O95057 (DIRA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP-binding protein Di-Ras1

Alternative name(s):
Distinct subgroup of the Ras family member 1
Ras-related inhibitor of cell growth
Short name=Rig
Small GTP-binding tumor suppressor 1

Gene names

Name:	DIRAS1
Synonyms:	GBTS1, RIG

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays low GTPase activity and exist predominantly in the GTP-bound form.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Tissue specificity	Highly expressed in heart and brain. Ref.1 Ref.2
Sequence similarities	Belongs to the small GTPase superfamily. Di-Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 195

195

GTP-binding protein Di-Ras1

PRO_0000191648

Propeptide

196 – 198

3

Removed in mature form Potential

PRO_0000370775

Regions

Nucleotide binding

14 – 21

8

GTP By similarity

Nucleotide binding

61 – 65

5

GTP By similarity

Nucleotide binding

121 – 124

4

GTP By similarity

Motif

36 – 44

9

Effector region Potential

Amino acid modifications

Modified residue

195

1

Cysteine methyl ester Potential

Lipidation

195

1

S-geranylgeranyl cysteine By similarity

Secondary structure

1

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198

Helix Strand Turn

Details...

Beta strand

8 – 14

7

Helix

20 – 29

10

Beta strand

42 – 50

9

Beta strand

53 – 61

9

Helix

64 – 66

3

Helix

69 – 78

10

Beta strand

80 – 87

8

Helix

91 – 95

5

Helix

98 – 108

11

Helix

111 – 113

3

Beta strand

116 – 121

6

Helix

132 – 142

11

Beta strand

145 – 148

4

Turn

151 – 154

4

Helix

157 – 167

11

Beta strand

169 – 171

3

Sequences

	Sequence		Length	Mass (Da)
	O95057 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 32E979AF80BB9A7F	FASTA	198	22,329
	10 20 30 40 50 60 MPEQSNDYRV VVFGAGGVGK SSLVLRFVKG TFRDTYIPTI EDTYRQVISC DKSVCTLQIT 70 80 90 100 110 120 DTTGSHQFPA MQRLSISKGH AFILVFSVTS KQSLEELGPI YKLIVQIKGS VEDIPVMLVG 130 140 150 160 170 180 NKCDETQREV DTREAQAVAQ EWKCAFMETS AKMNYNVKEL FQELLTLETR RNMSLNIDGK 190 RSGKQKRTDR VKGKCTLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Di-Ras, a distinct subgroup of ras family GTPases with unique biochemical properties." Kontani K., Tada M., Ogawa T., Okai T., Saito K., Araki Y., Katada T. J. Biol. Chem. 277:41070-41078(2002) [PubMed: 12194967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GTPASE ACTIVITY, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Rig is a novel Ras-related protein and potential neural tumor suppressor." Ellis C.A., Vos M.D., Howell H., Vallecorsa T., Fults D.W., Clark G.J. Proc. Natl. Acad. Sci. U.S.A. 99:9876-9881(2002) [PubMed: 12107278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[3]	"Molecular cloning of GBTS1, a novel gene encoding a small GTP-binding tumor suppressor." Gong L., Wu K. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Cismowski M.J., Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB076888 mRNA. Translation: BAC01115.1.
AY056037 mRNA. Translation: AAL23715.1.
AY059641 mRNA. Translation: AAL17968.1.
AY180973 mRNA. Translation: AAO22153.1.
AC006538 Genomic DNA. Translation: AAD13119.1.
BC030660 mRNA. Translation: AAH30660.1.

IPI

IPI00002919.

RefSeq

NP_660156.1. NM_145173.3.

UniGene

Hs.172753.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GF0	X-ray	1.90	A/B/C/D	1-198	[»]

ProteinModelPortal

O95057.

SMR

O95057. Positions 3-176.

ModBase

Search...

Protein-protein interaction databases

STRING

O95057.

PTM databases

PhosphoSite

O95057.

Proteomic databases

PRIDE

O95057.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000323469; ENSP00000325836; ENSG00000176490.

GeneID

148252.

KEGG

hsa:148252.

UCSC

uc002lwf.2. human.

Organism-specific databases

CTD

148252.

GeneCards

GC19M002665.

H-InvDB

HIX0202801.

HGNC

HGNC:19127. DIRAS1.

MIM

607862. gene.

neXtProt

NX_O95057.

PharmGKB

PA134951835.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG13492.

GeneTree

ENSGT00560000076632.

HOGENOM

HBG745225.

HOVERGEN

HBG009351.

InParanoid

O95057.

OMA

LGPIYQL.

OrthoDB

EOG46MBKP.

PhylomeDB

O95057.

Gene expression databases

ArrayExpress

O95057.

Bgee

O95057.

CleanEx

HS_DIRAS1.

Genevestigator

O95057.

GermOnline

ENSG00000176490. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]

KO

K07840.

PANTHER

PTHR24070. PTHR24070. 1 hit.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00173. RAS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51421. RAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

85883.

SOURCE

Search...

Entry information

Entry name

DIRA1_HUMAN

Accession

Primary (citable) accession number: O95057

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.