O94851 (MICA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-methionine sulfoxide oxidase MICAL2
EC=1.14.13.-

Alternative name(s):
Molecule interacting with CasL protein 2
Short name=MICAL-2

Gene names

Name:	MICAL2
Synonyms:	KIAA0750, MICAL2PV1, MICAL2PV2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1124 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin and regulates the SRF signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA. Ref.10 Ref.11
Catalytic activity	[protein]-methionine + NADPH + O2 = [protein]-methionine-sulfoxide + NADP+ + H2O. Ref.11
Cofactor	FAD By similarity.
Enzyme regulation	Specifically inhibited by CCG-1423, a small molecule inhibitor of SRF:MKL1/MRTF-A-dependent transcription. Ref.11
Subunit structure	Interacts with VIM and PLXNA4 By similarity. Interacts with RAB1B. Ref.9
Subcellular location	Nucleus Ref.11.
Sequence similarities	Belongs to the Mical family. Contains 1 CH (calponin-homology) domain. Contains 1 LIM zinc-binding domain.
Sequence caution	The sequence BAA34470.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	LIM domain
Ligand	Actin-binding FAD Flavoprotein Metal-binding NADP Zinc
Molecular function	Monooxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament depolymerization Inferred from direct assay Ref.11. Source: UniProtKB cytoskeleton organization Inferred from direct assay Ref.11. Source: UniProtKB heart development Inferred from sequence or structural similarity. Source: UniProtKB heart looping Inferred from sequence or structural similarity. Source: UniProtKB oxidation-reduction process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription via serum response element binding Inferred from mutant phenotype Ref.11. Source: UniProtKB sulfur oxidation Inferred from direct assay Ref.11. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay Ref.11. Source: UniProtKB
Molecular_function	FAD binding Inferred from sequence or structural similarity. Source: UniProtKB NADPH:sulfur oxidoreductase activity Inferred from direct assay Ref.11. Source: UniProtKB actin binding Inferred from direct assay Ref.11. Source: UniProtKB oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94851-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94851-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-171: Missing.
     740-986: Missing.
     1112-1124: VHFSLPVLHPLLG → GISTSFFRKV...VLRCFPVKLR

Isoform 3 (identifier: O94851-3)

The sequence of this isoform differs from the canonical sequence as follows:
     929-949: Missing.

Isoform 4 (identifier: O94851-4)

The sequence of this isoform differs from the canonical sequence as follows:
     739-928: Missing.
     950-985: Missing.
     1112-1124: VHFSLPVLHPLLG → GISTSFFRKV...VLRCFPVKLR

Isoform 5 (identifier: O94851-5)

The sequence of this isoform differs from the canonical sequence as follows:
     739-928: Missing.

Isoform 6 (identifier: O94851-6)

The sequence of this isoform differs from the canonical sequence as follows:
     739-985: Missing.
     1112-1124: VHFSLPVLHPLLG → GISTSFFRKV...VLRCFPVKLR

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1124

1124

Protein-methionine sulfoxide oxidase MICAL2

PRO_0000075844

Regions

Domain

516 – 619

104

CH

Domain

1000 – 1062

63

LIM zinc-binding

Nucleotide binding

97 – 125

29

FAD By similarity

Region

2 – 494

493

Monooxygenase domain By similarity

Motif

660 – 681

22

Nuclear localization signal Ref.11

Sites

Binding site

97

1

FAD By similarity

Binding site

116

1

FAD By similarity

Binding site

118

1

FAD By similarity

Binding site

123

1

FAD By similarity

Binding site

125

1

FAD By similarity

Binding site

398

1

FAD By similarity

Natural variations

Alternative sequence

1 – 171

171

Missing in isoform 2.

VSP_009859

Alternative sequence

739 – 985

247

Missing in isoform 6.

VSP_042593

Alternative sequence

739 – 928

190

Missing in isoform 4 and isoform 5.

VSP_042594

Alternative sequence

740 – 986

247

Missing in isoform 2.

VSP_009860

Alternative sequence

929 – 949

21

Missing in isoform 3.

VSP_042595

Alternative sequence

950 – 985

36

Missing in isoform 4.

VSP_042596

Alternative sequence

1112 – 1124

13

VHFSL…HPLLG → GISTSFFRKVLGWPLRLPRD LCNWMQGLLQAAGLHIRDNA YNYCYMYELLSLGLPLLWAF SEVLAAMYRESEGSLESICN WVLRCFPVKLR in isoform 2, isoform 4 and isoform 6.

VSP_009861

Natural variant

145

1

F → L.
Corresponds to variant rs2706656 [ dbSNP | Ensembl ].

VAR_050155

Natural variant

220

1

I → V.
Corresponds to variant rs2306727 [ dbSNP | Ensembl ].

VAR_021992

Natural variant

687

1

D → E.
Corresponds to variant rs3794084 [ dbSNP | Ensembl ].

VAR_050156

Natural variant

1089

1

R → Q.
Corresponds to variant rs2270515 [ dbSNP | Ensembl ].

VAR_020257

Natural variant

1106

1

L → P. Ref.3
Corresponds to variant rs1027335 [ dbSNP | Ensembl ].

VAR_024523

Natural variant

1110

1

P → S.
Corresponds to variant rs35518829 [ dbSNP | Ensembl ].

VAR_050157

Experimental info

Mutagenesis

95

1

G → V: Blocks FAD binding and abolishes catalytic activity. Ref.11

Mutagenesis

677 – 681

5

KRRRK → AAAAA in MICAL-2NLSMut; abolishes nuclear localization. Ref.11

Sequence conflict

19

1

F → S in BAG57951. Ref.3

Sequence conflict

208

1

D → G in CAD97967. Ref.4

Sequence conflict

296

1

T → A in CAD97967. Ref.4

Sequence conflict

529

1

Q → H in BAG57951. Ref.3

Sequence conflict

563

1

I → V in BAG57951. Ref.3

Sequence conflict

631

1

S → Y in CAD97967. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1124

Helix Strand Turn

Details...

Helix

520 – 530

11

Beta strand

540 – 542

3

Helix

543 – 545

3

Beta strand

546 – 548

3

Helix

549 – 558

10

Turn

560 – 562

3

Turn

565 – 567

3

Helix

570 – 572

3

Helix

573 – 586

14

Helix

596 – 601

6

Helix

607 – 621

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1C276C42D17B64DF	FASTA	1,124	126,689
	10 20 30 40 50 60 MGENEDEKQA QAGQVFENFV QASTCKGTLQ AFNILTRHLD LDPLDHRNFY SKLKSKVTTW 70 80 90 100 110 120 KAKALWYKLD KRGSHKEYKR GKSCTNTKCL IVGGGPCGLR TAIELAYLGA KVVVVEKRDS 130 140 150 160 170 180 FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC AGSIDHISIR QLQLILFKVA LMLGVEIHVN 190 200 210 220 230 240 VEFVKVLEPP EDQENQKIGW RAEFLPTDHS LSEFEFDVII GADGRRNTLE GFRRKEFRGK 250 260 270 280 290 300 LAIAITANFI NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDCTHYFV 310 320 330 340 350 360 MTAKKQSLLD KGVIINDYID TEMLLCAENV NQDNLLSYAR EAADFATNYQ LPSLDFAMNH 370 380 390 400 410 420 YGQPDVAMFD FTCMYASENA ALVRERQAHQ LLVALVGDSL LEPFWPMGTG CARGFLAAFD 430 440 450 460 470 480 TAWMVKSWNQ GTPPLELLAE RESLYRLLPQ TTPENINKNF EQYTLDPGTR YPNLNSHCVR 490 500 510 520 530 540 PHQVKHLYIT KELEHYPLER LGSVRRSVNL SRKESDIRPS KLLTWCQQQT EGYQHVNVTD 550 560 570 580 590 600 LTTSWRSGLA LCAIIHRFRP ELINFDSLNE DDAVENNQLA FDVAEREFGI PPVTTGKEMA 610 620 630 640 650 660 SAQEPDKLSM VMYLSKFYEL FRGTPLRPVD SWRKNYGENA DLSLAKSSIS NNYLNLTFPR 670 680 690 700 710 720 KRTPRVDGQT GENDMNKRRR KGFTNLDEPS NFSSRSLGSN QECGSSKEGG NQNKVKSMAN 730 740 750 760 770 780 QLLAKFEEST RNPSLMKQER RVSGIGKPVL CSSSGPPVHS CCPKPEEATP SPSPPLKRQF 790 800 810 820 830 840 PSVVVTGHVL RELKQVSAGS ECLSRPWRAR AKSDLQLGGT ENFATLPSTR PRAQALSGVL 850 860 870 880 890 900 WRLQQVEEKI LQKRAQNLAN REFHTKNIKE KAAHLASMFG HGDFPQNKLL SKGLSHTHPP 910 920 930 940 950 960 SPPSRLPSPD PAASSSPSTV DSASPARKEK KSPSGFHFHP SHLRTVHPQL TVGKVSSGIG 970 980 990 1000 1010 1020 AAAEVLVNLY MNDHRPKAQA TSPDLESMRK SFPLNLGGSD TCYFCKKRVY VMERLSAEGH 1030 1040 1050 1060 1070 1080 FFHRECFRCS ICATTLRLAA YTFDCDEGKF YCKPHFIHCK TNSKQRKRRA ELKQQREEEA 1090 1100 1110 1120 TWQEQEAPRR DTPTESSCAV AAIGTLEGSP PVHFSLPVLH PLLG
	Isoform 2 [UniParc]. Checksum: 114E5873F13E5C61	FASTA	784	89,890
	10 20 30 40 50 60 MLGVEIHVNV EFVKVLEPPE DQENQKIGWR AEFLPTDHSL SEFEFDVIIG ADGRRNTLEG 70 80 90 100 110 120 FRRKEFRGKL AIAITANFIN RNSTAEAKVE EISGVAFIFN QKFFQDLKEE TGIDLENIVY 130 140 150 160 170 180 YKDCTHYFVM TAKKQSLLDK GVIINDYIDT EMLLCAENVN QDNLLSYARE AADFATNYQL 190 200 210 220 230 240 PSLDFAMNHY GQPDVAMFDF TCMYASENAA LVRERQAHQL LVALVGDSLL EPFWPMGTGC 250 260 270 280 290 300 ARGFLAAFDT AWMVKSWNQG TPPLELLAER ESLYRLLPQT TPENINKNFE QYTLDPGTRY 310 320 330 340 350 360 PNLNSHCVRP HQVKHLYITK ELEHYPLERL GSVRRSVNLS RKESDIRPSK LLTWCQQQTE 370 380 390 400 410 420 GYQHVNVTDL TTSWRSGLAL CAIIHRFRPE LINFDSLNED DAVENNQLAF DVAEREFGIP 430 440 450 460 470 480 PVTTGKEMAS AQEPDKLSMV MYLSKFYELF RGTPLRPVDS WRKNYGENAD LSLAKSSISN 490 500 510 520 530 540 NYLNLTFPRK RTPRVDGQTG ENDMNKRRRK GFTNLDEPSN FSSRSLGSNQ ECGSSKEGGN 550 560 570 580 590 600 QNKVKSMANQ LLAKFEESTR NPSLMKQESM RKSFPLNLGG SDTCYFCKKR VYVMERLSAE 610 620 630 640 650 660 GHFFHRECFR CSICATTLRL AAYTFDCDEG KFYCKPHFIH CKTNSKQRKR RAELKQQREE 670 680 690 700 710 720 EATWQEQEAP RRDTPTESSC AVAAIGTLEG SPPGISTSFF RKVLGWPLRL PRDLCNWMQG 730 740 750 760 770 780 LLQAAGLHIR DNAYNYCYMY ELLSLGLPLL WAFSEVLAAM YRESEGSLES ICNWVLRCFP VKLR
	Isoform 3 [UniParc]. Checksum: EAB33EE2B2DB4BA1	FASTA	1,103	124,253
	10 20 30 40 50 60 MGENEDEKQA QAGQVFENFV QASTCKGTLQ AFNILTRHLD LDPLDHRNFY SKLKSKVTTW 70 80 90 100 110 120 KAKALWYKLD KRGSHKEYKR GKSCTNTKCL IVGGGPCGLR TAIELAYLGA KVVVVEKRDS 130 140 150 160 170 180 FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC AGSIDHISIR QLQLILFKVA LMLGVEIHVN 190 200 210 220 230 240 VEFVKVLEPP EDQENQKIGW RAEFLPTDHS LSEFEFDVII GADGRRNTLE GFRRKEFRGK 250 260 270 280 290 300 LAIAITANFI NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDCTHYFV 310 320 330 340 350 360 MTAKKQSLLD KGVIINDYID TEMLLCAENV NQDNLLSYAR EAADFATNYQ LPSLDFAMNH 370 380 390 400 410 420 YGQPDVAMFD FTCMYASENA ALVRERQAHQ LLVALVGDSL LEPFWPMGTG CARGFLAAFD 430 440 450 460 470 480 TAWMVKSWNQ GTPPLELLAE RESLYRLLPQ TTPENINKNF EQYTLDPGTR YPNLNSHCVR 490 500 510 520 530 540 PHQVKHLYIT KELEHYPLER LGSVRRSVNL SRKESDIRPS KLLTWCQQQT EGYQHVNVTD 550 560 570 580 590 600 LTTSWRSGLA LCAIIHRFRP ELINFDSLNE DDAVENNQLA FDVAEREFGI PPVTTGKEMA 610 620 630 640 650 660 SAQEPDKLSM VMYLSKFYEL FRGTPLRPVD SWRKNYGENA DLSLAKSSIS NNYLNLTFPR 670 680 690 700 710 720 KRTPRVDGQT GENDMNKRRR KGFTNLDEPS NFSSRSLGSN QECGSSKEGG NQNKVKSMAN 730 740 750 760 770 780 QLLAKFEEST RNPSLMKQER RVSGIGKPVL CSSSGPPVHS CCPKPEEATP SPSPPLKRQF 790 800 810 820 830 840 PSVVVTGHVL RELKQVSAGS ECLSRPWRAR AKSDLQLGGT ENFATLPSTR PRAQALSGVL 850 860 870 880 890 900 WRLQQVEEKI LQKRAQNLAN REFHTKNIKE KAAHLASMFG HGDFPQNKLL SKGLSHTHPP 910 920 930 940 950 960 SPPSRLPSPD PAASSSPSTV DSASPARKLT VGKVSSGIGA AAEVLVNLYM NDHRPKAQAT 970 980 990 1000 1010 1020 SPDLESMRKS FPLNLGGSDT CYFCKKRVYV MERLSAEGHF FHRECFRCSI CATTLRLAAY 1030 1040 1050 1060 1070 1080 TFDCDEGKFY CKPHFIHCKT NSKQRKRRAE LKQQREEEAT WQEQEAPRRD TPTESSCAVA 1090 1100 AIGTLEGSPP VHFSLPVLHP LLG
	Isoform 4 [UniParc]. Checksum: D8B4E819D43FDA7E	FASTA	976	111,652
	10 20 30 40 50 60 MGENEDEKQA QAGQVFENFV QASTCKGTLQ AFNILTRHLD LDPLDHRNFY SKLKSKVTTW 70 80 90 100 110 120 KAKALWYKLD KRGSHKEYKR GKSCTNTKCL IVGGGPCGLR TAIELAYLGA KVVVVEKRDS 130 140 150 160 170 180 FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC AGSIDHISIR QLQLILFKVA LMLGVEIHVN 190 200 210 220 230 240 VEFVKVLEPP EDQENQKIGW RAEFLPTDHS LSEFEFDVII GADGRRNTLE GFRRKEFRGK 250 260 270 280 290 300 LAIAITANFI NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDCTHYFV 310 320 330 340 350 360 MTAKKQSLLD KGVIINDYID TEMLLCAENV NQDNLLSYAR EAADFATNYQ LPSLDFAMNH 370 380 390 400 410 420 YGQPDVAMFD FTCMYASENA ALVRERQAHQ LLVALVGDSL LEPFWPMGTG CARGFLAAFD 430 440 450 460 470 480 TAWMVKSWNQ GTPPLELLAE RESLYRLLPQ TTPENINKNF EQYTLDPGTR YPNLNSHCVR 490 500 510 520 530 540 PHQVKHLYIT KELEHYPLER LGSVRRSVNL SRKESDIRPS KLLTWCQQQT EGYQHVNVTD 550 560 570 580 590 600 LTTSWRSGLA LCAIIHRFRP ELINFDSLNE DDAVENNQLA FDVAEREFGI PPVTTGKEMA 610 620 630 640 650 660 SAQEPDKLSM VMYLSKFYEL FRGTPLRPVD SWRKNYGENA DLSLAKSSIS NNYLNLTFPR 670 680 690 700 710 720 KRTPRVDGQT GENDMNKRRR KGFTNLDEPS NFSSRSLGSN QECGSSKEGG NQNKVKSMAN 730 740 750 760 770 780 QLLAKFEEST RNPSLMKQEK KSPSGFHFHP SHLRTVHPQE SMRKSFPLNL GGSDTCYFCK 790 800 810 820 830 840 KRVYVMERLS AEGHFFHREC FRCSICATTL RLAAYTFDCD EGKFYCKPHF IHCKTNSKQR 850 860 870 880 890 900 KRRAELKQQR EEEATWQEQE APRRDTPTES SCAVAAIGTL EGSPPGISTS FFRKVLGWPL 910 920 930 940 950 960 RLPRDLCNWM QGLLQAAGLH IRDNAYNYCY MYELLSLGLP LLWAFSEVLA AMYRESEGSL 970 ESICNWVLRC FPVKLR
	Isoform 5 [UniParc]. Checksum: 5CB357AB0F7D4B9E	FASTA	934	106,211
	10 20 30 40 50 60 MGENEDEKQA QAGQVFENFV QASTCKGTLQ AFNILTRHLD LDPLDHRNFY SKLKSKVTTW 70 80 90 100 110 120 KAKALWYKLD KRGSHKEYKR GKSCTNTKCL IVGGGPCGLR TAIELAYLGA KVVVVEKRDS 130 140 150 160 170 180 FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC AGSIDHISIR QLQLILFKVA LMLGVEIHVN 190 200 210 220 230 240 VEFVKVLEPP EDQENQKIGW RAEFLPTDHS LSEFEFDVII GADGRRNTLE GFRRKEFRGK 250 260 270 280 290 300 LAIAITANFI NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDCTHYFV 310 320 330 340 350 360 MTAKKQSLLD KGVIINDYID TEMLLCAENV NQDNLLSYAR EAADFATNYQ LPSLDFAMNH 370 380 390 400 410 420 YGQPDVAMFD FTCMYASENA ALVRERQAHQ LLVALVGDSL LEPFWPMGTG CARGFLAAFD 430 440 450 460 470 480 TAWMVKSWNQ GTPPLELLAE RESLYRLLPQ TTPENINKNF EQYTLDPGTR YPNLNSHCVR 490 500 510 520 530 540 PHQVKHLYIT KELEHYPLER LGSVRRSVNL SRKESDIRPS KLLTWCQQQT EGYQHVNVTD 550 560 570 580 590 600 LTTSWRSGLA LCAIIHRFRP ELINFDSLNE DDAVENNQLA FDVAEREFGI PPVTTGKEMA 610 620 630 640 650 660 SAQEPDKLSM VMYLSKFYEL FRGTPLRPVD SWRKNYGENA DLSLAKSSIS NNYLNLTFPR 670 680 690 700 710 720 KRTPRVDGQT GENDMNKRRR KGFTNLDEPS NFSSRSLGSN QECGSSKEGG NQNKVKSMAN 730 740 750 760 770 780 QLLAKFEEST RNPSLMKQEK KSPSGFHFHP SHLRTVHPQL TVGKVSSGIG AAAEVLVNLY 790 800 810 820 830 840 MNDHRPKAQA TSPDLESMRK SFPLNLGGSD TCYFCKKRVY VMERLSAEGH FFHRECFRCS 850 860 870 880 890 900 ICATTLRLAA YTFDCDEGKF YCKPHFIHCK TNSKQRKRRA ELKQQREEEA TWQEQEAPRR 910 920 930 DTPTESSCAV AAIGTLEGSP PVHFSLPVLH PLLG
	Isoform 6 [UniParc]. Checksum: 583E6EF7AC29A939	FASTA	955	109,216
	10 20 30 40 50 60 MGENEDEKQA QAGQVFENFV QASTCKGTLQ AFNILTRHLD LDPLDHRNFY SKLKSKVTTW 70 80 90 100 110 120 KAKALWYKLD KRGSHKEYKR GKSCTNTKCL IVGGGPCGLR TAIELAYLGA KVVVVEKRDS 130 140 150 160 170 180 FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC AGSIDHISIR QLQLILFKVA LMLGVEIHVN 190 200 210 220 230 240 VEFVKVLEPP EDQENQKIGW RAEFLPTDHS LSEFEFDVII GADGRRNTLE GFRRKEFRGK 250 260 270 280 290 300 LAIAITANFI NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDCTHYFV 310 320 330 340 350 360 MTAKKQSLLD KGVIINDYID TEMLLCAENV NQDNLLSYAR EAADFATNYQ LPSLDFAMNH 370 380 390 400 410 420 YGQPDVAMFD FTCMYASENA ALVRERQAHQ LLVALVGDSL LEPFWPMGTG CARGFLAAFD 430 440 450 460 470 480 TAWMVKSWNQ GTPPLELLAE RESLYRLLPQ TTPENINKNF EQYTLDPGTR YPNLNSHCVR 490 500 510 520 530 540 PHQVKHLYIT KELEHYPLER LGSVRRSVNL SRKESDIRPS KLLTWCQQQT EGYQHVNVTD 550 560 570 580 590 600 LTTSWRSGLA LCAIIHRFRP ELINFDSLNE DDAVENNQLA FDVAEREFGI PPVTTGKEMA 610 620 630 640 650 660 SAQEPDKLSM VMYLSKFYEL FRGTPLRPVD SWRKNYGENA DLSLAKSSIS NNYLNLTFPR 670 680 690 700 710 720 KRTPRVDGQT GENDMNKRRR KGFTNLDEPS NFSSRSLGSN QECGSSKEGG NQNKVKSMAN 730 740 750 760 770 780 QLLAKFEEST RNPSLMKQES MRKSFPLNLG GSDTCYFCKK RVYVMERLSA EGHFFHRECF 790 800 810 820 830 840 RCSICATTLR LAAYTFDCDE GKFYCKPHFI HCKTNSKQRK RRAELKQQRE EEATWQEQEA 850 860 870 880 890 900 PRRDTPTESS CAVAAIGTLE GSPPGISTSF FRKVLGWPLR LPRDLCNWMQ GLLQAAGLHI 910 920 930 940 950 RDNAYNYCYM YELLSLGLPL LWAFSEVLAA MYRESEGSLE SICNWVLRCF PVKLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of novel molecules, MICAL2-PV (MICAL2 prostate cancer variants), increases with high Gleason score and prostate cancer progression." Ashida S., Furihata M., Katagiri T., Tamura K., Anazawa Y., Yoshioka H., Miki T., Fujioka T., Shuin T., Nakamura Y., Nakagawa H. Clin. Cancer Res. 12:2767-2773(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4 AND 6). Tissue: Prostate.
[2]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5), VARIANT PRO-1106. Tissue: Brain and Testis.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon endothelium and Endometrium.
[5]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[8]	"MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion." Terman J.R., Mao T., Pasterkamp R.J., Yu H.-H., Kolodkin A.L. Cell 109:887-900(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE STRUCTURE.
[9]	"The MICAL proteins and rab1: a possible link to the cytoskeleton?" Fischer J., Weide T., Barnekow A. Biochem. Biophys. Res. Commun. 328:415-423(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RAB1B.
[10]	"Actin stimulates reduction of the MICAL-2 monooxygenase domain." McDonald C.A., Liu Y.Y., Palfey B.A. Biochemistry 52:6076-6084(2013) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Redox modification of nuclear actin by MICAL-2 regulates SRF signaling." Lundquist M.R., Storaska A.J., Liu T.C., Larsen S.D., Evans T., Neubig R.R., Jaffrey S.R. Cell 156:563-576(2014) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, MUTAGENESIS OF GLY-95 AND 677-LYS--LYS-681, ENZYME REGULATION.
[12]	"Solution structure of the CH domain from human MICAL-2." RIKEN structural genomics initiative (RSGI) Submitted (JUL-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 511-629.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB110785 mRNA. Translation: BAD83656.1.
AB110786 mRNA. Translation: BAD83657.1.
AB018293 mRNA. Translation: BAA34470.2. Different initiation.
AK294845 mRNA. Translation: BAG57951.1.
AK302893 mRNA. Translation: BAH13835.1.
BX538021 mRNA. Translation: CAD97967.1.
BX641163 mRNA. Translation: CAE46072.1.
AC025106 Genomic DNA. No translation available.
AC079329 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68528.1.
CH471064 Genomic DNA. Translation: EAW68529.1.
CH471064 Genomic DNA. Translation: EAW68531.1.
BC044577 mRNA. Translation: AAH44577.1.
BK000462 mRNA. Translation: DAA01341.1.

CCDS

CCDS60726.1. [O94851-3]
CCDS60727.1. [O94851-5]
CCDS60728.1. [O94851-4]
CCDS7809.1. [O94851-1]

RefSeq

NP_001269592.1. NM_001282663.1. [O94851-1]
NP_001269593.1. NM_001282664.1. [O94851-3]
NP_001269594.1. NM_001282665.1. [O94851-5]
NP_001269595.1. NM_001282666.1. [O94851-4]
NP_001269596.1. NM_001282667.1. [O94851-6]
NP_055447.1. NM_014632.3. [O94851-1]
XP_005253306.1. XM_005253249.1. [O94851-3]

UniGene

Hs.501928.

3D structure databases

PDBe
RCSB-PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E9K	NMR	-	A	516-629	[»]

ProteinModelPortal

O94851.

SMR

O94851. Positions 16-488, 511-629, 974-1056.

ModBase

Search...

MobiDB

Search...

Protein-protein interaction databases

BioGrid

115003. 1 interaction.

IntAct

O94851. 1 interaction.

STRING

9606.ENSP00000256194.

PTM databases

PhosphoSite

O94851.

Proteomic databases

MaxQB

O94851.

PaxDb

O94851.

PRIDE

O94851.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000256194; ENSP00000256194; ENSG00000133816. [O94851-1]
ENST00000342902; ENSP00000344894; ENSG00000133816. [O94851-3]
ENST00000379612; ENSP00000368932; ENSG00000133816. [O94851-4]
ENST00000527546; ENSP00000433965; ENSG00000133816. [O94851-5]
ENST00000537344; ENSP00000441689; ENSG00000133816. [O94851-5]

GeneID

9645.

KEGG

hsa:9645.

UCSC

uc001mjz.3. human. [O94851-1]
uc001mkb.3. human. [O94851-4]
uc001mkc.3. human. [O94851-6]
uc001mkd.3. human. [O94851-2]
uc010rch.1. human. [O94851-5]
uc010rci.2. human. [O94851-3]

Organism-specific databases

CTD

9645.

GeneCards

GC11P012115.

HGNC

HGNC:24693. MICAL2.

HPA

HPA030437.

MIM

608881. gene.

neXtProt

NX_O94851.

PharmGKB

PA142671453.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5069.

HOGENOM

HOG000047263.

HOVERGEN

HBG052474.

InParanoid

O94851.

OMA

CSVCATT.

OrthoDB

EOG769ZHM.

PhylomeDB

O94851.

TreeFam

TF324129.

Gene expression databases

ArrayExpress

O94851.

Bgee

O94851.

CleanEx

HS_MICAL2.

Genevestigator

O94851.

Family and domain databases

Gene3D

1.10.418.10. 1 hit.
2.10.110.10. 1 hit.

InterPro

IPR001715. CH-domain.
IPR002938. mOase_FAD-bd.
IPR003042. Rng_hydrolase-like.
IPR001781. Znf_LIM.
[Graphical view]

Pfam

PF00307. CH. 1 hit.
PF01494. FAD_binding_3. 1 hit.
PF00412. LIM. 1 hit.
[Graphical view]

PRINTS

PR00420. RNGMNOXGNASE.

SMART

SM00033. CH. 1 hit.
SM00132. LIM. 1 hit.
[Graphical view]

SUPFAM

SSF47576. SSF47576. 1 hit.

PROSITE

PS50021. CH. 1 hit.
PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

MICAL2. human.

EvolutionaryTrace

O94851.

GeneWiki

MICAL2.

GenomeRNAi

9645.

NextBio

36203.

PRO

O94851.

SOURCE

Search...

Entry information

Entry name

MICA2_HUMAN

Accession

Primary (citable) accession number: O94851
Secondary accession number(s): B4DGZ0 , B7Z849, D3DQW5, G3XAC8, Q5KTR3, Q5KTR4, Q7Z3A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 1999
Last modified:	July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.