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O94851 (MICA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein-methionine sulfoxide oxidase MICAL2

EC=1.14.13.-
Alternative name(s):
Molecule interacting with CasL protein 2
Short name=MICAL-2
Gene names
Name:MICAL2
Synonyms:KIAA0750, MICAL2PV1, MICAL2PV2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1124 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization By similarity.

Catalytic activity

[protein]-methionine + NADPH + O2 = [protein]-methionine-sulfoxide + NADP+ + H2O.

Cofactor

FAD By similarity.

Subunit structure

Interacts with VIM and PLXNA4 By similarity. Interacts with RAB1B. Ref.9

Subcellular location

Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity.

Sequence similarities

Belongs to the Mical family.

Contains 1 CH (calponin-homology) domain.

Contains 1 LIM zinc-binding domain.

Sequence caution

The sequence BAA34470.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O94851-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O94851-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-171: Missing.
     740-986: Missing.
     1112-1124: VHFSLPVLHPLLG → GISTSFFRKV...VLRCFPVKLR
Isoform 3 (identifier: O94851-3)

The sequence of this isoform differs from the canonical sequence as follows:
     929-949: Missing.
Isoform 4 (identifier: O94851-4)

The sequence of this isoform differs from the canonical sequence as follows:
     739-928: Missing.
     950-985: Missing.
     1112-1124: VHFSLPVLHPLLG → GISTSFFRKV...VLRCFPVKLR
Isoform 5 (identifier: O94851-5)

The sequence of this isoform differs from the canonical sequence as follows:
     739-928: Missing.
Isoform 6 (identifier: O94851-6)

The sequence of this isoform differs from the canonical sequence as follows:
     739-985: Missing.
     1112-1124: VHFSLPVLHPLLG → GISTSFFRKV...VLRCFPVKLR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11241124Protein-methionine sulfoxide oxidase MICAL2
PRO_0000075844

Regions

Domain516 – 619104CH
Domain1000 – 106263LIM zinc-binding
Nucleotide binding97 – 12529FAD By similarity
Region2 – 494493Monooxygenase domain By similarity

Sites

Binding site971FAD By similarity
Binding site1161FAD By similarity
Binding site1181FAD By similarity
Binding site1231FAD By similarity
Binding site1251FAD By similarity
Binding site3981FAD By similarity

Natural variations

Alternative sequence1 – 171171Missing in isoform 2.
VSP_009859
Alternative sequence739 – 985247Missing in isoform 6.
VSP_042593
Alternative sequence739 – 928190Missing in isoform 4 and isoform 5.
VSP_042594
Alternative sequence740 – 986247Missing in isoform 2.
VSP_009860
Alternative sequence929 – 94921Missing in isoform 3.
VSP_042595
Alternative sequence950 – 98536Missing in isoform 4.
VSP_042596
Alternative sequence1112 – 112413VHFSL…HPLLG → GISTSFFRKVLGWPLRLPRD LCNWMQGLLQAAGLHIRDNA YNYCYMYELLSLGLPLLWAF SEVLAAMYRESEGSLESICN WVLRCFPVKLR in isoform 2, isoform 4 and isoform 6.
VSP_009861
Natural variant1451F → L.
Corresponds to variant rs2706656 [ dbSNP | Ensembl ].
VAR_050155
Natural variant2201I → V.
Corresponds to variant rs2306727 [ dbSNP | Ensembl ].
VAR_021992
Natural variant6871D → E.
Corresponds to variant rs3794084 [ dbSNP | Ensembl ].
VAR_050156
Natural variant10891R → Q.
Corresponds to variant rs2270515 [ dbSNP | Ensembl ].
VAR_020257
Natural variant11061L → P. Ref.3
Corresponds to variant rs1027335 [ dbSNP | Ensembl ].
VAR_024523
Natural variant11101P → S.
Corresponds to variant rs35518829 [ dbSNP | Ensembl ].
VAR_050157

Experimental info

Sequence conflict191F → S in BAG57951. Ref.3
Sequence conflict2081D → G in CAD97967. Ref.4
Sequence conflict2961T → A in CAD97967. Ref.4
Sequence conflict5291Q → H in BAG57951. Ref.3
Sequence conflict5631I → V in BAG57951. Ref.3
Sequence conflict6311S → Y in CAD97967. Ref.4

Secondary structure

................... 1124
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 1C276C42D17B64DF

FASTA1,124126,689
        10         20         30         40         50         60 
MGENEDEKQA QAGQVFENFV QASTCKGTLQ AFNILTRHLD LDPLDHRNFY SKLKSKVTTW 

        70         80         90        100        110        120 
KAKALWYKLD KRGSHKEYKR GKSCTNTKCL IVGGGPCGLR TAIELAYLGA KVVVVEKRDS 

       130        140        150        160        170        180 
FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC AGSIDHISIR QLQLILFKVA LMLGVEIHVN 

       190        200        210        220        230        240 
VEFVKVLEPP EDQENQKIGW RAEFLPTDHS LSEFEFDVII GADGRRNTLE GFRRKEFRGK 

       250        260        270        280        290        300 
LAIAITANFI NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDCTHYFV 

       310        320        330        340        350        360 
MTAKKQSLLD KGVIINDYID TEMLLCAENV NQDNLLSYAR EAADFATNYQ LPSLDFAMNH 

       370        380        390        400        410        420 
YGQPDVAMFD FTCMYASENA ALVRERQAHQ LLVALVGDSL LEPFWPMGTG CARGFLAAFD 

       430        440        450        460        470        480 
TAWMVKSWNQ GTPPLELLAE RESLYRLLPQ TTPENINKNF EQYTLDPGTR YPNLNSHCVR 

       490        500        510        520        530        540 
PHQVKHLYIT KELEHYPLER LGSVRRSVNL SRKESDIRPS KLLTWCQQQT EGYQHVNVTD 

       550        560        570        580        590        600 
LTTSWRSGLA LCAIIHRFRP ELINFDSLNE DDAVENNQLA FDVAEREFGI PPVTTGKEMA 

       610        620        630        640        650        660 
SAQEPDKLSM VMYLSKFYEL FRGTPLRPVD SWRKNYGENA DLSLAKSSIS NNYLNLTFPR 

       670        680        690        700        710        720 
KRTPRVDGQT GENDMNKRRR KGFTNLDEPS NFSSRSLGSN QECGSSKEGG NQNKVKSMAN 

       730        740        750        760        770        780 
QLLAKFEEST RNPSLMKQER RVSGIGKPVL CSSSGPPVHS CCPKPEEATP SPSPPLKRQF 

       790        800        810        820        830        840 
PSVVVTGHVL RELKQVSAGS ECLSRPWRAR AKSDLQLGGT ENFATLPSTR PRAQALSGVL 

       850        860        870        880        890        900 
WRLQQVEEKI LQKRAQNLAN REFHTKNIKE KAAHLASMFG HGDFPQNKLL SKGLSHTHPP 

       910        920        930        940        950        960 
SPPSRLPSPD PAASSSPSTV DSASPARKEK KSPSGFHFHP SHLRTVHPQL TVGKVSSGIG 

       970        980        990       1000       1010       1020 
AAAEVLVNLY MNDHRPKAQA TSPDLESMRK SFPLNLGGSD TCYFCKKRVY VMERLSAEGH 

      1030       1040       1050       1060       1070       1080 
FFHRECFRCS ICATTLRLAA YTFDCDEGKF YCKPHFIHCK TNSKQRKRRA ELKQQREEEA 

      1090       1100       1110       1120 
TWQEQEAPRR DTPTESSCAV AAIGTLEGSP PVHFSLPVLH PLLG 

« Hide

Isoform 2 [UniParc].

Checksum: 114E5873F13E5C61
Show »

FASTA78489,890
Isoform 3 [UniParc].

Checksum: EAB33EE2B2DB4BA1
Show »

FASTA1,103124,253
Isoform 4 [UniParc].

Checksum: D8B4E819D43FDA7E
Show »

FASTA976111,652
Isoform 5 [UniParc].

Checksum: 5CB357AB0F7D4B9E
Show »

FASTA934106,211
Isoform 6 [UniParc].

Checksum: 583E6EF7AC29A939
Show »

FASTA955109,216

References

« Hide 'large scale' references
[1]"Expression of novel molecules, MICAL2-PV (MICAL2 prostate cancer variants), increases with high Gleason score and prostate cancer progression."
Ashida S., Furihata M., Katagiri T., Tamura K., Anazawa Y., Yoshioka H., Miki T., Fujioka T., Shuin T., Nakamura Y., Nakagawa H.
Clin. Cancer Res. 12:2767-2773(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4 AND 6).
Tissue: Prostate.
[2]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5), VARIANT PRO-1106.
Tissue: Brain and Testis.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Colon endothelium and Endometrium.
[5]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[8]"MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion."
Terman J.R., Mao T., Pasterkamp R.J., Yu H.-H., Kolodkin A.L.
Cell 109:887-900(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE STRUCTURE.
[9]"The MICAL proteins and rab1: a possible link to the cytoskeleton?"
Fischer J., Weide T., Barnekow A.
Biochem. Biophys. Res. Commun. 328:415-423(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RAB1B.
[10]"Solution structure of the CH domain from human MICAL-2."
RIKEN structural genomics initiative (RSGI)
Submitted (JUL-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 511-629.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB110785 mRNA. Translation: BAD83656.1.
AB110786 mRNA. Translation: BAD83657.1.
AB018293 mRNA. Translation: BAA34470.2. Different initiation.
AK294845 mRNA. Translation: BAG57951.1.
AK302893 mRNA. Translation: BAH13835.1.
BX538021 mRNA. Translation: CAD97967.1.
BX641163 mRNA. Translation: CAE46072.1.
AC025106 Genomic DNA. No translation available.
AC079329 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68528.1.
CH471064 Genomic DNA. Translation: EAW68529.1.
CH471064 Genomic DNA. Translation: EAW68531.1.
BC044577 mRNA. Translation: AAH44577.1.
BK000462 mRNA. Translation: DAA01341.1.
RefSeqNP_001269592.1. NM_001282663.1.
NP_001269593.1. NM_001282664.1.
NP_001269594.1. NM_001282665.1.
NP_001269595.1. NM_001282666.1.
NP_001269596.1. NM_001282667.1.
NP_055447.1. NM_014632.3.
XP_005253304.1. XM_005253247.1.
XP_005253306.1. XM_005253249.1.
UniGeneHs.501928.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E9KNMR-A516-629[»]
ProteinModelPortalO94851.
SMRO94851. Positions 16-488, 511-629, 1000-1056.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115003. 1 interaction.
IntActO94851. 1 interaction.
STRING9606.ENSP00000256194.

PTM databases

PhosphoSiteO94851.

Proteomic databases

PaxDbO94851.
PRIDEO94851.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000256194; ENSP00000256194; ENSG00000133816. [O94851-1]
ENST00000342902; ENSP00000344894; ENSG00000133816. [O94851-3]
ENST00000379612; ENSP00000368932; ENSG00000133816. [O94851-4]
ENST00000527546; ENSP00000433965; ENSG00000133816. [O94851-5]
ENST00000537344; ENSP00000441689; ENSG00000133816. [O94851-5]
GeneID9645.
KEGGhsa:9645.
UCSCuc001mjz.3. human. [O94851-1]
uc001mkb.3. human. [O94851-4]
uc001mkc.3. human. [O94851-6]
uc001mkd.3. human. [O94851-2]
uc010rch.1. human. [O94851-5]
uc010rci.2. human. [O94851-3]

Organism-specific databases

CTD9645.
GeneCardsGC11P012115.
HGNCHGNC:24693. MICAL2.
HPAHPA030437.
MIM608881. gene.
neXtProtNX_O94851.
PharmGKBPA142671453.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5069.
HOGENOMHOG000047263.
HOVERGENHBG052474.
InParanoidO94851.
OMACSVCATT.
OrthoDBEOG769ZHM.
PhylomeDBO94851.
TreeFamTF324129.

Gene expression databases

ArrayExpressO94851.
BgeeO94851.
CleanExHS_MICAL2.
GenevestigatorO94851.

Family and domain databases

Gene3D1.10.418.10. 1 hit.
2.10.110.10. 1 hit.
InterProIPR001715. CH-domain.
IPR002938. mOase_FAD-bd.
IPR003042. Rng_hydrolase-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamPF00307. CH. 1 hit.
PF01494. FAD_binding_3. 1 hit.
PF00412. LIM. 1 hit.
[Graphical view]
PRINTSPR00420. RNGMNOXGNASE.
SMARTSM00033. CH. 1 hit.
SM00132. LIM. 1 hit.
[Graphical view]
SUPFAMSSF47576. SSF47576. 1 hit.
PROSITEPS50021. CH. 1 hit.
PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMICAL2. human.
EvolutionaryTraceO94851.
GeneWikiMICAL2.
GenomeRNAi9645.
NextBio36203.
PROO94851.
SOURCESearch...

Entry information

Entry nameMICA2_HUMAN
AccessionPrimary (citable) accession number: O94851
Secondary accession number(s): B4DGZ0 expand/collapse secondary AC list , B7Z849, D3DQW5, G3XAC8, Q5KTR3, Q5KTR4, Q7Z3A8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 1999
Last modified: March 19, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM