576.
InParanoidiO95154.
KOiK15303.
OMAiAFVYSEG.
OrthoDBiEOG091G0BTK.
PhylomeDBiO95154.
TreeFamiTF329173.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
SUPFAMiSSF51430. SSF51430. 1 hit.

Sequencei

Sequence statusi: Complete.

O95154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFVYS
60 70 80 90 100
EGQSETILGG LGLRLGGSDC RVKIDTKAIP LFGNSLKPDS LRFQLETSLK
110 120 130 140 150
RLQCPRVDLF YLHMPDHSTP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV
160 170 180 190 200
AEICTLCKSN GWILPTVYQG MYNAITRQVE TELFPCLRHF GLRFYAFNPL
210 220 230 240 250
AGGLLTGKYK YEDKNGKQPV GRFFGNTWAE MYRNRYWKEH HFEGIALVEK
260 270 280 290 300
ALQAAYGASA PSMTSATLRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA
310 320 330
AAEEGPLEPA VVDAFNQAWH LVTHECPNYF R
Length:331
Mass (Da):37,206
Last modified:February 21, 2001 - v2
Checksum:iB9C32C33C7102AB3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51E → D in AAD02195 (PubMed:10383892).Curated1
Sequence conflicti247L → P in AAH42420 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017416138V → M.2 PublicationsCorresponds to variant rs2231198dbSNPEnsembl.1
Natural variantiVAR_017417215N → D.3 PublicationsCorresponds to variant rs1738023dbSNPEnsembl.1
Natural variantiVAR_017418323T → A.3 PublicationsCorresponds to variant rs1738025dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040639 mRNA. Translation: AAD02195.1.
AJ271799 mRNA. Translation: CAC81076.1.
AL035413 Genomic DNA. Translation: CAI22232.1.
BC025709 mRNA. Translation: AAH25709.1.
BC031562 mRNA. Translation: AAH31562.1.
BC042420 mRNA. Translation: AAH42420.2.
CCDSiCCDS193.1.
RefSeqiNP_036199.2. NM_012067.2.
UniGeneiHs.6980.

Genome annotation databases

EnsembliENST00000361640; ENSP00000355377; ENSG00000162482.
GeneIDi22977.
KEGGihsa:22977.
UCSCiuc001bbv.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040639 mRNA. Translation: AAD02195.1.
AJ271799 mRNA. Translation: CAC81076.1.
AL035413 Genomic DNA. Translation: CAI22232.1.
BC025709 mRNA. Translation: AAH25709.1.
BC031562 mRNA. Translation: AAH31562.1.
BC042420 mRNA. Translation: AAH42420.2.
CCDSiCCDS193.1.
RefSeqiNP_036199.2. NM_012067.2.
UniGeneiHs.6980.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CLPX-ray3.00A/B/C/D/E/F/G/H/I/J/K8-331[»]
ProteinModelPortaliO95154.
SMRiO95154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116626. 10 interactors.
IntActiO95154. 7 interactors.
MINTiMINT-1435043.
STRINGi9606.ENSP00000355377.

PTM databases

iPTMnetiO95154.
PhosphoSitePlusiO95154.

Polymorphism and mutation databases

BioMutaiAKR7A3.

Proteomic databases

MaxQBiO95154.
PaxDbiO95154.
PeptideAtlasiO95154.
PRIDEiO95154.

Protocols and materials databases

DNASUi22977.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361640; ENSP00000355377; ENSG00000162482.
GeneIDi22977.
KEGGihsa:22977.
UCSCiuc001bbv.2. human.

Organism-specific databases

CTDi22977.
GeneCardsiAKR7A3.
H-InvDBHIX0000196.
HGNCiHGNC:390. AKR7A3.
MIMi608477. gene.
neXtProtiNX_O95154.
OpenTargetsiENSG00000162482.
PharmGKBiPA24683.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IDYD. Eukaryota.
COG0667. LUCA.
GeneTreeiENSGT00550000074567.
HOGENOMiHOG000250286.
HOVERGENiHBG050576.
InParanoidiO95154.
KOiK15303.
OMAiAFVYSEG.
OrthoDBiEOG091G0BTK.
PhylomeDBiO95154.
TreeFamiTF329173.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162482-MONOMER.
ReactomeiR-HSA-5423646. Aflatoxin activation and detoxification.
SABIO-RKO95154.

Miscellaneous databases

EvolutionaryTraceiO95154.
GenomeRNAii22977.
PROiO95154.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162482.
CleanExiHS_AKR7A3.
GenevisibleiO95154. HS.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
SUPFAMiSSF51430. SSF51430. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARK73_HUMAN
AccessioniPrimary (citable) accession number: O95154
Secondary accession number(s): Q86SR4
, Q8IVN6, Q8N5V6, Q8TAX1, Q9NUC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 30, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.