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Protein

Rho guanine nucleotide exchange factor 15

Gene

ARHGEF15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific GEF for RhoA activation. Does not activate RAC1 or CDC42. Regulates vascular smooth muscle contractility. Negatively regulates excitatory synapse development by suppressing the synapse-promoting activity of EPHB2.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • Rho guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionGTPase activation, Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-HSA-193648 NRAGE signals death through JNK
R-HSA-194840 Rho GTPase cycle
R-HSA-416482 G alpha (12/13) signalling events
SignaLinkiO94989

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 15
Alternative name(s):
Ephexin-5
Short name:
E5
Vsm-RhoGEF
Gene namesi
Name:ARHGEF15
Synonyms:KIAA0915
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi

Organism-specific databases

EuPathDBiHostDB:ENSG00000198844.10
HGNCiHGNC:15590 ARHGEF15
MIMi608504 gene
neXtProtiNX_O94989

Subcellular locationi

Keywords - Cellular componenti

Cell projection

Pathology & Biotechi

Organism-specific databases

DisGeNETi22899
OpenTargetsiENSG00000198844
PharmGKBiPA24970

Polymorphism and mutation databases

BioMutaiARHGEF15

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809321 – 841Rho guanine nucleotide exchange factor 15Add BLAST841

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei107PhosphoserineBy similarity1
Modified residuei109PhosphoserineBy similarity1
Modified residuei353Phosphotyrosine; by EPHB2By similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues upon EFNA1 stimulation. EPHB2-dependent phosphorylation at Tyr-353 triggers UBE3A-mediated ubiquitination (By similarity).By similarity
Ubiquitinated; UBE3A-mediated ubiquitination and degradation by the proteasome promotes EFNB1-dependent synapse formation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO94989
PeptideAtlasiO94989
PRIDEiO94989

PTM databases

CarbonylDBiO94989
iPTMnetiO94989
PhosphoSitePlusiO94989

Expressioni

Tissue specificityi

Expressed in the vascular smooth muscle of coronary artery.1 Publication

Gene expression databases

BgeeiENSG00000198844
CleanExiHS_ARHGEF15
ExpressionAtlasiO94989 baseline and differential
GenevisibleiO94989 HS

Organism-specific databases

HPAiHPA044443
HPA063495

Interactioni

Subunit structurei

Interacts with EPHB2 (By similarity). Interacts with EPHA4.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP55Q53EZ45EBI-740691,EBI-747776

GO - Molecular functioni

  • Rho guanyl-nucleotide exchange factor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi116563, 8 interactors
IntActiO94989, 4 interactors
STRINGi9606.ENSP00000355026

Structurei

3D structure databases

ProteinModelPortaliO94989
SMRiO94989
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini417 – 601DHPROSITE-ProRule annotationAdd BLAST185

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 133Pro-richAdd BLAST127

Phylogenomic databases

eggNOGiKOG3523 Eukaryota
COG5422 LUCA
GeneTreeiENSGT00550000074493
HOGENOMiHOG000034047
HOVERGENiHBG103361
InParanoidiO94989
KOiK20687
OMAiTPRDHHT
OrthoDBiEOG091G04ML
PhylomeDBiO94989
TreeFamiTF316357

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR011993 PH-like_dom_sf
PfamiView protein in Pfam
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

O94989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQSLPAAT PPTQKPPRII RPRPPSRSRA AQSPGPPHNG SSPQELPRNS
60 70 80 90 100
NDAPTPMCTP IFWEPPAASL KPPALLPPSA SRASLDSQTS PDSPSSTPTP
110 120 130 140 150
SPVSRRSASP EPAPRSPVPP PKPSGSPCTP LLPMAGVLAQ NGSASAPGTV
160 170 180 190 200
RRLAGRFEGG AEGRAQDADA PEPGLQARAD VNGEREAPLT GSGSQENGAP
210 220 230 240 250
DAGLACPPCC PCVCHTTRPG LELRWVPVGG YEEVPRVPRR ASPLRTSRSR
260 270 280 290 300
PHPPSIGHPA VVLTSYRSTA ERKLLPLLKP PKPTRVRQDA TIFGDPPQPD
310 320 330 340 350
LDLLSEDGIQ TGDSPDEAPQ NTPPATVEGR EEEGLEVLKE QNWELPLQDE
360 370 380 390 400
PLYQTYRAAV LSEELWGVGE DGSPSPANAG DAPTFPRPPG PRNTLWQELP
410 420 430 440 450
AVQASGLLDT LSPQERRMQE SLFEVVTSEA SYLRSLRLLT DTFVLSQALR
460 470 480 490 500
DTLTPRDHHT LFSNVQRVQG VSERFLATLL SRVRSSPHIS DLCDVVHAHA
510 520 530 540 550
VGPFSVYVDY VRNQQYQEET YSRLMDTNVR FSAELRRLQS LPKCERLPLP
560 570 580 590 600
SFLLLPFQRI TRLRMLLQNI LRQTEEGSSR QENAQKALGA VSKIIERCSA
610 620 630 640 650
EVGRMKQTEE LIRLTQRLRF HKVKALPLVS WSRRLEFQGE LTELGCRRGG
660 670 680 690 700
VLFASRPRFT PLCLLLFSDL LLITQPKSGQ RLQVLDYAHR SLVQAQQVPD
710 720 730 740 750
PSGPPTFRLS LLSNHQGRPT HRLLQASSLS DMQRWLGAFP TPGPLPCSPD
760 770 780 790 800
TIYEDCDCSQ ELCSESSAPA KTEGRSLESR AAPKHLHKTP EGWLKGLPGA
810 820 830 840
FPAQLVCEVT GEHERRRHLR QNQRLLEAVG SSSGTPNAPP P
Length:841
Mass (Da):91,940
Last modified:January 20, 2009 - v4
Checksum:i40D430ACF777002F
GO

Sequence cautioni

The sequence BAA74938 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05718973P → S. Corresponds to variant dbSNP:rs9890841EnsemblClinVar.1
Natural variantiVAR_054215155G → V1 PublicationCorresponds to variant dbSNP:rs17857129Ensembl.1
Natural variantiVAR_054216277L → P3 PublicationsCorresponds to variant dbSNP:rs871841Ensembl.1
Natural variantiVAR_077835604R → C Found in a child with sporadic epilepsy; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs587777166EnsemblClinVar.1
Natural variantiVAR_054217831S → P4 PublicationsCorresponds to variant dbSNP:rs3744647Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020722 mRNA Translation: BAA74938.2 Different initiation.
AK291626 mRNA Translation: BAF84315.1
AC135178 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90060.1
BC036749 mRNA Translation: AAH36749.1
CCDSiCCDS11139.1
RefSeqiNP_079290.1, NM_025014.1
NP_776089.2, NM_173728.3
UniGeneiHs.443109

Genome annotation databases

EnsembliENST00000361926; ENSP00000355026; ENSG00000198844
ENST00000421050; ENSP00000412505; ENSG00000198844
GeneIDi22899
KEGGihsa:22899
UCSCiuc002glc.4 human

Keywords - Coding sequence diversityi

Polymorphism

Entry informationi

Entry nameiARHGF_HUMAN
AccessioniPrimary (citable) accession number: O94989
Secondary accession number(s): A8K6G1, Q8N449, Q9H8B4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 20, 2009
Last modified: March 28, 2018
This is version 139 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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