Reviewed, UniProtKB/Swiss-Prot O94864 (ST65G_HUMAN)

Names and origin

Protein names

Recommended name:
    STAGA complex 65 subunit gamma
Alternative name(s):
    STAF65gamma
    SPTF-associated factor 65 gamma
    Suppressor of Ty 7-like
    Adenocarcinoma antigen ART1

Gene names

Name:	SUPT7L
Synonyms:	KIAA0764

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, SUPT7L, GCN5L2, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12 and TAF9.
Subcellular location	Nucleus. Ref.6
Tissue specificity	Expressed at high levels in adenocarcinomas and gliomas and low in esophageal cancers and malignant hematological disease. Also expressed at high level in the thymus, low in peripheral blood mononuclear cells, and lowest in the stomach, small intestine, and skeletal muscle.
Post-translational modification	Sumoylated. Ref.7

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	histone H3 acetylation Ref.6 Inferred from direct assay. Source: UniProtKB maintenance of protein location in nucleus Inferred from direct assay. Source: HGNC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	STAGA complex Ref.6 Inferred from direct assay. Source: UniProtKB
Molecular function	transcription coactivator activity Ref.6 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94864-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94864-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MNLQ → ML

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	STAGA complex 65 subunit gamma		PRO_0000072233
Amino acid modifications
	Modified residue	316	1	Phosphoserine Ref.9
	Modified residue	334	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	1 – 4	4	MNLQ → ML in isoform 2.		VSP_003974

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 59724A96353D44D5	FASTA	414	46,193
	10 20 30 40 50 60 MNLQRYWGEI PISSSQTNRS SFDLLPREFR LVEVHDPPLH QPSANKPKPP TMLDIPSEPC 70 80 90 100 110 120 SLTIHTIQLI QHNRRLRNLI ATAQAQNQQQ TEGVKTEESE PLPSCPGSPP LPDDLLPLDC 130 140 150 160 170 180 KNPNAPFQIR HSDPESDFYR GKGEPVTELS WHSCRQLLYQ AVATILAHAG FDCANESVLE 190 200 210 220 230 240 TLTDVAHEYC LKFTKLLRFA VDREARLGQT PFPDVMEQVF HEVGIGSVLS LQKFWQHRIK 250 260 270 280 290 300 DYHSYMLQIS KQLSEEYERI VNPEKATEDA KPVKIKEEPV SDITFPVSEE LEADLASGDQ 310 320 330 340 350 360 SLPMGVLGAQ SERFPSNLEV EASPQASSAE VNASPLWNLA HVKMEPQESE EGNVSGHGVL 370 380 390 400 410 GSDVFEEPMS GMSEAGIPQS PDDSDSSYGS HSTDSLMGSS PVFNQRCKKR MRKI
	Isoform 2. Checksum: 99A3830B2E4D3E22	FASTA	412	45,951
	10 20 30 40 50 60 MLRYWGEIPI SSSQTNRSSF DLLPREFRLV EVHDPPLHQP SANKPKPPTM LDIPSEPCSL 70 80 90 100 110 120 TIHTIQLIQH NRRLRNLIAT AQAQNQQQTE GVKTEESEPL PSCPGSPPLP DDLLPLDCKN 130 140 150 160 170 180 PNAPFQIRHS DPESDFYRGK GEPVTELSWH SCRQLLYQAV ATILAHAGFD CANESVLETL 190 200 210 220 230 240 TDVAHEYCLK FTKLLRFAVD REARLGQTPF PDVMEQVFHE VGIGSVLSLQ KFWQHRIKDY 250 260 270 280 290 300 HSYMLQISKQ LSEEYERIVN PEKATEDAKP VKIKEEPVSD ITFPVSEELE ADLASGDQSL 310 320 330 340 350 360 PMGVLGAQSE RFPSNLEVEA SPQASSAEVN ASPLWNLAHV KMEPQESEEG NVSGHGVLGS 370 380 390 400 410 DVFEEPMSGM SEAGIPQSPD DSDSSYGSHS TDSLMGSSPV FNQRCKKRMR KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new tumor-rejection antigen recognized by cytotoxic T lymphocytes infiltrating into a lung adenocarcinoma." Nishizaka S., Gomi S., Harada K., Oizumi K., Itoh K., Shichijo S. Cancer Res. 60:4830-4837(2000) [PubMed: 10987294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[6]	"Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo." Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G. Mol. Cell. Biol. 21:6782-6795(2001) [PubMed: 11564863] [Abstract] Cited for: MASS SPECTROMETRY, SUBCELLULAR LOCATION, IDENTIFICATION AS PART OF THE STAGA COMPLEX WITH SUPT3H; GCN5L2; TRRAP; TAF5L; TAF6L; TADA3L; TAF10; TAF12 AND TAF9.
[7]	"Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates." Gocke C.B., Yu H., Kang J. J. Biol. Chem. 280:5004-5012(2005) [PubMed: 15561718] [Abstract] Cited for: SUMOYLATION.
[8]	"Identification of a small TAF complex and its role in the assembly of TAF-containing complexes." Demeny M.A., Soutoglou E., Nagy Z., Scheer E., Janoshazi A., Richardot M., Argentini M., Kessler P., Tora L. PLoS ONE 2:E316-E316(2007) [PubMed: 17375202] [Abstract] Cited for: IDENTIFICATION IN THE TFTC COMPLEX.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316 AND SER-334, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF197954 mRNA. Translation: AAG28523.1. AF224759 mRNA. Translation: AAG47636.1. AB018307 mRNA. Translation: BAA34484.2. Different initiation. CR456983 mRNA. Translation: CAG33264.1. AC074091 Genomic DNA. Translation: AAX93202.1. BC074000 mRNA. Translation: AAH74000.1.
IPI	IPI00006651. IPI00219415.
RefSeq	NP_055675.1.
UniGene	Hs.6232
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O94864. 1 interaction.
PTM databases
PhosphoSite	O94864.
Proteomic databases
PRIDE	O94864.
Genome annotation databases
Ensembl	ENSG00000119760. Homo sapiens. [Contig view]
GeneID	9913.
KEGG	hsa:9913.
Organism-specific databases
GeneCards	GC02M027727.
H-InvDB	HIX0001930.
HGNC	HGNC:30632. SUPT7L.
PharmGKB	PA142670854.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O94864.
HOVERGEN	O94864.
OMA	O94864. LPLDCKN.
Gene expression databases
ArrayExpress	O94864.
Bgee	O94864.
CleanEx	HS_SUPT7L.
GermOnline	ENSG00000119760. Homo sapiens.
Family and domain databases
InterPro	IPR006565. BTP. [Graphical view]
Pfam	PF07524. Bromo_TP. 1 hit. [Graphical view]
SMART	SM00576. BTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	37396.

Entry information

Entry name

ST65G_HUMAN

Accession

Primary (citable) accession number: O94864
Secondary accession number(s): Q6IB21, Q9H2T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)