Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SAM and SH3 domain-containing protein 1

Gene

SASH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in a signaling pathway. Could act as a tumor suppressor.

GO - Molecular functioni

  • mitogen-activated protein kinase kinase kinase binding Source: MGI
  • protein complex scaffold Source: MGI
  • protein C-terminus binding Source: MGI
  • protein kinase binding Source: MGI

GO - Biological processi

  • positive regulation of angiogenesis Source: MGI
  • positive regulation of endothelial cell migration Source: MGI
  • positive regulation of JUN kinase activity Source: MGI
  • positive regulation of lipopolysaccharide-mediated signaling pathway Source: MGI
  • positive regulation of NIK/NF-kappaB signaling Source: MGI
  • positive regulation of p38MAPK cascade Source: MGI
  • protein polyubiquitination Source: MGI
  • regulation of protein autoubiquitination Source: MGI
  • regulation of protein K63-linked ubiquitination Source: MGI
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000111961-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SAM and SH3 domain-containing protein 1
Alternative name(s):
Proline-glutamate repeat-containing protein
Gene namesi
Name:SASH1
Synonyms:KIAA0790, PEPE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:19182. SASH1.

Subcellular locationi

GO - Cellular componenti

  • protein complex Source: MGI
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi23328.
OpenTargetsiENSG00000111961.
PharmGKBiPA134984521.

Polymorphism and mutation databases

BioMutaiSASH1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975971 – 1247SAM and SH3 domain-containing protein 1Add BLAST1247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90PhosphoserineCombined sources1
Modified residuei248PhosphoserineBy similarity1
Modified residuei407PhosphoserineCombined sources1
Modified residuei614PhosphoserineCombined sources1
Modified residuei821PhosphoserineBy similarity1
Modified residuei839PhosphoserineBy similarity1
Modified residuei858PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO94885.
MaxQBiO94885.
PaxDbiO94885.
PeptideAtlasiO94885.
PRIDEiO94885.

PTM databases

iPTMnetiO94885.
PhosphoSitePlusiO94885.

Expressioni

Tissue specificityi

Expressed ubiquitously, with highest levels in lung, placenta, spleen and thymus. Down-regulated in the majority (74%) of breast tumors in comparison with corresponding normal breast epithelial tissues.1 Publication

Gene expression databases

BgeeiENSG00000111961.
CleanExiHS_SASH1.
ExpressionAtlasiO94885. baseline and differential.
GenevisibleiO94885. HS.

Organism-specific databases

HPAiHPA029947.
HPA029949.

Interactioni

GO - Molecular functioni

  • mitogen-activated protein kinase kinase kinase binding Source: MGI
  • protein complex scaffold Source: MGI
  • protein C-terminus binding Source: MGI
  • protein kinase binding Source: MGI

Protein-protein interaction databases

BioGridi116916. 12 interactors.
IntActiO94885. 7 interactors.
MINTiMINT-1195945.
STRINGi9606.ENSP00000356437.

Structurei

Secondary structure

11247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi556 – 561Combined sources6
Beta strandi582 – 587Combined sources6
Beta strandi590 – 592Combined sources3
Beta strandi594 – 597Combined sources4
Beta strandi602 – 605Combined sources4
Beta strandi610 – 612Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DL0NMR-A1164-1247[»]
2EBPNMR-A555-614[»]
ProteinModelPortaliO94885.
SMRiO94885.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO94885.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini557 – 614SH3Add BLAST58
Domaini633 – 697SAM 1PROSITE-ProRule annotationAdd BLAST65
Domaini1177 – 1241SAM 2PROSITE-ProRule annotationAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 33Pro-richAdd BLAST25
Compositional biasi978 – 1059Pro-richAdd BLAST82

Sequence similaritiesi

Contains 2 SAM (sterile alpha motif) domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.Curated

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410INAU. Eukaryota.
ENOG410ZDYS. LUCA.
GeneTreeiENSGT00390000014672.
HOGENOMiHOG000139718.
HOVERGENiHBG058816.
InParanoidiO94885.
OMAiCETLEGP.
OrthoDBiEOG091G0BS1.
PhylomeDBiO94885.
TreeFamiTF350709.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
InterProiIPR021090. rSAM/SH3_domain-containing.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
PF07647. SAM_2. 1 hit.
PF07653. SH3_2. 1 hit.
PF12485. SLY. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 2 hits.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDAGAAGPG PEPEPEPEPE PEPAPEPEPE PKPGAGTSEA FSRLWTDVMG
60 70 80 90 100
ILDGSLGNID DLAQQYADYY NTCFSDVCER MEELRKRRVS QDLEVEKPDA
110 120 130 140 150
SPTSLQLRSQ IEESLGFCSA VSTPEVERKN PLHKSNSEDS SVGKGDWKKK
160 170 180 190 200
NKYFWQNFRK NQKGIMRQTS KGEDVGYVAS EITMSDEERI QLMMMVKEKM
210 220 230 240 250
ITIEEALARL KEYEAQHRQS AALDPADWPD GSYPTFDGSS NCNSREQSDD
260 270 280 290 300
ETEESVKFKR LHKLVNSTRR VRKKLIRVEE MKKPSTEGGE EHVFENSPVL
310 320 330 340 350
DERSALYSGV HKKPLFFDGS PEKPPEDDSD SLTTSPSSSS LDTWGAGRKL
360 370 380 390 400
VKTFSKGESR GLIKPPKKMG TFFSYPEEEK AQKVSRSLTE GEMKKGLGSL
410 420 430 440 450
SHGRTCSFGG FDLTNRSLHV GSNNSDPMGK EGDFVYKEVI KSPTASRISL
460 470 480 490 500
GKKVKSVKET MRKRMSKKYS SSVSEQDSGL DGMPGSPPPS QPDPEHLDKP
510 520 530 540 550
KLKAGGSVES LRSSLSGQSS MSGQTVSTTD SSTSNRESVK SEDGDDEEPP
560 570 580 590 600
YRGPFCGRAR VHTDFTPSPY DTDSLKLKKG DIIDIISKPP MGTWMGLLNN
610 620 630 640 650
KVGTFKFIYV DVLSEDEEKP KRPTRRRRKG RPPQPKSVED LLDRINLKEH
660 670 680 690 700
MPTFLFNGYE DLDTFKLLEE EDLDELNIRD PEHRAVLLTA VELLQEYDSN
710 720 730 740 750
SDQSGSQEKL LVDSQGLSGC SPRDSGCYES SENLENGKTR KASLLSAKSS
760 770 780 790 800
TEPSLKSFSR NQLGNYPTLP LMKSGDALKQ GQEEGRLGGG LAPDTSKSCD
810 820 830 840 850
PPGVTGLNKN RRSLPVSICR SCETLEGPQT VDTWPRSHSL DDLQVEPGAE
860 870 880 890 900
QDVPTEVTEP PPQIVPEVPQ KTTASSTKAQ PLEQDSAVDN ALLLTQSKRF
910 920 930 940 950
SEPQKLTTKK LEGSIAASGR GLSPPQCLPR NYDAQPPGAK HGLARTPLEG
960 970 980 990 1000
HRKGHEFEGT HHPLGTKEGV DAEQRMQPKI PSQPPPVPAK KSRERLANGL
1010 1020 1030 1040 1050
HPVPMGPSGA LPSPDAPCLP VKRGSPASPT SPSDCPPALA PRPLSGQAPG
1060 1070 1080 1090 1100
SPPSTRPPPW LSELPENTSL QEHGVKLGPA LTRKVSCARG VDLETLTENK
1110 1120 1130 1140 1150
LHAEGIDLTE EPYSDKHGRC GIPEALVQRY AEDLDQPERD VAANMDQIRV
1160 1170 1180 1190 1200
KQLRKQHRMA IPSGGLTEIC RKPVSPGCIS SVSDWLISIG LPMYAGTLST
1210 1220 1230 1240
AGFSTLSQVP SLSHTCLQEA GITEERHIRK LLSAARLFKL PPGPEAM
Length:1,247
Mass (Da):136,653
Last modified:April 17, 2007 - v3
Checksum:i48A806B3AE32E563
GO

Sequence cautioni

The sequence BAA34510 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14909 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti751T → A in BAB14909 (PubMed:14702039).Curated1
Sequence conflicti1157H → R in BAB14909 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031714298P → Q.Corresponds to variant rs35078400dbSNPEnsembl.1
Natural variantiVAR_031715884Q → R.3 PublicationsCorresponds to variant rs208696dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507735 mRNA. Translation: CAD47811.1.
BN000088 mRNA. Translation: CAD92036.1.
AY351979
, AY351960, AY351961, AY351962, AY351963, AY351964, AY351965, AY351966, AY351967, AY351968, AY351969, AY351970, AY351971, AY351972, AY351973, AY351974, AY351975, AY351976, AY351977, AY351978 Genomic DNA. Translation: AAQ55463.1.
AB018333 mRNA. Translation: BAA34510.1. Different initiation.
AL513164, AL033378 Genomic DNA. Translation: CAH73449.1.
AL033378, AL513164 Genomic DNA. Translation: CAI20166.1.
AK024403 mRNA. Translation: BAB14909.1. Different initiation.
CH471051 Genomic DNA. Translation: EAW47815.1.
BC028303 mRNA. Translation: AAH28303.1.
CCDSiCCDS5212.1.
RefSeqiNP_056093.3. NM_015278.3.
UniGeneiHs.193133.

Genome annotation databases

EnsembliENST00000367467; ENSP00000356437; ENSG00000111961.
GeneIDi23328.
KEGGihsa:23328.
UCSCiuc003qme.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507735 mRNA. Translation: CAD47811.1.
BN000088 mRNA. Translation: CAD92036.1.
AY351979
, AY351960, AY351961, AY351962, AY351963, AY351964, AY351965, AY351966, AY351967, AY351968, AY351969, AY351970, AY351971, AY351972, AY351973, AY351974, AY351975, AY351976, AY351977, AY351978 Genomic DNA. Translation: AAQ55463.1.
AB018333 mRNA. Translation: BAA34510.1. Different initiation.
AL513164, AL033378 Genomic DNA. Translation: CAH73449.1.
AL033378, AL513164 Genomic DNA. Translation: CAI20166.1.
AK024403 mRNA. Translation: BAB14909.1. Different initiation.
CH471051 Genomic DNA. Translation: EAW47815.1.
BC028303 mRNA. Translation: AAH28303.1.
CCDSiCCDS5212.1.
RefSeqiNP_056093.3. NM_015278.3.
UniGeneiHs.193133.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DL0NMR-A1164-1247[»]
2EBPNMR-A555-614[»]
ProteinModelPortaliO94885.
SMRiO94885.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116916. 12 interactors.
IntActiO94885. 7 interactors.
MINTiMINT-1195945.
STRINGi9606.ENSP00000356437.

PTM databases

iPTMnetiO94885.
PhosphoSitePlusiO94885.

Polymorphism and mutation databases

BioMutaiSASH1.

Proteomic databases

EPDiO94885.
MaxQBiO94885.
PaxDbiO94885.
PeptideAtlasiO94885.
PRIDEiO94885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367467; ENSP00000356437; ENSG00000111961.
GeneIDi23328.
KEGGihsa:23328.
UCSCiuc003qme.2. human.

Organism-specific databases

CTDi23328.
DisGeNETi23328.
GeneCardsiSASH1.
H-InvDBHIX0006282.
HGNCiHGNC:19182. SASH1.
HPAiHPA029947.
HPA029949.
MIMi607955. gene.
neXtProtiNX_O94885.
OpenTargetsiENSG00000111961.
PharmGKBiPA134984521.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INAU. Eukaryota.
ENOG410ZDYS. LUCA.
GeneTreeiENSGT00390000014672.
HOGENOMiHOG000139718.
HOVERGENiHBG058816.
InParanoidiO94885.
OMAiCETLEGP.
OrthoDBiEOG091G0BS1.
PhylomeDBiO94885.
TreeFamiTF350709.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000111961-MONOMER.

Miscellaneous databases

ChiTaRSiSASH1. human.
EvolutionaryTraceiO94885.
GeneWikiiSASH1.
GenomeRNAii23328.
PROiO94885.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111961.
CleanExiHS_SASH1.
ExpressionAtlasiO94885. baseline and differential.
GenevisibleiO94885. HS.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
InterProiIPR021090. rSAM/SH3_domain-containing.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
PF07647. SAM_2. 1 hit.
PF07653. SH3_2. 1 hit.
PF12485. SLY. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 2 hits.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSASH1_HUMAN
AccessioniPrimary (citable) accession number: O94885
Secondary accession number(s): Q5TGN5, Q8TAI0, Q9H7R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: April 17, 2007
Last modified: November 2, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.