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Protein

Tight junction protein ZO-3

Gene

TJP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme and pathway databases

BioCyciZFISH:ENSG00000105289-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tight junction protein ZO-3
Alternative name(s):
Tight junction protein 3
Zona occludens protein 3
Zonula occludens protein 3
Gene namesi
Name:TJP3
Synonyms:ZO3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:11829. TJP3.

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

DisGeNETi27134.
OpenTargetsiENSG00000105289.
PharmGKBiPA36534.

Polymorphism and mutation databases

BioMutaiTJP3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945461 – 919Tight junction protein ZO-3Add BLAST919

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei112PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei169PhosphoserineBy similarity1
Modified residuei203PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei325PhosphothreonineCombined sources1
Modified residuei327PhosphoserineCombined sources1
Modified residuei371PhosphoserineCombined sources1
Modified residuei591PhosphoserineCombined sources1
Modified residuei856PhosphoserineCombined sources1
Modified residuei905PhosphoserineCombined sources1
Modified residuei906PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO95049.
MaxQBiO95049.
PaxDbiO95049.
PeptideAtlasiO95049.
PRIDEiO95049.

PTM databases

iPTMnetiO95049.
PhosphoSitePlusiO95049.

Expressioni

Gene expression databases

BgeeiENSG00000105289.
CleanExiHS_TJP3.
ExpressionAtlasiO95049. baseline and differential.
GenevisibleiO95049. HS.

Organism-specific databases

HPAiCAB013244.
HPA053337.

Interactioni

Subunit structurei

Interacts with occludin, claudins and ZO-1. Interacts with PATJ (By similarity). Interacts with UBN1. Interacts with FASLG.By similarity2 Publications

Protein-protein interaction databases

BioGridi118025. 7 interactors.
IntActiO95049. 4 interactors.
MINTiMINT-210473.
STRINGi9606.ENSP00000262968.

Structurei

Secondary structure

1919
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi478 – 482Combined sources5
Beta strandi490 – 493Combined sources4
Beta strandi501 – 506Combined sources6
Beta strandi522 – 527Combined sources6
Beta strandi533 – 539Combined sources7
Helixi542 – 555Combined sources14
Helixi596 – 602Combined sources7
Beta strandi606 – 614Combined sources9
Beta strandi621 – 625Combined sources5
Helixi628 – 638Combined sources11
Turni640 – 642Combined sources3
Beta strandi643 – 645Combined sources3
Helixi661 – 669Combined sources9
Beta strandi673 – 676Combined sources4
Helixi680 – 688Combined sources9
Beta strandi694 – 701Combined sources8
Helixi703 – 713Combined sources11
Helixi721 – 735Combined sources15
Helixi736 – 738Combined sources3
Beta strandi740 – 745Combined sources6
Beta strandi747 – 749Combined sources3
Helixi751 – 763Combined sources13
Beta strandi766 – 771Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KFVX-ray2.80A475-775[»]
ProteinModelPortaliO95049.
SMRiO95049.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95049.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 93PDZ 1PROSITE-ProRule annotationAdd BLAST83
Domaini195 – 272PDZ 2PROSITE-ProRule annotationAdd BLAST78
Domaini380 – 446PDZ 3PROSITE-ProRule annotationAdd BLAST67
Domaini475 – 549SH3PROSITE-ProRule annotationAdd BLAST75
Domaini580 – 761Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 3 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IQ5J. Eukaryota.
ENOG410XS08. LUCA.
GeneTreeiENSGT00840000129793.
HOGENOMiHOG000230923.
HOVERGENiHBG017627.
InParanoidiO95049.
KOiK06097.
OMAiTLYPGPG.
OrthoDBiEOG091G0AJZ.
TreeFamiTF315606.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR005417. ZO.
IPR005420. ZO-3.
[Graphical view]
PANTHERiPTHR13865:SF11. PTHR13865:SF11. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR01597. ZONOCCLUDNS.
PR01600. ZONOCCLUDNS3.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 5 (identifier: O95049-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEELTIWEQH TATLSKDPRR GFGIAISGGR DRPGGSMVVS DVVPGGPAEG
60 70 80 90 100
RLQTGDHIVM VNGVSMENAT SAFAIQILKT CTKMANITVK RPRRIHLPAT
110 120 130 140 150
KASPSSPGRQ DSDEDDGPQR VEEVDQGRGY DGDSSSGSGR SWDERSRRPR
160 170 180 190 200
PGRRGRAGSH GRRSPGGGSE ANGLALVSGF KRLPRQDVQM KPVKSVLVKR
210 220 230 240 250
RDSEEFGVKL GSQIFIKHIT DSGLAARHRG LQEGDLILQI NGVSSQNLSL
260 270 280 290 300
NDTRRLIEKS EGKLSLLVLR DRGQFLVNIP PAVSDSDSSP LEDISDLASE
310 320 330 340 350
LSQAPPSHIP PPPRHAQRSP EASQTDSPVE SPRLRRESSV DSRTISEPDE
360 370 380 390 400
QRSELPRESS YDIYRVPSSQ SMEDRGYSPD TRVVRFLKGK SIGLRLAGGN
410 420 430 440 450
DVGIFVSGVQ AGSPADGQGI QEGDQILQVN DVPFQNLTRE EAVQFLLGLP
460 470 480 490 500
PGEEMELVTQ RKQDIFWKMV QSRVGDSFYI RTHFELEPSP PSGLGFTRGD
510 520 530 540 550
VFHVLDTLHP GPGQSHARGG HWLAVRMGRD LREQERGIIP NQSRAEQLAS
560 570 580 590 600
LEAAQRAVGV GPGSSAGSNA RAEFWRLRGL RRGAKKTTQR SREDLSALTR
610 620 630 640 650
QGRYPPYERV VLREASFKRP VVILGPVADI AMQKLTAEMP DQFEIAETVS
660 670 680 690 700
RTDSPSKIIK LDTVRVIAEK DKHALLDVTP SAIERLNYVQ YYPIVVFFIP
710 720 730 740 750
ESRPALKALR QWLAPASRRS TRRLYAQAQK LRKHSSHLFT ATIPLNGTSD
760 770 780 790 800
TWYQELKAII REQQTRPIWT AEDQLDGSLE DNLDLPHHGL ADSSADLSCD
810 820 830 840 850
SRVNSDYETD GEGGAYTDGE GYTDGEGGPY TDVDDEPPAP ALARSSEPVQ
860 870 880 890 900
ADESQSPRDR GRISAHQGAQ VDSRHPQGQW RQDSMRTYER EALKKKFMRV
910
HDAESSDEDG YDWGPATDL
Length:919
Mass (Da):101,397
Last modified:March 19, 2014 - v3
Checksum:iD36EB684E7E4A6C8
GO
Isoform 3 (identifier: O95049-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNLCGLMPIFPAPLDQVADM

Note: No experimental confirmation available.
Show »
Length:938
Mass (Da):103,424
Checksum:i603411581EB7FA85
GO
Isoform 4 (identifier: O95049-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAVRFQVADM

Show »
Length:928
Mass (Da):102,415
Checksum:iB418537346F205CF
GO
Isoform 6 (identifier: O95049-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:883
Mass (Da):97,488
Checksum:i511564BFC624B3A8
GO

Sequence cautioni

The sequence AAC72274 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence EAW69293 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19R → H in AAI08907 (PubMed:15489334).Curated1
Sequence conflicti96H → L in BAG52285 (PubMed:14702039).Curated1
Sequence conflicti449L → P in BAG54652 (PubMed:14702039).Curated1
Sequence conflicti802R → H in BAG52285 (PubMed:14702039).Curated1
Sequence conflicti901H → R in BAG52285 (PubMed:14702039).Curated1
Sequence conflicti917T → M in BAG65278 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_056114898M → T.1 PublicationCorresponds to variant rs1046268dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0538441 – 36Missing in isoform 6. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_0402381M → MNLCGLMPIFPAPLDQVADM in isoform 3. 1 Publication1
Alternative sequenceiVSP_0470221M → MAVRFQVADM in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK091118 mRNA. Translation: BAG52285.1.
AK128237 mRNA. Translation: BAG54652.1.
AK304462 mRNA. Translation: BAG65278.1.
AC005954 Genomic DNA. Translation: AAC72274.1. Sequence problems.
AC006125 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69293.1. Sequence problems.
BC108906 mRNA. Translation: AAI08907.1.
CCDSiCCDS32873.2. [O95049-1]
CCDS59332.1. [O95049-4]
RefSeqiNP_001254489.1. NM_001267560.1. [O95049-1]
NP_001254490.1. NM_001267561.1. [O95049-4]
UniGeneiHs.25527.

Genome annotation databases

EnsembliENST00000539908; ENSP00000439991; ENSG00000105289. [O95049-5]
ENST00000541714; ENSP00000439278; ENSG00000105289. [O95049-1]
ENST00000587686; ENSP00000467864; ENSG00000105289. [O95049-3]
ENST00000589378; ENSP00000465419; ENSG00000105289. [O95049-4]
GeneIDi27134.
KEGGihsa:27134.
UCSCiuc010xhs.4. human. [O95049-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK091118 mRNA. Translation: BAG52285.1.
AK128237 mRNA. Translation: BAG54652.1.
AK304462 mRNA. Translation: BAG65278.1.
AC005954 Genomic DNA. Translation: AAC72274.1. Sequence problems.
AC006125 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69293.1. Sequence problems.
BC108906 mRNA. Translation: AAI08907.1.
CCDSiCCDS32873.2. [O95049-1]
CCDS59332.1. [O95049-4]
RefSeqiNP_001254489.1. NM_001267560.1. [O95049-1]
NP_001254490.1. NM_001267561.1. [O95049-4]
UniGeneiHs.25527.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KFVX-ray2.80A475-775[»]
ProteinModelPortaliO95049.
SMRiO95049.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118025. 7 interactors.
IntActiO95049. 4 interactors.
MINTiMINT-210473.
STRINGi9606.ENSP00000262968.

PTM databases

iPTMnetiO95049.
PhosphoSitePlusiO95049.

Polymorphism and mutation databases

BioMutaiTJP3.

Proteomic databases

EPDiO95049.
MaxQBiO95049.
PaxDbiO95049.
PeptideAtlasiO95049.
PRIDEiO95049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000539908; ENSP00000439991; ENSG00000105289. [O95049-5]
ENST00000541714; ENSP00000439278; ENSG00000105289. [O95049-1]
ENST00000587686; ENSP00000467864; ENSG00000105289. [O95049-3]
ENST00000589378; ENSP00000465419; ENSG00000105289. [O95049-4]
GeneIDi27134.
KEGGihsa:27134.
UCSCiuc010xhs.4. human. [O95049-1]

Organism-specific databases

CTDi27134.
DisGeNETi27134.
GeneCardsiTJP3.
H-InvDBHIX0014645.
HGNCiHGNC:11829. TJP3.
HPAiCAB013244.
HPA053337.
MIMi612689. gene.
neXtProtiNX_O95049.
OpenTargetsiENSG00000105289.
PharmGKBiPA36534.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQ5J. Eukaryota.
ENOG410XS08. LUCA.
GeneTreeiENSGT00840000129793.
HOGENOMiHOG000230923.
HOVERGENiHBG017627.
InParanoidiO95049.
KOiK06097.
OMAiTLYPGPG.
OrthoDBiEOG091G0AJZ.
TreeFamiTF315606.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000105289-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO95049.
GeneWikiiTJP3.
GenomeRNAii27134.
PROiO95049.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105289.
CleanExiHS_TJP3.
ExpressionAtlasiO95049. baseline and differential.
GenevisibleiO95049. HS.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR005417. ZO.
IPR005420. ZO-3.
[Graphical view]
PANTHERiPTHR13865:SF11. PTHR13865:SF11. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR01597. ZONOCCLUDNS.
PR01600. ZONOCCLUDNS3.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZO3_HUMAN
AccessioniPrimary (citable) accession number: O95049
Secondary accession number(s): A6NFP3
, B3KR73, B3KXZ0, B4E2W6, F5H2X0, F5H4S9, K7EK22, Q32N01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: March 19, 2014
Last modified: November 2, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.