O95136 (S1PR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingosine 1-phosphate receptor 2
Short name=S1P receptor 2
Short name=S1P2

Alternative name(s):
Endothelial differentiation G-protein coupled receptor 5
Sphingosine 1-phosphate receptor Edg-5
Short name=S1P receptor Edg-5

Gene names

Name:	S1PR2
Synonyms:	EDG5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity Traceable author statement Ref.1. Source: ProtInc behavior Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc sphingosine-1-phosphate signaling pathway Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	G-protein coupled receptor activity Traceable author statement PubMed 8087418. Source: ProtInc lipid binding Traceable author statement Ref.1. Source: ProtInc sphingosine-1-phosphate receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 353

353

Sphingosine 1-phosphate receptor 2

PRO_0000069427

Regions

Topological domain

1 – 34

34

Extracellular By similarity

Transmembrane

35 – 59

25

Helical; Name=1; By similarity

Topological domain

60 – 66

7

Cytoplasmic By similarity

Transmembrane

67 – 95

29

Helical; Name=2; By similarity

Topological domain

96 – 109

14

Extracellular By similarity

Transmembrane

110 – 128

19

Helical; Name=3; By similarity

Topological domain

129 – 147

19

Cytoplasmic By similarity

Transmembrane

148 – 173

26

Helical; Name=4; By similarity

Topological domain

174 – 189

16

Extracellular By similarity

Transmembrane

190 – 210

21

Helical; Name=5; By similarity

Topological domain

211 – 233

23

Cytoplasmic By similarity

Transmembrane

234 – 255

22

Helical; Name=6; By similarity

Topological domain

256 – 271

16

Extracellular By similarity

Transmembrane

272 – 292

21

Helical; Name=7; By similarity

Topological domain

293 – 353

61

Cytoplasmic By similarity

Amino acid modifications

Modified residue

333

1

Phosphoserine By similarity

Lipidation

305

1

S-palmitoyl cysteine By similarity

Glycosylation

19

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

113

1

F → S in AAC98919. Ref.1

Sequence conflict

318

1

G → V in AAC98919. Ref.1

Secondary structure

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353

Helix Strand Turn

Details...

Beta strand

3 – 6

4

Beta strand

11 – 13

3

Turn

19 – 21

3

Turn

25 – 27

3

Helix

30 – 39

10

Beta strand

40 – 43

4

Helix

44 – 60

17

Helix

67 – 85

19

Turn

91 – 96

6

Helix

102 – 134

33

Beta strand

138 – 140

3

Helix

145 – 168

24

Beta strand

173 – 176

4

Turn

177 – 180

4

Beta strand

181 – 184

4

Helix

188 – 196

9

Helix

199 – 211

13

Beta strand

214 – 216

3

Helix

226 – 258

33

Helix

266 – 278

13

Turn

279 – 282

4

Helix

283 – 291

9

Helix

293 – 304

12

Turn

333 – 335

3

Beta strand

344 – 346

3

Sequences

	Sequence		Length	Mass (Da)
	O95136 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 8E37084284ABF7E8	FASTA	353	38,867
	10 20 30 40 50 60 MGSLYSEYLN PNKVQEHYNY TKETLETQET TSRQVASAFI VILCCAIVVE NLLVLIAVAR 70 80 90 100 110 120 NSKFHSAMYL FLGNLAASDL LAGVAFVANT LLSGSVTLRL TPVQWFAREG SAFITLSASV 130 140 150 160 170 180 FSLLAIAIER HVAIAKVKLY GSDKSCRMLL LIGASWLISL VLGGLPILGW NCLGHLEACS 190 200 210 220 230 240 TVLPLYAKHY VLCVVTIFSI ILLAIVALYV RIYCVVRSSH ADMAAPQTLA LLKTVTIVLG 250 260 270 280 290 300 VFIVCWLPAF SILLLDYACP VHSCPILYKA HYFFAVSTLN SLLNPVIYTW RSRDLRREVL 310 320 330 340 350 RPLQCWRPGV GVQGRRRGGT PGHHLLPLRS SSSLERGMHM PTSPTFLEGN TVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sphingosine 1-phosphate-induced cell proliferation, survival, and related signaling events mediated by G protein-coupled receptors Edg3 and Edg5." An S., Zheng Y., Bleu T. J. Biol. Chem. 275:288-296(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF034780 mRNA. Translation: AAC98919.1.
AY262688 Genomic DNA. Translation: AAP20652.1.
BC069598 mRNA. Translation: AAH69598.1.

IPI

IPI00003501.

RefSeq

NP_004221.3. NM_004230.3.

UniGene

Hs.731868.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZTI	model	-	A	1-353	[»]

ProteinModelPortal

O95136.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000322049.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

O95136.

Proteomic databases

PaxDb

O95136.

PRIDE

O95136.

Protocols and materials databases

DNASU

9294.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

9294.

KEGG

hsa:9294.

UCSC

uc002mnl.2. human.

Organism-specific databases

CTD

9294.

GeneCards

GC19M010332.

HGNC

HGNC:3169. S1PR2.

HPA

HPA014307.

MIM

605111. gene.

neXtProt

NX_O95136.

PharmGKB

PA162402353.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG149362.

HOGENOM

HOG000233501.

HOVERGEN

HBG103071.

InParanoid

O95136.

KO

K04292.

OMA

DKSCRML.

OrthoDB

EOG40ZQZC.

PhylomeDB

O95136.

Enzyme and pathway databases

Pathway_Interaction_DB

s1p_s1p2_pathway. S1P2 pathway.
s1p_s1p3_pathway. S1P3 pathway.
s1p_meta_pathway. Sphingosine 1-phosphate (S1P) pathway.

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

O95136.

CleanEx

HS_S1PR2.

Genevestigator

O95136.

GermOnline

ENSG00000175898. Homo sapiens.

Family and domain databases

InterPro

IPR004063. EDG5_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]

PANTHER

PTHR22750:SF17. PTHR22750:SF17. 1 hit.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR01525. EDG5RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

O95136.

ChEMBL

CHEMBL2955.

ChiTaRS

S1PR2. human.

GenomeRNAi

9294.

NextBio

34823.

SOURCE

Search...

Entry information

Entry name

S1PR2_HUMAN

Accession

Primary (citable) accession number: O95136
Secondary accession number(s): Q86UN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	November 7, 2003
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.