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Protein

D-glucuronyl C5-epimerase

Gene

GLCE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.1 Publication

Catalytic activityi

Heparosan-N-sulfate D-glucuronate = heparosan-N-sulfate L-iduronate.2 Publications

Pathway:iheparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

Pathway:iheparin biosynthesis

This protein is involved in the pathway heparin biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparin biosynthesis and in Glycan metabolism.

GO - Molecular functioni

  • heparosan-N-sulfate-glucuronate 5-epimerase activity Source: UniProtKB
  • racemase and epimerase activity, acting on carbohydrates and derivatives Source: UniProtKB
  • UDP-glucuronate 5'-epimerase activity Source: HGNC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BRENDAi5.1.3.17. 2681.
ReactomeiREACT_121248. HS-GAG biosynthesis.
UniPathwayiUPA00756.
UPA00862.

Names & Taxonomyi

Protein namesi
Recommended name:
D-glucuronyl C5-epimerase (EC:5.1.3.17)
Alternative name(s):
Heparan sulfate C5-epimerase
Short name:
Hsepi
Heparin/heparan sulfate:glucuronic acid C5-epimerase
Heparosan-N-sulfate-glucuronate 5-epimerase
Gene namesi
Name:GLCE
Synonyms:KIAA0836Imported
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:17855. GLCE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei12 – 2817Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 617589LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi146 – 1461Y → A: Reduces enzyme activity by about 60%. 1 Publication
Mutagenesisi162 – 1621Y → A: Reduces enzyme activity by about 75%. 1 Publication
Mutagenesisi168 – 1681Y → A: Almost abolishes enzyme activity. 1 Publication
Mutagenesisi210 – 2101Y → A: Reduces enzyme activity by about 30%. 1 Publication
Mutagenesisi222 – 2221Y → A: Almost abolishes enzyme activity. 1 Publication

Organism-specific databases

PharmGKBiPA145148750.

Polymorphism and mutation databases

BioMutaiGLCE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 617617D-glucuronyl C5-epimerasePRO_0000192645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi225 – 2251N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO94923.
PaxDbiO94923.
PRIDEiO94923.

PTM databases

PhosphoSiteiO94923.

Expressioni

Gene expression databases

BgeeiO94923.
CleanExiHS_GLCE.
ExpressionAtlasiO94923. baseline and differential.
GenevisibleiO94923. HS.

Organism-specific databases

HPAiHPA040481.
HPA048216.

Interactioni

Subunit structurei

Interacts with HS2ST1.By similarity

Protein-protein interaction databases

BioGridi117501. 4 interactions.
IntActiO94923. 10 interactions.
STRINGi9606.ENSP00000261858.

Structurei

3D structure databases

ProteinModelPortaliO94923.
SMRiO94923. Positions 101-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the D-glucuronyl C5-epimerase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG86883.
GeneTreeiENSGT00390000006043.
HOGENOMiHOG000230542.
HOVERGENiHBG031576.
InParanoidiO94923.
KOiK01793.
OMAiWDYHATH.
PhylomeDBiO94923.
TreeFamiTF105869.

Family and domain databases

InterProiIPR010598. C5-epim.
[Graphical view]
PfamiPF06662. C5-epim_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCLAARVNY KTLIIICALF TLVTVLLWNK CSSDKAIQFP RRSSSGFRVD
60 70 80 90 100
GFEKRAAASE SNNYMNHVAK QQSEEAFPQE QQKAPPVVGG FNSNVGSKVL
110 120 130 140 150
GLKYEEIDCL INDEHTIKGR REGNEVFLPF TWVEKYFDVY GKVVQYDGYD
160 170 180 190 200
RFEFSHSYSK VYAQRAPYHP DGVFMSFEGY NVEVRDRVKC ISGVEGVPLS
210 220 230 240 250
TQWGPQGYFY PIQIAQYGLS HYSKNLTEKP PHIEVYETAE DRDKNKPNDW
260 270 280 290 300
TVPKGCFMAN VADKSRFTNV KQFIAPETSE GVSLQLGNTK DFIISFDLKF
310 320 330 340 350
LTNGSVSVVL ETTEKNQLFT IHYVSNAQLI AFKERDIYYG IGPRTSWSTV
360 370 380 390 400
TRDLVTDLRK GVGLSNTKAV KPTKIMPKKV VRLIAKGKGF LDNITISTTA
410 420 430 440 450
HMAAFFAASD WLVRNQDEKG GWPIMVTRKL GEGFKSLEPG WYSAMAQGQA
460 470 480 490 500
ISTLVRAYLL TKDHIFLNSA LRATAPYKFL SEQHGVKAVF MNKHDWYEEY
510 520 530 540 550
PTTPSSFVLN GFMYSLIGLY DLKETAGEKL GKEARSLYER GMESLKAMLP
560 570 580 590 600
LYDTGSGTIY DLRHFMLGIA PNLARWDYHT THINQLQLLS TIDESPVFKE
610
FVKRWKSYLK GSRAKHN
Length:617
Mass (Da):70,101
Last modified:June 16, 2009 - v3
Checksum:i2F909A15FEA6DA05
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti65 – 651M → V.
Corresponds to variant rs12440300 [ dbSNP | Ensembl ].
VAR_055837
Natural varianti597 – 5971V → I.1 Publication
Corresponds to variant rs3865014 [ dbSNP | Ensembl ].
VAR_057958

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY635582 mRNA. Translation: AAT48654.1.
AC026992 mRNA. No translation available.
AB020643 mRNA. Translation: BAA74859.1.
CCDSiCCDS32277.1.
RefSeqiNP_056369.1. NM_015554.1.
XP_005254355.1. XM_005254298.2.
UniGeneiHs.183006.

Genome annotation databases

EnsembliENST00000261858; ENSP00000261858; ENSG00000138604.
GeneIDi26035.
KEGGihsa:26035.
UCSCiuc002ary.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY635582 mRNA. Translation: AAT48654.1.
AC026992 mRNA. No translation available.
AB020643 mRNA. Translation: BAA74859.1.
CCDSiCCDS32277.1.
RefSeqiNP_056369.1. NM_015554.1.
XP_005254355.1. XM_005254298.2.
UniGeneiHs.183006.

3D structure databases

ProteinModelPortaliO94923.
SMRiO94923. Positions 101-617.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117501. 4 interactions.
IntActiO94923. 10 interactions.
STRINGi9606.ENSP00000261858.

PTM databases

PhosphoSiteiO94923.

Polymorphism and mutation databases

BioMutaiGLCE.

Proteomic databases

MaxQBiO94923.
PaxDbiO94923.
PRIDEiO94923.

Protocols and materials databases

DNASUi26035.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261858; ENSP00000261858; ENSG00000138604.
GeneIDi26035.
KEGGihsa:26035.
UCSCiuc002ary.1. human.

Organism-specific databases

CTDi26035.
GeneCardsiGC15P069452.
HGNCiHGNC:17855. GLCE.
HPAiHPA040481.
HPA048216.
MIMi612134. gene.
neXtProtiNX_O94923.
PharmGKBiPA145148750.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG86883.
GeneTreeiENSGT00390000006043.
HOGENOMiHOG000230542.
HOVERGENiHBG031576.
InParanoidiO94923.
KOiK01793.
OMAiWDYHATH.
PhylomeDBiO94923.
TreeFamiTF105869.

Enzyme and pathway databases

UniPathwayiUPA00756.
UPA00862.
BRENDAi5.1.3.17. 2681.
ReactomeiREACT_121248. HS-GAG biosynthesis.

Miscellaneous databases

GeneWikiiGLCE.
GenomeRNAii26035.
NextBioi47849.
PROiO94923.
SOURCEiSearch...

Gene expression databases

BgeeiO94923.
CleanExiHS_GLCE.
ExpressionAtlasiO94923. baseline and differential.
GenevisibleiO94923. HS.

Family and domain databases

InterProiIPR010598. C5-epim.
[Graphical view]
PfamiPF06662. C5-epim_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homo sapiens D-glucuronyl C5-epimerase (GLCE)."
    Ghiselli G.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-617, VARIANT ILE-597.
    Tissue: Brain.
  4. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225.
    Tissue: Liver.
  5. "Using engineered 2-O-sulfotransferase to determine the activity of heparan sulfate C5-epimerase and its mutants."
    Li K., Bethea H.N., Liu J.
    J. Biol. Chem. 285:11106-11113(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, MUTAGENESIS OF TYR-146; TYR-162; TYR-168; TYR-210 AND TYR-222.
  6. "Uncovering a biphasic catalytic mode of C5-epimerase in heparan sulfate biosynthesis."
    Sheng J., Xu Y., Dulaney S.B., Huang X., Liu J.
    J. Biol. Chem. 287:20996-21002(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.

Entry informationi

Entry nameiGLCE_HUMAN
AccessioniPrimary (citable) accession number: O94923
Secondary accession number(s): Q6GUQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: June 16, 2009
Last modified: July 22, 2015
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.