Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Indolethylamine N-methyltransferase

Gene

INMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds (By similarity). Catalyzes the N-methylation of tryptamine and structurally related compounds.By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine.1 Publication
S-adenosyl-L-methionine + dimethyl sulfide = S-adenosyl-L-homocysteine + trimethylsulfonium.1 Publication

Kineticsi

  1. KM=2.9 mM for tryptamine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei20S-adenosyl-L-methionine1
    Binding sitei25S-adenosyl-L-methionine1
    Binding sitei63S-adenosyl-L-methionine; via carbonyl oxygen1
    Binding sitei69S-adenosyl-L-methionine1
    Binding sitei90S-adenosyl-L-methionine1
    Binding sitei163S-adenosyl-L-methionine; via carbonyl oxygen1

    GO - Molecular functioni

    • amine N-methyltransferase activity Source: UniProtKB
    • thioether S-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    • amine metabolic process Source: UniProtKB
    • methylation Source: UniProtKB
    • response to toxic substance Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Detoxification

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:HS00305-MONOMER.
    ZFISH:HS00305-MONOMER.
    ReactomeiR-HSA-2408552. Methylation of MeSeH for excretion.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Indolethylamine N-methyltransferase (EC:2.1.1.49, EC:2.1.1.96)
    Short name:
    Indolamine N-methyltransferase
    Alternative name(s):
    Aromatic alkylamine N-methyltransferase
    Short name:
    Amine N-methyltransferase
    Short name:
    Arylamine N-methyltransferase
    Thioether S-methyltransferase
    Short name:
    TEMT
    Gene namesi
    Name:INMT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:6069. INMT.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    OpenTargetsiENSG00000241644.
    PharmGKBiPA403.

    Chemistry databases

    ChEMBLiCHEMBL2131.

    Polymorphism and mutation databases

    BioMutaiINMT.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001597121 – 263Indolethylamine N-methyltransferaseAdd BLAST263

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei13N6-succinyllysineBy similarity1
    Modified residuei96N6-succinyllysineBy similarity1

    Proteomic databases

    PaxDbiO95050.
    PeptideAtlasiO95050.
    PRIDEiO95050.

    PTM databases

    iPTMnetiO95050.
    PhosphoSitePlusiO95050.

    Expressioni

    Tissue specificityi

    Widely expressed. The highest levels were in thyroid, adrenal gland, adult and fetal lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node and trachea. Very low levels in adult and fetal kidney and liver, in adult spleen, thymus, ovary, colon and bone marrow. Not expressed in peripheral blood leukocytes and brain.1 Publication

    Gene expression databases

    BgeeiENSG00000241644.
    CleanExiHS_INMT.
    GenevisibleiO95050. HS.

    Organism-specific databases

    HPAiHPA055180.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KLHL12Q53G595EBI-10191038,EBI-740929

    Protein-protein interaction databases

    BioGridi116355. 1 interactor.
    IntActiO95050. 2 interactors.

    Chemistry databases

    BindingDBiO95050.

    Structurei

    Secondary structure

    1263
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi8 – 14Combined sources7
    Helixi17 – 24Combined sources8
    Helixi33 – 49Combined sources17
    Beta strandi56 – 63Combined sources8
    Helixi69 – 71Combined sources3
    Helixi74 – 76Combined sources3
    Beta strandi78 – 86Combined sources9
    Helixi88 – 99Combined sources12
    Helixi108 – 117Combined sources10
    Helixi121 – 123Combined sources3
    Helixi124 – 134Combined sources11
    Beta strandi135 – 140Combined sources6
    Beta strandi145 – 147Combined sources3
    Turni148 – 151Combined sources4
    Beta strandi157 – 164Combined sources8
    Helixi166 – 169Combined sources4
    Helixi173 – 184Combined sources12
    Beta strandi187 – 200Combined sources14
    Beta strandi203 – 206Combined sources4
    Beta strandi209 – 212Combined sources4
    Helixi218 – 227Combined sources10
    Beta strandi230 – 238Combined sources9
    Turni244 – 246Combined sources3
    Beta strandi252 – 259Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2A14X-ray1.70A1-263[»]
    ProteinModelPortaliO95050.
    SMRiO95050.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95050.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni85 – 87S-adenosyl-L-methionine binding3
    Regioni142 – 143S-adenosyl-L-methionine binding2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410IFTZ. Eukaryota.
    ENOG41128ZR. LUCA.
    GeneTreeiENSGT00390000011708.
    HOGENOMiHOG000013229.
    HOVERGENiHBG000797.
    InParanoidiO95050.
    KOiK00562.
    OMAiAMECACC.
    OrthoDBiEOG091G0EF7.
    PhylomeDBiO95050.
    TreeFamiTF313114.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025820. NNMT/PNMT/TEMT_CS.
    IPR000940. NNMT_TEMT_trans.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PANTHERiPTHR10867. PTHR10867. 1 hit.
    PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000384. PNMTase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
    PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: O95050-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKGGFTGGDE YQKHFLPRDY LATYYSFDGS PSPEAEMLKF NLECLHKTFG
    60 70 80 90 100
    PGGLQGDTLI DIGSGPTIYQ VLAACDSFQD ITLSDFTDRN REELEKWLKK
    110 120 130 140 150
    EPGAYDWTPA VKFACELEGN SGRWEEKEEK LRAAVKRVLK CDVHLGNPLA
    160 170 180 190 200
    PAVLPLADCV LTLLAMECAC CSLDAYRAAL CNLASLLKPG GHLVTTVTLR
    210 220 230 240 250
    LPSYMVGKRE FSCVALEKEE VEQAVLDAGF DIEQLLHSPQ SYSVTNAANN
    260
    GVCFIVARKK PGP
    Length:263
    Mass (Da):28,891
    Last modified:November 13, 2007 - v3
    Checksum:i12B3AC66597E70A3
    GO
    Isoform 2 (identifier: O95050-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         52-52: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:262
    Mass (Da):28,834
    Checksum:i0DEF8C52DEFE814B
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti75C → F in AK313832 (PubMed:14702039).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_03699128D → N.1 PublicationCorresponds to variant rs4723010dbSNPEnsembl.1
    Natural variantiVAR_011616205M → V.1 PublicationCorresponds to variant rs2302339dbSNPEnsembl.1
    Natural variantiVAR_061373214V → M.Corresponds to variant rs56800285dbSNPEnsembl.1
    Natural variantiVAR_011617219E → G.3 PublicationsCorresponds to variant rs2302340dbSNPEnsembl.1
    Natural variantiVAR_036992246N → S.Corresponds to variant rs6970210dbSNPEnsembl.1
    Natural variantiVAR_036993254F → C.1 PublicationCorresponds to variant rs4720015dbSNPEnsembl.1
    Natural variantiVAR_036994258R → H.Corresponds to variant rs6970605dbSNPEnsembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_04592252Missing in isoform 2. 1 Publication1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF128846 mRNA. Translation: AAF18304.1.
    AF128847 mRNA. Translation: AAF18305.1.
    AF128848 Genomic DNA. Translation: AAF18306.1.
    AK313832 mRNA. No translation available.
    AC004976 Genomic DNA. No translation available.
    AC006022 Genomic DNA. Translation: AAD04723.1.
    BC033813 mRNA. Translation: AAH33813.1.
    BC106902 mRNA. Translation: AAI06903.1.
    BC106903 mRNA. Translation: AAI06904.1.
    AB041362 Genomic DNA. Translation: BAA94451.1.
    CCDSiCCDS5430.1. [O95050-1]
    CCDS56479.1. [O95050-2]
    RefSeqiNP_001186148.1. NM_001199219.1. [O95050-2]
    NP_006765.4. NM_006774.4. [O95050-1]
    UniGeneiHs.632629.

    Genome annotation databases

    EnsembliENST00000013222; ENSP00000013222; ENSG00000241644. [O95050-1]
    ENST00000409539; ENSP00000386961; ENSG00000241644. [O95050-2]
    GeneIDi11185.
    KEGGihsa:11185.
    UCSCiuc003tbs.1. human. [O95050-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF128846 mRNA. Translation: AAF18304.1.
    AF128847 mRNA. Translation: AAF18305.1.
    AF128848 Genomic DNA. Translation: AAF18306.1.
    AK313832 mRNA. No translation available.
    AC004976 Genomic DNA. No translation available.
    AC006022 Genomic DNA. Translation: AAD04723.1.
    BC033813 mRNA. Translation: AAH33813.1.
    BC106902 mRNA. Translation: AAI06903.1.
    BC106903 mRNA. Translation: AAI06904.1.
    AB041362 Genomic DNA. Translation: BAA94451.1.
    CCDSiCCDS5430.1. [O95050-1]
    CCDS56479.1. [O95050-2]
    RefSeqiNP_001186148.1. NM_001199219.1. [O95050-2]
    NP_006765.4. NM_006774.4. [O95050-1]
    UniGeneiHs.632629.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2A14X-ray1.70A1-263[»]
    ProteinModelPortaliO95050.
    SMRiO95050.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi116355. 1 interactor.
    IntActiO95050. 2 interactors.

    Chemistry databases

    BindingDBiO95050.
    ChEMBLiCHEMBL2131.

    PTM databases

    iPTMnetiO95050.
    PhosphoSitePlusiO95050.

    Polymorphism and mutation databases

    BioMutaiINMT.

    Proteomic databases

    PaxDbiO95050.
    PeptideAtlasiO95050.
    PRIDEiO95050.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000013222; ENSP00000013222; ENSG00000241644. [O95050-1]
    ENST00000409539; ENSP00000386961; ENSG00000241644. [O95050-2]
    GeneIDi11185.
    KEGGihsa:11185.
    UCSCiuc003tbs.1. human. [O95050-1]

    Organism-specific databases

    CTDi11185.
    GeneCardsiINMT.
    HGNCiHGNC:6069. INMT.
    HPAiHPA055180.
    MIMi604854. gene.
    neXtProtiNX_O95050.
    OpenTargetsiENSG00000241644.
    PharmGKBiPA403.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiENOG410IFTZ. Eukaryota.
    ENOG41128ZR. LUCA.
    GeneTreeiENSGT00390000011708.
    HOGENOMiHOG000013229.
    HOVERGENiHBG000797.
    InParanoidiO95050.
    KOiK00562.
    OMAiAMECACC.
    OrthoDBiEOG091G0EF7.
    PhylomeDBiO95050.
    TreeFamiTF313114.

    Enzyme and pathway databases

    BioCyciMetaCyc:HS00305-MONOMER.
    ZFISH:HS00305-MONOMER.
    ReactomeiR-HSA-2408552. Methylation of MeSeH for excretion.

    Miscellaneous databases

    EvolutionaryTraceiO95050.
    GenomeRNAii11185.
    PROiO95050.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000241644.
    CleanExiHS_INMT.
    GenevisibleiO95050. HS.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025820. NNMT/PNMT/TEMT_CS.
    IPR000940. NNMT_TEMT_trans.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PANTHERiPTHR10867. PTHR10867. 1 hit.
    PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000384. PNMTase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
    PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiINMT_HUMAN
    AccessioniPrimary (citable) accession number: O95050
    Secondary accession number(s): B8ZZ69
    , Q3KP49, Q9P1Y2, Q9UBY4, Q9UHQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 13, 2007
    Last modified: November 30, 2016
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.