Reviewed, UniProtKB/Swiss-Prot O94906 (PRP6_HUMAN)

Names and origin

Protein names

Recommended name:
    Pre-mRNA-processing factor 6
Alternative name(s):
    U5 snRNP-associated 102 kDa protein
      Short name=U5-102 kDa protein
    PRP6 homolog

Gene names

Name:	PRPF6
Synonyms:	C20orf14

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	941 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation.
Subunit structure	Associates with the U5 snRNP particle (containing the 40 kDa, 52 kDa, 116 kDa, 200 kDa and 220 kDa proteins) probably via interaction(s) with the 220 kDa and/or other proteins. Associates with U4/U6 snRNP particle (containing the 15.5 kDa, 20 kDa/60kDa/90kDa heteromer, LSm proteins LSm2-8, 61 kDa and Sm proteins). Interacts with ARAF1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Ref.2 Ref.3
Subcellular location	Nucleus. Ref.1
Sequence similarities	Contains 9 HAT repeats.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI spliceosome assembly Ref.2 Non-traceable author statement. Source: UniProtKB
Cellular component	spliceosome Ref.2 Non-traceable author statement. Source: UniProtKB
Molecular function	RNA splicing factor activity, transesterification mechanism Ref.2 Non-traceable author statement. Source: UniProtKB androgen receptor binding Inferred from physical interaction. Source: MGI transcription coactivator activity Inferred from genetic interaction. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARAF	P10398	4	EBI-536755,EBI-365961
CD2BP2	O95400	1	EBI-536755,EBI-768015
RNPS1	Q15287	1	EBI-536755,EBI-395959
SMARCA2	P51531	1	EBI-536755,EBI-679562
SNRNP40	Q96DI7	1	EBI-536755,EBI-538492

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 941	941	Pre-mRNA-processing factor 6		PRO_0000205759
Regions
	Repeat	384 – 416	33	HAT 1
	Repeat	418 – 444	27	HAT 2
	Repeat	445 – 476	32	HAT 3
	Repeat	554 – 586	33	HAT 4
	Repeat	588 – 620	33	HAT 5
	Repeat	622 – 654	33	HAT 6
	Repeat	689 – 721	33	HAT 7
	Repeat	723 – 755	33	HAT 8
	Repeat	855 – 887	33	HAT 9
	Compositional bias	67 – 72	6	Poly-Asp
Amino acid modifications
	Modified residue	266	1	Phosphothreonine Ref.8
	Modified residue	275	1	Phosphothreonine Ref.8
	Modified residue	279	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	20	1	G → W in AAD01798. Ref.3
	Sequence conflict	223	1	P → R in AAD01798. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O94906-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 91C2F1ADCA439BE9	FASTA	941	106,925
	10 20 30 40 50 60 MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM 70 80 90 100 110 120 KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE ADAIYAALDK RMDERRKERR 130 140 150 160 170 180 EQREKEEIEK YRMERPKIQQ QFSDLKRKLA EVTEEEWLSI PEVGDARNKR QRNPRYEKLT 190 200 210 220 230 240 PVPDSFFAKH LQTGENHTSV DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM 250 260 270 280 290 300 RKIGQARNTL MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS 310 320 330 340 350 360 VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA RLQPGDTAKA 370 380 390 400 410 420 VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV PNSVRLWKAA VELEEPEDAR 430 440 450 460 470 480 IMLSRAVECC PTSVELWLAL ARLETYENAR KVLNKARENI PTDRHIWITA AKLEEANGNT 490 500 510 520 530 540 QMVEKIIDRA ITSLRANGVE INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK 550 560 570 580 590 600 HTWMEDADSC VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA 610 620 630 640 650 660 VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK LESENDEYER 670 680 690 700 710 720 ARRLLAKARS SAPTARVFMK SVKLEWVQDN IRAAQDLCEE ALRHYEDFPK LWMMKGQIEE 730 740 750 760 770 780 QKEMMEKARE AYNQGLKKCP HSTPLWLLLS RLEEKIGQLT RARAILEKSR LKNPKNPGLW 790 800 810 820 830 840 LESVRLEYRA GLKNIANTLM AKALQECPNS GILWSEAIFL EARPQRRTKS VDALKKCEHD 850 860 870 880 890 900 PHVLLAVAKL FWSQRKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR 910 920 930 940 CESAEPRHGE LWCAVSKDIA NWQKKIGDIL RLVAGRIKNT F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel mammalian nuclear protein similar to Schizosaccharomyces pombe Prp1p/Zer1p and Saccharomyces cerevisiae Prp6p pre-mRNA splicing factors." Nishikimi A., Mukai J., Kioka N., Yamada M. Biochim. Biophys. Acta 1435:147-152(1999) [PubMed: 10561546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 138-147; 300-309; 352-359; 776-784 AND 891-898, SUBCELLULAR LOCATION. Tissue: Cervix carcinoma.
[2]	"The human homologue of the yeast splicing factor Prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions." Makarov E.M., Makarova O.V., Achsel T., Luehrmann R. J. Mol. Biol. 298:567-575(2000) [PubMed: 10788320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 324-334; 395-408; 755-768; 871-883 AND 917-924, INTERACTION WITH U5 AND U4/U6 SNRNPS. Tissue: Cervix carcinoma.
[3]	"Isoform-specific localization of A-RAF in mitochondria." Yuryev A., Ono M., Goff S.A., Macaluso F., Wennogle L.P. Mol. Cell. Biol. 20:4870-4878(2000) [PubMed: 10848612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ARAF1. Tissue: Cervix carcinoma.
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 402-941. Tissue: Testis.
[7]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed: 11991638] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-266; THR-275 AND SER-279, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB019219 mRNA. Translation: BAA37140.1. AF221842 mRNA. Translation: AAF66128.1. AF026031 mRNA. Translation: AAD01798.1. AL356790, AL118506, AL355803 Genomic DNA. Translation: CAH71582.1. AL118506, AL355803, AL356790 Genomic DNA. Translation: CAI21906.1. AL355803, AL118506, AL356790 Genomic DNA. Translation: CAC16610.2. BC001666 mRNA. Translation: AAH01666.1. AL137320 mRNA. Translation: CAB70695.1.
IPI	IPI00305068.
PIR	T46386.
RefSeq	NP_036601.2.
UniGene	Hs.31334
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:29006N.
IntAct	O94906. 9 interactions.
PTM databases
PhosphoSite	O94906.
Proteomic databases
PeptideAtlas	O94906.
PRIDE	O94906.
Genome annotation databases
Ensembl	ENSG00000101161. Homo sapiens. [Contig view]
GeneID	24148.
KEGG	hsa:24148.
NMPDR	fig|9606.3.peg.20721.
UCSC	uc002yho.1. human.
Organism-specific databases
GeneCards	GC20P062083. GC20P062085.
H-InvDB	HIX0016017.
HGNC	HGNC:15860. PRPF6.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O94906.
OMA	O94906. PTHGGDI.
Enzyme and pathway databases
Reactome	REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	O94906.
Bgee	O94906.
CleanEx	HS_PRPF6.
GermOnline	ENSG00000101161. Homo sapiens.
Family and domain databases
InterPro	IPR003107. HAT. IPR010491. PRP1_N. IPR001440. TPR-1. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 3 hits.
Pfam	PF06424. PRP1_N. 1 hit. PF00515. TPR_1. 1 hit. [Graphical view]
SMART	SM00386. HAT. 13 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	46847.

Entry information

Entry name

PRP6_HUMAN

Accession

Primary (citable) accession number: O94906
Secondary accession number(s): O95109 , Q5VXS5, Q9H3Z1, Q9H4T9, Q9H4U8, Q9NTE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	May 1, 1999
Last modified:	July 7, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)