O94880 (PHF14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PHD finger protein 14

Gene names

Name:	PHF14
Synonyms:	KIAA0783

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	888 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11
Sequence similarities	Contains 2 PHD-type zinc fingers.
Sequence caution	The sequence BAA34503.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94880-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94880-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-285: Missing.
     286-300: LTNDSLTLSQSKSNE → MIVQMTVILKLEMPQ
     886-888: YPS → CDECRLCYHFGCLDPPLKKSPKQTGYGWICQECDSSSSKEDENEAERKNISQELNMEQKNPKK

Note: Phosphorylated on Ser-651.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 888	888	PHD finger protein 14		PRO_0000059306
Regions
	Zinc finger	319 – 380	62	PHD-type 1
	Zinc finger	725 – 779	55	PHD-type 2
	Compositional bias	62 – 137	76	Glu/Lys-rich
Amino acid modifications
	Modified residue	91	1	Phosphoserine Ref.8
	Modified residue	208	1	Phosphoserine Ref.9
	Modified residue	280	1	Phosphoserine Ref.9
	Modified residue	287	1	Phosphothreonine Ref.7 Ref.9 Ref.10 Ref.11
	Modified residue	290	1	Phosphoserine Ref.6 Ref.7 Ref.9 Ref.10 Ref.11
	Modified residue	294	1	Phosphoserine Ref.9
	Modified residue	298	1	Phosphoserine Ref.7
	Modified residue	302	1	Phosphoserine Ref.7 Ref.11
	Modified residue	530	1	Phosphoserine Ref.8
	Modified residue	553	1	Phosphoserine Ref.7
	Modified residue	835	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 285	285	Missing in isoform 2.		VSP_035999
	Alternative sequence	286 – 300	15	LTNDS…SKSNE → MIVQMTVILKLEMPQ in isoform 2.		VSP_036000
	Alternative sequence	886 – 888	3	YPS → CDECRLCYHFGCLDPPLKKS PKQTGYGWICQECDSSSSKE DENEAERKNISQELNMEQKN PKK in isoform 2.		VSP_036001
	Natural variant	115	1	K → R. Ref.1 Corresponds to variant rs218966 [ dbSNP | Ensembl ].		VAR_018480
Experimental info
	Sequence conflict	395	1	F → S in BAG58394. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 24, 2009. Version 2. Checksum: DC356A84BE525932	FASTA	888	100,053
	10 20 30 40 50 60 MDRSSKRRQV KPLAASLLEA LDYDSSDDSD FKVGDASDSE GSGNGSEDAS KDSGEGSCSD 70 80 90 100 110 120 SEENILEEEL NEDIKVKEEQ LKNSAEEEVL SSEKQLIKME KKEEEENGER PRKKKEKEKE 130 140 150 160 170 180 KEKEKEKEKE REKEKEKATV SENVAASAAA TTPATSPPAV NTSPSVPTTT TATEEQVSEP 190 200 210 220 230 240 KKWNLRRNRP LLDFVSMEEL NDMDDYDSED DNDWRPTVVK RKGRSASQKE GSDGDNEDDE 250 260 270 280 290 300 DEGSGSDEDE NDEGNDEDHS SPASEGGCKK KKSKVLSRNS ADDEELTNDS LTLSQSKSNE 310 320 330 340 350 360 DSLILEKSQN WSSQKMDHIL ICCVCLGDNS EDADEIIQCD NCGITVHEGC YGVDGESDSI 370 380 390 400 410 420 MSSASENSTE PWFCDACKCG VSPSCELCPN QDGIFKETDA GRWVHIVCAL YVPGVAFGDI 430 440 450 460 470 480 DKLRPVTLTE MNYSKYGAKE CSFCEDPRFA RTGVCISCDA GMCRAYFHVT CAQKEGLLSE 490 500 510 520 530 540 AAAEEDIADP FFAYCKQHAD RLDRKWKRKN YLALQSYCKM SLQEREKQLS PEAQARINAR 550 560 570 580 590 600 LQQYRAKAEL ARSTRPQAWV PREKLPRPLT SSASAIRKLM RKAELMGIST DIFPVDNSDT 610 620 630 640 650 660 SSSVDGRRKH KQPALTADFV NYYFERNMRM IQIQENMAEQ KNIKDKLENE QEKLHVEYNK 670 680 690 700 710 720 LCESLEELQN LNGKLRSEGQ GIWALLGRIT GQKLNIPAIL RAPKERKPSK KEGGTQKTST 730 740 750 760 770 780 LPAVLYSCGI CKKNHDQHLL LLCDTCKLHY HLGCLDPPLT RMPRKTKNSY WQCSECDQAG 790 800 810 820 830 840 SSDMEADMAM ETLPDGTKRS RRQIKEPVKF VPQDVPPEPK KIPIRNTRTR GRKRSFVPEE 850 860 870 880 EKHEERVPRE RRQRQSVLQK KPKAEDLRTE CATCKGTGDN ENLVRYPS
	Isoform 2 [UniParc]. Checksum: 5B081B62F5792D16	FASTA	663	75,504
	10 20 30 40 50 60 MIVQMTVILK LEMPQDSLIL EKSQNWSSQK MDHILICCVC LGDNSEDADE IIQCDNCGIT 70 80 90 100 110 120 VHEGCYGVDG ESDSIMSSAS ENSTEPWFCD ACKCGVSPSC ELCPNQDGIF KETDAGRWVH 130 140 150 160 170 180 IVCALYVPGV AFGDIDKLRP VTLTEMNYSK YGAKECSFCE DPRFARTGVC ISCDAGMCRA 190 200 210 220 230 240 YFHVTCAQKE GLLSEAAAEE DIADPFFAYC KQHADRLDRK WKRKNYLALQ SYCKMSLQER 250 260 270 280 290 300 EKQLSPEAQA RINARLQQYR AKAELARSTR PQAWVPREKL PRPLTSSASA IRKLMRKAEL 310 320 330 340 350 360 MGISTDIFPV DNSDTSSSVD GRRKHKQPAL TADFVNYYFE RNMRMIQIQE NMAEQKNIKD 370 380 390 400 410 420 KLENEQEKLH VEYNKLCESL EELQNLNGKL RSEGQGIWAL LGRITGQKLN IPAILRAPKE 430 440 450 460 470 480 RKPSKKEGGT QKTSTLPAVL YSCGICKKNH DQHLLLLCDT CKLHYHLGCL DPPLTRMPRK 490 500 510 520 530 540 TKNSYWQCSE CDQAGSSDME ADMAMETLPD GTKRSRRQIK EPVKFVPQDV PPEPKKIPIR 550 560 570 580 590 600 NTRTRGRKRS FVPEEEKHEE RVPRERRQRQ SVLQKKPKAE DLRTECATCK GTGDNENLVR 610 620 630 640 650 660 CDECRLCYHF GCLDPPLKKS PKQTGYGWIC QECDSSSSKE DENEAERKNI SQELNMEQKN PKK

References

[1]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-115. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hippocampus.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-290, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-287; SER-290; SER-298; SER-302; SER-553 AND SER-835, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91 AND SER-530, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208; SER-280; THR-287; SER-290 AND SER-294, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 (ISOFORM 2), MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-287 AND SER-290, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-287; SER-290 AND SER-302, MASS SPECTROMETRY. Tissue: Leukemic T-cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018326 mRNA. Translation: BAA34503.2. Different initiation. AK295461 mRNA. Translation: BAG58394.1. AC005007 Genomic DNA. No translation available. AC007029 Genomic DNA. No translation available. AC080064 Genomic DNA. No translation available. AC104089 Genomic DNA. No translation available. CH471073 Genomic DNA. Translation: EAW93634.1. BC152414 mRNA. Translation: AAI52415.1.
IPI	IPI00472782. IPI00941211.
RefSeq	NP_055475.2. NM_014660.3.
UniGene	Hs.655688.
3D structure databases
ProteinModelPortal	O94880.
SMR	O94880. Positions 317-408, 688-780.
ModBase	Search...
Protein-protein interaction databases
IntAct	O94880. 2 interactions.
STRING	O94880.
Proteomic databases
PRIDE	O94880.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000403050; ENSP00000385795; ENSG00000106443.
GeneID	9678.
KEGG	hsa:9678.
Organism-specific databases
CTD	9678.
GeneCards	GC07P010980.
H-InvDB	HIX0006485.
HGNC	HGNC:22203. PHF14.
HPA	HPA000538.
neXtProt	NX_O94880.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15559.
GeneTree	ENSGT00600000084089.
HOVERGEN	HBG053584.
OrthoDB	EOG437RD8.
PhylomeDB	O94880.
Gene expression databases
ArrayExpress	O94880.
Bgee	O94880.
CleanEx	HS_PHF14.
Genevestigator	O94880.
GermOnline	ENSG00000106443. Homo sapiens.
Family and domain databases
InterPro	IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 2 hits.
Pfam	PF00628. PHD. 2 hits. [Graphical view]
SMART	SM00249. PHD. 3 hits. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 2 hits.
PROSITE	PS01359. ZF_PHD_1. 2 hits. PS50016. ZF_PHD_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	36345.

Entry information

Entry name

PHF14_HUMAN

Accession

Primary (citable) accession number: O94880
Secondary accession number(s): A7MCZ3, B4DI82

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	November 24, 2009
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.