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O95049 (ZO3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tight junction protein ZO-3
Alternative name(s):
Tight junction protein 3
Zona occludens protein 3
Zonula occludens protein 3
Gene names
Name:TJP3
Synonyms:ZO3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length919 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with occludin, claudins and ZO-1. Interacts with INADL By similarity. Interacts with UBN1. Interacts with FASLG. Ref.6 Ref.7

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell junctiontight junction By similarity.

Sequence similarities

Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

Sequence caution

The sequence AAC72274.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAW69293.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 5 (identifier: O95049-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 3 (identifier: O95049-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNLCGLMPIFPAPLDQVADM
Note: No experimental confirmation available.
Isoform 4 (identifier: O95049-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAVRFQVADM
Isoform 6 (identifier: O95049-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 919919Tight junction protein ZO-3
PRO_0000094546

Regions

Domain11 – 9383PDZ 1
Domain195 – 27278PDZ 2
Domain380 – 44667PDZ 3
Domain475 – 54975SH3
Domain580 – 761182Guanylate kinase-like

Amino acid modifications

Modified residue1641Phosphoserine Ref.5 Ref.8 Ref.9 Ref.10
Modified residue1691Phosphoserine By similarity
Modified residue9051Phosphoserine Ref.5 Ref.9
Modified residue9061Phosphoserine Ref.5 Ref.9

Natural variations

Alternative sequence1 – 3636Missing in isoform 6.
VSP_053844
Alternative sequence11M → MNLCGLMPIFPAPLDQVADM in isoform 3.
VSP_040238
Alternative sequence11M → MAVRFQVADM in isoform 4.
VSP_047022
Natural variant8981M → T. Ref.4
Corresponds to variant rs1046268 [ dbSNP | Ensembl ].
VAR_056114

Experimental info

Sequence conflict191R → H in AAI08907. Ref.4
Sequence conflict961H → L in BAG52285. Ref.1
Sequence conflict4491L → P in BAG54652. Ref.1
Sequence conflict8021R → H in BAG52285. Ref.1
Sequence conflict9011H → R in BAG52285. Ref.1
Sequence conflict9171T → M in BAG65278. Ref.1

Secondary structure

............................................. 919
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 5 [UniParc].

Last modified March 19, 2014. Version 3.
Checksum: D36EB684E7E4A6C8

FASTA919101,397
        10         20         30         40         50         60 
MEELTIWEQH TATLSKDPRR GFGIAISGGR DRPGGSMVVS DVVPGGPAEG RLQTGDHIVM 

        70         80         90        100        110        120 
VNGVSMENAT SAFAIQILKT CTKMANITVK RPRRIHLPAT KASPSSPGRQ DSDEDDGPQR 

       130        140        150        160        170        180 
VEEVDQGRGY DGDSSSGSGR SWDERSRRPR PGRRGRAGSH GRRSPGGGSE ANGLALVSGF 

       190        200        210        220        230        240 
KRLPRQDVQM KPVKSVLVKR RDSEEFGVKL GSQIFIKHIT DSGLAARHRG LQEGDLILQI 

       250        260        270        280        290        300 
NGVSSQNLSL NDTRRLIEKS EGKLSLLVLR DRGQFLVNIP PAVSDSDSSP LEDISDLASE 

       310        320        330        340        350        360 
LSQAPPSHIP PPPRHAQRSP EASQTDSPVE SPRLRRESSV DSRTISEPDE QRSELPRESS 

       370        380        390        400        410        420 
YDIYRVPSSQ SMEDRGYSPD TRVVRFLKGK SIGLRLAGGN DVGIFVSGVQ AGSPADGQGI 

       430        440        450        460        470        480 
QEGDQILQVN DVPFQNLTRE EAVQFLLGLP PGEEMELVTQ RKQDIFWKMV QSRVGDSFYI 

       490        500        510        520        530        540 
RTHFELEPSP PSGLGFTRGD VFHVLDTLHP GPGQSHARGG HWLAVRMGRD LREQERGIIP 

       550        560        570        580        590        600 
NQSRAEQLAS LEAAQRAVGV GPGSSAGSNA RAEFWRLRGL RRGAKKTTQR SREDLSALTR 

       610        620        630        640        650        660 
QGRYPPYERV VLREASFKRP VVILGPVADI AMQKLTAEMP DQFEIAETVS RTDSPSKIIK 

       670        680        690        700        710        720 
LDTVRVIAEK DKHALLDVTP SAIERLNYVQ YYPIVVFFIP ESRPALKALR QWLAPASRRS 

       730        740        750        760        770        780 
TRRLYAQAQK LRKHSSHLFT ATIPLNGTSD TWYQELKAII REQQTRPIWT AEDQLDGSLE 

       790        800        810        820        830        840 
DNLDLPHHGL ADSSADLSCD SRVNSDYETD GEGGAYTDGE GYTDGEGGPY TDVDDEPPAP 

       850        860        870        880        890        900 
ALARSSEPVQ ADESQSPRDR GRISAHQGAQ VDSRHPQGQW RQDSMRTYER EALKKKFMRV 

       910 
HDAESSDEDG YDWGPATDL 

« Hide

Isoform 3 [UniParc].

Checksum: 603411581EB7FA85
Show »

FASTA938103,424
Isoform 4 [UniParc].

Checksum: B418537346F205CF
Show »

FASTA928102,415
Isoform 6 [UniParc].

Checksum: 511564BFC624B3A8
Show »

FASTA88397,488

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 4; 5 AND 6).
Tissue: Stomach, Testis and Tongue.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT THR-898.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164; SER-905 AND SER-906, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
Voss M., Lettau M., Janssen O.
BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FASLG.
[7]"Characterization of the ubinuclein protein as a new member of the nuclear and adhesion complex components (NACos)."
Aho S., Lupo J., Coly P.-A., Sabine A., Castellazzi M., Morand P., Sergeant A., Manet E., Boyer V., Gruffat H.
Biol. Cell 101:319-334(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UBN1.
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164; SER-905 AND SER-906, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Crystal structure of the SH3-kinase fragment of tight junction protein 3 (TJP3) in apo-form."
Structural genomics consortium (SGC)
Submitted (NOV-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 475-775.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK091118 mRNA. Translation: BAG52285.1.
AK128237 mRNA. Translation: BAG54652.1.
AK304462 mRNA. Translation: BAG65278.1.
AC005954 Genomic DNA. Translation: AAC72274.1. Sequence problems.
AC006125 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69293.1. Sequence problems.
BC108906 mRNA. Translation: AAI08907.1.
RefSeqNP_001254489.1. NM_001267560.1.
NP_001254490.1. NM_001267561.1.
XP_005259596.1. XM_005259539.2.
UniGeneHs.25527.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3KFVX-ray2.80A475-775[»]
ProteinModelPortalO95049.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118025. 5 interactions.
IntActO95049. 3 interactions.
MINTMINT-210473.
STRING9606.ENSP00000262968.

PTM databases

PhosphoSiteO95049.

Proteomic databases

PaxDbO95049.
PRIDEO95049.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000382008; ENSP00000371438; ENSG00000105289. [O95049-1]
ENST00000539908; ENSP00000439991; ENSG00000105289.
ENST00000541714; ENSP00000439278; ENSG00000105289.
ENST00000587686; ENSP00000467864; ENSG00000105289. [O95049-3]
ENST00000589378; ENSP00000465419; ENSG00000105289. [O95049-4]
GeneID27134.
KEGGhsa:27134.
UCSCuc010xhu.3. human. [O95049-3]

Organism-specific databases

CTD27134.
GeneCardsGC19P003708.
H-InvDBHIX0014645.
HGNCHGNC:11829. TJP3.
HPACAB013244.
MIM612689. gene.
neXtProtNX_O95049.
PharmGKBPA36534.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG239704.
HOGENOMHOG000230923.
HOVERGENHBG017627.
InParanoidO95049.
KOK06097.
OMAQILKTCT.
PhylomeDBO95049.
TreeFamTF315606.

Gene expression databases

ArrayExpressO95049.
BgeeO95049.
CleanExHS_TJP3.
GenevestigatorO95049.

Family and domain databases

Gene3D2.30.42.10. 3 hits.
3.40.50.300. 1 hit.
InterProIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR005417. ZonOcculdens.
IPR005420. ZonOcculS3.
[Graphical view]
PANTHERPTHR13865:SF11. PTHR13865:SF11. 1 hit.
PfamPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSPR01597. ZONOCCLUDNS.
PR01600. ZONOCCLUDNS3.
SMARTSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEPS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO95049.
GeneWikiTJP3.
GenomeRNAi27134.
NextBio13603241.
PROO95049.
SOURCESearch...

Entry information

Entry nameZO3_HUMAN
AccessionPrimary (citable) accession number: O95049
Secondary accession number(s): A6NFP3 expand/collapse secondary AC list , B3KR73, B3KXZ0, B4E2W6, F5H2X0, F5H4S9, K7EK22, Q32N01
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: March 19, 2014
Last modified: April 16, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM