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O94910 (LPHN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Latrophilin-1
Alternative name(s):
Calcium-independent alpha-latrotoxin receptor 1
Short name=CIRL-1
Lectomedin-2
Gene names
Name:LPHN1
Synonyms:KIAA0821, LEC2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization. Receptor propably implicated in the regulation of exocytosis By similarity.

Subunit structure

Forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). Interacts with syntaxin and with proteins of the SHANK family via the PDZ domain By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell projectionaxon By similarity. Cell projectiongrowth cone By similarity. Cell junctionsynapse By similarity. Cell junctionsynapsepresynaptic cell membrane By similarity. Cell junctionsynapsesynaptosome By similarity. Note: Colocalizes with TENM2 on the cell surface, across intercellular junctions and on nerve terminals near synaptic clefts By similarity.

Domain

The extracellular domain coupled to the a single transmembrane region are sufficient for full responsiveness to alpha-latrotoxin By similarity.

Post-translational modification

Autoproteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. This proteolytic processing takes place early in the biosynthetic pathway, either in the endoplasmic reticulum or in the early compartment of the Golgi apparatus By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Contains 1 GPS domain.

Contains 1 olfactomedin-like domain.

Contains 1 SUEL-type lectin domain.

Sequence caution

The sequence BAA74844.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Cell projection
Membrane
Synapse
Synaptosome
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   LigandLectin
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 15203201. Source: GDB

calcium-mediated signaling using intracellular calcium source

Inferred from sequence or structural similarity. Source: UniProtKB

heterophilic cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

neuropeptide signaling pathway

Inferred from electronic annotation. Source: InterPro

positive regulation of synapse maturation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentaxon

Inferred from sequence or structural similarity. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

growth cone

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Traceable author statement PubMed 15203201. Source: GDB

neuron projection

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

presynaptic membrane

Inferred from sequence or structural similarity. Source: UniProtKB

synapse

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionG-protein coupled receptor activity

Traceable author statement PubMed 15203201. Source: GDB

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

cell adhesion molecule binding

Inferred from sequence or structural similarity. Source: UniProtKB

latrotoxin receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CACNA1AO005552EBI-3389315,EBI-766279

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O94910-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O94910-2)

The sequence of this isoform differs from the canonical sequence as follows:
     132-137: KVEQKV → I

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 By similarity
Chain25 – 14741450Latrophilin-1
PRO_0000012907

Regions

Topological domain25 – 858834Extracellular Potential
Transmembrane859 – 87921Helical; Name=1; Potential
Topological domain880 – 89314Cytoplasmic Potential
Transmembrane894 – 91421Helical; Name=2; Potential
Topological domain915 – 9206Extracellular Potential
Transmembrane921 – 94121Helical; Name=3; Potential
Topological domain942 – 96423Cytoplasmic Potential
Transmembrane965 – 98521Helical; Name=4; Potential
Topological domain986 – 100217Extracellular Potential
Transmembrane1003 – 102321Helical; Name=5; Potential
Topological domain1024 – 105027Cytoplasmic Potential
Transmembrane1051 – 107121Helical; Name=6; Potential
Topological domain1072 – 10754Extracellular Potential
Transmembrane1076 – 109621Helical; Name=7; Potential
Topological domain1097 – 1474378Cytoplasmic Potential
Domain40 – 12990SUEL-type lectin
Domain139 – 398260Olfactomedin-like
Domain799 – 85052GPS
Region117 – 1204Carbohydrate binding By similarity
Compositional bias413 – 4164Poly-Thr
Compositional bias1303 – 131412Poly-Pro
Compositional bias1315 – 13184Poly-Gly
Compositional bias1410 – 142011Poly-Pro

Sites

Binding site421Carbohydrate By similarity
Site838 – 8392Cleavage; by autocatalysis By similarity

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation5311N-linked (GlcNAc...) Potential
Glycosylation6401N-linked (GlcNAc...) Potential
Glycosylation7421N-linked (GlcNAc...) Potential
Glycosylation8011N-linked (GlcNAc...) Potential
Glycosylation8061N-linked (GlcNAc...) Potential
Glycosylation8271N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 71 By similarity
Disulfide bond50 ↔ 128 By similarity
Disulfide bond83 ↔ 115 By similarity
Disulfide bond96 ↔ 102 By similarity
Disulfide bond140 ↔ 322 By similarity
Disulfide bond480 ↔ 515 By similarity
Disulfide bond503 ↔ 532 By similarity
Disulfide bond802 ↔ 833 By similarity
Disulfide bond821 ↔ 835 By similarity

Natural variations

Alternative sequence132 – 1376KVEQKV → I in isoform 2.
VSP_010099
Natural variant5951E → Q.
Corresponds to variant rs34759320 [ dbSNP | Ensembl ].
VAR_049463

Experimental info

Sequence conflict13211E → V in AAH02974. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 6152EB2DE1385F5C

FASTA1,474162,717
        10         20         30         40         50         60 
MARLAAVLWN LCVTAVLVTS ATQGLSRAGL PFGLMRRELA CEGYPIELRC PGSDVIMVEN 

        70         80         90        100        110        120 
ANYGRTDDKI CDADPFQMEN VQCYLPDAFK IMSQRCNNRT QCVVVAGSDA FPDPCPGTYK 

       130        140        150        160        170        180 
YLEVQYDCVP YKVEQKVFVC PGTLQKVLEP TSTHESEHQS GAWCKDPLQA GDRIYVMPWI 

       190        200        210        220        230        240 
PYRTDTLTEY ASWEDYVAAR HTTTYRLPNR VDGTGFVVYD GAVFYNKERT RNIVKYDLRT 

       250        260        270        280        290        300 
RIKSGETVIN TANYHDTSPY RWGGKTDIDL AVDENGLWVI YATEGNNGRL VVSQLNPYTL 

       310        320        330        340        350        360 
RFEGTWETGY DKRSASNAFM VCGVLYVLRS VYVDDDSEAA GNRVDYAFNT NANREEPVSL 

       370        380        390        400        410        420 
TFPNPYQFIS SVDYNPRDNQ LYVWNNYFVV RYSLEFGPPD PSAGPATSPP LSTTTTARPT 

       430        440        450        460        470        480 
PLTSTASPAA TTPLRRAPLT THPVGAINQL GPDLPPATAP VPSTRRPPAP NLHVSPELFC 

       490        500        510        520        530        540 
EPREVRRVQW PATQQGMLVE RPCPKGTRGI ASFQCLPALG LWNPRGPDLS NCTSPWVNQV 

       550        560        570        580        590        600 
AQKIKSGENA ANIASELARH TRGSIYAGDV SSSVKLMEQL LDILDAQLQA LRPIERESAG 

       610        620        630        640        650        660 
KNYNKMHKRE RTCKDYIKAV VETVDNLLRP EALESWKDMN ATEQVHTATM LLDVLEEGAF 

       670        680        690        700        710        720 
LLADNVREPA RFLAAKENVV LEVTVLNTEG QVQELVFPQE EYPRKNSIQL SAKTIKQNSR 

       730        740        750        760        770        780 
NGVVKVVFIL YNNLGLFLST ENATVKLAGE AGPGGPGGAS LVVNSQVIAA SINKESSRVF 

       790        800        810        820        830        840 
LMDPVIFTVA HLEDKNHFNA NCSFWNYSER SMLGYWSTQG CRLVESNKTH TTCACSHLTN 

       850        860        870        880        890        900 
FAVLMAHREI YQGRINELLL SVITWVGIVI SLVCLAICIS TFCFLRGLQT DRNTIHKNLC 

       910        920        930        940        950        960 
INLFLAELLF LVGIDKTQYE IACPIFAGLL HYFFLAAFSW LCLEGVHLYL LLVEVFESEY 

       970        980        990       1000       1010       1020 
SRTKYYYLGG YCFPALVVGI AAAIDYRSYG TEKACWLRVD NYFIWSFIGP VSFVIVVNLV 

      1030       1040       1050       1060       1070       1080 
FLMVTLHKMI RSSSVLKPDS SRLDNIKSWA LGAIALLFLL GLTWAFGLLF INKESVVMAY 

      1090       1100       1110       1120       1130       1140 
LFTTFNAFQG VFIFVFHCAL QKKVHKEYSK CLRHSYCCIR SPPGGTHGSL KTSAMRSNTR 

      1150       1160       1170       1180       1190       1200 
YYTGTQSRIR RMWNDTVRKQ TESSFMAGDI NSTPTLNRGT MGNHLLTNPV LQPRGGTSPY 

      1210       1220       1230       1240       1250       1260 
NTLIAESVGF NPSSPPVFNS PGSYREPKHP LGGREACGMD TLPLNGNFNN SYSLRSGDFP 

      1270       1280       1290       1300       1310       1320 
PGDGGPEPPR GRNLADAAAF EKMIISELVH NNLRGSSSAA KGPPPPEPPV PPVPGGGGEE 

      1330       1340       1350       1360       1370       1380 
EAGGPGGADR AEIELLYKAL EEPLLLPRAQ SVLYQSDLDE SESCTAEDGA TSRPLSSPPG 

      1390       1400       1410       1420       1430       1440 
RDSLYASGAN LRDSPSYPDS SPEGPSEALP PPPPAPPGPP EIYYTSRPPA LVARNPLQGY 

      1450       1460       1470 
YQVRRPSHEG YLAAPGLEGP GPDGDGQMQL VTSL 

« Hide

Isoform 2 [UniParc].

Checksum: 4907D329A56E57FF
Show »

FASTA1,469162,118

References

« Hide 'large scale' references
[1]Douangpanya J., Puri K., Hayflick J.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 636-1474.
Tissue: Brain and Lung.
[5]"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF307079 mRNA. Translation: AAG27461.1.
AB020628 mRNA. Translation: BAA74844.2. Different initiation.
AB065919 Genomic DNA. Translation: BAC06134.1.
BC002974 mRNA. Translation: AAH02974.1.
BC007587 mRNA. Translation: AAH07587.1.
RefSeqNP_001008701.1. NM_001008701.2.
NP_055736.2. NM_014921.4.
UniGeneHs.94229.

3D structure databases

ProteinModelPortalO94910.
SMRO94910. Positions 29-134, 474-838, 861-1100.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116528. 4 interactions.
IntActO94910. 2 interactions.
STRING9606.ENSP00000340688.

Protein family/group databases

MEROPSS63.013.
GPCRDBSearch...

PTM databases

PhosphoSiteO94910.

Proteomic databases

PaxDbO94910.
PRIDEO94910.

Protocols and materials databases

DNASU22859.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000340736; ENSP00000340688; ENSG00000072071. [O94910-1]
ENST00000361434; ENSP00000355328; ENSG00000072071. [O94910-2]
GeneID22859.
KEGGhsa:22859.
UCSCuc010xnn.2. human. [O94910-1]
uc010xno.2. human. [O94910-2]

Organism-specific databases

CTD22859.
GeneCardsGC19M014258.
HGNCHGNC:20973. LPHN1.
HPAHPA037974.
neXtProtNX_O94910.
PharmGKBPA134868822.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG253931.
HOGENOMHOG000049065.
HOVERGENHBG052337.
InParanoidO94910.
KOK04592.
OMADRIYVMP.
OrthoDBEOG76DTRK.
PhylomeDBO94910.
TreeFamTF351999.

Gene expression databases

ArrayExpressO94910.
BgeeO94910.
CleanExHS_LPHN1.
GenevestigatorO94910.

Family and domain databases

InterProIPR022624. DUF3497.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF12003. DUF3497. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
PRINTSPR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
[Graphical view]
PROSITEPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiLPHN1.
GenomeRNAi22859.
NextBio43351.
PROO94910.

Entry information

Entry nameLPHN1_HUMAN
AccessionPrimary (citable) accession number: O94910
Secondary accession number(s): Q96IE7, Q9BU07, Q9HAR3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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