O95069 (KCNK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 2

Alternative name(s):
Outward rectifying potassium channel protein TREK-1
TREK-1 K(+) channel subunit
Two pore domain potassium channel TREK-1
Two pore potassium channel TPKC1

Gene names

Name:	KCNK2
Synonyms:	TREK, TREK1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Outward rectifying potassium channel.
Subunit structure	Homodimer Potential.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Activated by volatile general anesthetics such as chloroform, halothane and isoflurane.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	plasma membrane Traceable author statement. Source: Reactome voltage-gated potassium channel complex Inferred from electronic annotation. Source: Compara
Molecular_function	outward rectifier potassium channel activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95069-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95069-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-16: Missing.

Isoform 3 (identifier: O95069-3)

Also known as: c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MLPSASRERPGYRAGV → MMNPRAKRDFYL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Potassium channel subfamily K member 2		PRO_0000101742
Regions
	Topological domain	1 – 61	61	Cytoplasmic Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Intramembrane	144 – 170	27	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Topological domain	193 – 223	31	Cytoplasmic Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Intramembrane	253 – 283	31	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	288 – 308	21	Helical; Potential
	Topological domain	309 – 426	118	Cytoplasmic Potential
	Region	354 – 426	73	Required for basal channel activity By similarity
	Region	378 – 426	49	Essential for chloroform and halothane sensitivity By similarity
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 16	16	MLPSA…YRAGV → MMNPRAKRDFYL in isoform 3.		VSP_024428
	Alternative sequence	2 – 16	15	Missing in isoform 2.		VSP_024429
Experimental info
	Sequence conflict	309 – 311	3	DWL → RLV in AAD01203. Ref.3
	Sequence conflict	391	1	S → N in AAD47569. Ref.1
	Sequence conflict	411	1	T → A in AAD01203. Ref.3
	Sequence conflict	411	1	T → A in AAT49015. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: DB10382B1803DA13	FASTA	426	47,093
	10 20 30 40 50 60 MLPSASRERP GYRAGVAAPD LLDPKSAAQN SKPRLSFSTK PTVLASRVES DTTINVMKWK 70 80 90 100 110 120 TVSTIFLVVV LYLIIGATVF KALEQPHEIS QRTTIVIQKQ TFISQHSCVN STELDELIQQ 130 140 150 160 170 180 IVAAINAGII PLGNTSNQIS HWDLGSSFFF AGTVITTIGF GNISPRTEGG KIFCIIYALL 190 200 210 220 230 240 GIPLFGFLLA GVGDQLGTIF GKGIAKVEDT FIKWNVSQTK IRIISTIIFI LFGCVLFVAL 250 260 270 280 290 300 PAIIFKHIEG WSALDAIYFV VITLTTIGFG DYVAGGSDIE YLDFYKPVVW FWILVGLAYF 310 320 330 340 350 360 AAVLSMIGDW LRVISKKTKE EVGEFRAHAA EWTANVTAEF KETRRRLSVE IYDKFQRATS 370 380 390 400 410 420 IKRKLSAELA GNHNQELTPC RRTLSVNHLT SERDVLPPLL KTESIYLNGL TPHCAGEEIA VIENIK
	Isoform 2 [UniParc]. Checksum: FDE40CAB21B42A1C	FASTA	411	45,495
	10 20 30 40 50 60 MAAPDLLDPK SAAQNSKPRL SFSTKPTVLA SRVESDTTIN VMKWKTVSTI FLVVVLYLII 70 80 90 100 110 120 GATVFKALEQ PHEISQRTTI VIQKQTFISQ HSCVNSTELD ELIQQIVAAI NAGIIPLGNT 130 140 150 160 170 180 SNQISHWDLG SSFFFAGTVI TTIGFGNISP RTEGGKIFCI IYALLGIPLF GFLLAGVGDQ 190 200 210 220 230 240 LGTIFGKGIA KVEDTFIKWN VSQTKIRIIS TIIFILFGCV LFVALPAIIF KHIEGWSALD 250 260 270 280 290 300 AIYFVVITLT TIGFGDYVAG GSDIEYLDFY KPVVWFWILV GLAYFAAVLS MIGDWLRVIS 310 320 330 340 350 360 KKTKEEVGEF RAHAAEWTAN VTAEFKETRR RLSVEIYDKF QRATSIKRKL SAELAGNHNQ 370 380 390 400 410 ELTPCRRTLS VNHLTSERDV LPPLLKTESI YLNGLTPHCA GEEIAVIENI K
	Isoform 3 (c) [UniParc]. Checksum: 1AD4E0B5C1B6CBE7	FASTA	422	46,888
	10 20 30 40 50 60 MMNPRAKRDF YLAAPDLLDP KSAAQNSKPR LSFSTKPTVL ASRVESDTTI NVMKWKTVST 70 80 90 100 110 120 IFLVVVLYLI IGATVFKALE QPHEISQRTT IVIQKQTFIS QHSCVNSTEL DELIQQIVAA 130 140 150 160 170 180 INAGIIPLGN TSNQISHWDL GSSFFFAGTV ITTIGFGNIS PRTEGGKIFC IIYALLGIPL 190 200 210 220 230 240 FGFLLAGVGD QLGTIFGKGI AKVEDTFIKW NVSQTKIRII STIIFILFGC VLFVALPAII 250 260 270 280 290 300 FKHIEGWSAL DAIYFVVITL TTIGFGDYVA GGSDIEYLDF YKPVVWFWIL VGLAYFAAVL 310 320 330 340 350 360 SMIGDWLRVI SKKTKEEVGE FRAHAAEWTA NVTAEFKETR RRLSVEIYDK FQRATSIKRK 370 380 390 400 410 420 LSAELAGNHN QELTPCRRTL SVNHLTSERD VLPPLLKTES IYLNGLTPHC AGEEIAVIEN IK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inhalational anesthetics activate two-pore-domain background K+ channels." Patel A.J., Honore E., Lesage F., Fink M., Romey G., Lazdunski M. Nat. Neurosci. 2:422-426(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Cloning, localisation and functional expression of the human orthologue of the TREK-1 potassium channel." Meadows H.J., Benham C.D., Cairns W., Gloger I., Jennings C., Medhurst A.D., Murdock P., Chapman C.G. Pflugers Arch. 439:714-722(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[3]	Price L.A., Hellings S.E., Hayashi J.H., Pausch M.H. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Functional analysis of TREK-1 splice variants in rat and human." Preisig-Muller R., Daut J. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[5]	Thomas D., Sullivan A.N., Goldstein S.A. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain and Heart.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF129399 mRNA. Translation: AAD47569.1. AF171068 mRNA. Translation: AAF89743.1. AF004711 mRNA. Translation: AAD01203.1. AY552980 mRNA. Translation: AAT49015.1. EF165334 mRNA. Translation: ABM47413.1. EF165335 mRNA. Translation: ABM47414.1. AK291483 mRNA. Translation: BAF84172.1. AK315249 mRNA. Translation: BAG37671.1. CH471100 Genomic DNA. Translation: EAW93347.1. CH471100 Genomic DNA. Translation: EAW93349.1. BC069462 mRNA. Translation: AAH69462.1. BC101693 mRNA. Translation: AAI01694.1. BC101695 mRNA. Translation: AAI01696.1. BC143586 mRNA. Translation: AAI43587.1.
IPI	IPI00028268. IPI00829643. IPI00844492.
RefSeq	NP_001017424.1. NM_001017424.2. NP_001017425.2. NM_001017425.2. NP_055032.1. NM_014217.3.
UniGene	Hs.497745.
3D structure databases
ProteinModelPortal	O95069.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000394033.
PTM databases
PhosphoSite	O95069.
Proteomic databases
PaxDb	O95069.
PRIDE	O95069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000391894; ENSP00000375764; ENSG00000082482. ENST00000391895; ENSP00000375765; ENSG00000082482. ENST00000444842; ENSP00000394033; ENSG00000082482.
GeneID	3776.
KEGG	hsa:3776.
UCSC	uc001hko.3. human. uc001hkq.3. human. uc001hkr.4. human.
Organism-specific databases
CTD	3776.
GeneCards	GC01P215178.
HGNC	HGNC:6277. KCNK2.
HPA	HPA056054.
MIM	603219. gene.
neXtProt	NX_O95069.
PharmGKB	PA30059.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1226.
HOVERGEN	HBG052234.
InParanoid	O95069.
KO	K04913.
OMA	AINVMKW.
PhylomeDB	O95069.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	O95069.
Bgee	O95069.
CleanEx	HS_KCNK2.
Genevestigator	O95069.
GermOnline	ENSG00000082482. Homo sapiens.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR003976. 2pore_dom_K_chnl_TREK. IPR013099. Ion_trans_2. [Graphical view]
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PRINTS	PR01333. 2POREKCHANEL. PR01499. TREKCHANNEL.
ProtoNet	Search...
Other
ChiTaRS	KCNK2. human.
DrugBank	DB00204. Dofetilide.
GenomeRNAi	3776.
NextBio	14811.
SOURCE	Search...

Entry information

Entry name

KCNK2_HUMAN

Accession

Primary (citable) accession number: O95069
Secondary accession number(s): A1Z1V3 , A8K618, B2RCS4, B7ZL56, D3DTA5, Q5DP48, Q9NRT2, Q9UNE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	April 17, 2007
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.