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O95149 (SPN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Snurportin-1
Alternative name(s):
RNA U transporter 1
Gene names
Name:SNUPN
Synonyms:RNUT1, SPN1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs. Ref.1

Subunit structure

Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, Ran and XPO1. Interacts with DDX20, IPO7, KPNB1, SMN1, SNRPB and XPO1. Interacts directly with XPO1. Its interaction with XPO1 and binding to m3G-cap U snRNPs appears to be mutually exclusive. Ref.1 Ref.6 Ref.7

Subcellular location

Nucleus. Cytoplasm. Note: Nucleoplasmic shuttling protein. Its nuclear import involves the nucleocytoplasmic transport receptor importin beta. It is re-exported to the cytoplasm by the XPO1-dependent nuclear export receptor pathway. Ref.6 Ref.7

Sequence similarities

Belongs to the snurportin family.

Contains 1 IBB domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Snurportin-1
PRO_0000191071

Regions

Domain11 – 7363IBB
Region1 – 159159Necessary for interaction with XPO1
Region1 – 6565Necessary for interaction with KPNB1 and m3G-cap U1 and U5 snRNP import receptor activity
Region208 – 328121Necessary for binding to the m3G-cap structure

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10
Modified residue751Phosphoserine Ref.12

Experimental info

Mutagenesis271R → A: Abolishes interaction with KPNB1 and m3G-cap U1 snRNP import receptor activity. Ref.8
Mutagenesis1071W → A: Reduces binding to m3G-cap structure, interaction with XPO1 and snRNP import receptor activity. Ref.8 Ref.13
Mutagenesis203 – 2075FRFYW → A: Reduces binding to m3G-cap structure. Ref.8
Mutagenesis2761W → A: Reduces binding to m3G-cap structure, interaction with XPO1 and snRNP import receptor activity. Ref.8 Ref.13

Secondary structure

...................................................... 360
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95149 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 50B456D1C23B4BA1

FASTA36041,143
        10         20         30         40         50         60 
MEELSQALAS SFSVSQDLNS TAAPHPRLSQ YKSKYSSLEQ SERRRRLLEL QKSKRLDYVN 

        70         80         90        100        110        120 
HARRLAEDDW TGMESEEENK KDDEEMDIDT VKKLPKHYAN QLMLSEWLID VPSDLGQEWI 

       130        140        150        160        170        180 
VVVCPVGKRA LIVASRGSTS AYTKSGYCVN RFSSLLPGGN RRNSTAKDYT ILDCIYNEVN 

       190        200        210        220        230        240 
QTYYVLDVMC WRGHPFYDCQ TDFRFYWMHS KLPEEEGLGE KTKLNPFKFV GLKNFPCTPE 

       250        260        270        280        290        300 
SLCDVLSMDF PFEVDGLLFY HKQTHYSPGS TPLVGWLRPY MVSDVLGVAV PAGPLTTKPD 

       310        320        330        340        350        360 
YAGHQLQQIM EHKKSQKEGM KEKLTHKASE NGHYELEHLS TPKLKGSSHS PDHPGCLMEN 

« Hide

References

« Hide 'large scale' references
[1]"Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure."
Huber J., Cronshagen U., Kadokura M., Marshallsay C., Wada T., Sekine M., Luehrmann R.
EMBO J. 17:4114-4126(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 34-52; 54-69; 128-144; 211-221 AND 323-327, FUNCTION IN U SNRNP NUCLEAR IMPORT, INTERACTION WITH KPNB1, RNA-BINDING.
Tissue: Brain.
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cerebellum.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[6]"CRM1-mediated recycling of snurportin 1 to the cytoplasm."
Paraskeva E., Izaurralde E., Bischoff F.R., Huber J., Kutay U., Hartmann E., Luehrmann R., Goerlich D.
J. Cell Biol. 145:255-264(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A NUCLEAR EXPORT RECEPTOR COMPLEX, INTERACTION WITH IPO7; KPNB1 AND XPO1, IDENTIFICATION IN A TRIMERIC EXPORT COMPLEX WITH XPO1 AND RAN, SUBCELLULAR LOCATION.
[7]"SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta."
Narayanan U., Ospina J.K., Frey M.R., Hebert M.D., Matera A.G.
Hum. Mol. Genet. 11:1785-1795(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN AN IMPORT SNRNP COMPLEX, INTERACTION WITH DDX20; SMN1 AND SNRPB, SUBCELLULAR LOCATION.
[8]"Cross-talk between snurportin1 subdomains."
Ospina J.K., Gonsalvez G.B., Bednenko J., Darzynkiewicz E., Gerace L., Matera A.G.
Mol. Biol. Cell 16:4660-4671(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ARG-27; TRP-107; 203-PHE--TRP-207 AND TRP-276, RNA-BINDING.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1."
Strasser A., Dickmanns A., Luehrmann R., Ficner R.
EMBO J. 24:2235-2243(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 97-300 IN COMPLEX WITH M3G-CAP, MUTAGENESIS OF TRP-107 AND TRP-276.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039029 mRNA. Translation: AAC70906.1.
CR456811 mRNA. Translation: CAG33092.1.
AK289475 mRNA. Translation: BAF82164.1.
CH471136 Genomic DNA. Translation: EAW99245.1.
CH471136 Genomic DNA. Translation: EAW99246.1.
BC004203 mRNA. Translation: AAH04203.1.
RefSeqNP_001036046.1. NM_001042581.1.
NP_001036053.1. NM_001042588.1.
NP_005692.1. NM_005701.3.
UniGeneHs.21577.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1XK5X-ray2.40A97-300[»]
2P8QX-ray2.35B25-64[»]
2Q5DX-ray3.20C/D25-64[»]
2QNAX-ray2.84B1-66[»]
3GB8X-ray2.90B1-328[»]
3GJXX-ray2.50B/E1-360[»]
3LWWX-ray3.15B/D25-64[»]
3NBYX-ray3.42B/E15-360[»]
3NBZX-ray2.80B/E15-360[»]
3NC0X-ray2.90B/E15-360[»]
ProteinModelPortalO95149.
SMRO95149. Positions 12-360.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115384. 15 interactions.
DIPDIP-48513N.
IntActO95149. 11 interactions.
MINTMINT-1378592.
STRING9606.ENSP00000309831.

PTM databases

PhosphoSiteO95149.

Proteomic databases

PaxDbO95149.
PeptideAtlasO95149.
PRIDEO95149.

Protocols and materials databases

DNASU10073.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000308588; ENSP00000309831; ENSG00000169371.
ENST00000564644; ENSP00000454852; ENSG00000169371.
ENST00000564675; ENSP00000458053; ENSG00000169371.
ENST00000567134; ENSP00000456224; ENSG00000169371.
GeneID10073.
KEGGhsa:10073.
UCSCuc002ban.3. human.

Organism-specific databases

CTD10073.
GeneCardsGC15M075890.
HGNCHGNC:14245. SNUPN.
HPACAB005004.
MIM607902. gene.
neXtProtNX_O95149.
PharmGKBPA34611.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG317385.
HOGENOMHOG000012990.
HOVERGENHBG053257.
InParanoidO95149.
KOK13151.
OrthoDBEOG7J70GG.
PhylomeDBO95149.
TreeFamTF313108.

Enzyme and pathway databases

ReactomeREACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressO95149.
BgeeO95149.
CleanExHS_SNUPN.
GenevestigatorO95149.

Family and domain databases

InterProIPR002652. Importin-a_IBB.
IPR017336. Snurportin-1.
IPR024721. Snurportin-1_N.
[Graphical view]
PfamPF11538. Snurportin1. 1 hit.
[Graphical view]
PIRSFPIRSF037955. Snurportin-1. 1 hit.
PROSITEPS51214. IBB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO95149.
GeneWikiSNUPN.
GenomeRNAi10073.
NextBio38077.
PROO95149.
SOURCESearch...

Entry information

Entry nameSPN1_HUMAN
AccessionPrimary (citable) accession number: O95149
Secondary accession number(s): A6NE34, A8K0B0, D3DW76
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM