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Protein

Protein transport protein Sec31A

Gene

SEC31A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed:10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).By similarity1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-204005 COPII-mediated vesicle transport
R-HSA-2132295 MHC class II antigen presentation
R-HSA-381038 XBP1(S) activates chaperone genes
R-HSA-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC
SignaLinkiO94979

Protein family/group databases

TCDBi3.A.5.9.1 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein Sec31A
Alternative name(s):
ABP125
ABP130
SEC31-like protein 1
SEC31-related protein A
Web1-like protein
Gene namesi
Name:SEC31A
Synonyms:KIAA0905, SEC31L1
ORF Names:HSPC275, HSPC334
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000138674.16
HGNCiHGNC:17052 SEC31A
MIMi610257 gene
neXtProtiNX_O94979

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving SEC31A is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;4)(p23;q21) with ALK.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi647K → R: Does not abolish monoubiquitination by the BCR(KLHL12) E3 ubiquitin ligase complex, revealing flexibility of ubiquitination sites; when associated with R-1217. 1 Publication1
Mutagenesisi1217K → R: Does not abolish monoubiquitination by the BCR(KLHL12) E3 ubiquitin ligase complex, revealing flexibility of ubiquitination sites; when associated with R-647. 1 Publication1

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi22872
OpenTargetsiENSG00000138674
PharmGKBiPA162402737

Polymorphism and mutation databases

BioMutaiSEC31A

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951471 – 1220Protein transport protein Sec31AAdd BLAST1220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei527PhosphoserineCombined sources1
Modified residuei532PhosphoserineCombined sources1
Cross-linki647Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei799PhosphoserineCombined sources1
Modified residuei1161PhosphothreonineCombined sources1
Modified residuei1163PhosphoserineCombined sources1
Cross-linki1217Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats.2 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO94979
MaxQBiO94979
PaxDbiO94979
PeptideAtlasiO94979
PRIDEiO94979
ProteomicsDBi50595
50596 [O94979-2]
50597 [O94979-3]
50598 [O94979-4]
50599 [O94979-5]
50600 [O94979-6]
50601 [O94979-7]
50602 [O94979-8]

PTM databases

iPTMnetiO94979
PhosphoSitePlusiO94979
SwissPalmiO94979

Expressioni

Tissue specificityi

Abundantly and ubiquitously expressed.2 Publications

Gene expression databases

BgeeiENSG00000138674
ExpressionAtlasiO94979 baseline and differential
GenevisibleiO94979 HS

Organism-specific databases

HPAiHPA005457

Interactioni

Subunit structurei

COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13 (By similarity). Interacts with PDCD6; interaction takes place in response to cytosolic calcium increase and leads to bridge together the BCR(KLHL12) complex and SEC31A, leading to monoubiquitination (PubMed:27716508) (PubMed:16957052, PubMed:17196169, PubMed:27716508, PubMed:25667979). Interacts with KLHL12.By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116539, 54 interactors
DIPiDIP-40438N
IntActiO94979, 37 interactors
MINTiO94979
STRINGi9606.ENSP00000347329

Structurei

Secondary structure

11220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi841 – 843Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WXAX-ray2.36C/D837-848[»]
ProteinModelPortaliO94979
SMRiO94979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 47WD 1Add BLAST44
Repeati68 – 111WD 2Add BLAST44
Repeati120 – 160WD 3Add BLAST41
Repeati166 – 206WD 4Add BLAST41
Repeati209 – 254WD 5Add BLAST46
Repeati258 – 298WD 6Add BLAST41
Repeati301 – 342WD 7Add BLAST42
Repeati397 – 430WD 8; interaction with SEC13PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 471Interaction with SEC131 PublicationAdd BLAST311
Regioni800 – 1113Interaction with PDCD61 PublicationAdd BLAST314

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi842 – 848ALG-2-binding site motif-2 (ABS-2),7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi800 – 1091Pro-richAdd BLAST292

Domaini

The ALG-2-binding site motif-2 (ABS-2) contains a PXPGF sequence that binds hydrophobic pocket 3 of PDCD6.1 Publication

Sequence similaritiesi

Belongs to the WD repeat SEC31 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0307 Eukaryota
ENOG410XQ1D LUCA
GeneTreeiENSGT00390000003175
HOGENOMiHOG000230582
HOVERGENiHBG055626
InParanoidiO94979
KOiK14005
PhylomeDBiO94979
TreeFamiTF313842

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024298 ACE1_Sec16_Sec31
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12931 Sec16_C, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (10)i

Sequence statusi: Complete.

This entry describes 10 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O94979-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLKEVDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL
60 70 80 90 100
SDPSLDMKSC ATFSSSHRYH KLIWGPYKMD SKGDVSGVLI AGGENGNIIL
110 120 130 140 150
YDPSKIIAGD KEVVIAQNDK HTGPVRALDV NIFQTNLVAS GANESEIYIW
160 170 180 190 200
DLNNFATPMT PGAKTQPPED ISCIAWNRQV QHILASASPS GRATVWDLRK
210 220 230 240 250
NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV IQMWDLRFAS
260 270 280 290 300
SPLRVLENHA RGILAIAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP
310 320 330 340 350
TNTQWCFDIQ WCPRNPAVLS AASFDGRISV YSIMGGSTDG LRQKQVDKLS
360 370 380 390 400
SSFGNLDPFG TGQPLPPLQI PQQTAQHSIV LPLKKPPKWI RRPVGASFSF
410 420 430 440 450
GGKLVTFENV RMPSHQGAEQ QQQQHHVFIS QVVTEKEFLS RSDQLQQAVQ
460 470 480 490 500
SQGFINYCQK KIDASQTEFE KNVWSFLKVN FEDDSRGKYL ELLGYRKEDL
510 520 530 540 550
GKKIALALNK VDGANVALKD SDQVAQSDGE ESPAAEEQLL GEHIKEEKEE
560 570 580 590 600
SEFLPSSGGT FNISVSGDID GLITQALLTG NFESAVDLCL HDNRMADAII
610 620 630 640 650
LAIAGGQELL ARTQKKYFAK SQSKITRLIT AVVMKNWKEI VESCDLKNWR
660 670 680 690 700
EALAAVLTYA KPDEFSALCD LLGTRLENEG DSLLQTQACL CYICAGNVEK
710 720 730 740 750
LVACWTKAQD GSHPLSLQDL IEKVVILRKA VQLTQAMDTS TVGVLLAAKM
760 770 780 790 800
SQYANLLAAQ GSIAAALAFL PDNTNQPNIM QLRDRLCRAQ GEPVAGHESP
810 820 830 840 850
KIPYEKQQLP KGRPGPVAGH HQMPRVQTQQ YYPHGENPPP PGFIMHGNVN
860 870 880 890 900
PNAAGQLPTS PGHMHTQVPP YPQPQPYQPA QPYPFGTGGS AMYRPQQPVA
910 920 930 940 950
PPTSNAYPNT PYISSASSYT GQSQLYAAQH QASSPTSSPA TSFPPPPSSG
960 970 980 990 1000
ASFQHGGPGA PPSSSAYALP PGTTGTLPAA SELPASQRTG PQNGWNDPPA
1010 1020 1030 1040 1050
LNRVPKKKKM PENFMPPVPI TSPIMNPLGD PQSQMLQQQP SAPVPLSSQS
1060 1070 1080 1090 1100
SFPQPHLPGG QPFHGVQQPL GQTGMPPSFS KPNIEGAPGA PIGNTFQHVQ
1110 1120 1130 1140 1150
SLPTKKITKK PIPDEHLILK TTFEDLIQRC LSSATDPQTK RKLDDASKRL
1160 1170 1180 1190 1200
EFLYDKLREQ TLSPTITSGL HNIARSIETR NYSEGLTMHT HIVSTSNFSE
1210 1220
TSAFMPVLKV VLTQANKLGV
Length:1,220
Mass (Da):133,015
Last modified:July 10, 2007 - v3
Checksum:i2A633B4436DB7482
GO
Isoform 2 (identifier: O94979-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     974-988: Missing.

Show »
Length:1,205
Mass (Da):131,534
Checksum:i245E11A9AD589F74
GO
Isoform 3 (identifier: O94979-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     876-876: P → R
     877-990: Missing.

Show »
Length:1,106
Mass (Da):121,651
Checksum:iD21AB6B647E1291B
GO
Isoform 4 (identifier: O94979-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-542: Missing.

Show »
Length:1,181
Mass (Da):129,036
Checksum:i6FCDB50DE1A19BF0
GO
Isoform 5 (identifier: O94979-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-509: IALALN → VNFWES
     510-1220: Missing.

Note: No experimental confirmation available.
Show »
Length:509
Mass (Da):56,731
Checksum:i883FD8815650FA25
GO
Isoform 6 (identifier: O94979-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-542: Missing.
     876-876: P → R
     877-990: Missing.

Show »
Length:1,067
Mass (Da):117,673
Checksum:iF2D05279DCE51522
GO
Isoform 7 (identifier: O94979-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-228: Missing.
     229-260: MVLASEDDRLPVIQMWDLRFASSPLRVLENHA → MVKLVLLSIVLLKVTVPKLSNYLLQLDFMPIH
     504-542: Missing.
     834-834: H → HVRIAPTVTTWSNKTPTALPSHPPAASPSDTQ
     974-988: Missing.

Note: No experimental confirmation available.
Show »
Length:969
Mass (Da):105,753
Checksum:iC968C7ACE341CCAB
GO
Isoform 8 (identifier: O94979-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     989-989: T → TENQSIQDQAPMLE

Show »
Length:1,233
Mass (Da):134,499
Checksum:i1D63F1DEA3EC45F4
GO
Isoform 9 (identifier: O94979-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MKLKEVDRTAMQAWSPAQNHPIYLAT → MLGESDERCTNAGSGCRRSSP
     974-988: Missing.

Note: No experimental confirmation available.
Show »
Length:1,200
Mass (Da):130,747
Checksum:iF2E4A99D905A6389
GO
Isoform 10 (identifier: O94979-10) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-542: Missing.
     974-988: Missing.

Note: No experimental confirmation available.
Show »
Length:1,166
Mass (Da):127,556
Checksum:iC780B386B096906E
GO

Sequence cautioni

The sequence AAF28953 differs from that shown. Reason: Frameshift at positions 857 and 1079.Curated
The sequence AAF29012 differs from that shown. Reason: Frameshift at positions 922, 946, 1029 and 1080.Curated
The sequence CAI45995 differs from that shown. Reason: Erroneous termination at position 221. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200K → E in BAA84923 (Ref. 2) Curated1
Sequence conflicti284K → R in BAC86336 (PubMed:14702039).Curated1
Sequence conflicti854A → S in BAA84923 (Ref. 2) Curated1
Sequence conflicti854A → S in BAA84924 (Ref. 2) Curated1
Sequence conflicti1007K → R in BAG58628 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_033225263I → V. Corresponds to variant dbSNP:rs34554214Ensembl.1
Natural variantiVAR_053414456N → K. Corresponds to variant dbSNP:rs3797036Ensembl.1
Natural variantiVAR_033226841P → L. Corresponds to variant dbSNP:rs35579207Ensembl.1
Natural variantiVAR_0332271055P → T. Corresponds to variant dbSNP:rs35739017Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0267421 – 228Missing in isoform 7. 1 PublicationAdd BLAST228
Alternative sequenceiVSP_0446021 – 26MKLKE…IYLAT → MLGESDERCTNAGSGCRRSS P in isoform 9. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_026743229 – 260MVLAS…LENHA → MVKLVLLSIVLLKVTVPKLS NYLLQLDFMPIH in isoform 7. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_026744504 – 542Missing in isoform 4, isoform 6, isoform 7 and isoform 10. 4 PublicationsAdd BLAST39
Alternative sequenceiVSP_026745504 – 509IALALN → VNFWES in isoform 5. 1 Publication6
Alternative sequenceiVSP_026746510 – 1220Missing in isoform 5. 1 PublicationAdd BLAST711
Alternative sequenceiVSP_026747834H → HVRIAPTVTTWSNKTPTALP SHPPAASPSDTQ in isoform 7. 1 Publication1
Alternative sequenceiVSP_026748876P → R in isoform 3 and isoform 6. 2 Publications1
Alternative sequenceiVSP_026749877 – 990Missing in isoform 3 and isoform 6. 2 PublicationsAdd BLAST114
Alternative sequenceiVSP_026750974 – 988Missing in isoform 2, isoform 7, isoform 9 and isoform 10. 2 PublicationsAdd BLAST15
Alternative sequenceiVSP_026751989T → TENQSIQDQAPMLE in isoform 8. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139184 mRNA Translation: AAF67836.1
AB018358 mRNA Translation: BAA84923.1
AB018359 mRNA Translation: BAA84924.1
AB020712 mRNA Translation: BAA74928.2
AK125897 mRNA Translation: BAC86336.1
AK295810 mRNA Translation: BAG58628.1
AL049463 mRNA Translation: CAH56418.1
CR933696 mRNA Translation: CAI45995.1 Sequence problems.
AC021105 Genomic DNA No translation available.
AC108469 Genomic DNA No translation available.
CH471057 Genomic DNA Translation: EAX05908.1
BC047883 mRNA Translation: AAH47883.1
BC084583 mRNA Translation: AAH84583.1
BC117221 mRNA Translation: AAI17222.1
BC143489 mRNA Translation: AAI43490.1
BC143491 mRNA Translation: AAI43492.1
BC143492 mRNA Translation: AAI43493.1
AY137583 mRNA Translation: AAN15221.1
AF161393 mRNA Translation: AAF28953.1 Frameshift.
AF161452 mRNA Translation: AAF29012.1 Frameshift.
CCDSiCCDS3596.1 [O94979-1]
CCDS3597.1 [O94979-4]
CCDS43244.1 [O94979-3]
CCDS47088.1 [O94979-2]
CCDS54773.1 [O94979-9]
CCDS75155.1 [O94979-6]
CCDS75156.1 [O94979-10]
RefSeqiNP_001070674.1, NM_001077206.3 [O94979-3]
NP_001070675.1, NM_001077207.3 [O94979-1]
NP_001070676.1, NM_001077208.3 [O94979-2]
NP_001177978.1, NM_001191049.2 [O94979-9]
NP_001287673.1, NM_001300744.2 [O94979-6]
NP_001287674.1, NM_001300745.2 [O94979-10]
NP_001305048.1, NM_001318119.1 [O94979-2]
NP_001305049.1, NM_001318120.1 [O94979-1]
NP_057295.2, NM_016211.4 [O94979-4]
UniGeneiHs.370024
Hs.617723
Hs.728916

Genome annotation databases

EnsembliENST00000311785; ENSP00000309070; ENSG00000138674 [O94979-3]
ENST00000348405; ENSP00000337602; ENSG00000138674 [O94979-4]
ENST00000355196; ENSP00000347329; ENSG00000138674 [O94979-1]
ENST00000395310; ENSP00000378721; ENSG00000138674 [O94979-1]
ENST00000443462; ENSP00000408027; ENSG00000138674 [O94979-9]
ENST00000448323; ENSP00000400926; ENSG00000138674 [O94979-1]
ENST00000500777; ENSP00000421464; ENSG00000138674 [O94979-6]
ENST00000505984; ENSP00000424451; ENSG00000138674 [O94979-10]
ENST00000508502; ENSP00000424635; ENSG00000138674 [O94979-2]
ENST00000509142; ENSP00000426569; ENSG00000138674 [O94979-3]
ENST00000513858; ENSP00000426886; ENSG00000138674 [O94979-6]
GeneIDi22872
KEGGihsa:22872
UCSCiuc003hnf.3 human [O94979-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSC31A_HUMAN
AccessioniPrimary (citable) accession number: O94979
Secondary accession number(s): B4DIW6
, B7ZKZ7, B7ZL00, H7C2W3, Q17RR5, Q5H9P6, Q5XG74, Q659G7, Q6ZU90, Q7LCX9, Q86TJ0, Q8IZH4, Q9P048, Q9P0A6, Q9UM05, Q9UM06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: June 20, 2018
This is version 157 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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