O94956 (SO2B1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier organic anion transporter family member 2B1

Alternative name(s):
Organic anion transporter B
Short name=OATP-B
Organic anion transporter polypeptide-related protein 2
Short name=OATP-RP2
Short name=OATPRP2
Solute carrier family 21 member 9

Gene names

Name:	SLCO2B1
Synonyms:	KIAA0880, OATP2B1, OATPB, SLC21A9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the Na+-independent transport of organic anions such as taurocholate, the prostaglandins PGD2, PGE1, PGE2, leukotriene C4, thromboxane B2 and iloprost By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the organo anion transporter (TC 2.A.60) family. [View classification] Contains 1 Kazal-like domain.
Sequence caution	The sequence AAG42205.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAA74903.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sodium-independent organic anion transport Traceable author statement. Source: Reactome
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	sodium-independent organic anion transmembrane transporter activity Inferred from direct assay PubMed 18501590. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94956-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94956-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     150-260: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 709	709	Solute carrier organic anion transporter family member 2B1		PRO_0000191061
Regions
	Topological domain	1 – 49	49	Cytoplasmic Potential
	Transmembrane	50 – 69	20	Helical; Name=1; Potential
	Topological domain	70 – 88	19	Extracellular Potential
	Transmembrane	89 – 109	21	Helical; Name=2; Potential
	Topological domain	110 – 115	6	Cytoplasmic Potential
	Transmembrane	116 – 140	25	Helical; Name=3; Potential
	Topological domain	141 – 185	45	Extracellular Potential
	Transmembrane	186 – 215	30	Helical; Name=4; Potential
	Topological domain	216 – 234	19	Cytoplasmic Potential
	Transmembrane	235 – 255	21	Helical; Name=5; Potential
	Topological domain	256 – 273	18	Extracellular Potential
	Transmembrane	274 – 298	25	Helical; Name=6; Potential
	Topological domain	299 – 366	68	Cytoplasmic Potential
	Transmembrane	367 – 388	22	Helical; Name=7; Potential
	Topological domain	389 – 408	20	Extracellular Potential
	Transmembrane	409 – 432	24	Helical; Name=8; Potential
	Topological domain	433 – 436	4	Cytoplasmic Potential
	Transmembrane	437 – 460	24	Helical; Name=9; Potential
	Topological domain	461 – 564	104	Extracellular Potential
	Transmembrane	565 – 587	23	Helical; Name=10; Potential
	Topological domain	588 – 596	9	Cytoplasmic Potential
	Transmembrane	597 – 622	26	Helical; Name=11; Potential
	Topological domain	623 – 655	33	Extracellular Potential
	Transmembrane	656 – 673	18	Helical; Name=12; Potential
	Topological domain	674 – 709	36	Cytoplasmic Potential
	Domain	483 – 543	61	Kazal-like
Amino acid modifications
	Modified residue	34	1	Phosphoserine By similarity
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	538	1	N-linked (GlcNAc...) Potential
	Disulfide bond	489 ↔ 516		By similarity
	Disulfide bond	493 ↔ 504		By similarity
	Disulfide bond	495 ↔ 520		By similarity
Natural variations
	Alternative sequence	1 – 116	116	Missing in isoform 2.		VSP_006147
	Alternative sequence	150 – 260	111	Missing in isoform 2.		VSP_006148
	Natural variant	77	1	E → K in a breast cancer sample; somatic mutation. Ref.8		VAR_036412
	Natural variant	201	1	V → M. Corresponds to variant rs35199625 [ dbSNP | Ensembl ].		VAR_053675
	Natural variant	312	1	R → Q. Corresponds to variant rs12422149 [ dbSNP | Ensembl ].		VAR_053676
	Natural variant	392	1	I → T. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6 Corresponds to variant rs1621378 [ dbSNP | Ensembl ].		VAR_053677
	Natural variant	486	1	S → F. Corresponds to variant rs2306168 [ dbSNP | Ensembl ].		VAR_020294
Experimental info
	Sequence conflict	215	1	D → V Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 652DB4B70B2D9A2D	FASTA	709	76,711
	10 20 30 40 50 60 MGPRIGPAGE VPQVPDKETK ATMGTENTPG GKASPDPQDV RPSVFHNIKL FVLCHSLLQL 70 80 90 100 110 120 AQLMISGYLK SSISTVEKRF GLSSQTSGLL ASFNEVGNTA LIVFVSYFGS RVHRPRMIGY 130 140 150 160 170 180 GAILVALAGL LMTLPHFISE PYRYDNTSPE DMPQDFKASL CLPTTSAPAS APSNGNCSSY 190 200 210 220 230 240 TETQHLSVVG IMFVAQTLLG VGGVPIQPFG ISYIDDFAHN SNSPLYLGIL FAVTMMGPGL 250 260 270 280 290 300 AFGLGSLMLR LYVDINQMPE GGISLTIKDP RWVGAWWLGF LIAAGAVALA AIPYFFFPKE 310 320 330 340 350 360 MPKEKRELQF RRKVLAVTDS PARKGKDSPS KQSPGESTKK QDGLVQIAPN LTVIQFIKVF 370 380 390 400 410 420 PRVLLQTLRH PIFLLVVLSQ VCLSSMAAGM AIFLPKFLER QFSITASYAN LLIGCLSFPS 430 440 450 460 470 480 VIVGIVVGGV LVKRLHLGPV GCGALCLLGM LLCLFFSLPL FFIGCSSHQI AGITHQTSAH 490 500 510 520 530 540 PGLELSPSCM EACSCPLDGF NPVCDPSTRV EYITPCHAGC SSWVVQDALD NSQVFYTNCS 550 560 570 580 590 600 CVVEGNPVLA GSCDSTCSHL VVPFLLLVSL GSALACLTHT PSFMLILRGV KKEDKTLAVG 610 620 630 640 650 660 IQFMFLRILA WMPSPVIHGS AIDTTCVHWA LSCGRRAVCR YYNNDLLRNR FIGLQFFFKT 670 680 690 700 GSVICFALVL AVLRQQDKEA RTKESRSSPA VEQQLLVSGP GKKPEDSRV
	Isoform 2 [UniParc]. Checksum: E436FE77E1355157	FASTA	482	52,489
	10 20 30 40 50 60 MIGYGAILVA LAGLLMTLPH FISEPYRYDN TSPGGISLTI KDPRWVGAWW LGFLIAAGAV 70 80 90 100 110 120 ALAAIPYFFF PKEMPKEKRE LQFRRKVLAV TDSPARKGKD SPSKQSPGES TKKQDGLVQI 130 140 150 160 170 180 APNLTVIQFI KVFPRVLLQT LRHPIFLLVV LSQVCLSSMA AGMAIFLPKF LERQFSITAS 190 200 210 220 230 240 YANLLIGCLS FPSVIVGIVV GGVLVKRLHL GPVGCGALCL LGMLLCLFFS LPLFFIGCSS 250 260 270 280 290 300 HQIAGITHQT SAHPGLELSP SCMEACSCPL DGFNPVCDPS TRVEYITPCH AGCSSWVVQD 310 320 330 340 350 360 ALDNSQVFYT NCSCVVEGNP VLAGSCDSTC SHLVVPFLLL VSLGSALACL THTPSFMLIL 370 380 390 400 410 420 RGVKKEDKTL AVGIQFMFLR ILAWMPSPVI HGSAIDTTCV HWALSCGRRA VCRYYNNDLL 430 440 450 460 470 480 RNRFIGLQFF FKTGSVICFA LVLAVLRQQD KEARTKESRS SPAVEQQLLV SGPGKKPEDS RV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification and characterization of novel members of the human organic anion transporter (OATP) family." Tamai I., Nezu J., Uchino H., Sai Y., Oku A., Shimane M., Tsuji A. Biochem. Biophys. Res. Commun. 273:251-260(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT THR-392. Tissue: Brain.
[2]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-392. Tissue: Brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-392. Tissue: Uterus.
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-392. Tissue: Brain.
[6]	"Identification and characterization of novel human OATP family members." Wu Y., Hsiang B.H., Zhu Y., Yang W.-P., Kirchgessner T.G. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-709 (ISOFORM 1), VARIANT THR-392.
[7]	"Functional analysis of the extracellular cysteine residues in the human organic anion transporting polypeptide, OATP2B1." Hanggi E., Grundschober A.F., Leuthold S., Meier P.J., St-Pierre M.V. Mol. Pharmacol. 70:806-817(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PRESENCE OF DISULFIDE BONDS.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-77.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026256 mRNA. Translation: BAA78638.1. AB020687 mRNA. Translation: BAA74903.2. Different initiation. AL117465 mRNA. Translation: CAB55940.1. AP001972 Genomic DNA. No translation available. BC041095 mRNA. Translation: AAH41095.1. AF205073 mRNA. Translation: AAG42205.1. Different initiation.
IPI	IPI00016553. IPI00220023.
PIR	T17250.
RefSeq	NP_001138683.1. NM_001145211.2. NP_001138684.1. NM_001145212.2. NP_009187.1. NM_007256.4.
UniGene	Hs.7884.
3D structure databases
ProteinModelPortal	O94956.
SMR	O94956. Positions 493-521.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000289575.
PTM databases
PhosphoSite	O94956.
Proteomic databases
PaxDb	O94956.
PRIDE	O94956.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000289575; ENSP00000289575; ENSG00000137491. ENST00000341411; ENSP00000341286; ENSG00000137491. ENST00000454962; ENSP00000389653; ENSG00000137491.
GeneID	11309.
KEGG	hsa:11309.
UCSC	uc001owb.3. human. uc001owc.3. human.
Organism-specific databases
CTD	11309.
GeneCards	GC11P074862.
HGNC	HGNC:10962. SLCO2B1.
HPA	HPA020659.
MIM	604988. gene.
neXtProt	NX_O94956.
PharmGKB	PA35845.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG318402.
HOGENOM	HOG000231269.
HOVERGEN	HBG108345.
InParanoid	O94956.
KO	K14352.
OMA	IQFIKVF.
OrthoDB	EOG46WZ88.
PhylomeDB	O94956.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	O94956.
Bgee	O94956.
CleanEx	HS_SLCO2B1.
Genevestigator	O94956.
GermOnline	ENSG00000137491. Homo sapiens.
Family and domain databases
InterPro	IPR002350. Kazal_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004156. OA_transporter. [Graphical view]
PANTHER	PTHR11388. PTHR11388. 1 hit.
Pfam	PF03137. OATP. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00805. oat. 1 hit.
PROSITE	PS51465. KAZAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1743124.
ChiTaRS	SLCO2B1. human.
DrugBank	DB01049. Ergoloid mesylate.
GenomeRNAi	11309.
NextBio	42967.
SOURCE	Search...

Entry information

Entry name

SO2B1_HUMAN

Accession

Primary (citable) accession number: O94956
Secondary accession number(s): Q9H2Z0, Q9UFU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.