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O94875 (SRBS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sorbin and SH3 domain-containing protein 2
Alternative name(s):
Arg/Abl-interacting protein 2
Short name=ArgBP2
Sorbin
Gene names
Name:SORBS2
Synonyms:ARGBP2, KIAA0777
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1100 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. Isoform 6 increases water and sodium absorption in the intestine and gall-bladder. Ref.1 Ref.7

Subunit structure

Interacts with ABL, CBL, DNM1, DNM2, FLOT1, MLLT4/afadin, PTK2B/PYK2, SAPAP, SPTAN1, SYNJ1, SYNJ2, VCL/vinculin, and WASF By similarity. Interacts with ABL1/c-Abl, ABL2/v-Abl/Arg, ACTN, AKT1, CBL, PALLD and PAK1. Ref.1 Ref.7 Ref.8 Ref.9

Subcellular location

Cytoplasmperinuclear region. Note: Found at the Z-disk sarcomeres, stress fibers, dense bodies and focal adhesion. Ref.1 Ref.8

Tissue specificity

Abundantly expressed in heart. In cardiac muscle cells, located in the Z-disks of sarcomere. Also found, but to a lower extent, in small and large intestine, pancreas, thymus, colon, spleen, prostate, testis, brain, ovary and epithelial cells. Ref.1 Ref.2

Post-translational modification

Ubiquitinated by CBL. Ref.7 Ref.9

Sequence similarities

Contains 3 SH3 domains.

Contains 1 SoHo domain.

Sequence caution

The sequence BAA34497.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O94875-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O94875-2)

Also known as: ArgBP2a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNTGRDSQSPDSPKGFRSVRPNLQDKRSPTQSQITVNGNSGGAVSPM
     228-228: P → PTDRINPDDIDLENEPWYKFFSELEFGRPPPKKPLDYVQDHSSGVFNE
     308-834: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: O94875-3)

The sequence of this isoform differs from the canonical sequence as follows:
     228-228: P → PPPLPTTPTPVPREPGRKPLSSSRLGEVTGSPSPPPRSGAPTPSSRAPALSPTRPPKKPLDYVQDHSSGVFNE
     308-834: Missing.
Isoform 4 (identifier: O94875-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNTGRDSQSPDSPKGFRSVRPNLQDKRSPTQSQITVNGNSGGAVSPM
     228-228: P → PPPLPTTPTPVPREPGRKPLSSSRLGEVTGSPSPPPRSGAPTPSSRAPALSPTRPPKKPLDYVQDHSSGVFNE
     308-834: Missing.
Isoform 5 (identifier: O94875-5)

Also known as: ArgBP2b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNTGRDSQSPDSPKGFRSVRPNLQDKRSPTQSQITVNGNSGGAVSPM
     228-228: P → PPPLPTTPTPVPREPGRKPLSSSRLGEVTGSPSPPPRSGAPTPSSRAPALSPTRPPKKPLDYVQDHSSGVFNE
     308-834: Missing.
     1050-1100: YNYTPRNEDELELRESDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL → GYTLT
Isoform 6 (identifier: O94875-6)

Also known as: Sorbin;

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.
     82-89: GIPTAIRT → MKATTPLQ
     229-237: ASLYQSSID → VSKPQAGRR
     238-1100: Missing.
Note: Contains alanine amide at position 153.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11001100Sorbin and SH3 domain-containing protein 2
PRO_0000344477

Regions

Domain66 – 12762SoHo
Domain863 – 92260SH3 1
Domain938 – 99962SH3 2
Domain1041 – 110060SH3 3
Compositional bias167 – 18216Pro-rich
Compositional bias640 – 65213His-rich
Compositional bias931 – 9344Poly-Pro

Amino acid modifications

Modified residue301Phosphoserine By similarity
Modified residue1541Phosphoserine By similarity
Modified residue1571Phosphoserine Ref.12
Modified residue2591Phosphoserine Ref.10 Ref.12
Modified residue2771Phosphothreonine Ref.10 Ref.12
Modified residue2781Phosphoserine By similarity
Modified residue2981Phosphoserine By similarity
Modified residue2991Phosphoserine Ref.12
Modified residue3011Phosphoserine Ref.11 Ref.12
Modified residue3021Phosphoserine Ref.12
Modified residue3711Phosphothreonine By similarity
Modified residue3761Phosphoserine By similarity
Modified residue7501Phosphoserine By similarity
Modified residue10171Phosphoserine By similarity
Modified residue10181Phosphoserine By similarity

Natural variations

Alternative sequence1 – 8181Missing in isoform 6.
VSP_034791
Alternative sequence11M → MNTGRDSQSPDSPKGFRSVR PNLQDKRSPTQSQITVNGNS GGAVSPM in isoform 2, isoform 4 and isoform 5.
VSP_034792
Alternative sequence82 – 898GIPTAIRT → MKATTPLQ in isoform 6.
VSP_034793
Alternative sequence2281P → PTDRINPDDIDLENEPWYKF FSELEFGRPPPKKPLDYVQD HSSGVFNE in isoform 2.
VSP_034794
Alternative sequence2281P → PPPLPTTPTPVPREPGRKPL SSSRLGEVTGSPSPPPRSGA PTPSSRAPALSPTRPPKKPL DYVQDHSSGVFNE in isoform 3, isoform 4 and isoform 5.
VSP_034795
Alternative sequence229 – 2379ASLYQSSID → VSKPQAGRR in isoform 6.
VSP_034796
Alternative sequence238 – 1100863Missing in isoform 6.
VSP_034797
Alternative sequence308 – 834527Missing in isoform 2, isoform 3, isoform 4 and isoform 5.
VSP_034798
Alternative sequence1050 – 110051YNYTP…YVKRL → GYTLT in isoform 5.
VSP_034799
Natural variant10481A → V.
Corresponds to variant rs725185 [ dbSNP | Ensembl ].
VAR_045624

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 22, 2008. Version 3.
Checksum: 7E98B196D4DB38E6

FASTA1,100124,108
        10         20         30         40         50         60 
MSYYQRPFSP SAYSLPASLN SSIVMQHGTS LDSTDTYPQH AQSLDGTTSS SIPLYRSSEE 

        70         80         90        100        110        120 
EKRVTVIKAP HYPGIGPVDE SGIPTAIRTT VDRPKDWYKT MFKQIHMVHK PDDDTDMYNT 

       130        140        150        160        170        180 
PYTYNAGLYN PPYSAQSHPA AKTQTYRPLS KSHSDNSPNA FKDASSPVPP PHVPPPVPPL 

       190        200        210        220        230        240 
RPRDRSSTEK HDWDPPDRKV DTRKFRSEPR SIFEYEPGKS SILQHERPAS LYQSSIDRSL 

       250        260        270        280        290        300 
ERPMSSASMA SDFRKRRKSE PAVGPPRGLG DQSASRTSPG RVDLPGSSTT LTKSFTSSSP 

       310        320        330        340        350        360 
SSPSRAKGGD DSKICPSLCS YSGLNGNPSS ELDYCSTYRQ HLDVPRDSPR AISFKNGWQM 

       370        380        390        400        410        420 
ARQNAEIWSS TEETVSPKIK SRSCDDLLND DCDSFPDPKV KSESMGSLLC EEDSKESCPM 

       430        440        450        460        470        480 
AWGSPYVPEV RSNGRSRIRH RSARNAPGFL KMYKKMHRIN RKDLMNSEVI CSVKSRILQY 

       490        500        510        520        530        540 
ESEQQHKDLL RAWSQCSTEE VPRDMVPTRI SEFEKLIQKS KSMPNLGDDM LSPVTLEPPQ 

       550        560        570        580        590        600 
NGLCPKRRFS IEYLLEEENQ SGPPARGRRG CQSNALVPIH IEVTSDEQPR AHVEFSDSDQ 

       610        620        630        640        650        660 
DGVVSDHSDY IHLEGSSFCS ESDFDHFSFT SSESFYGSSH HHHHHHHHHH RHLISSCKGR 

       670        680        690        700        710        720 
CPASYTRFTT MLKHERARHE NTEEPRRQEM DPGLSKLAFL VSPVPFRRKK NSAPKKQTEK 

       730        740        750        760        770        780 
AKCKASVFEA LDSALKDICD QIKAEKKRGS LPDNSILHRL ISELLPDVPE RNSSLRALRR 

       790        800        810        820        830        840 
SPLHQPLHPL PPDGAIHCPP YQNDCGRMPR SASFQDVDTA NSSCHHQDRG GALQDRESPR 

       850        860        870        880        890        900 
SYSSTLTDMG RSAPRERRGT PEKEKLPAKA VYDFKAQTSK ELSFKKGDTV YILRKIDQNW 

       910        920        930        940        950        960 
YEGEHHGRVG IFPISYVEKL TPPEKAQPAR PPPPAQPGEI GEAIAKYNFN ADTNVELSLR 

       970        980        990       1000       1010       1020 
KGDRVILLKR VDQNWYEGKI PGTNRQGIFP VSYVEVVKKN TKGAEDYPDP PIPHSYSSDR 

      1030       1040       1050       1060       1070       1080 
IHSLSSNKPQ RPVFTHENIQ GGGEPFQALY NYTPRNEDEL ELRESDVIDV MEKCDDGWFV 

      1090       1100 
GTSRRTKFFG TFPGNYVKRL 

« Hide

Isoform 2 (ArgBP2a) [UniParc].

Checksum: B549A26DC04782E0
Show »

FASTA66674,804
Isoform 3 [UniParc].

Checksum: 8CDEF6E34D5F972F
Show »

FASTA64571,899
Isoform 4 [UniParc].

Checksum: 0398BFA54873EDF9
Show »

FASTA69176,738
Isoform 5 (ArgBP2b) [UniParc].

Checksum: 068FBE75BFD30A8C
Show »

FASTA64571,196
Isoform 6 (Sorbin) [UniParc].

Checksum: 95AE8B49881FCC12
Show »

FASTA15617,976

References

« Hide 'large scale' references
[1]"ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl-interacting protein, is phosphorylated in v-Abl-transformed cells and localized in stress fibers and cardiocyte Z-disks."
Wang B., Golemis E.A., Kruh G.D.
J. Biol. Chem. 272:17542-17550(1997) [PubMed: 9211900] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 5), NUCLEOTIDE SEQUENCE [MRNA] OF 1-1049 (ISOFORM 4), PHOSPHORYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH ABL1 AND ABL2, FUNCTION.
Tissue: Brain.
[2]"Coding region of the sorbin gene in different species."
Wahbi K., Magaud J.-P., Pansu D., Descroix-Vagne M.
Peptides 22:2045-2053(2001) [PubMed: 11786189] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6), AMIDATION AT ALA-153 (ISOFORM 6), TISSUE SPECIFICITY.
[3]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed: 15815621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Lymph.
[7]"Cbl-ArgBP2 complex mediates ubiquitination and degradation of c-Abl."
Soubeyran P., Barac A., Szymkiewicz I., Dikic I.
Biochem. J. 370:29-34(2003) [PubMed: 12475393] [Abstract]
Cited for: INTERACTION WITH CBL, UBIQUITINATION BY CBL, FUNCTION.
[8]"Involvement of palladin and alpha-actinin in targeting of the Abl/Arg kinase adaptor ArgBP2 to the actin cytoskeleton."
Roenty M., Taivainen A., Moza M., Kruh G.D., Ehler E., Carpen O.
Exp. Cell Res. 310:88-98(2005) [PubMed: 16125169] [Abstract]
Cited for: INTERACTION WITH PALLD AND ACTN, SUBCELLULAR LOCATION.
[9]"ArgBP2gamma interacts with Akt and p21-activated kinase-1 and promotes cell survival."
Yuan Z.-Q., Kim D., Kaneko S., Sussman M., Bokoch G.M., Kruh G.D., Nicosia S.V., Testa J.R., Cheng J.Q.
J. Biol. Chem. 280:21483-21490(2005) [PubMed: 15784622] [Abstract]
Cited for: INTERACTION WITH AKT1 AND PAK1, UBIQUITINATION BY CBL, PHOSPHORYLATION.
[10]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259 AND THR-277, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[11]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[12]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157; SER-259; THR-277; SER-299; SER-301 AND SER-302, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF049884 mRNA. Translation: AAC05508.1.
AF049885 mRNA. Translation: AAC05509.1.
AB018320 mRNA. Translation: BAA34497.2. Different initiation.
AC093797 Genomic DNA. No translation available.
CH471056 Genomic DNA. Translation: EAX04630.1.
CH471056 Genomic DNA. Translation: EAX04631.1.
CH471056 Genomic DNA. Translation: EAX04632.1.
CH471056 Genomic DNA. Translation: EAX04635.1.
BC011883 mRNA. Translation: AAH11883.1.
IPIIPI00477577.
IPI00747799.
IPI00787549.
IPI00900281.
IPI00900295.
IPI01013920.
RefSeqNP_001139142.1. NM_001145670.1.
NP_001139143.1. NM_001145671.1.
NP_001139144.1. NM_001145672.1.
NP_001139145.1. NM_001145673.1.
NP_003594.3. NM_003603.5.
NP_066547.1. NM_021069.4.
UniGeneHs.619806.
Hs.655143.

3D structure databases

HSSPHSSP built from PDB template 2CT3 based on UniProtKB O60504.
ProteinModelPortalO94875.
SMRO94875. Positions 866-1100.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-31634N.
IntActO94875. 8 interactions.
MINTMINT-196404.
STRINGO94875.

PTM databases

PhosphoSiteO94875.

Proteomic databases

PRIDEO94875.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000284776; ENSP00000284776; ENSG00000154556.
ENST00000355634; ENSP00000347852; ENSG00000154556.
ENST00000431808; ENSP00000411764; ENSG00000154556.
ENST00000447992; ENSP00000415118; ENSG00000154556.
GeneID8470.
KEGGhsa:8470.

Organism-specific databases

CTD8470.
GeneCardsGC04M186506.
HGNCHGNC:24098. SORBS2.
HPAHPA036754.
neXtProtNX_O94875.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG17281.
GeneTreeENSGT00550000074287.
HOVERGENHBG108509.
InParanoidO94875.
OrthoDBEOG4J117C.

Gene expression databases

ArrayExpressO94875.
BgeeO94875.
CleanExHS_SORBS2.
GenevestigatorO94875.

Family and domain databases

InterProIPR000108. p67phox.
IPR001452. SH3_domain.
IPR003127. Sorb.
IPR007087. Znf_C2H2.
[Graphical view]
PfamPF00018. SH3_1. 3 hits.
PF02208. Sorb. 1 hit.
[Graphical view]
PRINTSPR00499. P67PHOX.
PR00452. SH3DOMAIN.
SMARTSM00326. SH3. 3 hits.
SM00459. Sorb. 1 hit.
[Graphical view]
SUPFAMSSF50044. SH3. 3 hits.
PROSITEPS50002. SH3. 3 hits.
PS50831. SOHO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio31696.

Entry information

Entry nameSRBS2_HUMAN
AccessionPrimary (citable) accession number: O94875
Secondary accession number(s): A6NEK9 expand/collapse secondary AC list , D3DP62, D3DP63, O60592, O60593, Q96EX0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: January 25, 2012
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Index of protein domains and families