O94916 (NFAT5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear factor of activated T-cells 5
Short name=NF-AT5

Alternative name(s):
T-cell transcription factor NFAT5
Tonicity-responsive enhancer-binding protein
Short name=TonE-binding protein
Short name=TonEBP

Gene names

Name:	NFAT5
Synonyms:	KIAA0827, TONEBP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1531 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.
Subunit structure	Homodimer when bound to DNA, completely encircles its DNA target. Does not bind with Fos and Jun transcription factors. Ref.10
Subcellular location	Nucleus.
Tissue specificity	Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Post-translational modification	Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization. Ref.11
Sequence similarities	Contains 1 RHD (Rel-like) domain.
Sequence caution	The sequence BAA74850.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAB09693.1 differs from that shown. Reason: Frameshift at position 1164.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	excretion Traceable author statement Ref.3. Source: ProtInc signal transduction Non-traceable author statement Ref.2. Source: ProtInc transcription from RNA polymerase II promoter Traceable author statement Ref.3. Source: ProtInc
Cellular_component	nucleus Traceable author statement Ref.3. Source: ProtInc
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement Ref.3. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DDX17	Q92841	3	EBI-308320,EBI-746012
DDX5	P17844	4	EBI-308320,EBI-351962

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform C (identifier: O94916-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: O94916-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Isoform B (identifier: O94916-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Isoform D (identifier: O94916-4)

The sequence of this isoform differs from the canonical sequence as follows:
     24-24: R → RDSLKLHPSQNFHRAGLLE
     546-546: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1531

1531

Nuclear factor of activated T-cells 5

PRO_0000205183

Regions

Domain

264 – 443

180

RHD

DNA binding

293 – 300

8

Compositional bias

69 – 100

32

Ser-rich

Compositional bias

739 – 743

5

Poly-Gln

Compositional bias

879 – 888

10

Poly-Gln

Compositional bias

966 – 971

6

Poly-Thr

Compositional bias

1248 – 1266

19

Poly-Gln

Amino acid modifications

Modified residue

120

1

Phosphoserine Ref.11

Modified residue

134

1

Phosphoserine Ref.11

Modified residue

135

1

Phosphothreonine; by CDK5 Ref.11

Modified residue

155

1

Phosphoserine Ref.11

Natural variations

Alternative sequence

1 – 76

76

Missing in isoform A.

VSP_005605

Alternative sequence

1 – 47

47

Missing in isoform B.

VSP_005606

Alternative sequence

24

1

R → RDSLKLHPSQNFHRAGLLE in isoform D.

VSP_043840

Alternative sequence

546

1

Missing in isoform D.

VSP_043841

Experimental info

Mutagenesis

120

1

S → A: Normal nuclear localization. Ref.11

Mutagenesis

134

1

S → A: Reduced nuclear localization. Ref.11

Mutagenesis

135

1

T → A: Reduced nuclear localization. Ref.11

Mutagenesis

155

1

S → A: Increased nuclear localization. Ref.11

Sequence conflict

1369

1

E → D in CAB09693. Ref.8

Secondary structure

1

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1531

Helix Strand Turn

Details...

Beta strand

275 – 277

3

Beta strand

280 – 286

7

Beta strand

310 – 312

3

Beta strand

315 – 320

6

Beta strand

325 – 337

13

Beta strand

342 – 348

7

Beta strand

358 – 362

5

Beta strand

365 – 372

8

Helix

374 – 376

3

Beta strand

379 – 381

3

Beta strand

384 – 389

6

Helix

392 – 396

5

Beta strand

410 – 420

11

Beta strand

422 – 424

3

Beta strand

426 – 432

7

Beta strand

446 – 451

6

Beta strand

454 – 456

3

Beta strand

462 – 468

7

Beta strand

475 – 482

8

Beta strand

489 – 492

4

Helix

496 – 498

3

Beta strand

501 – 507

7

Beta strand

520 – 528

9

Beta strand

537 – 542

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform C [UniParc]. Last modified May 1, 1999. Version 1. Checksum: A68C68088DABF69E	FASTA	1,531	165,763
	10 20 30 40 50 60 MPSDFISLLS ADLDLESPKS LYSRESVYDL LPKELQLPPS RETSVASMSQ TSGGEAGSPP 70 80 90 100 110 120 PAVVAADASS APSSSSMGGA CSSFTTSSSP TIYSTSVTDS KAMQVESCSS AVGVSNRGVS 130 140 150 160 170 180 EKQLTSNTVQ QHPSTPKRHT VLYISPPPED LLDNSRMSCQ DEGCGLESEQ SCSMWMEDSP 190 200 210 220 230 240 SNFSNMSTSS YNDNTEVPRK SRKRNPKQRP GVKRRDCEES NMDIFDADSA KAPHYVLSQL 250 260 270 280 290 300 TTDNKGNSKA GNGTLENQKG TGVKKSPMLC GQYPVKSEGK ELKIVVQPET QHRARYLTEG 310 320 330 340 350 360 SRGSVKDRTQ QGFPTVKLEG HNEPVVLQVF VGNDSGRVKP HGFYQACRVT GRNTTPCKEV 370 380 390 400 410 420 DIEGTTVIEV GLDPSNNMTL AVDCVGILKL RNADVEARIG IAGSKKKSTR ARLVFRVNIM 430 440 450 460 470 480 RKDGSTLTLQ TPSSPILCTQ PAGVPEILKK SLHSCSVKGE EEVFLIGKNF LKGTKVIFQE 490 500 510 520 530 540 NVSDENSWKS EAEIDMELFH QNHLIVKVPP YHDQHITLPV SVGIYVVTNA GRSHDVQPFT 550 560 570 580 590 600 YTPDPAAAGA LNVNVKKEIS SPARPCSFEE AMKAMKTTGC NLDKVNIIPN ALMTPLIPSS 610 620 630 640 650 660 MIKSEDVTPM EVTAEKRSST IFKTTKSVGS TQQTLENISN IAGNGSFSSP SSSHLPSENE 670 680 690 700 710 720 KQQQIQPKAY NPETLTTIQT QDISQPGTFP AVSASSQLPN SDALLQQATQ FQTRETQSRE 730 740 750 760 770 780 ILQSDGTVVN LSQLTEASQQ QQQSPLQEQA QTLQQQISSN IFPSPNSVSQ LQNTIQQLQA 790 800 810 820 830 840 GSFTGSTASG SSGSVDLVQQ VLEAQQQLSS VLFSAPDGNE NVQEQLSADI FQQVSQIQSG 850 860 870 880 890 900 VSPGMFSSTE PTVHTRPDNL LPGRAESVHP QSENTLSNQQ QQQQQQQQVM ESSAAMVMEM 910 920 930 940 950 960 QQSICQAAAQ IQSELFPSTA SANGNLQQSP VYQQTSHMMS ALSTNEDMQM QCELFSSPPA 970 980 990 1000 1010 1020 VSGNETSTTT TQQVATPGTT MFQTSSSGDG EETGTQAKQI QNSVFQTMVQ MQHSGDNQPQ 1030 1040 1050 1060 1070 1080 VNLFSSTKSM MSVQNSGTQQ QGNGLFQQGN EMMSLQSGNF LQQSSHSQAQ LFHPQNPIAD 1090 1100 1110 1120 1130 1140 AQNLSQETQG SLFHSPNPIV HSQTSTTSSE QMQPPMFHSQ STIAVLQGSS VPQDQQSTNI 1150 1160 1170 1180 1190 1200 FLSQSPMNNL QTNTVAQEAF FAAPNSISPL QSTSNSEQQA AFQQQAPISH IQTPMLSQEQ 1210 1220 1230 1240 1250 1260 AQPPQQGLFQ PQVALGSLPP NPMPQSQQGT MFQSQHSIVA MQSNSPSQEQ QQQQQQQQQQ 1270 1280 1290 1300 1310 1320 QQQQQQSILF SNQNTMATMA SPKQPPPNMI FNPNQNPMAN QEQQNQSIFH QQSNMAPMNQ 1330 1340 1350 1360 1370 1380 EQQPMQFQSQ STVSSLQNPG PTQSESSQTP LFHSSPQIQL VQGSPSSQEQ QVTLFLSPAS 1390 1400 1410 1420 1430 1440 MSALQTSINQ QDMQQSPLYS PQNNMPGIQG ATSSPQPQAT LFHNTAGGTM NQLQNSPGSS 1450 1460 1470 1480 1490 1500 QQTSGMFLFG IQNNCSQLLT SGPATLPDQL MAISQPGQPQ NEGQPPVTTL LSQQMPENSP 1510 1520 1530 LASSINTNQN IEKIDLLVSL QNQGNNLTGS F
	Isoform A [UniParc]. Checksum: 4FA637348EAC0041	FASTA	1,455	157,983
	10 20 30 40 50 60 MGGACSSFTT SSSPTIYSTS VTDSKAMQVE SCSSAVGVSN RGVSEKQLTS NTVQQHPSTP 70 80 90 100 110 120 KRHTVLYISP PPEDLLDNSR MSCQDEGCGL ESEQSCSMWM EDSPSNFSNM STSSYNDNTE 130 140 150 160 170 180 VPRKSRKRNP KQRPGVKRRD CEESNMDIFD ADSAKAPHYV LSQLTTDNKG NSKAGNGTLE 190 200 210 220 230 240 NQKGTGVKKS PMLCGQYPVK SEGKELKIVV QPETQHRARY LTEGSRGSVK DRTQQGFPTV 250 260 270 280 290 300 KLEGHNEPVV LQVFVGNDSG RVKPHGFYQA CRVTGRNTTP CKEVDIEGTT VIEVGLDPSN 310 320 330 340 350 360 NMTLAVDCVG ILKLRNADVE ARIGIAGSKK KSTRARLVFR VNIMRKDGST LTLQTPSSPI 370 380 390 400 410 420 LCTQPAGVPE ILKKSLHSCS VKGEEEVFLI GKNFLKGTKV IFQENVSDEN SWKSEAEIDM 430 440 450 460 470 480 ELFHQNHLIV KVPPYHDQHI TLPVSVGIYV VTNAGRSHDV QPFTYTPDPA AAGALNVNVK 490 500 510 520 530 540 KEISSPARPC SFEEAMKAMK TTGCNLDKVN IIPNALMTPL IPSSMIKSED VTPMEVTAEK 550 560 570 580 590 600 RSSTIFKTTK SVGSTQQTLE NISNIAGNGS FSSPSSSHLP SENEKQQQIQ PKAYNPETLT 610 620 630 640 650 660 TIQTQDISQP GTFPAVSASS QLPNSDALLQ QATQFQTRET QSREILQSDG TVVNLSQLTE 670 680 690 700 710 720 ASQQQQQSPL QEQAQTLQQQ ISSNIFPSPN SVSQLQNTIQ QLQAGSFTGS TASGSSGSVD 730 740 750 760 770 780 LVQQVLEAQQ QLSSVLFSAP DGNENVQEQL SADIFQQVSQ IQSGVSPGMF SSTEPTVHTR 790 800 810 820 830 840 PDNLLPGRAE SVHPQSENTL SNQQQQQQQQ QQVMESSAAM VMEMQQSICQ AAAQIQSELF 850 860 870 880 890 900 PSTASANGNL QQSPVYQQTS HMMSALSTNE DMQMQCELFS SPPAVSGNET STTTTQQVAT 910 920 930 940 950 960 PGTTMFQTSS SGDGEETGTQ AKQIQNSVFQ TMVQMQHSGD NQPQVNLFSS TKSMMSVQNS 970 980 990 1000 1010 1020 GTQQQGNGLF QQGNEMMSLQ SGNFLQQSSH SQAQLFHPQN PIADAQNLSQ ETQGSLFHSP 1030 1040 1050 1060 1070 1080 NPIVHSQTST TSSEQMQPPM FHSQSTIAVL QGSSVPQDQQ STNIFLSQSP MNNLQTNTVA 1090 1100 1110 1120 1130 1140 QEAFFAAPNS ISPLQSTSNS EQQAAFQQQA PISHIQTPML SQEQAQPPQQ GLFQPQVALG 1150 1160 1170 1180 1190 1200 SLPPNPMPQS QQGTMFQSQH SIVAMQSNSP SQEQQQQQQQ QQQQQQQQQQ SILFSNQNTM 1210 1220 1230 1240 1250 1260 ATMASPKQPP PNMIFNPNQN PMANQEQQNQ SIFHQQSNMA PMNQEQQPMQ FQSQSTVSSL 1270 1280 1290 1300 1310 1320 QNPGPTQSES SQTPLFHSSP QIQLVQGSPS SQEQQVTLFL SPASMSALQT SINQQDMQQS 1330 1340 1350 1360 1370 1380 PLYSPQNNMP GIQGATSSPQ PQATLFHNTA GGTMNQLQNS PGSSQQTSGM FLFGIQNNCS 1390 1400 1410 1420 1430 1440 QLLTSGPATL PDQLMAISQP GQPQNEGQPP VTTLLSQQMP ENSPLASSIN TNQNIEKIDL 1450 LVSLQNQGNN LTGSF
	Isoform B [UniParc]. Checksum: F3E61D1947D310D3	FASTA	1,484	160,556
	10 20 30 40 50 60 MSQTSGGEAG SPPPAVVAAD ASSAPSSSSM GGACSSFTTS SSPTIYSTSV TDSKAMQVES 70 80 90 100 110 120 CSSAVGVSNR GVSEKQLTSN TVQQHPSTPK RHTVLYISPP PEDLLDNSRM SCQDEGCGLE 130 140 150 160 170 180 SEQSCSMWME DSPSNFSNMS TSSYNDNTEV PRKSRKRNPK QRPGVKRRDC EESNMDIFDA 190 200 210 220 230 240 DSAKAPHYVL SQLTTDNKGN SKAGNGTLEN QKGTGVKKSP MLCGQYPVKS EGKELKIVVQ 250 260 270 280 290 300 PETQHRARYL TEGSRGSVKD RTQQGFPTVK LEGHNEPVVL QVFVGNDSGR VKPHGFYQAC 310 320 330 340 350 360 RVTGRNTTPC KEVDIEGTTV IEVGLDPSNN MTLAVDCVGI LKLRNADVEA RIGIAGSKKK 370 380 390 400 410 420 STRARLVFRV NIMRKDGSTL TLQTPSSPIL CTQPAGVPEI LKKSLHSCSV KGEEEVFLIG 430 440 450 460 470 480 KNFLKGTKVI FQENVSDENS WKSEAEIDME LFHQNHLIVK VPPYHDQHIT LPVSVGIYVV 490 500 510 520 530 540 TNAGRSHDVQ PFTYTPDPAA AGALNVNVKK EISSPARPCS FEEAMKAMKT TGCNLDKVNI 550 560 570 580 590 600 IPNALMTPLI PSSMIKSEDV TPMEVTAEKR SSTIFKTTKS VGSTQQTLEN ISNIAGNGSF 610 620 630 640 650 660 SSPSSSHLPS ENEKQQQIQP KAYNPETLTT IQTQDISQPG TFPAVSASSQ LPNSDALLQQ 670 680 690 700 710 720 ATQFQTRETQ SREILQSDGT VVNLSQLTEA SQQQQQSPLQ EQAQTLQQQI SSNIFPSPNS 730 740 750 760 770 780 VSQLQNTIQQ LQAGSFTGST ASGSSGSVDL VQQVLEAQQQ LSSVLFSAPD GNENVQEQLS 790 800 810 820 830 840 ADIFQQVSQI QSGVSPGMFS STEPTVHTRP DNLLPGRAES VHPQSENTLS NQQQQQQQQQ 850 860 870 880 890 900 QVMESSAAMV MEMQQSICQA AAQIQSELFP STASANGNLQ QSPVYQQTSH MMSALSTNED 910 920 930 940 950 960 MQMQCELFSS PPAVSGNETS TTTTQQVATP GTTMFQTSSS GDGEETGTQA KQIQNSVFQT 970 980 990 1000 1010 1020 MVQMQHSGDN QPQVNLFSST KSMMSVQNSG TQQQGNGLFQ QGNEMMSLQS GNFLQQSSHS 1030 1040 1050 1060 1070 1080 QAQLFHPQNP IADAQNLSQE TQGSLFHSPN PIVHSQTSTT SSEQMQPPMF HSQSTIAVLQ 1090 1100 1110 1120 1130 1140 GSSVPQDQQS TNIFLSQSPM NNLQTNTVAQ EAFFAAPNSI SPLQSTSNSE QQAAFQQQAP 1150 1160 1170 1180 1190 1200 ISHIQTPMLS QEQAQPPQQG LFQPQVALGS LPPNPMPQSQ QGTMFQSQHS IVAMQSNSPS 1210 1220 1230 1240 1250 1260 QEQQQQQQQQ QQQQQQQQQS ILFSNQNTMA TMASPKQPPP NMIFNPNQNP MANQEQQNQS 1270 1280 1290 1300 1310 1320 IFHQQSNMAP MNQEQQPMQF QSQSTVSSLQ NPGPTQSESS QTPLFHSSPQ IQLVQGSPSS 1330 1340 1350 1360 1370 1380 QEQQVTLFLS PASMSALQTS INQQDMQQSP LYSPQNNMPG IQGATSSPQP QATLFHNTAG 1390 1400 1410 1420 1430 1440 GTMNQLQNSP GSSQQTSGMF LFGIQNNCSQ LLTSGPATLP DQLMAISQPG QPQNEGQPPV 1450 1460 1470 1480 TTLLSQQMPE NSPLASSINT NQNIEKIDLL VSLQNQGNNL TGSF
	Isoform D [UniParc]. Checksum: 48E5072E004F9C09	FASTA	1,548	167,737
	10 20 30 40 50 60 MPSDFISLLS ADLDLESPKS LYSRDSLKLH PSQNFHRAGL LEESVYDLLP KELQLPPSRE 70 80 90 100 110 120 TSVASMSQTS GGEAGSPPPA VVAADASSAP SSSSMGGACS SFTTSSSPTI YSTSVTDSKA 130 140 150 160 170 180 MQVESCSSAV GVSNRGVSEK QLTSNTVQQH PSTPKRHTVL YISPPPEDLL DNSRMSCQDE 190 200 210 220 230 240 GCGLESEQSC SMWMEDSPSN FSNMSTSSYN DNTEVPRKSR KRNPKQRPGV KRRDCEESNM 250 260 270 280 290 300 DIFDADSAKA PHYVLSQLTT DNKGNSKAGN GTLENQKGTG VKKSPMLCGQ YPVKSEGKEL 310 320 330 340 350 360 KIVVQPETQH RARYLTEGSR GSVKDRTQQG FPTVKLEGHN EPVVLQVFVG NDSGRVKPHG 370 380 390 400 410 420 FYQACRVTGR NTTPCKEVDI EGTTVIEVGL DPSNNMTLAV DCVGILKLRN ADVEARIGIA 430 440 450 460 470 480 GSKKKSTRAR LVFRVNIMRK DGSTLTLQTP SSPILCTQPA GVPEILKKSL HSCSVKGEEE 490 500 510 520 530 540 VFLIGKNFLK GTKVIFQENV SDENSWKSEA EIDMELFHQN HLIVKVPPYH DQHITLPVSV 550 560 570 580 590 600 GIYVVTNAGR SHDVQPFTYT PDPAAGALNV NVKKEISSPA RPCSFEEAMK AMKTTGCNLD 610 620 630 640 650 660 KVNIIPNALM TPLIPSSMIK SEDVTPMEVT AEKRSSTIFK TTKSVGSTQQ TLENISNIAG 670 680 690 700 710 720 NGSFSSPSSS HLPSENEKQQ QIQPKAYNPE TLTTIQTQDI SQPGTFPAVS ASSQLPNSDA 730 740 750 760 770 780 LLQQATQFQT RETQSREILQ SDGTVVNLSQ LTEASQQQQQ SPLQEQAQTL QQQISSNIFP 790 800 810 820 830 840 SPNSVSQLQN TIQQLQAGSF TGSTASGSSG SVDLVQQVLE AQQQLSSVLF SAPDGNENVQ 850 860 870 880 890 900 EQLSADIFQQ VSQIQSGVSP GMFSSTEPTV HTRPDNLLPG RAESVHPQSE NTLSNQQQQQ 910 920 930 940 950 960 QQQQQVMESS AAMVMEMQQS ICQAAAQIQS ELFPSTASAN GNLQQSPVYQ QTSHMMSALS 970 980 990 1000 1010 1020 TNEDMQMQCE LFSSPPAVSG NETSTTTTQQ VATPGTTMFQ TSSSGDGEET GTQAKQIQNS 1030 1040 1050 1060 1070 1080 VFQTMVQMQH SGDNQPQVNL FSSTKSMMSV QNSGTQQQGN GLFQQGNEMM SLQSGNFLQQ 1090 1100 1110 1120 1130 1140 SSHSQAQLFH PQNPIADAQN LSQETQGSLF HSPNPIVHSQ TSTTSSEQMQ PPMFHSQSTI 1150 1160 1170 1180 1190 1200 AVLQGSSVPQ DQQSTNIFLS QSPMNNLQTN TVAQEAFFAA PNSISPLQST SNSEQQAAFQ 1210 1220 1230 1240 1250 1260 QQAPISHIQT PMLSQEQAQP PQQGLFQPQV ALGSLPPNPM PQSQQGTMFQ SQHSIVAMQS 1270 1280 1290 1300 1310 1320 NSPSQEQQQQ QQQQQQQQQQ QQQSILFSNQ NTMATMASPK QPPPNMIFNP NQNPMANQEQ 1330 1340 1350 1360 1370 1380 QNQSIFHQQS NMAPMNQEQQ PMQFQSQSTV SSLQNPGPTQ SESSQTPLFH SSPQIQLVQG 1390 1400 1410 1420 1430 1440 SPSSQEQQVT LFLSPASMSA LQTSINQQDM QQSPLYSPQN NMPGIQGATS SPQPQATLFH 1450 1460 1470 1480 1490 1500 NTAGGTMNQL QNSPGSSQQT SGMFLFGIQN NCSQLLTSGP ATLPDQLMAI SQPGQPQNEG 1510 1520 1530 1540 QPPVTTLLSQ QMPENSPLAS SINTNQNIEK IDLLVSLQNQ GNNLTGSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Tissue: Brain.
[2]	"NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun." Lopez-Rodriguez C., Aramburu J., Rakeman A.S., Rao A. Proc. Natl. Acad. Sci. U.S.A. 96:7214-7219(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Brain.
[3]	"Tonicity-responsive enhancer binding protein, a rel-like protein that stimulates transcription in response to hypertonicity." Miyakawa H., Woo S.K., Dahl S.C., Handler J.S., Kwon H.M. Proc. Natl. Acad. Sci. U.S.A. 96:2538-2542(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[4]	"NFAT5: the NF-AT family of transcription factors expands in a new direction." Lopez-Rodriguez C., Aramburu J., Rakeman A.S., Copeland N.G., Gilbert D.J., Thomas S., Disteche C., Jenkins N.A., Rao A. Cold Spring Harb. Symp. Quant. Biol. 64:517-526(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Brain.
[5]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS C AND D).
[8]	"Isolation and characterization of novel CAG repeat containing genes expressed in human brain." Zuehlke C., Kiehl R., Johannsmeyer A., Grzeschik K.H., Schwinger E. DNA Seq. 10:1-6(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 675-1531. Tissue: Brain.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Structure of a TonEBP-DNA complex reveals DNA encircled by a transcription factor." Stroud J.C., Lopez-Rodriguez C., Rao A., Chen L. Nat. Struct. Biol. 9:90-94(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.86 ANGSTROMS) OF 264-544 IN COMPLEX WITH DNA, SUBUNIT.
[11]	"High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization." Gallazzini M., Heussler G.E., Kunin M., Izumi Y., Burg M.B., Ferraris J.D. Mol. Biol. Cell 22:703-714(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-120; SER-134; THR-135 AND SER-155, MUTAGENESIS OF SER-120; SER-134; THR-135 AND SER-155.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB020634 mRNA. Translation: BAA74850.2. Different initiation.
AF134870 mRNA. Translation: AAD38360.1.
AF089824 mRNA. Translation: AAD18136.1.
AF163836 mRNA. Translation: AAD48441.1.
AC009032 Genomic DNA. No translation available.
AC012321 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83281.1.
BC131509 mRNA. Translation: AAI31510.1.
BC146765 mRNA. Translation: AAI46766.1.
Z97016 mRNA. Translation: CAB09693.1. Frameshift.

IPI

IPI00217710.
IPI00218771.
IPI00218772.

RefSeq

NP_001106649.1. NM_001113178.2.
NP_006590.1. NM_006599.3.
NP_619727.2. NM_138713.3.
NP_619728.2. NM_138714.3.
NP_775321.1. NM_173214.2.
NP_775322.1. NM_173215.2.

UniGene

Hs.371987.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IMH	X-ray	2.86	C/D	264-544	[»]

ProteinModelPortal

O94916.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-58524N.

IntAct

O94916. 5 interactions.

STRING

9606.ENSP00000396538.

PTM databases

PhosphoSite

O94916.

Proteomic databases

PaxDb

O94916.

PRIDE

O94916.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000349945; ENSP00000338806; ENSG00000102908.
ENST00000354436; ENSP00000346420; ENSG00000102908.
ENST00000393742; ENSP00000377343; ENSG00000102908.
ENST00000566899; ENSP00000455628; ENSG00000102908.
ENST00000567239; ENSP00000457593; ENSG00000102908.

GeneID

10725.

KEGG

hsa:10725.

UCSC

uc002exi.3. human.

Organism-specific databases

CTD

10725.

GeneCards

GC16P069599.

HGNC

HGNC:7774. NFAT5.

MIM

604708. gene.

neXtProt

NX_O94916.

PharmGKB

PA31581.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG85658.

HOGENOM

HOG000236245.

HOVERGEN

HBG052612.

InParanoid

O94916.

KO

K04446.

OrthoDB

EOG48GW2P.

Gene expression databases

ArrayExpress

O94916.

Bgee

O94916.

CleanEx

HS_NFAT5.

Genevestigator

O94916.

GermOnline

ENSG00000102908. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
2.60.40.340. 1 hit.

InterPro

IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT_TIG_rcpt.
IPR008366. NFAT.
IPR015646. NFAT5.
IPR008967. p53-like_TF_DNA-bd.
IPR011539. RHD.
[Graphical view]

PANTHER

PTHR12533. PTHR12533. 1 hit.
PTHR12533:SF1. PTHR12533:SF1. 1 hit.

Pfam

PF00554. RHD. 1 hit.
[Graphical view]

PRINTS

PR01789. NUCFACTORATC.

SMART

SM00429. IPT. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.
SSF49417. P53_like_DNA_bnd. 1 hit.

PROSITE

PS01204. REL_1. False negative.
PS50254. REL_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

NFAT5. human.

EvolutionaryTrace

O94916.

GenomeRNAi

10725.

NextBio

40713.

SOURCE

Search...

Entry information

Entry name

NFAT5_HUMAN

Accession

Primary (citable) accession number: O94916
Secondary accession number(s): A2RRB4 , A6H8V5, O95693, Q9UN18

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 135 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.