Reviewed, UniProtKB/Swiss-Prot O95057 (DIRA1_HUMAN)

Names and origin

Protein names

Recommended name:
    GTP-binding protein Di-Ras1
Alternative name(s):
    Distinct subgroup of the Ras family member 1
    Ras-related inhibitor of cell growth
      Short name=Rig
    Small GTP-binding tumor suppressor 1

Gene names

Name:	DIRAS1
Synonyms:	GBTS1, RIG

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Displays low GTPase activity and exist predominantly in the GTP-bound form.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic sidePotential.
Tissue specificity	Highly expressed in heart and brain.
Sequence similarities	Belongs to the small GTPase superfamily. Di-Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Prenylation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

GTP-binding protein Di-Ras1

PRO_0000191648

Regions

Nucleotide binding

14 – 21

8

GTP By similarity

Nucleotide binding

61 – 65

5

GTP By similarity

Nucleotide binding

121 – 124

4

GTP By similarity

Motif

36 – 44

9

Effector region Potential

Amino acid modifications

Lipidation

195

1

S-geranylgeranyl cysteine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

198

Helix Strand Turn

Details...

Beta strand

8 – 14

7

Helix

20 – 29

10

Beta strand

42 – 50

9

Beta strand

53 – 61

9

Helix

64 – 66

3

Helix

69 – 78

10

Beta strand

80 – 87

8

Helix

91 – 95

5

Helix

98 – 108

11

Helix

111 – 113

3

Beta strand

116 – 121

6

Helix

132 – 142

11

Beta strand

145 – 148

4

Turn

151 – 154

4

Helix

157 – 167

11

Beta strand

169 – 171

3

Sequences

	Sequence		Length	Mass (Da)
	O95057-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 32E979AF80BB9A7F	FASTA	198	22,329
	10 20 30 40 50 60 MPEQSNDYRV VVFGAGGVGK SSLVLRFVKG TFRDTYIPTI EDTYRQVISC DKSVCTLQIT 70 80 90 100 110 120 DTTGSHQFPA MQRLSISKGH AFILVFSVTS KQSLEELGPI YKLIVQIKGS VEDIPVMLVG 130 140 150 160 170 180 NKCDETQREV DTREAQAVAQ EWKCAFMETS AKMNYNVKEL FQELLTLETR RNMSLNIDGK 190 RSGKQKRTDR VKGKCTLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Di-Ras, a distinct subgroup of ras family GTPases with unique biochemical properties." Kontani K., Tada M., Ogawa T., Okai T., Saito K., Araki Y., Katada T. J. Biol. Chem. 277:41070-41078(2002) [PubMed: 12194967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GTPASE ACTIVITY, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Rig is a novel Ras-related protein and potential neural tumor suppressor." Ellis C.A., Vos M.D., Howell H., Vallecorsa T., Fults D.W., Clark G.J. Proc. Natl. Acad. Sci. U.S.A. 99:9876-9881(2002) [PubMed: 12107278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[3]	"Molecular cloning of GBTS1, a novel gene encoding a small GTP-binding tumor suppressor." Gong L., Wu K. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Cismowski M.J., Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB076888 mRNA. Translation: BAC01115.1.
AY056037 mRNA. Translation: AAL23715.1.
AY059641 mRNA. Translation: AAL17968.1.
AY180973 mRNA. Translation: AAO22153.1.
AC006538 Genomic DNA. Translation: AAD13119.1.
BC030660 mRNA. Translation: AAH30660.1.

RefSeq

NP_660156.1.

UniGene

Hs.172753

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GF0	X-ray	1.90	A/B/C/D	1-198	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000176490. Homo sapiens. [Contig view]

GeneID

148252.

KEGG

hsa:148252.

Organism-specific databases

H-InvDB

HIX0014623.

HGNC

HGNC:19127. DIRAS1.

MIM

607862. gene.

PharmGKB

PA134951835.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

O95057.

HOVERGEN

O95057.

Gene expression databases

ArrayExpress

O95057.

CleanEx

HS_DIRAS1.

GermOnline

ENSG00000176490. Homo sapiens.

Family and domain databases

InterPro

IPR003577. GTPase_Ras.
IPR013753. Ras.
IPR001806. Ras_trnsfrmng.
IPR005225. Small_GTP_bd.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00173. RAS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

85883.

SOURCE

Search...

Entry information

Entry name

DIRA1_HUMAN

Accession

Primary (citable) accession number: O95057

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)