UniProtKB - O94979 (SC31A_HUMAN)
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Protein
Protein transport protein Sec31A
Gene
SEC31A
Organism
Homo sapiens (Human)
Status
Functioni
Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed:10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).By similarity1 Publication
GO - Molecular functioni
- calcium-dependent protein binding Source: UniProtKB
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
- COPII vesicle coating Source: Reactome
- ER to Golgi vesicle-mediated transport Source: UniProtKB
- IRE1-mediated unfolded protein response Source: Reactome
- protein transport Source: UniProtKB-KW
- response to calcium ion Source: UniProtKB
Keywordsi
| Biological process | ER-Golgi transport, Protein transport, Transport |
Enzyme and pathway databases
| Reactomei | R-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport. R-HSA-2132295. MHC class II antigen presentation. R-HSA-381038. XBP1(S) activates chaperone genes. R-HSA-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC. |
| SignaLinki | O94979. |
Protein family/group databases
| TCDBi | 3.A.5.9.1. the general secretory pathway (sec) family. |
Names & Taxonomyi
| Protein namesi | Recommended name: Protein transport protein Sec31AAlternative name(s): ABP125 ABP130 SEC31-like protein 1 SEC31-related protein A Web1-like protein |
| Gene namesi | Name:SEC31A Synonyms:KIAA0905, SEC31L1 ORF Names:HSPC275, HSPC334 |
| Organismi | Homo sapiens (Human) |
| Taxonomic identifieri | 9606 [NCBI] |
| Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
| Proteomesi |
|
Organism-specific databases
| HGNCi | HGNC:17052. SEC31A. |
Subcellular locationi
- Cytoplasm By similarity
- Cytoplasmic vesicle › COPII-coated vesicle membrane 4 Publications; Peripheral membrane protein; Cytoplasmic side
- Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
Note: Associates with membranes in a GTP-dependent manner.By similarity
GO - Cellular componenti
- COPII vesicle coat Source: UniProtKB
- cytoplasm Source: UniProtKB
- cytosol Source: HPA
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum exit site Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
- ER to Golgi transport vesicle Source: UniProtKB
- ER to Golgi transport vesicle membrane Source: Reactome
- Golgi membrane Source: GOC
- intracellular membrane-bounded organelle Source: HPA
- perinuclear region of cytoplasm Source: UniProtKB
- vesicle coat Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, MembranePathology & Biotechi
Involvement in diseasei
A chromosomal aberration involving SEC31A is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;4)(p23;q21) with ALK.
Mutagenesis
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Mutagenesisi | 647 | K → R: Does not abolish monoubiquitination by the BCR(KLHL12) E3 ubiquitin ligase complex, revealing flexibility of ubiquitination sites; when associated with R-1217. 1 Publication | 1 | |
| Mutagenesisi | 1217 | K → R: Does not abolish monoubiquitination by the BCR(KLHL12) E3 ubiquitin ligase complex, revealing flexibility of ubiquitination sites; when associated with R-647. 1 Publication | 1 |
Keywords - Diseasei
Proto-oncogeneOrganism-specific databases
| DisGeNETi | 22872. |
| OpenTargetsi | ENSG00000138674. |
| PharmGKBi | PA162402737. |
Polymorphism and mutation databases
| BioMutai | SEC31A. |
PTM / Processingi
Molecule processing
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| ChainiPRO_0000295147 | 1 – 1220 | Protein transport protein Sec31AAdd BLAST | 1220 |
Amino acid modifications
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Modified residuei | 527 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 532 | PhosphoserineCombined sources | 1 | |
| Cross-linki | 647 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
| Modified residuei | 799 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 1161 | PhosphothreonineCombined sources | 1 | |
| Modified residuei | 1163 | PhosphoserineCombined sources | 1 | |
| Cross-linki | 1217 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats.2 Publications
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
| EPDi | O94979. |
| MaxQBi | O94979. |
| PaxDbi | O94979. |
| PeptideAtlasi | O94979. |
| PRIDEi | O94979. |
PTM databases
| iPTMneti | O94979. |
| PhosphoSitePlusi | O94979. |
| SwissPalmi | O94979. |
Expressioni
Tissue specificityi
Abundantly and ubiquitously expressed.2 Publications
Gene expression databases
| Bgeei | ENSG00000138674. |
| ExpressionAtlasi | O94979. baseline and differential. |
| Genevisiblei | O94979. HS. |
Organism-specific databases
| HPAi | HPA005457. |
Interactioni
Subunit structurei
COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13 (By similarity). Interacts with PDCD6; interaction takes place in response to cytosolic calcium increase and leads to bridge together the BCR(KLHL12) complex and SEC31A, leading to monoubiquitination (PubMed:27716508) (PubMed:16957052, PubMed:17196169, PubMed:27716508, PubMed:25667979). Interacts with KLHL12.By similarity4 Publications
Binary interactionsi
GO - Molecular functioni
- calcium-dependent protein binding Source: UniProtKB
Protein-protein interaction databases
| BioGridi | 116539. 52 interactors. |
| DIPi | DIP-40438N. |
| IntActi | O94979. 35 interactors. |
| MINTi | MINT-3002555. |
| STRINGi | 9606.ENSP00000347329. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Beta strandi | 841 – 843 | Combined sources | 3 |
3D structure databases
| Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
| 3WXA | X-ray | 2.36 | C/D | 837-848 | [»] | |
| ProteinModelPortali | O94979. | |||||
| SMRi | O94979. | |||||
| ModBasei | Search... | |||||
| MobiDBi | Search... | |||||
Family & Domainsi
Domains and Repeats
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Repeati | 4 – 47 | WD 1Add BLAST | 44 | |
| Repeati | 68 – 111 | WD 2Add BLAST | 44 | |
| Repeati | 120 – 160 | WD 3Add BLAST | 41 | |
| Repeati | 166 – 206 | WD 4Add BLAST | 41 | |
| Repeati | 209 – 254 | WD 5Add BLAST | 46 | |
| Repeati | 258 – 298 | WD 6Add BLAST | 41 | |
| Repeati | 301 – 342 | WD 7Add BLAST | 42 | |
| Repeati | 397 – 430 | WD 8; interaction with SEC13PROSITE-ProRule annotationAdd BLAST | 34 |
Region
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Regioni | 161 – 471 | Interaction with SEC131 PublicationAdd BLAST | 311 | |
| Regioni | 800 – 1113 | Interaction with PDCD61 PublicationAdd BLAST | 314 |
Motif
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Motifi | 842 – 848 | ALG-2-binding site motif-2 (ABS-2), | 7 |
Compositional bias
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Compositional biasi | 800 – 1091 | Pro-richAdd BLAST | 292 |
Domaini
The ALG-2-binding site motif-2 (ABS-2) contains a PXPGF sequence that binds hydrophobic pocket 3 of PDCD6.1 Publication
Sequence similaritiesi
Belongs to the WD repeat SEC31 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
| eggNOGi | KOG0307. Eukaryota. ENOG410XQ1D. LUCA. |
| GeneTreei | ENSGT00390000003175. |
| HOGENOMi | HOG000230582. |
| HOVERGENi | HBG055626. |
| InParanoidi | O94979. |
| KOi | K14005. |
| PhylomeDBi | O94979. |
| TreeFami | TF313842. |
Family and domain databases
| Gene3Di | 2.130.10.10. 1 hit. |
| InterProi | View protein in InterPro IPR024298. ACE1_Sec16_Sec31. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. |
| Pfami | View protein in Pfam PF12931. Sec16_C. 1 hit. |
| SMARTi | View protein in SMART SM00320. WD40. 6 hits. |
| SUPFAMi | SSF50978. SSF50978. 1 hit. |
| PROSITEi | View protein in PROSITE PS50082. WD_REPEATS_2. 1 hit. PS50294. WD_REPEATS_REGION. 1 hit. |
Sequences (10)i
Sequence statusi: Complete.
This entry describes 10 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O94979-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MKLKEVDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL
60 70 80 90 100
SDPSLDMKSC ATFSSSHRYH KLIWGPYKMD SKGDVSGVLI AGGENGNIIL
110 120 130 140 150
YDPSKIIAGD KEVVIAQNDK HTGPVRALDV NIFQTNLVAS GANESEIYIW
160 170 180 190 200
DLNNFATPMT PGAKTQPPED ISCIAWNRQV QHILASASPS GRATVWDLRK
210 220 230 240 250
NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV IQMWDLRFAS
260 270 280 290 300
SPLRVLENHA RGILAIAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP
310 320 330 340 350
TNTQWCFDIQ WCPRNPAVLS AASFDGRISV YSIMGGSTDG LRQKQVDKLS
360 370 380 390 400
SSFGNLDPFG TGQPLPPLQI PQQTAQHSIV LPLKKPPKWI RRPVGASFSF
410 420 430 440 450
GGKLVTFENV RMPSHQGAEQ QQQQHHVFIS QVVTEKEFLS RSDQLQQAVQ
460 470 480 490 500
SQGFINYCQK KIDASQTEFE KNVWSFLKVN FEDDSRGKYL ELLGYRKEDL
510 520 530 540 550
GKKIALALNK VDGANVALKD SDQVAQSDGE ESPAAEEQLL GEHIKEEKEE
560 570 580 590 600
SEFLPSSGGT FNISVSGDID GLITQALLTG NFESAVDLCL HDNRMADAII
610 620 630 640 650
LAIAGGQELL ARTQKKYFAK SQSKITRLIT AVVMKNWKEI VESCDLKNWR
660 670 680 690 700
EALAAVLTYA KPDEFSALCD LLGTRLENEG DSLLQTQACL CYICAGNVEK
710 720 730 740 750
LVACWTKAQD GSHPLSLQDL IEKVVILRKA VQLTQAMDTS TVGVLLAAKM
760 770 780 790 800
SQYANLLAAQ GSIAAALAFL PDNTNQPNIM QLRDRLCRAQ GEPVAGHESP
810 820 830 840 850
KIPYEKQQLP KGRPGPVAGH HQMPRVQTQQ YYPHGENPPP PGFIMHGNVN
860 870 880 890 900
PNAAGQLPTS PGHMHTQVPP YPQPQPYQPA QPYPFGTGGS AMYRPQQPVA
910 920 930 940 950
PPTSNAYPNT PYISSASSYT GQSQLYAAQH QASSPTSSPA TSFPPPPSSG
960 970 980 990 1000
ASFQHGGPGA PPSSSAYALP PGTTGTLPAA SELPASQRTG PQNGWNDPPA
1010 1020 1030 1040 1050
LNRVPKKKKM PENFMPPVPI TSPIMNPLGD PQSQMLQQQP SAPVPLSSQS
1060 1070 1080 1090 1100
SFPQPHLPGG QPFHGVQQPL GQTGMPPSFS KPNIEGAPGA PIGNTFQHVQ
1110 1120 1130 1140 1150
SLPTKKITKK PIPDEHLILK TTFEDLIQRC LSSATDPQTK RKLDDASKRL
1160 1170 1180 1190 1200
EFLYDKLREQ TLSPTITSGL HNIARSIETR NYSEGLTMHT HIVSTSNFSE
1210 1220
TSAFMPVLKV VLTQANKLGV
Isoform 7 (identifier: O94979-7) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-228: Missing.
229-260: MVLASEDDRLPVIQMWDLRFASSPLRVLENHA → MVKLVLLSIVLLKVTVPKLSNYLLQLDFMPIH
504-542: Missing.
834-834: H → HVRIAPTVTTWSNKTPTALPSHPPAASPSDTQ
974-988: Missing.
Note: No experimental confirmation available.
Show »Isoform 10 (identifier: O94979-10) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
504-542: Missing.
974-988: Missing.
Note: No experimental confirmation available.
Show »Sequence cautioni
The sequence AAF29012 differs from that shown. Reason: Frameshift at positions 922, 946, 1029 and 1080.Curated
The sequence CAI45995 differs from that shown. Reason: Erroneous termination at position 221. Translated as Trp.Curated
Experimental Info
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Sequence conflicti | 200 | K → E in BAA84923 (Ref. 2) Curated | 1 | |
| Sequence conflicti | 284 | K → R in BAC86336 (PubMed:14702039).Curated | 1 | |
| Sequence conflicti | 854 | A → S in BAA84923 (Ref. 2) Curated | 1 | |
| Sequence conflicti | 854 | A → S in BAA84924 (Ref. 2) Curated | 1 | |
| Sequence conflicti | 1007 | K → R in BAG58628 (PubMed:14702039).Curated | 1 |
Natural variant
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Natural variantiVAR_033225 | 263 | I → V. Corresponds to variant dbSNP:rs34554214Ensembl. | 1 | |
| Natural variantiVAR_053414 | 456 | N → K. Corresponds to variant dbSNP:rs3797036Ensembl. | 1 | |
| Natural variantiVAR_033226 | 841 | P → L. Corresponds to variant dbSNP:rs35579207Ensembl. | 1 | |
| Natural variantiVAR_033227 | 1055 | P → T. Corresponds to variant dbSNP:rs35739017Ensembl. | 1 |
Alternative sequence
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Alternative sequenceiVSP_026742 | 1 – 228 | Missing in isoform 7. 1 PublicationAdd BLAST | 228 | |
| Alternative sequenceiVSP_044602 | 1 – 26 | MKLKE…IYLAT → MLGESDERCTNAGSGCRRSS P in isoform 9. 1 PublicationAdd BLAST | 26 | |
| Alternative sequenceiVSP_026743 | 229 – 260 | MVLAS…LENHA → MVKLVLLSIVLLKVTVPKLS NYLLQLDFMPIH in isoform 7. 1 PublicationAdd BLAST | 32 | |
| Alternative sequenceiVSP_026744 | 504 – 542 | Missing in isoform 4, isoform 6, isoform 7 and isoform 10. 4 PublicationsAdd BLAST | 39 | |
| Alternative sequenceiVSP_026745 | 504 – 509 | IALALN → VNFWES in isoform 5. 1 Publication | 6 | |
| Alternative sequenceiVSP_026746 | 510 – 1220 | Missing in isoform 5. 1 PublicationAdd BLAST | 711 | |
| Alternative sequenceiVSP_026747 | 834 | H → HVRIAPTVTTWSNKTPTALP SHPPAASPSDTQ in isoform 7. 1 Publication | 1 | |
| Alternative sequenceiVSP_026748 | 876 | P → R in isoform 3 and isoform 6. 2 Publications | 1 | |
| Alternative sequenceiVSP_026749 | 877 – 990 | Missing in isoform 3 and isoform 6. 2 PublicationsAdd BLAST | 114 | |
| Alternative sequenceiVSP_026750 | 974 – 988 | Missing in isoform 2, isoform 7, isoform 9 and isoform 10. 2 PublicationsAdd BLAST | 15 | |
| Alternative sequenceiVSP_026751 | 989 | T → TENQSIQDQAPMLE in isoform 8. 1 Publication | 1 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicing, Chromosomal rearrangement, PolymorphismSimilar proteinsi
Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:| 100% | UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry. |
| 90% | UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence). |
| 50% | UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster. |
Entry informationi
| Entry namei | SC31A_HUMAN | |
| Accessioni | O94979Primary (citable) accession number: O94979 Secondary accession number(s): B4DIW6 Q9UM06 | |
| Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 10, 2007 |
| Last sequence update: | July 10, 2007 | |
| Last modified: | June 7, 2017 | |
| This is version 147 of the entry and version 3 of the sequence. See complete history. | ||
| Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
| Annotation program | Chordata Protein Annotation Program | |
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families